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Protein

Inactive serine/threonine-protein kinase TEX14

Gene

TEX14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi233 – 241ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: InterPro
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi1.A.87.2.7. the mechanosensitive calcium channel (mca) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive serine/threonine-protein kinase TEX14
Alternative name(s):
Protein kinase-like protein SgK307
Sugen kinase 307
Testis-expressed sequence 14
Testis-expressed sequence 14 protein
Gene namesi
Name:TEX14
Synonyms:SGK307
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:11737. TEX14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi614 – 618SSPST → AAPAA in PBD-1; reduced ability to interact with PLK1. 1 Publication5
Mutagenesisi726 – 728STT → AAA in PBD-5; reduced ability to interact with PLK1. 1 Publication3

Organism-specific databases

DisGeNETi56155.
OpenTargetsiENSG00000121101.
PharmGKBiPA36454.

Polymorphism and mutation databases

BioMutaiTEX14.
DMDMi110816441.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002469951 – 1497Inactive serine/threonine-protein kinase TEX14Add BLAST1497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175PhosphoserineBy similarity1
Modified residuei437Phosphoserine; by PLK1By similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei665PhosphoserineBy similarity1
Modified residuei1106PhosphoserineBy similarity1
Modified residuei1404PhosphoserineBy similarity1
Modified residuei1459PhosphoserineBy similarity1
Modified residuei1496PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8IWB6.
PeptideAtlasiQ8IWB6.
PRIDEiQ8IWB6.

PTM databases

iPTMnetiQ8IWB6.
PhosphoSitePlusiQ8IWB6.

Expressioni

Tissue specificityi

Detected in testis.1 Publication

Gene expression databases

BgeeiENSG00000121101.
CleanExiHS_TEX14.
ExpressionAtlasiQ8IWB6. baseline and differential.
GenevisibleiQ8IWB6. HS.

Organism-specific databases

HPAiHPA030620.

Interactioni

Subunit structurei

Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP55Q53EZ43EBI-6674697,EBI-747776

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121089. 3 interactors.
IntActiQ8IWB6. 3 interactors.
STRINGi9606.ENSP00000240361.

Structurei

Secondary structure

11497
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi797 – 800Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WUTX-ray2.30C/F/I/L792-804[»]
3WUUX-ray2.90C/F/I/L791-805[»]
ProteinModelPortaliQ8IWB6.
SMRiQ8IWB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 54ANK 1Add BLAST28
Repeati55 – 84ANK 2Add BLAST30
Repeati88 – 117ANK 3Add BLAST30
Domaini227 – 518Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi795 – 801GPPX3Y7
Motifi852 – 860D-box9

Domaini

The protein kinase domain is predicted to be catalytically inactive.
The GPPX3Y motif mediates interaction with CEP55.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IHZA. Eukaryota.
ENOG410XT67. LUCA.
GeneTreeiENSGT00390000015123.
HOGENOMiHOG000059264.
HOVERGENiHBG094035.
InParanoidiQ8IWB6.
KOiK17540.
OMAiLEYMMES.
OrthoDBiEOG091G0CDS.
PhylomeDBiQ8IWB6.
TreeFamiTF328704.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRAVRLPVP CPVQLGTLRN DSLEAQLHEY VKQGNYVKVK KILKKGIYVD
60 70 80 90 100
AVNSLGQTAL FVAALLGLRK FVDVLVDYGS DPNHRCFDGS TPVHAAAFSG
110 120 130 140 150
NQWILSKLLD AGGDLRLHDE RGQNPKTWAL TAGKERSTQI VEFMQRCASH
160 170 180 190 200
MQAIIQGFSY DLLKKIDSPQ RLVYSPSWCG GLVQGNPNGS PNRLLKAGVI
210 220 230 240 250
SAQNIYSFGF GKAMPWFQFY LTGATQMAYL GSLPVIGEKE VIQADDEPTF
260 270 280 290 300
SFFSGPYMVM TNLVWNGSRV TVKELNLPTH PHCSRLRLAD LLIAEQEHSS
310 320 330 340 350
KLRHPYLLQL MAVCLSQDLE KTRLVYERIT IGTLFSVLHE RRSQFPVLHM
360 370 380 390 400
EVIVHLLLQI SDALRYLHFQ GFIHRSLSSY AVHIISPGEA RLTNLEYMLE
410 420 430 440 450
SEDRGVQRDL TRVPLPTQLY NWAAPEVILQ KAATVKSDIY SFSMIMQEIL
460 470 480 490 500
TDDIPWKGLD GSVVKKAVVS GNYLEADVRL PKPYYDIVKS GIHVKQKDRT
510 520 530 540 550
MNLQDIRYIL KNDLKDFTGA QRTQPTESPR VQRYGLHPDV NVYLGLTSEH
560 570 580 590 600
PRETPDMEII ELKEMGSQPH SPRVHSLFTE GTLDPQAPDP CLMARETQNQ
610 620 630 640 650
DAPCPAPFMA EEASSPSTGQ PSLCSFEINE IYSGCLILED DIEEPPGAAS
660 670 680 690 700
SLEADGPNQV DELKSMEEEL DKMEREACCF GSEDESSSKA ETEYSFDDWD
710 720 730 740 750
WQNGSLSSLS LPESTREAKS NLNNMSTTEE YLISKCVLDL KIMQTIMHEN
760 770 780 790 800
DDRLRNIEQI LDEVEMKQKE QEERMSLWAT SREFTNAYKL PLAVGPPSLN
810 820 830 840 850
YIPPVLQLSG GQKPDTSGNY PTLPRFPRML PTLCDPGKQN TDEQFQCTQG
860 870 880 890 900
AKDSLETSRI QNTSSQGRPR ESTAQAKATQ FNSALFTLSS HRQGPSASPS
910 920 930 940 950
CHWDSTRMSV EPVSSEIYNA ESRNKDDGKV HLKWKMEVKE MAKKAATGQL
960 970 980 990 1000
TVPPWHPQSS LTLESEAENE PDALLQPPIR SPENTDWQRV IEYHRENDEP
1010 1020 1030 1040 1050
RGNGKFDKTG NNDCDSDQHG RQPRLGSFTS IRHPSPRQKE QPEHSEAFQA
1060 1070 1080 1090 1100
SSDTLVAVEK SYSHQSMQST CSPESSEDIT DEFLTPDGEY FYSSTAQENL
1110 1120 1130 1140 1150
ALETSSPIEE DFEGIQGAFA QPQVSGEEKF QMRKILGKNA EILPRSQFQP
1160 1170 1180 1190 1200
VRSTEDEQEE TSKESPKELK EKDISLTDIQ DLSSISYEPD SSFKEASCKT
1210 1220 1230 1240 1250
PKINHAPTSV STPLSPGSVS SAASQYKDCL ESITFQVKTE FASCWNSQEF
1260 1270 1280 1290 1300
IQTLSDDFIS VRERAKKLDS LLTSSETPPS RLTGLKRLSS FIGAGSPSLV
1310 1320 1330 1340 1350
KACDSSPPHA TQRRSLPKVE AFSQHHIDEL PPPSQELLDD IELLKQQQGS
1360 1370 1380 1390 1400
STVLHENTAS DGGGTANDQR HLEEQETDSK KEDSSMLLSK ETEDLGEDTE
1410 1420 1430 1440 1450
RAHSTLDEDL ERWLQPPEES VELQDLPKGS ERETNIKDQK VGEEKRKRED
1460 1470 1480 1490
SITPERRKSE GVLGTSEEDE LKSCFWKRLG WSESSRIIVL DQSDLSD
Length:1,497
Mass (Da):167,901
Last modified:July 25, 2006 - v2
Checksum:iD7DC446A1A18FC4D
GO
Isoform 2 (identifier: Q8IWB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-218: Missing.

Show »
Length:1,491
Mass (Da):167,140
Checksum:iAC936A9224CC0DA9
GO
Isoform 3 (identifier: Q8IWB6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-218: Missing.
     1064-1103: Missing.

Show »
Length:1,451
Mass (Da):162,669
Checksum:i10F8DE7EF828BC3E
GO

Sequence cautioni

The sequence AAK31980 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1326H → R in AAK31980 (PubMed:11279525).Curated1
Sequence conflicti1387L → P in CAD38856 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04138888D → G.1 PublicationCorresponds to variant rs56292204dbSNPEnsembl.1
Natural variantiVAR_041389321K → E in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041390443S → C.1 Publication1
Natural variantiVAR_041391559I → M.1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019868213 – 218Missing in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_0198691064 – 1103Missing in isoform 3. 2 PublicationsAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL834143 mRNA. Translation: CAD38856.2.
AC005666 Genomic DNA. No translation available.
BC040526 mRNA. Translation: AAH40526.1.
AF285601 mRNA. Translation: AAK31980.1. Different initiation.
BK000998 mRNA. Translation: DAA01359.1.
CCDSiCCDS32692.1. [Q8IWB6-3]
CCDS32693.1. [Q8IWB6-2]
CCDS56042.1. [Q8IWB6-1]
RefSeqiNP_001188386.1. NM_001201457.1. [Q8IWB6-1]
NP_112562.3. NM_031272.4. [Q8IWB6-3]
NP_938207.2. NM_198393.3. [Q8IWB6-2]
UniGeneiHs.390221.

Genome annotation databases

EnsembliENST00000240361; ENSP00000240361; ENSG00000121101. [Q8IWB6-1]
ENST00000349033; ENSP00000268910; ENSG00000121101. [Q8IWB6-3]
ENST00000389934; ENSP00000374584; ENSG00000121101. [Q8IWB6-2]
GeneIDi56155.
KEGGihsa:56155.
UCSCiuc002iwr.3. human. [Q8IWB6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL834143 mRNA. Translation: CAD38856.2.
AC005666 Genomic DNA. No translation available.
BC040526 mRNA. Translation: AAH40526.1.
AF285601 mRNA. Translation: AAK31980.1. Different initiation.
BK000998 mRNA. Translation: DAA01359.1.
CCDSiCCDS32692.1. [Q8IWB6-3]
CCDS32693.1. [Q8IWB6-2]
CCDS56042.1. [Q8IWB6-1]
RefSeqiNP_001188386.1. NM_001201457.1. [Q8IWB6-1]
NP_112562.3. NM_031272.4. [Q8IWB6-3]
NP_938207.2. NM_198393.3. [Q8IWB6-2]
UniGeneiHs.390221.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WUTX-ray2.30C/F/I/L792-804[»]
3WUUX-ray2.90C/F/I/L791-805[»]
ProteinModelPortaliQ8IWB6.
SMRiQ8IWB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121089. 3 interactors.
IntActiQ8IWB6. 3 interactors.
STRINGi9606.ENSP00000240361.

Protein family/group databases

TCDBi1.A.87.2.7. the mechanosensitive calcium channel (mca) family.

PTM databases

iPTMnetiQ8IWB6.
PhosphoSitePlusiQ8IWB6.

Polymorphism and mutation databases

BioMutaiTEX14.
DMDMi110816441.

Proteomic databases

PaxDbiQ8IWB6.
PeptideAtlasiQ8IWB6.
PRIDEiQ8IWB6.

Protocols and materials databases

DNASUi56155.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240361; ENSP00000240361; ENSG00000121101. [Q8IWB6-1]
ENST00000349033; ENSP00000268910; ENSG00000121101. [Q8IWB6-3]
ENST00000389934; ENSP00000374584; ENSG00000121101. [Q8IWB6-2]
GeneIDi56155.
KEGGihsa:56155.
UCSCiuc002iwr.3. human. [Q8IWB6-1]

Organism-specific databases

CTDi56155.
DisGeNETi56155.
GeneCardsiTEX14.
HGNCiHGNC:11737. TEX14.
HPAiHPA030620.
MIMi605792. gene.
neXtProtiNX_Q8IWB6.
OpenTargetsiENSG00000121101.
PharmGKBiPA36454.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHZA. Eukaryota.
ENOG410XT67. LUCA.
GeneTreeiENSGT00390000015123.
HOGENOMiHOG000059264.
HOVERGENiHBG094035.
InParanoidiQ8IWB6.
KOiK17540.
OMAiLEYMMES.
OrthoDBiEOG091G0CDS.
PhylomeDBiQ8IWB6.
TreeFamiTF328704.

Miscellaneous databases

GenomeRNAii56155.
PROiQ8IWB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000121101.
CleanExiHS_TEX14.
ExpressionAtlasiQ8IWB6. baseline and differential.
GenevisibleiQ8IWB6. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTEX14_HUMAN
AccessioniPrimary (citable) accession number: Q8IWB6
Secondary accession number(s): A6NH19
, Q7RTP3, Q8ND97, Q9BXT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Ser-376 is present instead of the conserved Asp which is expected to be an active site residue.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.