Q8IWE5 (PKHM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleckstrin homology domain-containing family M member 2
Short name=PH domain-containing family M member 2

Alternative name(s):
Salmonella-induced filaments A and kinesin-interacting protein
Short name=SifA and kinesin-interacting protein

Gene names

Name:	PLEKHM2
Synonyms:	KIAA0842, SKIP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1019 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation. Ref.6
Subunit structure	Interacts with KIF5B. Interacts with the S.typhimurium sifA protein; required for S.typhimurium infection. Ref.6
Subcellular location	Cytoplasm Ref.6.
Sequence similarities	Contains 1 PH domain. Contains 1 RUN domain.
Sequence caution	The sequence AAH40441.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA74865.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinesin binding Inferred from direct assay Ref.6. Source: UniProtKB phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1019

1019

Pleckstrin homology domain-containing family M member 2

PRO_0000309455

Regions

Domain

36 – 158

123

RUN

Domain

771 – 873

103

PH

Region

1 – 310

310

Interaction with KIF5B

Region

762 – 885

124

Interaction with sifA

Compositional bias

317 – 320

4

Poly-Lys

Compositional bias

323 – 326

4

Poly-Lys

Compositional bias

506 – 511

6

Poly-Gly

Compositional bias

593 – 596

4

Poly-Leu

Amino acid modifications

Modified residue

559

1

Phosphoserine By similarity

Natural variations

Natural variant

32

1

I → T.
Corresponds to variant rs12091750 [ dbSNP | Ensembl ].

VAR_036950

Experimental info

Mutagenesis

828

1

G → D: Loss of interaction with sifA. Ref.9

Mutagenesis

830

1

R → D: Loss of interaction with sifA. Ref.9

Mutagenesis

831

1

R → A: Alters interaction with sifA. Ref.9

Mutagenesis

869

1

C → D: Loss of interaction with sifA. Ref.9

Sequence conflict

600 – 602

3

VFR → ASG in AAH30545. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1019

Helix Strand Turn

Details...

Beta strand

774 – 780

7

Beta strand

787 – 789

3

Beta strand

793 – 800

8

Beta strand

803 – 811

9

Beta strand

816 – 820

5

Beta strand

826 – 831

6

Beta strand

840 – 845

6

Beta strand

851 – 854

4

Helix

858 – 872

15

Sequences

	Sequence		Length	Mass (Da)
	Q8IWE5 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: FFD857F4C11BC0DB	FASTA	1,019	112,780
	10 20 30 40 50 60 MEPGEVKDRI LENISLSVKK LQSYFAACED EIPAIRNHDK VLQRLCEHLD HALLYGLQDL 70 80 90 100 110 120 SSGYWVLVVH FTRREAIKQI EVLQHVATNL GRSRAWLYLA LNENSLESYL RLFQENLGLL 130 140 150 160 170 180 HKYYVKNALV CSHDHLTLFL TLVSGLEFIR FELDLDAPYL DLAPYMPDYY KPQYLLDFED 190 200 210 220 230 240 RLPSSVHGSD SLSLNSFNSV TSTNLEWDDS AIAPSSEDYD FGDVFPAVPS VPSTDWEDGD 250 260 270 280 290 300 LTDTVSGPRS TASDLTSSKA STRSPTQRQN PFNEEPAETV SSSDTTPVHT TSQEKEEAQA 310 320 330 340 350 360 LDPPDACTEL EVIRVTKKKK IGKKKKSRSD EEASPLHPAC SQKKCAKQGD GDSRNGSPSL 370 380 390 400 410 420 GRDSPDTMLA SPQEEGEGPS STTESSERSE PGLLIPEMKD TSMERLGQPL SKVIDQLNGQ 430 440 450 460 470 480 LDPSTWCSRA EPPDQSFRTG SPGDAPERPP LCDFSEGLSA PMDFYRFTVE SPSTVTSGGG 490 500 510 520 530 540 HHDPAGLGQP LHVPSSPEAA GQEEEGGGGE GQTPRPLEDT TREAQELEAQ LSLVREGPVS 550 560 570 580 590 600 EPEPGTQEVL CQLKRDQPSP CLSSAEDSGV DEGQGSPSEM VHSSEFRVDN NHLLLLMIHV 610 620 630 640 650 660 FRENEEQLFK MIRMSTGHME GNLQLLYVLL TDCYVYLLRK GATEKPYLVE EAVSYNELDY 670 680 690 700 710 720 VSVGLDQQTV KLVCTNRRKQ FLLDTADVAL AEFFLASLKS AMIKGCREPP YPSILTDATM 730 740 750 760 770 780 EKLALAKFVA QESKCEASAV TVRFYGLVHW EDPTDESLGP TPCHCSPPEG TITKEGMLHY 790 800 810 820 830 840 KAGTSYLGKE HWKTCFVVLS NGILYQYPDR TDVIPLLSVN MGGEQCGGCR RANTTDRPHA 850 860 870 880 890 900 FQVILSDRPC LELSAESEAE MAEWMQHLCQ AVSKGVIPQG VAPSPCIPCC LVLTDDRLFT 910 920 930 940 950 960 CHEDCQTSFF RSLGTAKLGD ISAVSTEPGK EYCVLEFSQD SQQLLPPWVI YLSCTSELDR 970 980 990 1000 1010 LLSALNSGWK TIYQVDLPHT AIQEASNKKK FEDALSLIHS AWQRSDSLCR GRASRDPWC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone, Cervix, Kidney, Ovary and Testis.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 824-1019. Tissue: Testis.
[6]	"The intracellular fate of Salmonella depends on the recruitment of kinesin." Boucrot E., Henry T., Borg J.-P., Gorvel J.-P., Meresse S. Science 308:1174-1178(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KIF5B AND SALMONELLA TYPHIMURIUM SIFA PROTEIN.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Structure and function of Salmonella SifA indicate that its interactions with SKIP, SseJ, and RhoA family GTPases induce endosomal tubulation." Ohlson M.B., Huang Z., Alto N.M., Blanc M.-P., Dixon J.E., Chai J., Miller S.I. Cell Host Microbe 4:434-446(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 773-884 IN COMPLEX WITH SALMONELLA TYPHIMURIUM SIFA PROTEIN, MUTAGENESIS OF GLY-828; ARG-830; ARG-831 AND CYS-869.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB020649 mRNA. Translation: BAA74865.1. Different initiation.
AL450998, AL121992, AL606758 Genomic DNA. Translation: CAH70859.1.
AL606758, AL121992, AL450998 Genomic DNA. Translation: CAH72017.1.
AL121992, AL450998, AL606758 Genomic DNA. Translation: CAI22373.1.
CH471167 Genomic DNA. Translation: EAW51745.1.
BC008002 mRNA. Translation: AAH08002.1.
BC016488 mRNA. Translation: AAH16488.1.
BC030545 mRNA. Translation: AAH30545.1.
BC040441 mRNA. Translation: AAH40441.1. Different initiation.
BC042103 mRNA. Translation: AAH42103.1.
AL137297 mRNA. Translation: CAB70684.1.

IPI

IPI00217283.

PIR

T46361.

RefSeq

NP_055979.2. NM_015164.2.

UniGene

Hs.646775.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CXB	X-ray	2.60	B	773-884	[»]
3HW2	X-ray	3.30	B	771-875	[»]

ProteinModelPortal

Q8IWE5.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46410N.

IntAct

Q8IWE5. 1 interaction.

PTM databases

PhosphoSite

Q8IWE5.

Polymorphism databases

DMDM

160419243.

Proteomic databases

PaxDb

Q8IWE5.

PRIDE

Q8IWE5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000375799; ENSP00000364956; ENSG00000116786.

GeneID

23207.

KEGG

hsa:23207.

UCSC

uc010obo.2. human.

Organism-specific databases

CTD

23207.

GeneCards

GC01P016008.

HGNC

HGNC:29131. PLEKHM2.

HPA

HPA032304.

MIM

609613. gene.

neXtProt

NX_Q8IWE5.

PharmGKB

PA134888781.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41387.

HOVERGEN

HBG059073.

InParanoid

Q8IWE5.

KO

K15348.

OMA

DCYVYLL.

OrthoDB

EOG4VT5WF.

Gene expression databases

ArrayExpress

Q8IWE5.

Bgee

Q8IWE5.

Genevestigator

Q8IWE5.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR004012. Run.
[Graphical view]

Pfam

PF00169. PH. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50826. RUN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PLEKHM2. human.

EvolutionaryTrace

Q8IWE5.

GenomeRNAi

23207.

NextBio

44735.

SOURCE

Search...

Entry information

Entry name

PKHM2_HUMAN

Accession

Primary (citable) accession number: Q8IWE5
Secondary accession number(s): O94928 , Q5VT65, Q6NUH9, Q7L8G1, Q8IVT7, Q8N2T4, Q96AY0, Q9NTF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.