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Protein

Centrosomal protein of 97 kDa

Gene

CEP97

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.1 Publication

GO - Biological processi

  • cell projection organization Source: UniProtKB-KW
  • negative regulation of cilium assembly Source: UniProtKB
  • regulation of mitotic spindle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-HSA-5620912. Anchoring of the basal body to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 97 kDa
Short name:
Cep97
Alternative name(s):
Leucine-rich repeat and IQ domain-containing protein 2
Gene namesi
Name:CEP97
Synonyms:LRRIQ2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:26244. CEP97.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • microtubule organizing center Source: HPA
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382176.

Polymorphism and mutation databases

BioMutaiCEP97.
DMDMi74762481.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865Centrosomal protein of 97 kDaPRO_0000263705Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081PhosphoserineCombined sources
Modified residuei413 – 4131PhosphoserineCombined sources
Modified residuei500 – 5001PhosphoserineCombined sources
Modified residuei530 – 5301PhosphoserineBy similarity
Modified residuei542 – 5421PhosphothreonineCombined sources
Modified residuei763 – 7631PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IW35.
MaxQBiQ8IW35.
PaxDbiQ8IW35.
PeptideAtlasiQ8IW35.
PRIDEiQ8IW35.

PTM databases

iPTMnetiQ8IW35.
PhosphoSiteiQ8IW35.

Expressioni

Gene expression databases

BgeeiENSG00000182504.
CleanExiHS_CEP97.
ExpressionAtlasiQ8IW35. baseline and differential.
GenevisibleiQ8IW35. HS.

Organism-specific databases

HPAiHPA002980.

Interactioni

Subunit structurei

Interacts with CALM1, CEP76, CCP110, KIF24, TALPID3. Via its interaction with CCP110, may indirectly interact with HERC2 and NEURL4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCP110O4330323EBI-1566210,EBI-1566217
KIF24Q5T7B810EBI-1566210,EBI-2556811

Protein-protein interaction databases

BioGridi122737. 57 interactions.
IntActiQ8IW35. 47 interactions.
MINTiMINT-8392027.
STRINGi9606.ENSP00000342510.

Structurei

3D structure databases

ProteinModelPortaliQ8IW35.
SMRiQ8IW35. Positions 21-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 5822LRR 1Add
BLAST
Repeati59 – 8022LRR 2Add
BLAST
Repeati81 – 10222LRR 3Add
BLAST
Repeati103 – 12422LRR 4Add
BLAST
Repeati125 – 14622LRR 5Add
BLAST
Repeati147 – 16822LRR 6Add
BLAST
Repeati171 – 19222LRR 7Add
BLAST
Repeati196 – 20510LRR 8
Domaini211 – 24939LRRCTAdd
BLAST
Domaini558 – 58730IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 750451CCP110-bindingAdd
BLAST

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 8 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0531. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00830000128399.
HOGENOMiHOG000111550.
HOVERGENiHBG061575.
InParanoidiQ8IW35.
KOiK16717.
OMAiKEVRYEI.
OrthoDBiEOG091G02EC.
PhylomeDBiQ8IW35.
TreeFamiTF320816.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IW35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVARVDAAL PPGEGSVVNW SGQGLQKLGP NLPCEADIHT LILDKNQIIK
60 70 80 90 100
LENLEKCKRL IQLSVANNRL VRMMGVAKLT LLRVLNLPHN SIGCVEGLKE
110 120 130 140 150
LVHLEWLNLA GNNLKAMEQI NSCTALQHLD LSDNNISQIG DLSKLVSLKT
160 170 180 190 200
LLLHGNIITS LRMAPAYLPR SLAILSLAEN EIRDLNEISF LASLTELEQL
210 220 230 240 250
SIMNNPCVMA TPSIPGFDYR PYIVSWCLNL RVLDGYVISQ KESLKAEWLY
260 270 280 290 300
SQGKGRAYRP GQHIQLVQYL ATVCPLTSTL GLQTAEDAKL EKILSKQRFH
310 320 330 340 350
QRQLMNQSQN EELSPLVPVE TRASLIPEHS SPVQDCQISQ ESEPVIQVNS
360 370 380 390 400
WVGINSNDDQ LFAVKNNFPA SVHTTRYSRN DLHLEDIQTD EDKLNCSLLS
410 420 430 440 450
SESTFMPVAS GLSPLSPTVE LRLQGINLGL EDDGVADESV KGLESQVLDK
460 470 480 490 500
EEEQPLWAAN ENSVQMMRSE INTEVNEKAG LLPCPEPTII SAILKDDNHS
510 520 530 540 550
LTFFPESTEQ KQSDIKKPEN TQPENKETIS QATSEKLPMI LTQRSVALGQ
560 570 580 590 600
DKVALQKLND AATKLQACWR GFYARNYNPQ AKDVRYEIRL RRMQEHIVCL
610 620 630 640 650
TDEIRRLRKE RDEERIKKFV QEEAFRFLWN QVRSLQVWQQ TVDQRLSSWH
660 670 680 690 700
TDVPPISSTL VPSKHPLFTQ SQESSCDQNA DWFIASDVAP QEKSLPEFPD
710 720 730 740 750
SGFHSSLTEQ VHSLQHSLDF EKSSTEGSES SIMGNSIDTV RYGKESDLGD
760 770 780 790 800
VSEEHGEWNK ESSNNEQDNS LLEQYLTSVQ QLEDADERTN FDTETRDSKL
810 820 830 840 850
HIACFPVQLD TLSDGASVDE SHGISPPLQG EISQTQENSK LNAEVQGQQP
860
ECDSTFQLLH VGVTV
Length:865
Mass (Da):96,981
Last modified:March 1, 2003 - v1
Checksum:i98B1C230DF586DF7
GO
Isoform 2 (identifier: Q8IW35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     631-631: Q → QKWGLAILPRPVSNFWAQAVFPPQPPK

Note: No experimental confirmation available. Sequence incomplete.
Show »
Length:891
Mass (Da):99,909
Checksum:i7A3DA414E33B7E6C
GO

Sequence cautioni

The sequence BAB15531 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC04055 differs from that shown. Reason: Frameshift at position 303. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 3002Missing in BAC04055 (PubMed:14702039).Curated
Sequence conflicti399 – 3991L → F in BAC04055 (PubMed:14702039).Curated
Sequence conflicti758 – 7581Missing in BAB15531 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei631 – 6311Q → QKWGLAILPRPVSNFWAQAV FPPQPPK in isoform 2. 1 PublicationVSP_031155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084198 Genomic DNA. No translation available.
BC041085 mRNA. Translation: AAH41085.1.
AK026700 mRNA. Translation: BAB15531.1. Different initiation.
AK093100 mRNA. Translation: BAC04055.1. Frameshift.
CCDSiCCDS2944.1. [Q8IW35-1]
RefSeqiNP_001290330.1. NM_001303401.1.
NP_078824.2. NM_024548.3. [Q8IW35-1]
UniGeneiHs.444135.

Genome annotation databases

EnsembliENST00000341893; ENSP00000342510; ENSG00000182504. [Q8IW35-1]
GeneIDi79598.
KEGGihsa:79598.
UCSCiuc003dvk.2. human. [Q8IW35-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084198 Genomic DNA. No translation available.
BC041085 mRNA. Translation: AAH41085.1.
AK026700 mRNA. Translation: BAB15531.1. Different initiation.
AK093100 mRNA. Translation: BAC04055.1. Frameshift.
CCDSiCCDS2944.1. [Q8IW35-1]
RefSeqiNP_001290330.1. NM_001303401.1.
NP_078824.2. NM_024548.3. [Q8IW35-1]
UniGeneiHs.444135.

3D structure databases

ProteinModelPortaliQ8IW35.
SMRiQ8IW35. Positions 21-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122737. 57 interactions.
IntActiQ8IW35. 47 interactions.
MINTiMINT-8392027.
STRINGi9606.ENSP00000342510.

PTM databases

iPTMnetiQ8IW35.
PhosphoSiteiQ8IW35.

Polymorphism and mutation databases

BioMutaiCEP97.
DMDMi74762481.

Proteomic databases

EPDiQ8IW35.
MaxQBiQ8IW35.
PaxDbiQ8IW35.
PeptideAtlasiQ8IW35.
PRIDEiQ8IW35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341893; ENSP00000342510; ENSG00000182504. [Q8IW35-1]
GeneIDi79598.
KEGGihsa:79598.
UCSCiuc003dvk.2. human. [Q8IW35-1]

Organism-specific databases

CTDi79598.
GeneCardsiCEP97.
HGNCiHGNC:26244. CEP97.
HPAiHPA002980.
MIMi615864. gene.
neXtProtiNX_Q8IW35.
PharmGKBiPA162382176.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0531. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00830000128399.
HOGENOMiHOG000111550.
HOVERGENiHBG061575.
InParanoidiQ8IW35.
KOiK16717.
OMAiKEVRYEI.
OrthoDBiEOG091G02EC.
PhylomeDBiQ8IW35.
TreeFamiTF320816.

Enzyme and pathway databases

ReactomeiR-HSA-5620912. Anchoring of the basal body to the plasma membrane.

Miscellaneous databases

GeneWikiiCEP97.
GenomeRNAii79598.
PROiQ8IW35.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182504.
CleanExiHS_CEP97.
ExpressionAtlasiQ8IW35. baseline and differential.
GenevisibleiQ8IW35. HS.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEP97_HUMAN
AccessioniPrimary (citable) accession number: Q8IW35
Secondary accession number(s): B5MDY8, Q8NA71, Q9H5T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.