Q8IV61 (GRP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras guanyl-releasing protein 3

Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor III
Guanine nucleotide exchange factor for Rap1

Gene names

Name:	RASGRP3
Synonyms:	GRP3, KIAA0846

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) for Ras and Rap1. Ref.6
Sequence similarities	Belongs to the RASGRP family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain.
Sequence caution	The sequence BAA74869.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Calcium Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Non-traceable author statement Ref.6. Source: UniProtKB Ras protein signal transduction Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Traceable author statement Ref.6. Source: UniProtKB
Cellular_component	guanyl-nucleotide exchange factor complex Inferred from electronic annotation. Source: Compara integral to plasma membrane Non-traceable author statement Ref.6. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	Rap GTPase activator activity Inferred from direct assay Ref.6. Source: UniProtKB Ras guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: Compara calcium ion binding Non-traceable author statement Ref.6. Source: UniProtKB diacylglycerol binding Non-traceable author statement Ref.6. Source: UniProtKB guanyl-nucleotide exchange factor activity Traceable author statement. Source: Reactome signal transducer activity Traceable author statement Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Ras guanyl-releasing protein 3		PRO_0000068883
Regions
	Domain	3 – 125	123	N-terminal Ras-GEF
	Domain	152 – 383	232	Ras-GEF
	Domain	420 – 455	36	EF-hand 1
	Domain	449 – 484	36	EF-hand 2
	Calcium binding	433 – 444	12	1 Potential
	Calcium binding	462 – 473	12	2 Potential
	Zinc finger	494 – 544	51	Phorbol-ester/DAG-type
Natural variations
	Natural variant	393	1	T → A. Corresponds to variant rs13388394 [ dbSNP | Ensembl ].		VAR_051901
Experimental info
	Sequence conflict	388	1	Missing in BAA74869. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IV61 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 747BBAA2CCAC2D12	FASTA	690	78,332
	10 20 30 40 50 60 MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR 70 80 90 100 110 120 NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI 130 140 150 160 170 180 SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV 190 200 210 220 230 240 IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT 250 260 270 280 290 300 LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP 310 320 330 340 350 360 ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL 370 380 390 400 410 420 TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH 430 440 450 460 470 480 IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR 490 500 510 520 530 540 AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL 550 560 570 580 590 600 VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK 610 620 630 640 650 660 ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV 670 680 690 HAGVDVVDRG TEFELDQDEG EETRQDGEDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Endometrium.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction." Rebhun J.F., Castro A.F., Quilliam L.A. J. Biol. Chem. 275:34901-34908(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020653 mRNA. Translation: BAA74869.2. Different initiation. BX647990 mRNA. No translation available. AC020594 Genomic DNA. Translation: AAY15037.1. CH471053 Genomic DNA. Translation: EAX00433.1. BC027849 mRNA. Translation: AAH27849.1.
IPI	IPI00171874.
RefSeq	NP_001132960.1. NM_001139488.1. NP_056191.1. NM_015376.2. NP_733772.1. NM_170672.2.
UniGene	Hs.143674.
3D structure databases
ProteinModelPortal	Q8IV61.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IV61. 2 interactions.
STRING	9606.ENSP00000384192.
PTM databases
PhosphoSite	Q8IV61.
Polymorphism databases
DMDM	34395670.
Proteomic databases
PaxDb	Q8IV61.
PRIDE	Q8IV61.
Protocols and materials databases
DNASU	25780.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000402538; ENSP00000385886; ENSG00000152689. ENST00000403687; ENSP00000384192; ENSG00000152689.
GeneID	25780.
KEGG	hsa:25780.
UCSC	uc002rox.3. human.
Organism-specific databases
CTD	25780.
GeneCards	GC02P033661.
H-InvDB	HIX0001961.
HGNC	HGNC:14545. RASGRP3.
MIM	609531. gene.
neXtProt	NX_Q8IV61.
PharmGKB	PA134937092.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG256085.
HOGENOM	HOG000293171.
HOVERGEN	HBG007513.
InParanoid	Q8IV61.
KO	K12362.
OMA	IPSYDWM.
OrthoDB	EOG4S1T6P.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q8IV61.
Bgee	Q8IV61.
CleanEx	HS_RASGRP3.
Genevestigator	Q8IV61.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.10.840.10. 1 hit.
InterPro	IPR020454. DAG/PE-bd. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000651. Ras-like_Gua-exchang_fac_N. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF13202. EF_hand_3. 1 hit. PF13405. EF_hand_4. 1 hit. PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. [Graphical view]
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00054. EFh. 2 hits. SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit.
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8IV61.
ChEMBL	CHEMBL3638.
GenomeRNAi	25780.
NextBio	46931.
SOURCE	Search...

Entry information

Entry name

GRP3_HUMAN

Accession

Primary (citable) accession number: Q8IV61
Secondary accession number(s): O94931, Q53SD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.