Q8IVW6 (ARI3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AT-rich interactive domain-containing protein 3B
Short name=ARID domain-containing protein 3B

Alternative name(s):
Bright and dead ringer protein
Bright-like protein

Gene names

Name:	ARID3B
Synonyms:	BDP, DRIL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A. Ref.5 Ref.6
Subunit structure	Heterodimer with ARID3A. Interacts with unphosphorylated RB1. Ref.1 Ref.6
Subcellular location	Nucleus Ref.1 Ref.6.
Tissue specificity	Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level). Ref.1 Ref.5
Sequence similarities	Contains 1 ARID domain. Contains 1 REKLES domain.
Sequence caution	The sequence BAD92067.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Tumor suppressor
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	DNA binding Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IVW6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3 (identifier: Q8IVW6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     390-471: DGAPVTTVPV...RQLPMKIRIN → QQGSWGRRKK...PVPLQVMEPQ
     472-561: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8IVW6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     474-474: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	AT-rich interactive domain-containing protein 3B		PRO_0000295162
Regions
	Domain	215 – 307	93	ARID
	Domain	419 – 517	99	REKLES
	Region	203 – 365	163	Interaction with RB1
	Region	490 – 513	24	Interaction with ARID3A
	Compositional bias	5 – 49	45	Gln-rich
	Compositional bias	341 – 353	13	Poly-Ala
	Compositional bias	523 – 560	38	Ser-rich
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.8
	Modified residue	89	1	Phosphoserine Ref.7 Ref.9
	Modified residue	165	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	390 – 471	82	DGAPV…KIRIN → QQGSWGRRKKGRKGCQFPVF KTGVLGQEGQLWIGPAPVPL QVMEPQ in isoform 3.		VSP_026773
	Alternative sequence	472 – 561	90	Missing in isoform 3.		VSP_026774
	Alternative sequence	474	1	Missing in isoform 4.		VSP_039860
Experimental info
	Mutagenesis	240	1	P → H: Impairs binding to RB1. Ref.1
	Mutagenesis	271	1	W → S: Impairs binding to RB1. Ref.1
	Sequence conflict	7	1	Q → QQ in AAH41792. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 5F0CFF83BAFC12D2	FASTA	561	60,637
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 250 260 270 280 290 300 IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 310 320 330 340 350 360 AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 370 380 390 400 410 420 RFPILGLGSS SGTNTSSPRI SPATTLRKGD GAPVTTVPVP NRLAVPVTLA SQQAGTRTAA 430 440 450 460 470 480 LEQLRERLES GEPAEKKASR LSEEEQRLVQ QAFQRNFFSM ARQLPMKIRI NGRAEDRAEA 490 500 510 520 530 540 SAAALNLTTS SIGSINMSVD IDGTTYAGVL FAQKPVVHLI TGSAPQSLGS SASSSSSSHC 550 560 SPSPTSSRGT PSAEPSTSWS L
	Isoform 3 [UniParc]. Checksum: 10364C23137AFDB9	FASTA	435	47,661
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 250 260 270 280 290 300 IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 310 320 330 340 350 360 AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 370 380 390 400 410 420 RFPILGLGSS SGTNTSSPRI SPATTLRKGQ QGSWGRRKKG RKGCQFPVFK TGVLGQEGQL 430 WIGPAPVPLQ VMEPQ
	Isoform 4 [UniParc]. Checksum: 93A741BF1B631B6A	FASTA	560	60,566
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 250 260 270 280 290 300 IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 310 320 330 340 350 360 AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 370 380 390 400 410 420 RFPILGLGSS SGTNTSSPRI SPATTLRKGD GAPVTTVPVP NRLAVPVTLA SQQAGTRTAA 430 440 450 460 470 480 LEQLRERLES GEPAEKKASR LSEEEQRLVQ QAFQRNFFSM ARQLPMKIRI NGREDRAEAS 490 500 510 520 530 540 AAALNLTTSS IGSINMSVDI DGTTYAGVLF AQKPVVHLIT GSAPQSLGSS ASSSSSSHCS 550 560 PSPTSSRGTP SAEPSTSWSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bdp, a new member of a family of DNA-binding proteins, associates with the retinoblastoma gene product." Numata S., Claudio P.P., Dean C., Giordano A., Croce C.M. Cancer Res. 59:3741-3747(1999) [PubMed: 10446990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH RB1, MUTAGENESIS OF PRO-240 AND TRP-271. Tissue: Testis.
[2]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[5]	"ARID3B induces malignant transformation of mouse embryonic fibroblasts and is strongly associated with malignant neuroblastoma." Kobayashi K., Era T., Takebe A., Jakt L.M., Nishikawa S. Cancer Res. 66:8331-8336(2006) [PubMed: 16951138] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"REKLES is an ARID3-restricted multifunctional domain." Kim D., Probst L., Das C., Tucker P.W. J. Biol. Chem. 282:15768-15777(2007) [PubMed: 17400556] [Abstract] Cited for: INTERACTION WITH ARID3A, SUBCELLULAR LOCATION, FUNCTION.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF116846 mRNA. Translation: AAD09133.1. AB208830 mRNA. Translation: BAD92067.1. Different initiation. AC100835 Genomic DNA. No translation available. BC041792 mRNA. Translation: AAH41792.1.
IPI	IPI00328266. IPI00793438. IPI00853367.
RefSeq	NP_006456.1. NM_006465.2.
UniGene	Hs.655532.
3D structure databases
HSSP	HSSP built from PDB template 1KQQ based on UniProtKB Q24573.
ProteinModelPortal	Q8IVW6.
SMR	Q8IVW6. Positions 176-328.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8IVW6.
PTM databases
PhosphoSite	Q8IVW6.
Polymorphism databases
DMDM	152013359.
Proteomic databases
PRIDE	Q8IVW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000395077; ENSP00000378515; ENSG00000179361.
GeneID	10620.
KEGG	hsa:10620.
UCSC	uc002ayc.2. human. uc002ayd.1. human.
Organism-specific databases
CTD	10620.
GeneCards	GC15P074833.
HGNC	HGNC:14350. ARID3B.
MIM	612457. gene.
neXtProt	NX_Q8IVW6.
PharmGKB	PA134896829.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19759.
GeneTree	ENSGT00550000074575.
HOGENOM	HBG402789.
HOVERGEN	HBG103680.
InParanoid	Q8IVW6.
OMA	FSMARQL.
Gene expression databases
ArrayExpress	Q8IVW6.
Bgee	Q8IVW6.
CleanEx	HS_ARID3B.
Genevestigator	Q8IVW6.
Family and domain databases
InterPro	IPR001606. ARID/BRIGHT_DNA-bd. IPR023334. REKLES_domain. [Graphical view]
Gene3D	G3DSA:1.10.150.60. ARID. 1 hit.
Pfam	PF01388. ARID. 1 hit. [Graphical view]
SMART	SM00501. BRIGHT. 1 hit. [Graphical view]
SUPFAM	SSF46774. ARID. 1 hit.
PROSITE	PS51011. ARID. 1 hit. PS51486. REKLES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	40352.
SOURCE	Search...

Entry information

Entry name

ARI3B_HUMAN

Accession

Primary (citable) accession number: Q8IVW6
Secondary accession number(s): O95443, Q59HC9, Q6P9C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.