Reviewed, UniProtKB/Swiss-Prot Q8IW35 (CEP97_HUMAN)

Names and origin

Protein names

Recommended name:
    Centrosomal protein of 97 kDa
      Short name=Cep97
Alternative name(s):
    Leucine-rich repeat and IQ domain-containing protein 2

Gene names

Name:	CEP97
Synonyms:	LRRIQ2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Collaborates with CEP110, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in cytokinesis. Required for recruitment of CEP110 to the centrosome. Ref.3
Subunit structure	Interacts with CALM1, CEP76 and CEP110.
Subcellular location	Centrosome. Ref.3
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5
Sequence similarities	Contains 1 IQ domain. Contains 6 LRR (leucine-rich) repeats.
Sequence caution	The sequence BAC04055.1 differs from that shown. Reason: Frameshift at position 303.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CEP110	O43303	5	EBI-1566210,EBI-1566217

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IW35-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IW35-2)

The sequence of this isoform differs from the canonical sequence as follows:
     631-631: Q → QKWGLAILPRPVSNFWAQAVFPPQPPK

Note: No experimental confirmation available. Sequence incomplete.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	Centrosomal protein of 97 kDa		PRO_0000263705
Regions
	Repeat	35 – 59	25	LRR 1
	Repeat	79 – 101	23	LRR 2
	Repeat	102 – 123	22	LRR 3
	Repeat	124 – 147	24	LRR 4
	Repeat	169 – 192	24	LRR 5
	Repeat	194 – 219	26	LRR 6
	Domain	558 – 587	30	IQ
	Region	300 – 750	451	CEP110 binding
Amino acid modifications
	Modified residue	542	1	Phosphothreonine Ref.4
	Modified residue	763	1	Phosphoserine Ref.5
	Modified residue	770	1	Phosphoserine Ref.5
	Modified residue	811	1	Phosphothreonine Ref.5
	Modified residue	813	1	Phosphoserine Ref.5
	Modified residue	817	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	631	1	Q → QKWGLAILPRPVSNFWAQAV FPPQPPK in isoform 2.		VSP_031155
Experimental info
	Sequence conflict	299 – 300	2	Missing in BAC04055. Ref.2
	Sequence conflict	399	1	L → F in BAC04055. Ref.2
	Sequence conflict	758	1	Missing in BAB15531. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 98B1C230DF586DF7	FASTA	865	96,981
	10 20 30 40 50 60 MAVARVDAAL PPGEGSVVNW SGQGLQKLGP NLPCEADIHT LILDKNQIIK LENLEKCKRL 70 80 90 100 110 120 IQLSVANNRL VRMMGVAKLT LLRVLNLPHN SIGCVEGLKE LVHLEWLNLA GNNLKAMEQI 130 140 150 160 170 180 NSCTALQHLD LSDNNISQIG DLSKLVSLKT LLLHGNIITS LRMAPAYLPR SLAILSLAEN 190 200 210 220 230 240 EIRDLNEISF LASLTELEQL SIMNNPCVMA TPSIPGFDYR PYIVSWCLNL RVLDGYVISQ 250 260 270 280 290 300 KESLKAEWLY SQGKGRAYRP GQHIQLVQYL ATVCPLTSTL GLQTAEDAKL EKILSKQRFH 310 320 330 340 350 360 QRQLMNQSQN EELSPLVPVE TRASLIPEHS SPVQDCQISQ ESEPVIQVNS WVGINSNDDQ 370 380 390 400 410 420 LFAVKNNFPA SVHTTRYSRN DLHLEDIQTD EDKLNCSLLS SESTFMPVAS GLSPLSPTVE 430 440 450 460 470 480 LRLQGINLGL EDDGVADESV KGLESQVLDK EEEQPLWAAN ENSVQMMRSE INTEVNEKAG 490 500 510 520 530 540 LLPCPEPTII SAILKDDNHS LTFFPESTEQ KQSDIKKPEN TQPENKETIS QATSEKLPMI 550 560 570 580 590 600 LTQRSVALGQ DKVALQKLND AATKLQACWR GFYARNYNPQ AKDVRYEIRL RRMQEHIVCL 610 620 630 640 650 660 TDEIRRLRKE RDEERIKKFV QEEAFRFLWN QVRSLQVWQQ TVDQRLSSWH TDVPPISSTL 670 680 690 700 710 720 VPSKHPLFTQ SQESSCDQNA DWFIASDVAP QEKSLPEFPD SGFHSSLTEQ VHSLQHSLDF 730 740 750 760 770 780 EKSSTEGSES SIMGNSIDTV RYGKESDLGD VSEEHGEWNK ESSNNEQDNS LLEQYLTSVQ 790 800 810 820 830 840 QLEDADERTN FDTETRDSKL HIACFPVQLD TLSDGASVDE SHGISPPLQG EISQTQENSK 850 860 LNAEVQGQQP ECDSTFQLLH VGVTV
	Isoform 2. Checksum: 7A3DA414E33B7E6C	FASTA	891	99,909
	10 20 30 40 50 60 MAVARVDAAL PPGEGSVVNW SGQGLQKLGP NLPCEADIHT LILDKNQIIK LENLEKCKRL 70 80 90 100 110 120 IQLSVANNRL VRMMGVAKLT LLRVLNLPHN SIGCVEGLKE LVHLEWLNLA GNNLKAMEQI 130 140 150 160 170 180 NSCTALQHLD LSDNNISQIG DLSKLVSLKT LLLHGNIITS LRMAPAYLPR SLAILSLAEN 190 200 210 220 230 240 EIRDLNEISF LASLTELEQL SIMNNPCVMA TPSIPGFDYR PYIVSWCLNL RVLDGYVISQ 250 260 270 280 290 300 KESLKAEWLY SQGKGRAYRP GQHIQLVQYL ATVCPLTSTL GLQTAEDAKL EKILSKQRFH 310 320 330 340 350 360 QRQLMNQSQN EELSPLVPVE TRASLIPEHS SPVQDCQISQ ESEPVIQVNS WVGINSNDDQ 370 380 390 400 410 420 LFAVKNNFPA SVHTTRYSRN DLHLEDIQTD EDKLNCSLLS SESTFMPVAS GLSPLSPTVE 430 440 450 460 470 480 LRLQGINLGL EDDGVADESV KGLESQVLDK EEEQPLWAAN ENSVQMMRSE INTEVNEKAG 490 500 510 520 530 540 LLPCPEPTII SAILKDDNHS LTFFPESTEQ KQSDIKKPEN TQPENKETIS QATSEKLPMI 550 560 570 580 590 600 LTQRSVALGQ DKVALQKLND AATKLQACWR GFYARNYNPQ AKDVRYEIRL RRMQEHIVCL 610 620 630 640 650 660 TDEIRRLRKE RDEERIKKFV QEEAFRFLWN QKWGLAILPR PVSNFWAQAV FPPQPPKVRS 670 680 690 700 710 720 LQVWQQTVDQ RLSSWHTDVP PISSTLVPSK HPLFTQSQES SCDQNADWFI ASDVAPQEKS 730 740 750 760 770 780 LPEFPDSGFH SSLTEQVHSL QHSLDFEKSS TEGSESSIMG NSIDTVRYGK ESDLGDVSEE 790 800 810 820 830 840 HGEWNKESSN NEQDNSLLEQ YLTSVQQLED ADERTNFDTE TRDSKLHIAC FPVQLDTLSD 850 860 870 880 890 GASVDESHGI SPPLQGEISQ TQENSKLNAE VQGQQPECDS TFQLLHVGVT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 161-696 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 714-865 (ISOFORM 1). Tissue: Lung and Testis.
[3]	"Cep97 and CP110 suppress a cilia assembly program." Spektor A., Tsang W.Y., Khoo D., Dynlacht B.D. Cell 130:678-690(2007) [PubMed: 17719545] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH CALM1 AND CEP110, SUBCELLULAR LOCATION.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-542, MASS SPECTROMETRY.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763; SER-770; THR-811; SER-813 AND SER-817, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Cep76, a centrosomal protein that specifically restrains centriole reduplication." Tsang W.Y., Spektor A., Vijayakumar S., Bista B.R., Li J., Sanchez I., Duensing S., Dynlacht B.D. Dev. Cell 16:649-660(2009) [PubMed: 19460342] [Abstract] Cited for: INTERACTION WITH CEP76.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC041085 mRNA. Translation: AAH41085.1. AK026700 mRNA. Translation: BAB15531.1. Different initiation. AK093100 mRNA. Translation: BAC04055.1. Frameshift.
IPI	IPI00238469. IPI00383439.
RefSeq	NP_078824.2.
UniGene	Hs.444135
3D structure databases
HSSP	HSSP built from PDB template 1D0B based on UniProtKB P25147.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IW35. 2 interactions.
PTM databases
PhosphoSite	Q8IW35.
Proteomic databases
PRIDE	Q8IW35.
Genome annotation databases
Ensembl	ENSG00000182504. Homo sapiens. [Contig view]
GeneID	79598.
KEGG	hsa:79598.
UCSC	uc003dvk.1. human.
Organism-specific databases
GeneCards	GC03P102927.
HGNC	HGNC:26244. CEP97.
PharmGKB	PA134910269.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8IW35.
OMA	Q8IW35. ACWRGFY.
Gene expression databases
ArrayExpress	Q8IW35.
Bgee	Q8IW35.
CleanEx	HS_CEP97.
GermOnline	ENSG00000182504. Homo sapiens.
Family and domain databases
InterPro	IPR000048. IQ_CaM_bd_region. IPR001611. Leu-rich_rpt. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF00560. LRR_1. 1 hit. [Graphical view]
SMART	SM00015. IQ. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	68622.

Entry information

Entry name

CEP97_HUMAN

Accession

Primary (citable) accession number: Q8IW35
Secondary accession number(s): Q8NA71, Q9H5T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 1, 2003
Last modified:	July 7, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)