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Q8IV61

- GRP3_HUMAN

UniProt

Q8IV61 - GRP3_HUMAN

Protein

Ras guanyl-releasing protein 3

Gene

RASGRP3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Guanine nucleotide exchange factor (GEF) for Ras and Rap1.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi433 – 444121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi462 – 473122PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri494 – 54451Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. diacylglycerol binding Source: UniProtKB
    3. guanyl-nucleotide exchange factor activity Source: Reactome
    4. Rap GTPase activator activity Source: UniProtKB
    5. Ras guanyl-nucleotide exchange factor activity Source: Ensembl
    6. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. MAPK cascade Source: UniProtKB
    2. positive regulation of GTPase activity Source: GOC
    3. positive regulation of Rap GTPase activity Source: GOC
    4. Ras protein signal transduction Source: Ensembl
    5. small GTPase mediated signal transduction Source: UniProtKB

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_118778. Activation of RAS in B cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras guanyl-releasing protein 3
    Alternative name(s):
    Calcium and DAG-regulated guanine nucleotide exchange factor III
    Guanine nucleotide exchange factor for Rap1
    Gene namesi
    Name:RASGRP3
    Synonyms:GRP3, KIAA0846
    OrganismiHomo sapiens (Human)Imported
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:14545. RASGRP3.

    Subcellular locationi

    GO - Cellular componenti

    1. guanyl-nucleotide exchange factor complex Source: Ensembl
    2. integral component of plasma membrane Source: UniProtKB
    3. perinuclear region of cytoplasm Source: Ensembl
    4. plasma membrane Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134937092.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 690690Ras guanyl-releasing protein 3PRO_0000068883Add
    BLAST

    Proteomic databases

    PaxDbiQ8IV61.
    PRIDEiQ8IV61.

    PTM databases

    PhosphoSiteiQ8IV61.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8IV61.
    BgeeiQ8IV61.
    CleanExiHS_RASGRP3.
    GenevestigatoriQ8IV61.

    Interactioni

    Protein-protein interaction databases

    BioGridi117315. 10 interactions.
    IntActiQ8IV61. 2 interactions.
    STRINGi9606.ENSP00000384192.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8IV61.
    SMRiQ8IV61. Positions 6-546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 125123N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini152 – 383232Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini420 – 45536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini449 – 48436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RASGRP family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 N-terminal Ras-GEF domain.CuratedPROSITE-ProRule annotation
    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.CuratedPROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri494 – 54451Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG256085.
    HOGENOMiHOG000293171.
    HOVERGENiHBG007513.
    InParanoidiQ8IV61.
    KOiK12362.
    OMAiISFTDYQ.
    OrthoDBiEOG72G16V.
    PhylomeDBiQ8IV61.
    TreeFamiTF312918.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.840.10. 1 hit.
    InterProiIPR020454. DAG/PE-bd.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF13202. EF-hand_5. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view]
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 1 hit.
    SM00054. EFh. 2 hits.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48366. SSF48366. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8IV61-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE    50
    LAEKLLCMYR NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE 100
    EFREVASQLG YEKHVSLIDI SSIPSYDWMR RVTQRKKVSK KGKACLLFDH 150
    LEPIELAEHL TFLEHKSFRR ISFTDYQSYV IHGCLENNPT LERSIALFNG 200
    ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT LMAVVGGLSH 250
    SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP 300
    ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL 350
    EPNMDLINLL TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV 400
    PLEWALGVMP KPDPTVINKH IRKLVESVFR NYDHDHDGYI SQEDFESIAA 450
    NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR AKSQLHCKMG PGFIHNFQEM 500
    TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL VLACRRFARA 550
    PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK 600
    ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF 650
    AKWENEKPRV HAGVDVVDRG TEFELDQDEG EETRQDGEDG 690
    Length:690
    Mass (Da):78,332
    Last modified:March 1, 2003 - v1
    Checksum:i747BBAA2CCAC2D12
    GO
    Isoform 2 (identifier: Q8IV61-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         388-388: Missing.

    Show »
    Length:689
    Mass (Da):78,203
    Checksum:iF7A5C6385E3E2530
    GO

    Sequence cautioni

    The sequence BAA74869.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti393 – 3931T → A.
    Corresponds to variant rs13388394 [ dbSNP | Ensembl ].
    VAR_051901

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei388 – 3881Missing in isoform 2. 1 PublicationVSP_047371

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020653 mRNA. Translation: BAA74869.2. Different initiation.
    BX647990 mRNA. No translation available.
    AC020594 Genomic DNA. Translation: AAY15037.1.
    CH471053 Genomic DNA. Translation: EAX00432.1.
    CH471053 Genomic DNA. Translation: EAX00433.1.
    CH471053 Genomic DNA. Translation: EAX00434.1.
    BC027849 mRNA. Translation: AAH27849.1.
    CCDSiCCDS46256.1. [Q8IV61-1]
    CCDS54346.1. [Q8IV61-2]
    RefSeqiNP_001132960.1. NM_001139488.1. [Q8IV61-1]
    NP_056191.1. NM_015376.2. [Q8IV61-2]
    NP_733772.1. NM_170672.2. [Q8IV61-1]
    XP_005264303.1. XM_005264246.1. [Q8IV61-2]
    UniGeneiHs.143674.

    Genome annotation databases

    EnsembliENST00000402538; ENSP00000385886; ENSG00000152689. [Q8IV61-1]
    ENST00000403687; ENSP00000384192; ENSG00000152689. [Q8IV61-1]
    ENST00000407811; ENSP00000383917; ENSG00000152689. [Q8IV61-2]
    GeneIDi25780.
    KEGGihsa:25780.
    UCSCiuc002rox.3. human. [Q8IV61-1]
    uc002roy.3. human.

    Polymorphism databases

    DMDMi34395670.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020653 mRNA. Translation: BAA74869.2 . Different initiation.
    BX647990 mRNA. No translation available.
    AC020594 Genomic DNA. Translation: AAY15037.1 .
    CH471053 Genomic DNA. Translation: EAX00432.1 .
    CH471053 Genomic DNA. Translation: EAX00433.1 .
    CH471053 Genomic DNA. Translation: EAX00434.1 .
    BC027849 mRNA. Translation: AAH27849.1 .
    CCDSi CCDS46256.1. [Q8IV61-1 ]
    CCDS54346.1. [Q8IV61-2 ]
    RefSeqi NP_001132960.1. NM_001139488.1. [Q8IV61-1 ]
    NP_056191.1. NM_015376.2. [Q8IV61-2 ]
    NP_733772.1. NM_170672.2. [Q8IV61-1 ]
    XP_005264303.1. XM_005264246.1. [Q8IV61-2 ]
    UniGenei Hs.143674.

    3D structure databases

    ProteinModelPortali Q8IV61.
    SMRi Q8IV61. Positions 6-546.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117315. 10 interactions.
    IntActi Q8IV61. 2 interactions.
    STRINGi 9606.ENSP00000384192.

    Chemistry

    BindingDBi Q8IV61.
    ChEMBLi CHEMBL3638.

    PTM databases

    PhosphoSitei Q8IV61.

    Polymorphism databases

    DMDMi 34395670.

    Proteomic databases

    PaxDbi Q8IV61.
    PRIDEi Q8IV61.

    Protocols and materials databases

    DNASUi 25780.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000402538 ; ENSP00000385886 ; ENSG00000152689 . [Q8IV61-1 ]
    ENST00000403687 ; ENSP00000384192 ; ENSG00000152689 . [Q8IV61-1 ]
    ENST00000407811 ; ENSP00000383917 ; ENSG00000152689 . [Q8IV61-2 ]
    GeneIDi 25780.
    KEGGi hsa:25780.
    UCSCi uc002rox.3. human. [Q8IV61-1 ]
    uc002roy.3. human.

    Organism-specific databases

    CTDi 25780.
    GeneCardsi GC02P033661.
    H-InvDB HIX0001961.
    HGNCi HGNC:14545. RASGRP3.
    MIMi 609531. gene.
    neXtProti NX_Q8IV61.
    PharmGKBi PA134937092.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG256085.
    HOGENOMi HOG000293171.
    HOVERGENi HBG007513.
    InParanoidi Q8IV61.
    KOi K12362.
    OMAi ISFTDYQ.
    OrthoDBi EOG72G16V.
    PhylomeDBi Q8IV61.
    TreeFami TF312918.

    Enzyme and pathway databases

    Reactomei REACT_118778. Activation of RAS in B cells.

    Miscellaneous databases

    GeneWikii RASGRP3.
    GenomeRNAii 25780.
    NextBioi 46931.
    PROi Q8IV61.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8IV61.
    Bgeei Q8IV61.
    CleanExi HS_RASGRP3.
    Genevestigatori Q8IV61.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.840.10. 1 hit.
    InterProi IPR020454. DAG/PE-bd.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF13202. EF-hand_5. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 1 hit.
    SM00054. EFh. 2 hits.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48366. SSF48366. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain1 Publication.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Endometrium.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction."
      Rebhun J.F., Castro A.F., Quilliam L.A.
      J. Biol. Chem. 275:34901-34908(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGRP3_HUMAN
    AccessioniPrimary (citable) accession number: Q8IV61
    Secondary accession number(s): D6W583, O94931, Q53SD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3