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Protein

Pleckstrin homology domain-containing family M member 2

Gene

PLEKHM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation (PubMed:15905402). May play a role in lysosomes movement and localization at the cell periphery (PubMed:25898167).2 Publications

GO - Molecular functioni

  • kinesin binding Source: UniProtKB

GO - Biological processi

  • Golgi organization Source: UniProtKB
  • lysosome localization Source: UniProtKB
  • positive regulation of membrane tubulation Source: AgBase
  • regulation of protein localization Source: AgBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family M member 2
Short name:
PH domain-containing family M member 2
Alternative name(s):
Salmonella-induced filaments A and kinesin-interacting protein
Short name:
SifA and kinesin-interacting protein
Gene namesi
Name:PLEKHM2
Synonyms:KIAA0842, SKIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29131. PLEKHM2.

Subcellular locationi

GO - Cellular componenti

  • endosome membrane Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi828G → D: Loss of interaction with sifA. 1 Publication1
Mutagenesisi830R → D: Loss of interaction with sifA. 1 Publication1
Mutagenesisi831R → A: Alters interaction with sifA. 1 Publication1
Mutagenesisi869C → D: Loss of interaction with sifA. 1 Publication1

Organism-specific databases

DisGeNETi23207.
OpenTargetsiENSG00000116786.
PharmGKBiPA134888781.

Polymorphism and mutation databases

BioMutaiPLEKHM2.
DMDMi160419243.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003094551 – 1019Pleckstrin homology domain-containing family M member 2Add BLAST1019

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei441PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8IWE5.
MaxQBiQ8IWE5.
PaxDbiQ8IWE5.
PeptideAtlasiQ8IWE5.
PRIDEiQ8IWE5.

PTM databases

iPTMnetiQ8IWE5.
PhosphoSitePlusiQ8IWE5.

Expressioni

Gene expression databases

BgeeiENSG00000116786.
ExpressionAtlasiQ8IWE5. baseline and differential.
GenevisibleiQ8IWE5. HS.

Organism-specific databases

HPAiHPA032304.

Interactioni

Subunit structurei

Interacts with KIF5B (PubMed:15905402). Interacts with BORCS5 (PubMed:25898167).2 Publications
(Microbial infection) Interacts with the S.typhimurium sifA protein; required for S.typhimurium infection.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAB9AP511514EBI-726484,EBI-4401353
sifAA0A0F6B0633EBI-726484,EBI-11477981From a different organism.
sifAQ560613EBI-726484,EBI-10765408From a different organism.

GO - Molecular functioni

  • kinesin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116814. 3 interactors.
DIPiDIP-46410N.
IntActiQ8IWE5. 4 interactors.
STRINGi9606.ENSP00000364956.

Structurei

Secondary structure

11019
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni210 – 212Combined sources3
Beta strandi774 – 780Combined sources7
Beta strandi787 – 789Combined sources3
Beta strandi793 – 800Combined sources8
Beta strandi803 – 811Combined sources9
Beta strandi816 – 820Combined sources5
Beta strandi826 – 831Combined sources6
Beta strandi840 – 845Combined sources6
Beta strandi851 – 854Combined sources4
Helixi858 – 872Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXBX-ray2.60B773-884[»]
3HW2X-ray3.30B771-875[»]
3ZFWX-ray2.90X/Y203-212[»]
ProteinModelPortaliQ8IWE5.
SMRiQ8IWE5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IWE5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 158RUNPROSITE-ProRule annotationAdd BLAST123
Domaini771 – 873PHPROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 310Interaction with KIF5B1 PublicationAdd BLAST310
Regioni762 – 885Interaction with sifAAdd BLAST124

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi317 – 320Poly-Lys4
Compositional biasi323 – 326Poly-Lys4
Compositional biasi506 – 511Poly-Gly6
Compositional biasi593 – 596Poly-Leu4

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 RUN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFVN. Eukaryota.
ENOG410ZZUG. LUCA.
GeneTreeiENSGT00390000015175.
HOVERGENiHBG059073.
InParanoidiQ8IWE5.
KOiK15348.
OMAiDPSTWCS.
OrthoDBiEOG091G013Y.
PhylomeDBiQ8IWE5.
TreeFamiTF332641.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50826. RUN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IWE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGEVKDRI LENISLSVKK LQSYFAACED EIPAIRNHDK VLQRLCEHLD
60 70 80 90 100
HALLYGLQDL SSGYWVLVVH FTRREAIKQI EVLQHVATNL GRSRAWLYLA
110 120 130 140 150
LNENSLESYL RLFQENLGLL HKYYVKNALV CSHDHLTLFL TLVSGLEFIR
160 170 180 190 200
FELDLDAPYL DLAPYMPDYY KPQYLLDFED RLPSSVHGSD SLSLNSFNSV
210 220 230 240 250
TSTNLEWDDS AIAPSSEDYD FGDVFPAVPS VPSTDWEDGD LTDTVSGPRS
260 270 280 290 300
TASDLTSSKA STRSPTQRQN PFNEEPAETV SSSDTTPVHT TSQEKEEAQA
310 320 330 340 350
LDPPDACTEL EVIRVTKKKK IGKKKKSRSD EEASPLHPAC SQKKCAKQGD
360 370 380 390 400
GDSRNGSPSL GRDSPDTMLA SPQEEGEGPS STTESSERSE PGLLIPEMKD
410 420 430 440 450
TSMERLGQPL SKVIDQLNGQ LDPSTWCSRA EPPDQSFRTG SPGDAPERPP
460 470 480 490 500
LCDFSEGLSA PMDFYRFTVE SPSTVTSGGG HHDPAGLGQP LHVPSSPEAA
510 520 530 540 550
GQEEEGGGGE GQTPRPLEDT TREAQELEAQ LSLVREGPVS EPEPGTQEVL
560 570 580 590 600
CQLKRDQPSP CLSSAEDSGV DEGQGSPSEM VHSSEFRVDN NHLLLLMIHV
610 620 630 640 650
FRENEEQLFK MIRMSTGHME GNLQLLYVLL TDCYVYLLRK GATEKPYLVE
660 670 680 690 700
EAVSYNELDY VSVGLDQQTV KLVCTNRRKQ FLLDTADVAL AEFFLASLKS
710 720 730 740 750
AMIKGCREPP YPSILTDATM EKLALAKFVA QESKCEASAV TVRFYGLVHW
760 770 780 790 800
EDPTDESLGP TPCHCSPPEG TITKEGMLHY KAGTSYLGKE HWKTCFVVLS
810 820 830 840 850
NGILYQYPDR TDVIPLLSVN MGGEQCGGCR RANTTDRPHA FQVILSDRPC
860 870 880 890 900
LELSAESEAE MAEWMQHLCQ AVSKGVIPQG VAPSPCIPCC LVLTDDRLFT
910 920 930 940 950
CHEDCQTSFF RSLGTAKLGD ISAVSTEPGK EYCVLEFSQD SQQLLPPWVI
960 970 980 990 1000
YLSCTSELDR LLSALNSGWK TIYQVDLPHT AIQEASNKKK FEDALSLIHS
1010
AWQRSDSLCR GRASRDPWC
Length:1,019
Mass (Da):112,780
Last modified:November 13, 2007 - v2
Checksum:iFFD857F4C11BC0DB
GO

Sequence cautioni

The sequence AAH40441 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA74865 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti600 – 602VFR → ASG in AAH30545 (PubMed:15489334).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03695032I → T.Corresponds to variant rs12091750dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020649 mRNA. Translation: BAA74865.1. Different initiation.
AL450998, AL121992, AL606758 Genomic DNA. Translation: CAH70859.1.
AL606758, AL121992, AL450998 Genomic DNA. Translation: CAH72017.1.
AL121992, AL450998, AL606758 Genomic DNA. Translation: CAI22373.1.
CH471167 Genomic DNA. Translation: EAW51745.1.
BC008002 mRNA. Translation: AAH08002.1.
BC016488 mRNA. Translation: AAH16488.1.
BC030545 mRNA. Translation: AAH30545.1.
BC040441 mRNA. Translation: AAH40441.1. Different initiation.
BC042103 mRNA. Translation: AAH42103.1.
AL137297 mRNA. Translation: CAB70684.1.
CCDSiCCDS44063.1.
PIRiT46361.
RefSeqiNP_055979.2. NM_015164.2.
UniGeneiHs.646775.

Genome annotation databases

EnsembliENST00000375799; ENSP00000364956; ENSG00000116786.
GeneIDi23207.
KEGGihsa:23207.
UCSCiuc010obo.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020649 mRNA. Translation: BAA74865.1. Different initiation.
AL450998, AL121992, AL606758 Genomic DNA. Translation: CAH70859.1.
AL606758, AL121992, AL450998 Genomic DNA. Translation: CAH72017.1.
AL121992, AL450998, AL606758 Genomic DNA. Translation: CAI22373.1.
CH471167 Genomic DNA. Translation: EAW51745.1.
BC008002 mRNA. Translation: AAH08002.1.
BC016488 mRNA. Translation: AAH16488.1.
BC030545 mRNA. Translation: AAH30545.1.
BC040441 mRNA. Translation: AAH40441.1. Different initiation.
BC042103 mRNA. Translation: AAH42103.1.
AL137297 mRNA. Translation: CAB70684.1.
CCDSiCCDS44063.1.
PIRiT46361.
RefSeqiNP_055979.2. NM_015164.2.
UniGeneiHs.646775.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXBX-ray2.60B773-884[»]
3HW2X-ray3.30B771-875[»]
3ZFWX-ray2.90X/Y203-212[»]
ProteinModelPortaliQ8IWE5.
SMRiQ8IWE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116814. 3 interactors.
DIPiDIP-46410N.
IntActiQ8IWE5. 4 interactors.
STRINGi9606.ENSP00000364956.

PTM databases

iPTMnetiQ8IWE5.
PhosphoSitePlusiQ8IWE5.

Polymorphism and mutation databases

BioMutaiPLEKHM2.
DMDMi160419243.

Proteomic databases

EPDiQ8IWE5.
MaxQBiQ8IWE5.
PaxDbiQ8IWE5.
PeptideAtlasiQ8IWE5.
PRIDEiQ8IWE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375799; ENSP00000364956; ENSG00000116786.
GeneIDi23207.
KEGGihsa:23207.
UCSCiuc010obo.2. human.

Organism-specific databases

CTDi23207.
DisGeNETi23207.
GeneCardsiPLEKHM2.
HGNCiHGNC:29131. PLEKHM2.
HPAiHPA032304.
MIMi609613. gene.
neXtProtiNX_Q8IWE5.
OpenTargetsiENSG00000116786.
PharmGKBiPA134888781.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFVN. Eukaryota.
ENOG410ZZUG. LUCA.
GeneTreeiENSGT00390000015175.
HOVERGENiHBG059073.
InParanoidiQ8IWE5.
KOiK15348.
OMAiDPSTWCS.
OrthoDBiEOG091G013Y.
PhylomeDBiQ8IWE5.
TreeFamiTF332641.

Miscellaneous databases

EvolutionaryTraceiQ8IWE5.
GeneWikiiPLEKHM2.
GenomeRNAii23207.
PROiQ8IWE5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116786.
ExpressionAtlasiQ8IWE5. baseline and differential.
GenevisibleiQ8IWE5. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50826. RUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHM2_HUMAN
AccessioniPrimary (citable) accession number: Q8IWE5
Secondary accession number(s): O94928
, Q5VT65, Q6NUH9, Q7L8G1, Q8IVT7, Q8N2T4, Q96AY0, Q9NTF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.