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Q8IVW6 (ARI3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AT-rich interactive domain-containing protein 3B

Short name=ARID domain-containing protein 3B
Alternative name(s):
Bright and dead ringer protein
Bright-like protein
Gene names
Name:ARID3B
Synonyms:BDP, DRIL2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A. Ref.5 Ref.6

Subunit structure

Heterodimer with ARID3A. Interacts with unphosphorylated RB1. Ref.1 Ref.6

Subcellular location

Nucleus Ref.1 Ref.6.

Tissue specificity

Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K-562 erythrocytic leukemia cell line (at protein level). Ref.1 Ref.5

Sequence similarities

Contains 1 ARID domain.

Contains 1 REKLES domain.

Sequence caution

The sequence BAD92067.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   LigandDNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Non-traceable author statement Ref.1. Source: UniProtKB

   Molecular_functionDNA binding

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IVW6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 3 (identifier: Q8IVW6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     390-471: DGAPVTTVPV...RQLPMKIRIN → QQGSWGRRKK...PVPLQVMEPQ
     472-561: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8IVW6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     474-474: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561AT-rich interactive domain-containing protein 3B
PRO_0000295162

Regions

Domain215 – 30793ARID
Domain419 – 51799REKLES
Region203 – 365163Interaction with RB1
Region490 – 51324Interaction with ARID3A
Compositional bias5 – 4945Gln-rich
Compositional bias341 – 35313Poly-Ala
Compositional bias523 – 56038Ser-rich

Amino acid modifications

Modified residue11N-acetylmethionine Ref.8
Modified residue891Phosphoserine Ref.7 Ref.9 Ref.11

Natural variations

Alternative sequence390 – 47182DGAPV…KIRIN → QQGSWGRRKKGRKGCQFPVF KTGVLGQEGQLWIGPAPVPL QVMEPQ in isoform 3.
VSP_026773
Alternative sequence472 – 56190Missing in isoform 3.
VSP_026774
Alternative sequence4741Missing in isoform 4.
VSP_039860

Experimental info

Mutagenesis2401P → H: Impairs binding to RB1. Ref.1
Mutagenesis2711W → S: Impairs binding to RB1. Ref.1
Sequence conflict71Q → QQ in AAH41792. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 5F0CFF83BAFC12D2

FASTA56160,637
        10         20         30         40         50         60 
MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 

        70         80         90        100        110        120 
SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 

       130        140        150        160        170        180 
YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 

       190        200        210        220        230        240 
QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 

       250        260        270        280        290        300 
IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 

       310        320        330        340        350        360 
AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 

       370        380        390        400        410        420 
RFPILGLGSS SGTNTSSPRI SPATTLRKGD GAPVTTVPVP NRLAVPVTLA SQQAGTRTAA 

       430        440        450        460        470        480 
LEQLRERLES GEPAEKKASR LSEEEQRLVQ QAFQRNFFSM ARQLPMKIRI NGRAEDRAEA 

       490        500        510        520        530        540 
SAAALNLTTS SIGSINMSVD IDGTTYAGVL FAQKPVVHLI TGSAPQSLGS SASSSSSSHC 

       550        560 
SPSPTSSRGT PSAEPSTSWS L 

« Hide

Isoform 3 [UniParc].

Checksum: 10364C23137AFDB9
Show »

FASTA43547,661
Isoform 4 [UniParc].

Checksum: 93A741BF1B631B6A
Show »

FASTA56060,566

References

« Hide 'large scale' references
[1]"Bdp, a new member of a family of DNA-binding proteins, associates with the retinoblastoma gene product."
Numata S., Claudio P.P., Dean C., Giordano A., Croce C.M.
Cancer Res. 59:3741-3747(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH RB1, MUTAGENESIS OF PRO-240 AND TRP-271.
Tissue: Testis.
[2]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[3]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[5]"ARID3B induces malignant transformation of mouse embryonic fibroblasts and is strongly associated with malignant neuroblastoma."
Kobayashi K., Era T., Takebe A., Jakt L.M., Nishikawa S.
Cancer Res. 66:8331-8336(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"REKLES is an ARID3-restricted multifunctional domain."
Kim D., Probst L., Das C., Tucker P.W.
J. Biol. Chem. 282:15768-15777(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARID3A, SUBCELLULAR LOCATION, FUNCTION.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF116846 mRNA. Translation: AAD09133.1.
AB208830 mRNA. Translation: BAD92067.1. Different initiation.
AC100835 Genomic DNA. No translation available.
BC041792 mRNA. Translation: AAH41792.1.
CCDSCCDS10264.1. [Q8IVW6-4]
RefSeqNP_006456.1. NM_006465.2. [Q8IVW6-4]
XP_005254186.1. XM_005254129.1. [Q8IVW6-1]
UniGeneHs.655532.

3D structure databases

ProteinModelPortalQ8IVW6.
SMRQ8IVW6. Positions 176-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115865. 9 interactions.
STRING9606.ENSP00000343126.

PTM databases

PhosphoSiteQ8IVW6.

Polymorphism databases

DMDM152013359.

Proteomic databases

MaxQBQ8IVW6.
PaxDbQ8IVW6.
PRIDEQ8IVW6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000346246; ENSP00000343126; ENSG00000179361. [Q8IVW6-4]
GeneID10620.
KEGGhsa:10620.
UCSCuc002ayc.3. human. [Q8IVW6-3]
uc002ayd.3. human. [Q8IVW6-4]
uc002aye.3. human. [Q8IVW6-1]

Organism-specific databases

CTD10620.
GeneCardsGC15P074833.
HGNCHGNC:14350. ARID3B.
HPAHPA050320.
MIM612457. gene.
neXtProtNX_Q8IVW6.
PharmGKBPA134896829.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327790.
HOGENOMHOG000236281.
HOVERGENHBG103680.
InParanoidQ8IVW6.
OMAFHVQKVA.
OrthoDBEOG77DJ6W.
PhylomeDBQ8IVW6.
TreeFamTF320364.

Gene expression databases

ArrayExpressQ8IVW6.
BgeeQ8IVW6.
CleanExHS_ARID3B.
GenevestigatorQ8IVW6.

Family and domain databases

Gene3D1.10.150.60. 1 hit.
InterProIPR001606. ARID/BRIGHT_DNA-bd.
IPR023334. REKLES_domain.
[Graphical view]
PfamPF01388. ARID. 1 hit.
[Graphical view]
SMARTSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMSSF46774. SSF46774. 1 hit.
PROSITEPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiARID3B.
GenomeRNAi10620.
NextBio40352.
PROQ8IVW6.
SOURCESearch...

Entry information

Entry nameARI3B_HUMAN
AccessionPrimary (citable) accession number: Q8IVW6
Secondary accession number(s): O95443, Q59HC9, Q6P9C9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: July 9, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM