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Q8IW75 (SPA12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serpin A12
Alternative name(s):
OL-64
Visceral adipose tissue-derived serine protease inhibitor
Short name=Vaspin
Visceral adipose-specific serpin
Gene names
Name:SERPINA12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues. Ref.1

Subunit structure

Forms a stable complex with KLK7. Ref.4

Subcellular location

Secreted.

Tissue specificity

Expressed in visceral adipose tissues. Ref.1

Domain

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable By similarity.

Sequence similarities

Belongs to the serpin family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 414394Serpin A12
PRO_0000041976

Regions

Region364 – 38219RCL

Amino acid modifications

Glycosylation2211N-linked (GlcNAc...) Potential
Glycosylation2331N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential

Natural variations

Natural variant1421Q → K.
Corresponds to variant rs17090972 [ dbSNP | Ensembl ].
VAR_051943
Natural variant3941I → V.
Corresponds to variant rs34519784 [ dbSNP | Ensembl ].
VAR_051944

Experimental info

Mutagenesis3651T → R: Fails to inhibit KLK7 activity. Ref.4
Mutagenesis3691A → P: Fails to inhibit KLK7 activity. Ref.4

Secondary structure

....................................................... 414
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8IW75 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5C70F1AB5935661C

FASTA41447,175
        10         20         30         40         50         60 
MNPTLGLAIF LAVLLTVKGL LKPSFSPRNY KALSEVQGWK QRMAAKELAR QNMDLGFKLL 

        70         80         90        100        110        120 
KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ GFNFRKMPEK DLHEGFHYII 

       130        140        150        160        170        180 
HELTQKTQDL KLSIGNTLFI DQRLQPQRKF LEDAKNFYSA ETILTNFQNL EMAQKQINDF 

       190        200        210        220        230        240 
ISQKTHGKIN NLIENIDPGT VMLLANYIFF RARWKHEFDP NVTKEEDFFL EKNSSVKVPM 

       250        260        270        280        290        300 
MFRSGIYQVG YDDKLSCTIL EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS 

       310        320        330        340        350        360 
RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK IAPHRSLKVG EAVHKAELKM 

       370        380        390        400        410 
DERGTEGAAG TGAQTLPMET PLVVKIDKPY LLLIYSEKIP SVLFLGKIVN PIGK 

« Hide

References

« Hide 'large scale' references
[1]"Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity."
Hida K., Wada J., Eguchi J., Zhang H., Baba M., Seida A., Hashimoto I., Okada T., Yasuhara A., Nakatsuka A., Shikata K., Hourai S., Futami J., Watanabe E., Matsuki Y., Hiramatsu R., Akagi S., Makino H., Kanwar Y.S.
Proc. Natl. Acad. Sci. U.S.A. 102:10610-10615(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]Chen S., Guo J.H., Yu L.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[4]"Vaspin inhibits kallikrein 7 by serpin mechanism."
Heiker J.T., Kloting N., Kovacs P., Kuettner E.B., Strater N., Schultz S., Kern M., Stumvoll M., Bluher M., Beck-Sickinger A.G.
Cell. Mol. Life Sci. 70:2569-2583(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 22-414, MUTAGENESIS OF THR-365 AND ALA-369, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY326420 mRNA. Translation: AAP88384.1.
AY177692 mRNA. Translation: AAO18649.1.
BC040857 mRNA. Translation: AAH40857.1.
CCDSCCDS9926.1.
RefSeqNP_776249.1. NM_173850.2.
XP_005267408.1. XM_005267351.1.
UniGeneHs.99476.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4IF8X-ray2.08A/B22-414[»]
ProteinModelPortalQ8IW75.
SMRQ8IW75. Positions 37-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126901. 2 interactions.
IntActQ8IW75. 1 interaction.
STRING9606.ENSP00000342109.

Protein family/group databases

MEROPSI04.091.

PTM databases

PhosphoSiteQ8IW75.

Polymorphism databases

DMDM74728144.

Proteomic databases

PaxDbQ8IW75.
PRIDEQ8IW75.

Protocols and materials databases

DNASU145264.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000341228; ENSP00000342109; ENSG00000165953.
ENST00000556881; ENSP00000451738; ENSG00000165953.
GeneID145264.
KEGGhsa:145264.
UCSCuc001ydj.3. human.

Organism-specific databases

CTD145264.
GeneCardsGC14M094954.
HGNCHGNC:18359. SERPINA12.
neXtProtNX_Q8IW75.
PharmGKBPA134863157.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238521.
HOVERGENHBG005957.
InParanoidQ8IW75.
OMAIFFRARW.
OrthoDBEOG7QC7W9.
PhylomeDBQ8IW75.
TreeFamTF343201.

Gene expression databases

BgeeQ8IW75.
CleanExHS_SERPINA12.
GenevestigatorQ8IW75.

Family and domain databases

InterProIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi145264.
NextBio85065.
PROQ8IW75.

Entry information

Entry nameSPA12_HUMAN
AccessionPrimary (citable) accession number: Q8IW75
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM