Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8IV61 (GRP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras guanyl-releasing protein 3
Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor III
Guanine nucleotide exchange factor for Rap1
Gene names
Name:RASGRP3
Synonyms:GRP3, KIAA0846
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Guanine nucleotide exchange factor (GEF) for Ras and Rap1. Ref.6

Sequence similarities

Belongs to the RASGRP family.

Contains 2 EF-hand domains.

Contains 1 N-terminal Ras-GEF domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 Ras-GEF domain.

Sequence caution

The sequence BAA74869.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandCalcium
Metal-binding
Zinc
   Molecular functionGuanine-nucleotide releasing factor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Non-traceable author statement Ref.6. Source: UniProtKB

Ras protein signal transduction

Inferred from electronic annotation. Source: Ensembl

positive regulation of Rap GTPase activity

Inferred from direct assay Ref.6. Source: GOC

small GTPase mediated signal transduction

Traceable author statement Ref.6. Source: UniProtKB

   Cellular_componentguanyl-nucleotide exchange factor complex

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Non-traceable author statement Ref.6. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionRap GTPase activator activity

Inferred from direct assay Ref.6. Source: UniProtKB

Ras guanyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: Ensembl

calcium ion binding

Non-traceable author statement Ref.6. Source: UniProtKB

diacylglycerol binding

Non-traceable author statement Ref.6. Source: UniProtKB

guanyl-nucleotide exchange factor activity

Traceable author statement. Source: Reactome

signal transducer activity

Traceable author statement Ref.6. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IV61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IV61-2)

The sequence of this isoform differs from the canonical sequence as follows:
     388-388: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 690690Ras guanyl-releasing protein 3
PRO_0000068883

Regions

Domain3 – 125123N-terminal Ras-GEF
Domain152 – 383232Ras-GEF
Domain420 – 45536EF-hand 1
Domain449 – 48436EF-hand 2
Calcium binding433 – 444121 Potential
Calcium binding462 – 473122 Potential
Zinc finger494 – 54451Phorbol-ester/DAG-type

Natural variations

Alternative sequence3881Missing in isoform 2.
VSP_047371
Natural variant3931T → A.
Corresponds to variant rs13388394 [ dbSNP | Ensembl ].
VAR_051901

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 747BBAA2CCAC2D12

FASTA69078,332
        10         20         30         40         50         60 
MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR 

        70         80         90        100        110        120 
NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI 

       130        140        150        160        170        180 
SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV 

       190        200        210        220        230        240 
IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT 

       250        260        270        280        290        300 
LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP 

       310        320        330        340        350        360 
ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL 

       370        380        390        400        410        420 
TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH 

       430        440        450        460        470        480 
IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR 

       490        500        510        520        530        540 
AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL 

       550        560        570        580        590        600 
VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK 

       610        620        630        640        650        660 
ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV 

       670        680        690 
HAGVDVVDRG TEFELDQDEG EETRQDGEDG 

« Hide

Isoform 2 [UniParc].

Checksum: F7A5C6385E3E2530
Show »

FASTA68978,203

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Endometrium.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction."
Rebhun J.F., Castro A.F., Quilliam L.A.
J. Biol. Chem. 275:34901-34908(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020653 mRNA. Translation: BAA74869.2. Different initiation.
BX647990 mRNA. No translation available.
AC020594 Genomic DNA. Translation: AAY15037.1.
CH471053 Genomic DNA. Translation: EAX00432.1.
CH471053 Genomic DNA. Translation: EAX00433.1.
CH471053 Genomic DNA. Translation: EAX00434.1.
BC027849 mRNA. Translation: AAH27849.1.
RefSeqNP_001132960.1. NM_001139488.1.
NP_056191.1. NM_015376.2.
NP_733772.1. NM_170672.2.
XP_005264303.1. XM_005264246.1.
UniGeneHs.143674.

3D structure databases

ProteinModelPortalQ8IV61.
SMRQ8IV61. Positions 6-666.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117315. 10 interactions.
IntActQ8IV61. 2 interactions.
STRING9606.ENSP00000384192.

Chemistry

BindingDBQ8IV61.
ChEMBLCHEMBL3638.

PTM databases

PhosphoSiteQ8IV61.

Polymorphism databases

DMDM34395670.

Proteomic databases

PaxDbQ8IV61.
PRIDEQ8IV61.

Protocols and materials databases

DNASU25780.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000402538; ENSP00000385886; ENSG00000152689. [Q8IV61-1]
ENST00000403687; ENSP00000384192; ENSG00000152689. [Q8IV61-1]
ENST00000407811; ENSP00000383917; ENSG00000152689. [Q8IV61-2]
GeneID25780.
KEGGhsa:25780.
UCSCuc002rox.3. human. [Q8IV61-1]

Organism-specific databases

CTD25780.
GeneCardsGC02P033661.
H-InvDBHIX0001961.
HGNCHGNC:14545. RASGRP3.
MIM609531. gene.
neXtProtNX_Q8IV61.
PharmGKBPA134937092.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG256085.
HOGENOMHOG000293171.
HOVERGENHBG007513.
InParanoidQ8IV61.
KOK12362.
OMAISFTDYQ.
OrthoDBEOG72G16V.
PhylomeDBQ8IV61.
TreeFamTF312918.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ8IV61.
BgeeQ8IV61.
CleanExHS_RASGRP3.
GenevestigatorQ8IV61.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.840.10. 1 hit.
InterProIPR020454. DAG/PE-bd.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR008937. Ras_GEF.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view]
PANTHERPTHR23113. PTHR23113. 1 hit.
PfamPF00130. C1_1. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 1 hit.
SM00054. EFh. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMSSF48366. SSF48366. 1 hit.
PROSITEPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRASGRP3.
GenomeRNAi25780.
NextBio46931.
PROQ8IV61.
SOURCESearch...

Entry information

Entry nameGRP3_HUMAN
AccessionPrimary (citable) accession number: Q8IV61
Secondary accession number(s): D6W583, O94931, Q53SD7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM