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Protein

Protein DENND6A

Gene

DENND6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAB14. Component of an endocytic recycling pathway that is required for the control of ADAM10 transport, shedding of N-cadherin/CDH2 by ADAM9 or ADAM10 and regulation of cell-cell junctions. Required for RAB14 recruitment to recycling endosomes.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • positive regulation of cell-cell adhesion mediated by cadherin Source: UniProtKB
  • positive regulation of GTPase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DENND6A
Alternative name(s):
DENN domain-containing protein 6A
Gene namesi
Name:DENND6A
Synonyms:FAM116A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:26635. DENND6A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • recycling endosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA144596431.

Polymorphism and mutation databases

BioMutaiDENND6A.
DMDMi74728180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Protein DENND6APRO_0000289116Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei127 – 1271PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IWF6.
MaxQBiQ8IWF6.
PaxDbiQ8IWF6.
PRIDEiQ8IWF6.

PTM databases

iPTMnetiQ8IWF6.
PhosphoSiteiQ8IWF6.

Expressioni

Gene expression databases

BgeeiQ8IWF6.
CleanExiHS_FAM116A.
ExpressionAtlasiQ8IWF6. baseline and differential.
GenevisibleiQ8IWF6. HS.

Interactioni

Protein-protein interaction databases

BioGridi128398. 14 interactions.
IntActiQ8IWF6. 2 interactions.
STRINGi9606.ENSP00000311401.

Structurei

3D structure databases

ProteinModelPortaliQ8IWF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DENND6 family.Curated

Phylogenomic databases

eggNOGiKOG2432. Eukaryota.
ENOG410XP3F. LUCA.
GeneTreeiENSGT00390000005529.
HOGENOMiHOG000000674.
HOVERGENiHBG055587.
InParanoidiQ8IWF6.
OMAiDIFRCFC.
OrthoDBiEOG78PV84.
PhylomeDBiQ8IWF6.
TreeFamiTF320228.

Family and domain databases

InterProiIPR018307. ABL9/DENND6_dom.
IPR024224. DENND6.
[Graphical view]
PANTHERiPTHR13677. PTHR13677. 1 hit.
PfamiPF09794. Avl9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IWF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRGPAGLG PGSRRPLDEA VAGAEGREAP ALVAAGGAPE DDEEDDGRGR
60 70 80 90 100
GLLRWDSFSA WLHCVCVVGF DLELGQAVEV IYPQHSKLTD REKTNICYLS
110 120 130 140 150
FPDSNSGCLG DTQFCFRFRQ SSGRRVSLHC LLDQFDKDLP VYLKKDPAYF
160 170 180 190 200
YGYVYFRQVR DKTLKRGYFQ KSLVLISKLP YIHFFHTVLK QIAPEYFEKN
210 220 230 240 250
EPYLEAACND VDRWPAPVPG KTLHLPIMGV VMKVRIPTCH DKPGTTQIVQ
260 270 280 290 300
LTQQVDTNIS VILPTVHEVD IFRCFCPVFL HSQMLWELVL LGEPLVVMAP
310 320 330 340 350
SPSESSETVL ALVNCISPLK YFSDFRPYFT IHDSEFKEYT TRTQAPPSVI
360 370 380 390 400
LGVTNPFFAK TLQHWPHIIR IGDLKPTGEI PKQVKVKKLK NLKTLDSKPG
410 420 430 440 450
VYTSYKPYLN RDEEIIKQLQ KGVQQKRPSE AQSVILRRYF LELTQSFIIP
460 470 480 490 500
LERYVASLMP LQKSISPWKS PPQLRQFLPE EFMKTLEKTG PQLTSRIKGD
510 520 530 540 550
WIGLYRHFLK SPNFDGWFKT RRKEMTQKLE ALHLEALCEE DLLLWIQKHT
560 570 580 590 600
EVETVDLVLK LKNKLLQADR EHLPVKPDTM EKLRTHIDAI ILALPEDLQG

ILLKTGMT
Length:608
Mass (Da):69,575
Last modified:March 1, 2003 - v1
Checksum:iA0DFC75B7E4796BF
GO

Sequence cautioni

The sequence BAB84982.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC03852.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti229 – 2291G → A in BAB84982 (PubMed:12693554).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074156 mRNA. Translation: BAB84982.1. Different initiation.
AC114480 Genomic DNA. No translation available.
BC040291 mRNA. Translation: AAH40291.1.
BC054343 mRNA. Translation: AAH54343.1.
AK092288 mRNA. Translation: BAC03852.1. Different initiation.
CCDSiCCDS33773.1.
RefSeqiNP_689891.1. NM_152678.2.
UniGeneiHs.91085.

Genome annotation databases

EnsembliENST00000311128; ENSP00000311401; ENSG00000174839.
GeneIDi201627.
KEGGihsa:201627.
UCSCiuc003dja.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074156 mRNA. Translation: BAB84982.1. Different initiation.
AC114480 Genomic DNA. No translation available.
BC040291 mRNA. Translation: AAH40291.1.
BC054343 mRNA. Translation: AAH54343.1.
AK092288 mRNA. Translation: BAC03852.1. Different initiation.
CCDSiCCDS33773.1.
RefSeqiNP_689891.1. NM_152678.2.
UniGeneiHs.91085.

3D structure databases

ProteinModelPortaliQ8IWF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128398. 14 interactions.
IntActiQ8IWF6. 2 interactions.
STRINGi9606.ENSP00000311401.

PTM databases

iPTMnetiQ8IWF6.
PhosphoSiteiQ8IWF6.

Polymorphism and mutation databases

BioMutaiDENND6A.
DMDMi74728180.

Proteomic databases

EPDiQ8IWF6.
MaxQBiQ8IWF6.
PaxDbiQ8IWF6.
PRIDEiQ8IWF6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000311128; ENSP00000311401; ENSG00000174839.
GeneIDi201627.
KEGGihsa:201627.
UCSCiuc003dja.4. human.

Organism-specific databases

CTDi201627.
GeneCardsiDENND6A.
H-InvDBHIX0003395.
HIX0023340.
HGNCiHGNC:26635. DENND6A.
neXtProtiNX_Q8IWF6.
PharmGKBiPA144596431.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2432. Eukaryota.
ENOG410XP3F. LUCA.
GeneTreeiENSGT00390000005529.
HOGENOMiHOG000000674.
HOVERGENiHBG055587.
InParanoidiQ8IWF6.
OMAiDIFRCFC.
OrthoDBiEOG78PV84.
PhylomeDBiQ8IWF6.
TreeFamiTF320228.

Miscellaneous databases

GenomeRNAii201627.
PROiQ8IWF6.

Gene expression databases

BgeeiQ8IWF6.
CleanExiHS_FAM116A.
ExpressionAtlasiQ8IWF6. baseline and differential.
GenevisibleiQ8IWF6. HS.

Family and domain databases

InterProiIPR018307. ABL9/DENND6_dom.
IPR024224. DENND6.
[Graphical view]
PANTHERiPTHR13677. PTHR13677. 1 hit.
PfamiPF09794. Avl9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones."
    Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N., Ohara O.
    DNA Res. 10:49-57(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis and Uterus.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 177-608.
    Tissue: Tongue.
  5. Cited for: IDENTIFICATION.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells."
    Linford A., Yoshimura S., Nunes Bastos R., Langemeyer L., Gerondopoulos A., Rigden D.J., Barr F.A.
    Dev. Cell 22:952-966(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDEN6A_HUMAN
AccessioniPrimary (citable) accession number: Q8IWF6
Secondary accession number(s): Q7Z5T4, Q8N235, Q8TEG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Identified as having similarity to the core DENN family and referred to as DENN6A (PubMed:21330364). Prediction methods do not indicate a DENN domain for this sequence and, the exact role of the DENN or the DENN-like domain in GEF activity needs to be clarified.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.