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Protein

Formin-like protein 3

Gene

FMNL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. Required for developmental angiogenesis (By similarity). In this process, required for microtubule reorganization and for efficient endothelial cell elongation. In quiescent endothelial cells, triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: InterPro
  • angiogenesis Source: UniProtKB-KW
  • cell migration Source: UniProtKB
  • cytoskeleton organization Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Angiogenesis

Enzyme and pathway databases

ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Formin-like protein 3
Alternative name(s):
Formin homology 2 domain-containing protein 3
WW domain-binding protein 3
Short name:
WBP-3
Gene namesi
Name:FMNL3
Synonyms:FHOD3, FRL2, KIAA2014, WBP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:23698. FMNL3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi91010.
OpenTargetsiENSG00000161791.
PharmGKBiPA134992328.

Polymorphism and mutation databases

BioMutaiFMNL3.
DMDMi148886617.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002890952 – 1028Formin-like protein 3Add BLAST1027

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei95PhosphothreonineBy similarity1
Modified residuei174PhosphoserineCombined sources1
Modified residuei1014PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ8IVF7.
MaxQBiQ8IVF7.
PaxDbiQ8IVF7.
PeptideAtlasiQ8IVF7.
PRIDEiQ8IVF7.

PTM databases

iPTMnetiQ8IVF7.
PhosphoSitePlusiQ8IVF7.

Expressioni

Tissue specificityi

Expressed in endothelial cells.1 Publication

Gene expression databases

BgeeiENSG00000161791.
CleanExiHS_FHOD3.
HS_FMNL3.
ExpressionAtlasiQ8IVF7. baseline and differential.
GenevisibleiQ8IVF7. HS.

Organism-specific databases

HPAiHPA002552.
HPA023201.

Interactioni

Subunit structurei

Interacts with SRGAP2 (via SH3 domain).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124787. 8 interactors.
IntActiQ8IVF7. 3 interactors.
MINTiMINT-1182764.
STRINGi9606.ENSP00000335655.

Structurei

3D structure databases

ProteinModelPortaliQ8IVF7.
SMRiQ8IVF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 472GBD/FH3PROSITE-ProRule annotationAdd BLAST447
Domaini561 – 951FH2PROSITE-ProRule annotationAdd BLAST391
Domaini986 – 1018DADAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi499 – 548Pro-richAdd BLAST50

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1923. Eukaryota.
ENOG410Y3DT. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000231209.
HOVERGENiHBG053118.
InParanoidiQ8IVF7.
OMAiRCHLEPN.
PhylomeDBiQ8IVF7.
TreeFamiTF325155.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 2 hits.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IVF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNLESAEGV PGEPPSVPLL LPPGKMPMPE PCELEERFAL VLSSMNLPPD
60 70 80 90 100
KARLLRQYDN EKKWDLICDQ ERFQVKNPPH TYIQKLQSFL DPSVTRKKFR
110 120 130 140 150
RRVQESTKVL RELEISLRTN HIGWVREFLN DENKGLDVLV DYLSFAQCSV
160 170 180 190 200
MFDFEGLESG DDGAFDKLRS WSRSIEDLQP PSALSAPFTN SLARSARQSV
210 220 230 240 250
LRYSTLPGRR ALKNSRLVSQ KDDVHVCILC LRAIMNYQYG FNLVMSHPHA
260 270 280 290 300
VNEIALSLNN KNPRTKALVL ELLAAVCLVR GGHEIILAAF DNFKEVCKEL
310 320 330 340 350
HRFEKLMEYF RNEDSNIDFM VACMQFINIV VHSVEDMNFR VHLQYEFTKL
360 370 380 390 400
GLEEFLQKSR HTESEKLQVQ IQAYLDNVFD VGGLLEDAET KNVALEKVEE
410 420 430 440 450
LEEHVSHLTE KLLDLENENM MRVAELEKQL LQREKELESI KETYENTSHQ
460 470 480 490 500
VHTLRRLIKE KEEAFQRRCH LEPNVRGLES VDSEALARVG PAELSEGMPP
510 520 530 540 550
SDLDLLAPAP PPEEVLPLPP PPAPPLPPPP PPLPDKCPPA PPLPGAAPSV
560 570 580 590 600
VLTVGLSAIR IKKPIKTKFR LPVFNWTALK PNQISGTVFS ELDDEKILED
610 620 630 640 650
LDLDKFEELF KTKAQGPALD LICSKNKTAQ KAASKVTLLE ANRAKNLAIT
660 670 680 690 700
LRKAGRSAEE ICRAIHTFDL QTLPVDFVEC LMRFLPTEAE VKLLRQYERE
710 720 730 740 750
RQPLEELAAE DRFMLLFSKV ERLTQRMAGM AFLGNFQDNL QMLTPQLNAI
760 770 780 790 800
IAASASVKSS QKLKQMLEII LALGNYMNSS KRGAVYGFKL QSLDLLLDTK
810 820 830 840 850
STDRKMTLLH FIALTVKEKY PDLANFWHEL HFVEKAAAVS LENVLLDVKE
860 870 880 890 900
LGRGMELIRR ECSIHDNSVL RNFLSTNEGK LDKLQRDAKT AEEAYNAVVR
910 920 930 940 950
YFGESPKTTP PSVFFPVFVR FIRSYKEAEQ ENEARKKQEE VMREKQLAQE
960 970 980 990 1000
AKKLDAKTPS QRNKWQQQEL IAELRRRQAK EHRPVYEGKD GTIEDIITVL
1010 1020
KSVPFTARTA KRGSRFFCDA AHHDESNC
Length:1,028
Mass (Da):117,213
Last modified:May 29, 2007 - v3
Checksum:i79A574506A0FD6E1
GO
Isoform 2 (identifier: Q8IVF7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-202: Missing.
     999-1028: VLKSVPFTARTAKRGSRFFCDAAHHDESNC → GLHCQPMVVRHQARSAAPPSGPPRAPGPH

Show »
Length:976
Mass (Da):111,259
Checksum:i7A242E36225DC7C1
GO
Isoform 3 (identifier: Q8IVF7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     999-1028: VLKSVPFTARTAKRGSRFFCDAAHHDESNC → GLHCQPMVVRHQARSAAPPSGPPRAPGPH

Show »
Length:1,027
Mass (Da):116,871
Checksum:iA71C76EB61DF3BA1
GO

Sequence cautioni

The sequence BAC23110 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025892152 – 202Missing in isoform 2. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_025893999 – 1028VLKSV…DESNC → GLHCQPMVVRHQARSAAPPS GPPRAPGPH in isoform 3 and isoform 2. 3 PublicationsAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095934 mRNA. Translation: BAC23110.2. Different initiation.
AK128195 mRNA. Translation: BAC87319.1.
AC020612 Genomic DNA. No translation available.
BC159100 mRNA. Translation: AAI59101.1.
CCDSiCCDS41780.1. [Q8IVF7-2]
CCDS44874.1. [Q8IVF7-3]
RefSeqiNP_783863.4. NM_175736.4. [Q8IVF7-3]
NP_944489.2. NM_198900.2. [Q8IVF7-2]
XP_005269275.1. XM_005269218.2. [Q8IVF7-1]
UniGeneiHs.179838.

Genome annotation databases

EnsembliENST00000335154; ENSP00000335655; ENSG00000161791. [Q8IVF7-3]
ENST00000352151; ENSP00000344311; ENSG00000161791. [Q8IVF7-2]
GeneIDi91010.
KEGGihsa:91010.
UCSCiuc001ruv.2. human. [Q8IVF7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095934 mRNA. Translation: BAC23110.2. Different initiation.
AK128195 mRNA. Translation: BAC87319.1.
AC020612 Genomic DNA. No translation available.
BC159100 mRNA. Translation: AAI59101.1.
CCDSiCCDS41780.1. [Q8IVF7-2]
CCDS44874.1. [Q8IVF7-3]
RefSeqiNP_783863.4. NM_175736.4. [Q8IVF7-3]
NP_944489.2. NM_198900.2. [Q8IVF7-2]
XP_005269275.1. XM_005269218.2. [Q8IVF7-1]
UniGeneiHs.179838.

3D structure databases

ProteinModelPortaliQ8IVF7.
SMRiQ8IVF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124787. 8 interactors.
IntActiQ8IVF7. 3 interactors.
MINTiMINT-1182764.
STRINGi9606.ENSP00000335655.

PTM databases

iPTMnetiQ8IVF7.
PhosphoSitePlusiQ8IVF7.

Polymorphism and mutation databases

BioMutaiFMNL3.
DMDMi148886617.

Proteomic databases

EPDiQ8IVF7.
MaxQBiQ8IVF7.
PaxDbiQ8IVF7.
PeptideAtlasiQ8IVF7.
PRIDEiQ8IVF7.

Protocols and materials databases

DNASUi91010.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335154; ENSP00000335655; ENSG00000161791. [Q8IVF7-3]
ENST00000352151; ENSP00000344311; ENSG00000161791. [Q8IVF7-2]
GeneIDi91010.
KEGGihsa:91010.
UCSCiuc001ruv.2. human. [Q8IVF7-1]

Organism-specific databases

CTDi91010.
DisGeNETi91010.
GeneCardsiFMNL3.
HGNCiHGNC:23698. FMNL3.
HPAiHPA002552.
HPA023201.
MIMi616288. gene.
neXtProtiNX_Q8IVF7.
OpenTargetsiENSG00000161791.
PharmGKBiPA134992328.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1923. Eukaryota.
ENOG410Y3DT. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000231209.
HOVERGENiHBG053118.
InParanoidiQ8IVF7.
OMAiRCHLEPN.
PhylomeDBiQ8IVF7.
TreeFamiTF325155.

Enzyme and pathway databases

ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

ChiTaRSiFMNL3. human.
GeneWikiiFMNL3.
GenomeRNAii91010.
PROiQ8IVF7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161791.
CleanExiHS_FHOD3.
HS_FMNL3.
ExpressionAtlasiQ8IVF7. baseline and differential.
GenevisibleiQ8IVF7. HS.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 2 hits.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFMNL3_HUMAN
AccessioniPrimary (citable) accession number: Q8IVF7
Secondary accession number(s): B0JZA7, Q6ZRJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 30, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.