Q8IWB7 (WDFY1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat and FYVE domain-containing protein 1

Alternative name(s):
FENS-1
Phosphoinositide-binding protein 1
WD40- and FYVE domain-containing protein 1
Zinc finger FYVE domain-containing protein 17

Gene names

Name:	WDFY1
Synonyms:	KIAA1435, WDF1, ZFYVE17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Binds PtdIns3P in vitro with high specificity over other phosphoinositides.
Subcellular location	Early endosome Ref.1.
Domain	The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions. Ref.8
Sequence similarities	Contains 1 FYVE-type zinc finger. Contains 7 WD repeats.
Sequence caution	The sequence BAA92673.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endosome
Domain	Repeat WD repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB early endosome Inferred from direct assay Ref.1. Source: UniProtKB nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	1-phosphatidylinositol binding Inferred from direct assay Ref.1. Source: UniProtKB zinc ion binding Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	WD repeat and FYVE domain-containing protein 1		PRO_0000051335
Regions
	Repeat	22 – 61	40	WD 1
	Repeat	66 – 105	40	WD 2
	Repeat	112 – 150	39	WD 3
	Repeat	153 – 192	40	WD 4
	Repeat	197 – 236	40	WD 5
	Repeat	240 – 279	40	WD 6
	Repeat	364 – 403	40	WD 7
	Zinc finger	281 – 352	72	FYVE-type
Amino acid modifications
	Modified residue	408	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q8IWB7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 25D981799D918BE3	FASTA	410	46,324
	10 20 30 40 50 60 MAAEIHSRPQ SSRPVLLSKI EGHQDAVTAA LLIPKEDGVI TASEDRTIRV WLKRDSGQYW 70 80 90 100 110 120 PSIYHTMASP CSAMAYHHDS RRIFVGQDNG AVMEFHVSED FNKMNFIKTY PAHQNRVSAI 130 140 150 160 170 180 IFSLATEWVI STGHDKCVSW MCTRSGNMLG RHFFTSWASC LQYDFDTQYA FVGDYSGQIT 190 200 210 220 230 240 LLKLEQNTCS VITTLKGHEG SVACLWWDPI QRLLFSGASD NSIIMWDIGG RKGRTLLLQG 250 260 270 280 290 300 HHDKVQSLCY LQLTRQLVSC SSDGGIAVWN MDVSREEAPQ WLESDSCQKC EQPFFWNIKQ 310 320 330 340 350 360 MWDTKTLGLR QHHCRKCGQA VCGKCSSKRS SYPVMGFEFQ VRVCDSCYDS IKDEDRTSLA 370 380 390 400 410 TFHEGKHNIS HMSMDIARGL MVTCGTDRIV KIWDMTPVVG CSLATGFSPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments." Ridley S.H., Ktistakis N., Davidson K., Anderson K.E., Manifava M., Ellson C.D., Lipp P., Bootman M., Coadwell J., Nazarian A., Erdjument-Bromage H., Tempst P., Cooper M.A., Thuring J.W.J.F., Lim Z.-Y., Holmes A.B., Stephens L.R., Hawkins P.T. J. Cell Sci. 114:3991-4000(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, BINDING TO PHOSPHOINOSITIDES.
[2]	"WD40- and FYVE-domain containing protein 1 (WDF1)." Hong W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
[8]	"Membrane insertion of the FYVE domain is modulated by pH." He J., Vora M., Haney R.M., Filonov G.S., Musselman C.A., Burd C.G., Kutateladze A.G., Verkhusha V.V., Stahelin R.V., Kutateladze T.G. Proteins 76:852-860(2009) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN FYVE-TYPE ZINC FINGER.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ310568 mRNA. Translation: CAC83947.1. AF411977 mRNA. Translation: AAL04161.1. AB037856 mRNA. Translation: BAA92673.1. Different initiation. AK022888 mRNA. Translation: BAB14294.1. AC073641 Genomic DNA. Translation: AAY14924.1. CH471063 Genomic DNA. Translation: EAW70817.1. BC040525 mRNA. Translation: AAH40525.1. BC065934 mRNA. Translation: AAH65934.1.
IPI	IPI00024283.
RefSeq	NP_065881.1. NM_020830.3.
UniGene	Hs.368359.
3D structure databases
ProteinModelPortal	Q8IWB7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000233055.
PTM databases
PhosphoSite	Q8IWB7.
Polymorphism databases
DMDM	51316866.
Proteomic databases
PaxDb	Q8IWB7.
PeptideAtlas	Q8IWB7.
PRIDE	Q8IWB7.
Protocols and materials databases
DNASU	57590.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233055; ENSP00000233055; ENSG00000085449.
GeneID	57590.
KEGG	hsa:57590.
UCSC	uc002vnq.3. human.
Organism-specific databases
CTD	57590.
GeneCards	GC02M224704.
HGNC	HGNC:20451. WDFY1.
HPA	HPA050603.
neXtProt	NX_Q8IWB7.
PharmGKB	PA134929936.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000007835.
HOVERGEN	HBG054128.
InParanoid	Q8IWB7.
OMA	SIACLWW.
OrthoDB	EOG41ZF9T.
Gene expression databases
ArrayExpress	Q8IWB7.
Bgee	Q8IWB7.
CleanEx	HS_WDFY1.
Genevestigator	Q8IWB7.
GermOnline	ENSG00000085449. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 2 hits. 3.30.40.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF01363. FYVE. 1 hit. PF00400. WD40. 4 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00064. FYVE. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57590.
NextBio	64174.

Entry information

Entry name

WDFY1_HUMAN

Accession

Primary (citable) accession number: Q8IWB7
Secondary accession number(s): Q53S17, Q9H9D5, Q9P2B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.