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Q8IWB7 (WDFY1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WD repeat and FYVE domain-containing protein 1
Alternative name(s):
FENS-1
Phosphoinositide-binding protein 1
WD40- and FYVE domain-containing protein 1
Zinc finger FYVE domain-containing protein 17
Gene names
Name:WDFY1
Synonyms:KIAA1435, WDF1, ZFYVE17
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Binds PtdIns3P in vitro with high specificity over other phosphoinositides.

Subcellular location

Early endosome Ref.1.

Domain

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions. Ref.8

Sequence similarities

Contains 1 FYVE-type zinc finger.

Contains 7 WD repeats.

Sequence caution

The sequence BAA92673.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentEndosome
   DomainRepeat
WD repeat
Zinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from direct assay Ref.1. Source: UniProtKB

early endosome

Inferred from direct assay Ref.1. Source: UniProtKB

nucleus

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_function1-phosphatidylinositol binding

Inferred from direct assay Ref.1. Source: UniProtKB

zinc ion binding

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410WD repeat and FYVE domain-containing protein 1
PRO_0000051335

Regions

Repeat22 – 6140WD 1
Repeat66 – 10540WD 2
Repeat112 – 15039WD 3
Repeat153 – 19240WD 4
Repeat197 – 23640WD 5
Repeat240 – 27940WD 6
Repeat364 – 40340WD 7
Zinc finger281 – 35272FYVE-type

Amino acid modifications

Modified residue4081Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q8IWB7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 25D981799D918BE3

FASTA41046,324
        10         20         30         40         50         60 
MAAEIHSRPQ SSRPVLLSKI EGHQDAVTAA LLIPKEDGVI TASEDRTIRV WLKRDSGQYW 

        70         80         90        100        110        120 
PSIYHTMASP CSAMAYHHDS RRIFVGQDNG AVMEFHVSED FNKMNFIKTY PAHQNRVSAI 

       130        140        150        160        170        180 
IFSLATEWVI STGHDKCVSW MCTRSGNMLG RHFFTSWASC LQYDFDTQYA FVGDYSGQIT 

       190        200        210        220        230        240 
LLKLEQNTCS VITTLKGHEG SVACLWWDPI QRLLFSGASD NSIIMWDIGG RKGRTLLLQG 

       250        260        270        280        290        300 
HHDKVQSLCY LQLTRQLVSC SSDGGIAVWN MDVSREEAPQ WLESDSCQKC EQPFFWNIKQ 

       310        320        330        340        350        360 
MWDTKTLGLR QHHCRKCGQA VCGKCSSKRS SYPVMGFEFQ VRVCDSCYDS IKDEDRTSLA 

       370        380        390        400        410 
TFHEGKHNIS HMSMDIARGL MVTCGTDRIV KIWDMTPVVG CSLATGFSPH 

« Hide

References

« Hide 'large scale' references
[1]"FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments."
Ridley S.H., Ktistakis N., Davidson K., Anderson K.E., Manifava M., Ellson C.D., Lipp P., Bootman M., Coadwell J., Nazarian A., Erdjument-Bromage H., Tempst P., Cooper M.A., Thuring J.W.J.F., Lim Z.-Y., Holmes A.B., Stephens L.R., Hawkins P.T.
J. Cell Sci. 114:3991-4000(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, BINDING TO PHOSPHOINOSITIDES.
[2]"WD40- and FYVE-domain containing protein 1 (WDF1)."
Hong W.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Skin.
[8]"Membrane insertion of the FYVE domain is modulated by pH."
He J., Vora M., Haney R.M., Filonov G.S., Musselman C.A., Burd C.G., Kutateladze A.G., Verkhusha V.V., Stahelin R.V., Kutateladze T.G.
Proteins 76:852-860(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN FYVE-TYPE ZINC-FINGER.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ310568 mRNA. Translation: CAC83947.1.
AF411977 mRNA. Translation: AAL04161.1.
AB037856 mRNA. Translation: BAA92673.1. Different initiation.
AK022888 mRNA. Translation: BAB14294.1.
AC073641 Genomic DNA. Translation: AAY14924.1.
CH471063 Genomic DNA. Translation: EAW70817.1.
BC040525 mRNA. Translation: AAH40525.1.
BC065934 mRNA. Translation: AAH65934.1.
RefSeqNP_065881.1. NM_020830.3.
UniGeneHs.368359.

3D structure databases

ProteinModelPortalQ8IWB7.
SMRQ8IWB7. Positions 16-409.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121641. 4 interactions.
MINTMINT-5004828.
STRING9606.ENSP00000233055.

PTM databases

PhosphoSiteQ8IWB7.

Polymorphism databases

DMDM51316866.

Proteomic databases

PaxDbQ8IWB7.
PeptideAtlasQ8IWB7.
PRIDEQ8IWB7.

Protocols and materials databases

DNASU57590.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233055; ENSP00000233055; ENSG00000085449.
GeneID57590.
KEGGhsa:57590.
UCSCuc002vnq.3. human.

Organism-specific databases

CTD57590.
GeneCardsGC02M224704.
HGNCHGNC:20451. WDFY1.
HPAHPA050603.
neXtProtNX_Q8IWB7.
PharmGKBPA134929936.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000007835.
HOVERGENHBG054128.
InParanoidQ8IWB7.
OMAEAPQWLD.
OrthoDBEOG7W153N.
PhylomeDBQ8IWB7.
TreeFamTF314470.

Enzyme and pathway databases

SignaLinkQ8IWB7.

Gene expression databases

ArrayExpressQ8IWB7.
BgeeQ8IWB7.
CleanExHS_WDFY1.
GenevestigatorQ8IWB7.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
3.30.40.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00064. FYVE. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi57590.
NextBio64174.
PROQ8IWB7.

Entry information

Entry nameWDFY1_HUMAN
AccessionPrimary (citable) accession number: Q8IWB7
Secondary accession number(s): Q53S17, Q9H9D5, Q9P2B3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM