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Protein

Carboxypeptidase O

Gene

CPO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.1 Publication

Cofactori

Zn2+By similarity

Enzyme regulationi

Strongly inhibited by potato carboxypeptidase inhibitor, and the chelating agents EDTA and 1,10-phenanthroline. Also inhibited by compounds with multiple carboxylic acid groups such as citrate and succinate, and to a lesser exent the amino acids aspartate and glutamate. Not significantly inhibited by benzylsuccinic acid.1 Publication

Kineticsi

  1. KM=325 µM for 3-(2-furyl)acryloyl-Glu-Glu (at pH 7.5)1 Publication
  2. KM=284 µM for 3-(2-furyl)acryloyl-Phe-Phe (at pH 7.5)1 Publication
  3. KM=549 µM for 3-(2-furyl)acryloyl-Phe-Ala (at pH 7.5)1 Publication
  4. KM=614 µM for 3-(2-furyl)acryloyl-Lys-Ala (at pH 7.5)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi108Zinc; catalyticBy similarity1
    Metal bindingi111Zinc; catalyticBy similarity1
    Metal bindingi236Zinc; catalyticBy similarity1
    Active sitei310Proton donor/acceptorBy similarity1

    GO - Molecular functioni

    • metallocarboxypeptidase activity Source: UniProtKB
    • zinc ion binding Source: InterPro

    Keywordsi

    Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
    LigandMetal-binding, Zinc

    Protein family/group databases

    MEROPSiM14.021

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxypeptidase OImported (EC:3.4.17.-1 Publication)
    Short name:
    CPOImported
    Gene namesi
    Name:CPOImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000144410.4
    HGNCiHGNC:21011 CPO
    MIMi609563 gene
    neXtProtiNX_Q8IVL8

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi130749
    OpenTargetsiENSG00000144410
    PharmGKBiPA164741367

    Polymorphism and mutation databases

    BioMutaiCPO
    DMDMi74723635

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 201 PublicationAdd BLAST20
    ChainiPRO_000025240121 – 352Carboxypeptidase OCuratedAdd BLAST332
    PropeptideiPRO_0000437881353 – 374Removed in mature formCuratedAdd BLAST22

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi132N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi187N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
    Lipidationi352GPI-anchor amidated aspartateSequence analysis1

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ8IVL8
    PeptideAtlasiQ8IVL8
    PRIDEiQ8IVL8

    PTM databases

    iPTMnetiQ8IVL8
    PhosphoSitePlusiQ8IVL8

    Expressioni

    Tissue specificityi

    Detected in enterocytes of the ileum.1 Publication

    Gene expression databases

    BgeeiENSG00000144410
    CleanExiHS_CPO
    GenevisibleiQ8IVL8 HS

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000272852

    Structurei

    Secondary structure

    1374
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi53 – 66Combined sources14
    Turni67 – 70Combined sources4
    Beta strandi71 – 78Combined sources8
    Beta strandi84 – 90Combined sources7
    Beta strandi99 – 105Combined sources7
    Helixi113 – 128Combined sources16
    Turni129 – 131Combined sources3
    Helixi133 – 141Combined sources9
    Beta strandi142 – 148Combined sources7
    Helixi152 – 160Combined sources9
    Turni173 – 176Combined sources4
    Helixi182 – 184Combined sources3
    Beta strandi186 – 189Combined sources4
    Beta strandi192 – 194Combined sources3
    Helixi213 – 225Combined sources13
    Helixi226 – 228Combined sources3
    Beta strandi229 – 236Combined sources8
    Beta strandi241 – 245Combined sources5
    Helixi256 – 274Combined sources19
    Beta strandi279 – 282Combined sources4
    Helixi283 – 286Combined sources4
    Helixi294 – 300Combined sources7
    Beta strandi304 – 310Combined sources7
    Beta strandi314 – 317Combined sources4
    Helixi323 – 325Combined sources3
    Helixi326 – 346Combined sources21

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5MRVX-ray1.85A/B21-349[»]
    ProteinModelPortaliQ8IVL8
    SMRiQ8IVL8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M14 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG2650 Eukaryota
    COG2866 LUCA
    GeneTreeiENSGT00760000119103
    HOGENOMiHOG000252967
    HOVERGENiHBG050815
    InParanoidiQ8IVL8
    OMAiFCGTGPV
    OrthoDBiEOG091G0HUI
    PhylomeDBiQ8IVL8
    TreeFamiTF317197

    Family and domain databases

    CDDicd06247 M14_CPO, 1 hit
    InterProiView protein in InterPro
    IPR033850 Carboxypeptidase_O
    IPR000834 Peptidase_M14
    PfamiView protein in Pfam
    PF00246 Peptidase_M14, 1 hit
    PRINTSiPR00765 CRBOXYPTASEA
    SMARTiView protein in SMART
    SM00631 Zn_pept, 1 hit
    PROSITEiView protein in PROSITE
    PS00132 CARBOXYPEPT_ZN_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8IVL8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKPLLETLYL LGMLVPGGLG YDRSLAQHRQ EIVDKSVSPW SLETYSYNIY
    60 70 80 90 100
    HPMGEIYEWM REISEKYKEV VTQHFLGVTY ETHPMYYLKI SQPSGNPKKI
    110 120 130 140 150
    IWMDCGIHAR EWIAPAFCQW FVKEILQNHK DNSSIRKLLR NLDFYVLPVL
    160 170 180 190 200
    NIDGYIYTWT TDRLWRKSRS PHNNGTCFGT DLNRNFNASW CSIGASRNCQ
    210 220 230 240 250
    DQTFCGTGPV SEPETKAVAS FIESKKDDIL CFLTMHSYGQ LILTPYGYTK
    260 270 280 290 300
    NKSSNHPEMI QVGQKAANAL KAKYGTNYRV GSSADILYAS SGSSRDWARD
    310 320 330 340 350
    IGIPFSYTFE LRDSGTYGFV LPEAQIQPTC EETMEAVLSV LDDVYAKHWH
    360 370
    SDSAGRVTSA TMLLGLLVSC MSLL
    Length:374
    Mass (Da):42,529
    Last modified:March 1, 2003 - v1
    Checksum:i404C373BB841AAD2
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_02785085M → I1 PublicationCorresponds to variant dbSNP:rs13420911Ensembl.1
    Natural variantiVAR_027851134S → R1 PublicationCorresponds to variant dbSNP:rs11903403Ensembl.1
    Natural variantiVAR_036012273K → N in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs896000260Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ422118 mRNA Translation: CAD19478.1
    AC019052 Genomic DNA Translation: AAX93277.1
    BC112076 mRNA Translation: AAI12077.1
    BC112078 mRNA Translation: AAI12079.1
    BK000189 mRNA Translation: DAA00036.1
    CCDSiCCDS2372.1
    RefSeqiNP_775100.1, NM_173077.2
    XP_016858861.1, XM_017003372.1
    UniGeneiHs.684103

    Genome annotation databases

    EnsembliENST00000272852; ENSP00000272852; ENSG00000144410
    GeneIDi130749
    KEGGihsa:130749
    UCSCiuc002vby.2 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiCBPO_HUMAN
    AccessioniPrimary (citable) accession number: Q8IVL8
    Secondary accession number(s): Q2M277, Q7RTW7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: March 1, 2003
    Last modified: March 28, 2018
    This is version 116 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health