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Protein

Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

Gene

MCAT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a malonyl moiety from malonyl-CoA to the free thiol group of the phosphopantetheine arm of the mitochondrial ACP protein (NDUFAB1). This suggests the existence of the biosynthesis of fatty acids in mitochondrias.1 Publication

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153By similarity1
Active sitei270By similarity1

GO - Molecular functioni

  • [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • transferase activity Source: UniProtKB

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB
  • metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS02028-MONOMER.
ZFISH:HS02028-MONOMER.
ReactomeiR-HSA-77289. Mitochondrial Fatty Acid Beta-Oxidation.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Malonyl-CoA-acyl carrier protein transacylase, mitochondrial (EC:2.3.1.39)
Short name:
MCT
Alternative name(s):
Mitochondrial malonyl CoA:ACP acyltransferase
Mitochondrial malonyltransferase
[Acyl-carrier-protein] malonyltransferase
Gene namesi
Name:MCAT
Synonyms:MT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:29622. MCAT.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi27349.
OpenTargetsiENSG00000100294.
PharmGKBiPA162395058.

Polymorphism and mutation databases

BioMutaiMCAT.
DMDMi48428076.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
ChainiPRO_000000058922 – 390Malonyl-CoA-acyl carrier protein transacylase, mitochondrialAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei314N6-succinyllysineBy similarity1

Proteomic databases

EPDiQ8IVS2.
MaxQBiQ8IVS2.
PaxDbiQ8IVS2.
PeptideAtlasiQ8IVS2.
PRIDEiQ8IVS2.

PTM databases

iPTMnetiQ8IVS2.
PhosphoSitePlusiQ8IVS2.

Expressioni

Gene expression databases

BgeeiENSG00000100294.
CleanExiHS_MCAT.
ExpressionAtlasiQ8IVS2. baseline and differential.
GenevisibleiQ8IVS2. HS.

Organism-specific databases

HPAiHPA035471.

Interactioni

Protein-protein interaction databases

BioGridi118161. 43 interactors.
IntActiQ8IVS2. 4 interactors.
MINTiMINT-4828919.
STRINGi9606.ENSP00000290429.

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi63 – 67Combined sources5
Turni75 – 83Combined sources9
Helixi87 – 98Combined sources12
Helixi102 – 108Combined sources7
Helixi111 – 115Combined sources5
Helixi117 – 138Combined sources22
Helixi140 – 144Combined sources5
Beta strandi146 – 151Combined sources6
Helixi155 – 162Combined sources8
Helixi168 – 187Combined sources20
Beta strandi191 – 197Combined sources7
Helixi204 – 217Combined sources14
Beta strandi225 – 232Combined sources8
Beta strandi235 – 241Combined sources7
Helixi242 – 250Combined sources9
Helixi252 – 255Combined sources4
Beta strandi259 – 262Combined sources4
Helixi272 – 274Combined sources3
Helixi275 – 286Combined sources12
Beta strandi294 – 298Combined sources5
Turni300 – 302Combined sources3
Beta strandi303 – 305Combined sources3
Helixi309 – 311Combined sources3
Helixi312 – 319Combined sources8
Helixi326 – 333Combined sources8
Beta strandi344 – 352Combined sources9
Helixi353 – 361Combined sources9
Helixi363 – 366Combined sources4
Beta strandi369 – 372Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C2NX-ray1.55A/B60-375[»]
ProteinModelPortaliQ8IVS2.
SMRiQ8IVS2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IVS2.

Family & Domainsi

Sequence similaritiesi

Belongs to the type II malonyltransferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2926. Eukaryota.
COG0331. LUCA.
GeneTreeiENSGT00390000013715.
HOGENOMiHOG000038000.
HOVERGENiHBG051540.
InParanoidiQ8IVS2.
KOiK00645.
OMAiNEAYHNK.
OrthoDBiEOG091G09QO.
PhylomeDBiQ8IVS2.
TreeFamiTF313401.

Family and domain databases

Gene3Di3.40.366.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IVS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVRVARVAW VRGLGASYRR GASSFPVPPP GAQGVAELLR DATGAEEEAP
60 70 80 90 100
WAATERRMPG QCSVLLFPGQ GSQVVGMGRG LLNYPRVREL YAAARRVLGY
110 120 130 140 150
DLLELSLHGP QETLDRTVHC QPAIFVASLA AVEKLHHLQP SVIENCVAAA
160 170 180 190 200
GFSVGEFAAL VFAGAMEFAE GLYAVKIRAE AMQEASEAVP SGMLSVLGQP
210 220 230 240 250
QSKFNFACLE AREHCKSLGI ENPVCEVSNY LFPDCRVISG HQEALRFLQK
260 270 280 290 300
NSSKFHFRRT RMLPVSGAFH TRLMEPAVEP LTQALKAVDI KKPLVSVYSN
310 320 330 340 350
VHAHRYRHPG HIHKLLAQQL VSPVKWEQTM HAIYERKKGR GFPQTFEVGP
360 370 380 390
GRQLGAILKS CNMQAWKSYS AVDVLQTLEH VDLDPQEPPR
Length:390
Mass (Da):42,962
Last modified:June 7, 2004 - v2
Checksum:i5CAF7AE9DFF36A21
GO
Isoform 2 (identifier: Q8IVS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     172-180: LYAVKIRAE → STVSPEEFL
     181-390: Missing.

Show »
Length:180
Mass (Da):19,176
Checksum:i8DA3FDA905D219C7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048183303A → G.1 PublicationCorresponds to variant rs13815dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010517172 – 180LYAVKIRAE → STVSPEEFL in isoform 2. 3 Publications9
Alternative sequenceiVSP_010518181 – 390Missing in isoform 2. 3 PublicationsAdd BLAST210

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL359401 mRNA. Translation: CAB94789.1.
AL359403 mRNA. Translation: CAB94790.1.
AK314059 mRNA. Translation: BAG36765.1.
AL022237 Genomic DNA. Translation: CAQ07851.1.
AL022237 Genomic DNA. Translation: CAA18261.1.
CH471138 Genomic DNA. Translation: EAW73286.1.
BC030985 mRNA. Translation: AAH30985.1.
BC042195 mRNA. Translation: AAH42195.2.
CCDSiCCDS14045.1. [Q8IVS2-2]
CCDS33660.1. [Q8IVS2-1]
RefSeqiNP_055322.1. NM_014507.3. [Q8IVS2-2]
NP_775738.3. NM_173467.4. [Q8IVS2-1]
UniGeneiHs.349111.

Genome annotation databases

EnsembliENST00000290429; ENSP00000290429; ENSG00000100294. [Q8IVS2-1]
ENST00000327555; ENSP00000331306; ENSG00000100294. [Q8IVS2-2]
GeneIDi27349.
KEGGihsa:27349.
UCSCiuc003bdl.2. human. [Q8IVS2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL359401 mRNA. Translation: CAB94789.1.
AL359403 mRNA. Translation: CAB94790.1.
AK314059 mRNA. Translation: BAG36765.1.
AL022237 Genomic DNA. Translation: CAQ07851.1.
AL022237 Genomic DNA. Translation: CAA18261.1.
CH471138 Genomic DNA. Translation: EAW73286.1.
BC030985 mRNA. Translation: AAH30985.1.
BC042195 mRNA. Translation: AAH42195.2.
CCDSiCCDS14045.1. [Q8IVS2-2]
CCDS33660.1. [Q8IVS2-1]
RefSeqiNP_055322.1. NM_014507.3. [Q8IVS2-2]
NP_775738.3. NM_173467.4. [Q8IVS2-1]
UniGeneiHs.349111.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C2NX-ray1.55A/B60-375[»]
ProteinModelPortaliQ8IVS2.
SMRiQ8IVS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118161. 43 interactors.
IntActiQ8IVS2. 4 interactors.
MINTiMINT-4828919.
STRINGi9606.ENSP00000290429.

PTM databases

iPTMnetiQ8IVS2.
PhosphoSitePlusiQ8IVS2.

Polymorphism and mutation databases

BioMutaiMCAT.
DMDMi48428076.

Proteomic databases

EPDiQ8IVS2.
MaxQBiQ8IVS2.
PaxDbiQ8IVS2.
PeptideAtlasiQ8IVS2.
PRIDEiQ8IVS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290429; ENSP00000290429; ENSG00000100294. [Q8IVS2-1]
ENST00000327555; ENSP00000331306; ENSG00000100294. [Q8IVS2-2]
GeneIDi27349.
KEGGihsa:27349.
UCSCiuc003bdl.2. human. [Q8IVS2-1]

Organism-specific databases

CTDi27349.
DisGeNETi27349.
GeneCardsiMCAT.
HGNCiHGNC:29622. MCAT.
HPAiHPA035471.
MIMi614479. gene.
neXtProtiNX_Q8IVS2.
OpenTargetsiENSG00000100294.
PharmGKBiPA162395058.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2926. Eukaryota.
COG0331. LUCA.
GeneTreeiENSGT00390000013715.
HOGENOMiHOG000038000.
HOVERGENiHBG051540.
InParanoidiQ8IVS2.
KOiK00645.
OMAiNEAYHNK.
OrthoDBiEOG091G09QO.
PhylomeDBiQ8IVS2.
TreeFamiTF313401.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciMetaCyc:HS02028-MONOMER.
ZFISH:HS02028-MONOMER.
ReactomeiR-HSA-77289. Mitochondrial Fatty Acid Beta-Oxidation.

Miscellaneous databases

ChiTaRSiMCAT. human.
EvolutionaryTraceiQ8IVS2.
GeneWikiiMCAT_(gene).
GenomeRNAii27349.
PROiQ8IVS2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100294.
CleanExiHS_MCAT.
ExpressionAtlasiQ8IVS2. baseline and differential.
GenevisibleiQ8IVS2. HS.

Family and domain databases

Gene3Di3.40.366.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABD_HUMAN
AccessioniPrimary (citable) accession number: Q8IVS2
Secondary accession number(s): B0QY72, O95510, O95511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.