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Protein

Carboxypeptidase O

Gene

CPO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.1 Publication

Cofactori

Zn2+By similarity

Enzyme regulationi

Strongly inhibited by potato carboxypeptidase inhibitor, and the chelating agents EDTA and 1,10-phenanthroline. Also inhibited by compounds with multiple carboxylic acid groups such as citrate and succinate, and to a lesser exent the amino acids aspartate and glutamate. Not significantly inhibited by benzylsuccinic acid.1 Publication

Kineticsi

  1. KM=325 µM for 3-(2-furyl)acryloyl-Glu-Glu (at pH 7.5)1 Publication
  2. KM=284 µM for 3-(2-furyl)acryloyl-Phe-Phe (at pH 7.5)1 Publication
  3. KM=549 µM for 3-(2-furyl)acryloyl-Phe-Ala (at pH 7.5)1 Publication
  4. KM=614 µM for 3-(2-furyl)acryloyl-Lys-Ala (at pH 7.5)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi108Zinc; catalyticBy similarity1
    Metal bindingi111Zinc; catalyticBy similarity1
    Metal bindingi236Zinc; catalyticBy similarity1
    Active sitei310Proton donor/acceptorBy similarity1

    GO - Molecular functioni

    • metallocarboxypeptidase activity Source: UniProtKB
    • zinc ion binding Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000144410-MONOMER.

    Protein family/group databases

    MEROPSiM14.021.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxypeptidase OImported (EC:3.4.17.-1 Publication)
    Short name:
    CPOImported
    Gene namesi
    Name:CPOImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:21011. CPO.

    Subcellular locationi

    • Apical cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

    GO - Cellular componenti

    • anchored component of plasma membrane Source: UniProtKB
    • apical plasma membrane Source: UniProtKB-SubCell
    • extracellular space Source: GO_Central
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi130749.
    OpenTargetsiENSG00000144410.
    PharmGKBiPA164741367.

    Polymorphism and mutation databases

    BioMutaiCPO.
    DMDMi74723635.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 201 PublicationAdd BLAST20
    ChainiPRO_000025240121 – 352Carboxypeptidase OCuratedAdd BLAST332
    PropeptideiPRO_0000437881353 – 374Removed in mature formCuratedAdd BLAST22

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi132N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi174N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
    Lipidationi352GPI-anchor amidated aspartateSequence analysis1

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ8IVL8.
    PeptideAtlasiQ8IVL8.
    PRIDEiQ8IVL8.

    PTM databases

    iPTMnetiQ8IVL8.
    PhosphoSitePlusiQ8IVL8.

    Expressioni

    Tissue specificityi

    Detected in enterocytes of the ileum.1 Publication

    Gene expression databases

    BgeeiENSG00000144410.
    CleanExiHS_CPO.
    GenevisibleiQ8IVL8. HS.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000272852.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8IVL8.
    SMRiQ8IVL8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M14 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG2650. Eukaryota.
    COG2866. LUCA.
    GeneTreeiENSGT00760000119103.
    HOGENOMiHOG000252967.
    HOVERGENiHBG050815.
    InParanoidiQ8IVL8.
    OMAiTKKIIWM.
    OrthoDBiEOG091G0HUI.
    PhylomeDBiQ8IVL8.
    TreeFamiTF317197.

    Family and domain databases

    CDDicd06247. M14_CPO. 1 hit.
    InterProiIPR033850. Carboxypeptidase_O.
    IPR000834. Peptidase_M14.
    [Graphical view]
    PfamiPF00246. Peptidase_M14. 1 hit.
    [Graphical view]
    PRINTSiPR00765. CRBOXYPTASEA.
    SMARTiSM00631. Zn_pept. 1 hit.
    [Graphical view]
    PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8IVL8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKPLLETLYL LGMLVPGGLG YDRSLAQHRQ EIVDKSVSPW SLETYSYNIY
    60 70 80 90 100
    HPMGEIYEWM REISEKYKEV VTQHFLGVTY ETHPMYYLKI SQPSGNPKKI
    110 120 130 140 150
    IWMDCGIHAR EWIAPAFCQW FVKEILQNHK DNSSIRKLLR NLDFYVLPVL
    160 170 180 190 200
    NIDGYIYTWT TDRLWRKSRS PHNNGTCFGT DLNRNFNASW CSIGASRNCQ
    210 220 230 240 250
    DQTFCGTGPV SEPETKAVAS FIESKKDDIL CFLTMHSYGQ LILTPYGYTK
    260 270 280 290 300
    NKSSNHPEMI QVGQKAANAL KAKYGTNYRV GSSADILYAS SGSSRDWARD
    310 320 330 340 350
    IGIPFSYTFE LRDSGTYGFV LPEAQIQPTC EETMEAVLSV LDDVYAKHWH
    360 370
    SDSAGRVTSA TMLLGLLVSC MSLL
    Length:374
    Mass (Da):42,529
    Last modified:March 1, 2003 - v1
    Checksum:i404C373BB841AAD2
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_02785085M → I.1 PublicationCorresponds to variant rs13420911dbSNPEnsembl.1
    Natural variantiVAR_027851134S → R.1 PublicationCorresponds to variant rs11903403dbSNPEnsembl.1
    Natural variantiVAR_036012273K → N in a colorectal cancer sample; somatic mutation. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ422118 mRNA. Translation: CAD19478.1.
    AC019052 Genomic DNA. Translation: AAX93277.1.
    BC112076 mRNA. Translation: AAI12077.1.
    BC112078 mRNA. Translation: AAI12079.1.
    BK000189 mRNA. Translation: DAA00036.1.
    CCDSiCCDS2372.1.
    RefSeqiNP_775100.1. NM_173077.2.
    XP_016858861.1. XM_017003372.1.
    UniGeneiHs.684103.

    Genome annotation databases

    EnsembliENST00000272852; ENSP00000272852; ENSG00000144410.
    GeneIDi130749.
    KEGGihsa:130749.
    UCSCiuc002vby.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ422118 mRNA. Translation: CAD19478.1.
    AC019052 Genomic DNA. Translation: AAX93277.1.
    BC112076 mRNA. Translation: AAI12077.1.
    BC112078 mRNA. Translation: AAI12079.1.
    BK000189 mRNA. Translation: DAA00036.1.
    CCDSiCCDS2372.1.
    RefSeqiNP_775100.1. NM_173077.2.
    XP_016858861.1. XM_017003372.1.
    UniGeneiHs.684103.

    3D structure databases

    ProteinModelPortaliQ8IVL8.
    SMRiQ8IVL8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi9606.ENSP00000272852.

    Protein family/group databases

    MEROPSiM14.021.

    PTM databases

    iPTMnetiQ8IVL8.
    PhosphoSitePlusiQ8IVL8.

    Polymorphism and mutation databases

    BioMutaiCPO.
    DMDMi74723635.

    Proteomic databases

    PaxDbiQ8IVL8.
    PeptideAtlasiQ8IVL8.
    PRIDEiQ8IVL8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000272852; ENSP00000272852; ENSG00000144410.
    GeneIDi130749.
    KEGGihsa:130749.
    UCSCiuc002vby.2. human.

    Organism-specific databases

    CTDi130749.
    DisGeNETi130749.
    GeneCardsiCPO.
    HGNCiHGNC:21011. CPO.
    MIMi609563. gene.
    neXtProtiNX_Q8IVL8.
    OpenTargetsiENSG00000144410.
    PharmGKBiPA164741367.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2650. Eukaryota.
    COG2866. LUCA.
    GeneTreeiENSGT00760000119103.
    HOGENOMiHOG000252967.
    HOVERGENiHBG050815.
    InParanoidiQ8IVL8.
    OMAiTKKIIWM.
    OrthoDBiEOG091G0HUI.
    PhylomeDBiQ8IVL8.
    TreeFamiTF317197.

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000144410-MONOMER.

    Miscellaneous databases

    GenomeRNAii130749.
    PROiQ8IVL8.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000144410.
    CleanExiHS_CPO.
    GenevisibleiQ8IVL8. HS.

    Family and domain databases

    CDDicd06247. M14_CPO. 1 hit.
    InterProiIPR033850. Carboxypeptidase_O.
    IPR000834. Peptidase_M14.
    [Graphical view]
    PfamiPF00246. Peptidase_M14. 1 hit.
    [Graphical view]
    PRINTSiPR00765. CRBOXYPTASEA.
    SMARTiSM00631. Zn_pept. 1 hit.
    [Graphical view]
    PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCBPO_HUMAN
    AccessioniPrimary (citable) accession number: Q8IVL8
    Secondary accession number(s): Q2M277, Q7RTW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: March 1, 2003
    Last modified: November 30, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.