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Q8IV61

- GRP3_HUMAN

UniProt

Q8IV61 - GRP3_HUMAN

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Protein

Ras guanyl-releasing protein 3

Gene
RASGRP3, GRP3, KIAA0846
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF) for Ras and Rap1.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi433 – 444121 Reviewed predictionAdd
BLAST
Calcium bindingi462 – 473122 Reviewed predictionAdd
BLAST
Zinc fingeri494 – 54451Phorbol-ester/DAG-typeAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. diacylglycerol binding Source: UniProtKB
  3. guanyl-nucleotide exchange factor activity Source: Reactome
  4. Rap GTPase activator activity Source: UniProtKB
  5. Ras guanyl-nucleotide exchange factor activity Source: Ensembl
  6. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. MAPK cascade Source: UniProtKB
  2. positive regulation of Rap GTPase activity Source: GOC
  3. Ras protein signal transduction Source: Ensembl
  4. small GTPase mediated signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_118778. Activation of RAS in B cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras guanyl-releasing protein 3
Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor III
Guanine nucleotide exchange factor for Rap1
Gene namesi
Name:RASGRP3
Synonyms:GRP3, KIAA0846
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:14545. RASGRP3.

Subcellular locationi

GO - Cellular componenti

  1. guanyl-nucleotide exchange factor complex Source: Ensembl
  2. integral component of plasma membrane Source: UniProtKB
  3. perinuclear region of cytoplasm Source: Ensembl
  4. plasma membrane Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134937092.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Ras guanyl-releasing protein 3PRO_0000068883Add
BLAST

Proteomic databases

PaxDbiQ8IV61.
PRIDEiQ8IV61.

PTM databases

PhosphoSiteiQ8IV61.

Expressioni

Gene expression databases

ArrayExpressiQ8IV61.
BgeeiQ8IV61.
CleanExiHS_RASGRP3.
GenevestigatoriQ8IV61.

Interactioni

Protein-protein interaction databases

BioGridi117315. 10 interactions.
IntActiQ8IV61. 2 interactions.
STRINGi9606.ENSP00000384192.

Structurei

3D structure databases

ProteinModelPortaliQ8IV61.
SMRiQ8IV61. Positions 6-546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 125123N-terminal Ras-GEFAdd
BLAST
Domaini152 – 383232Ras-GEFAdd
BLAST
Domaini420 – 45536EF-hand 1Add
BLAST
Domaini449 – 48436EF-hand 2Add
BLAST

Sequence similaritiesi

Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 Ras-GEF domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG256085.
HOGENOMiHOG000293171.
HOVERGENiHBG007513.
InParanoidiQ8IV61.
KOiK12362.
OMAiISFTDYQ.
OrthoDBiEOG72G16V.
PhylomeDBiQ8IV61.
TreeFamiTF312918.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.840.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00054. EFh. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8IV61-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE    50
LAEKLLCMYR NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE 100
EFREVASQLG YEKHVSLIDI SSIPSYDWMR RVTQRKKVSK KGKACLLFDH 150
LEPIELAEHL TFLEHKSFRR ISFTDYQSYV IHGCLENNPT LERSIALFNG 200
ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT LMAVVGGLSH 250
SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP 300
ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL 350
EPNMDLINLL TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV 400
PLEWALGVMP KPDPTVINKH IRKLVESVFR NYDHDHDGYI SQEDFESIAA 450
NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR AKSQLHCKMG PGFIHNFQEM 500
TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL VLACRRFARA 550
PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK 600
ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF 650
AKWENEKPRV HAGVDVVDRG TEFELDQDEG EETRQDGEDG 690
Length:690
Mass (Da):78,332
Last modified:March 1, 2003 - v1
Checksum:i747BBAA2CCAC2D12
GO
Isoform 2 (identifier: Q8IV61-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-388: Missing.

Show »
Length:689
Mass (Da):78,203
Checksum:iF7A5C6385E3E2530
GO

Sequence cautioni

The sequence BAA74869.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti393 – 3931T → A.
Corresponds to variant rs13388394 [ dbSNP | Ensembl ].
VAR_051901

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei388 – 3881Missing in isoform 2. VSP_047371

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020653 mRNA. Translation: BAA74869.2. Different initiation.
BX647990 mRNA. No translation available.
AC020594 Genomic DNA. Translation: AAY15037.1.
CH471053 Genomic DNA. Translation: EAX00432.1.
CH471053 Genomic DNA. Translation: EAX00433.1.
CH471053 Genomic DNA. Translation: EAX00434.1.
BC027849 mRNA. Translation: AAH27849.1.
CCDSiCCDS46256.1. [Q8IV61-1]
CCDS54346.1. [Q8IV61-2]
RefSeqiNP_001132960.1. NM_001139488.1. [Q8IV61-1]
NP_056191.1. NM_015376.2. [Q8IV61-2]
NP_733772.1. NM_170672.2. [Q8IV61-1]
XP_005264303.1. XM_005264246.1. [Q8IV61-2]
UniGeneiHs.143674.

Genome annotation databases

EnsembliENST00000402538; ENSP00000385886; ENSG00000152689. [Q8IV61-1]
ENST00000403687; ENSP00000384192; ENSG00000152689. [Q8IV61-1]
ENST00000407811; ENSP00000383917; ENSG00000152689. [Q8IV61-2]
GeneIDi25780.
KEGGihsa:25780.
UCSCiuc002rox.3. human. [Q8IV61-1]
uc002roy.3. human.

Polymorphism databases

DMDMi34395670.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020653 mRNA. Translation: BAA74869.2 . Different initiation.
BX647990 mRNA. No translation available.
AC020594 Genomic DNA. Translation: AAY15037.1 .
CH471053 Genomic DNA. Translation: EAX00432.1 .
CH471053 Genomic DNA. Translation: EAX00433.1 .
CH471053 Genomic DNA. Translation: EAX00434.1 .
BC027849 mRNA. Translation: AAH27849.1 .
CCDSi CCDS46256.1. [Q8IV61-1 ]
CCDS54346.1. [Q8IV61-2 ]
RefSeqi NP_001132960.1. NM_001139488.1. [Q8IV61-1 ]
NP_056191.1. NM_015376.2. [Q8IV61-2 ]
NP_733772.1. NM_170672.2. [Q8IV61-1 ]
XP_005264303.1. XM_005264246.1. [Q8IV61-2 ]
UniGenei Hs.143674.

3D structure databases

ProteinModelPortali Q8IV61.
SMRi Q8IV61. Positions 6-546.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117315. 10 interactions.
IntActi Q8IV61. 2 interactions.
STRINGi 9606.ENSP00000384192.

Chemistry

BindingDBi Q8IV61.
ChEMBLi CHEMBL3638.

PTM databases

PhosphoSitei Q8IV61.

Polymorphism databases

DMDMi 34395670.

Proteomic databases

PaxDbi Q8IV61.
PRIDEi Q8IV61.

Protocols and materials databases

DNASUi 25780.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000402538 ; ENSP00000385886 ; ENSG00000152689 . [Q8IV61-1 ]
ENST00000403687 ; ENSP00000384192 ; ENSG00000152689 . [Q8IV61-1 ]
ENST00000407811 ; ENSP00000383917 ; ENSG00000152689 . [Q8IV61-2 ]
GeneIDi 25780.
KEGGi hsa:25780.
UCSCi uc002rox.3. human. [Q8IV61-1 ]
uc002roy.3. human.

Organism-specific databases

CTDi 25780.
GeneCardsi GC02P033661.
H-InvDB HIX0001961.
HGNCi HGNC:14545. RASGRP3.
MIMi 609531. gene.
neXtProti NX_Q8IV61.
PharmGKBi PA134937092.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG256085.
HOGENOMi HOG000293171.
HOVERGENi HBG007513.
InParanoidi Q8IV61.
KOi K12362.
OMAi ISFTDYQ.
OrthoDBi EOG72G16V.
PhylomeDBi Q8IV61.
TreeFami TF312918.

Enzyme and pathway databases

Reactomei REACT_118778. Activation of RAS in B cells.

Miscellaneous databases

GeneWikii RASGRP3.
GenomeRNAii 25780.
NextBioi 46931.
PROi Q8IV61.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8IV61.
Bgeei Q8IV61.
CleanExi HS_RASGRP3.
Genevestigatori Q8IV61.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.840.10. 1 hit.
InterProi IPR020454. DAG/PE-bd.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view ]
PRINTSi PR00008. DAGPEDOMAIN.
SMARTi SM00109. C1. 1 hit.
SM00054. EFh. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 1 hit.
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Endometrium.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction."
    Rebhun J.F., Castro A.F., Quilliam L.A.
    J. Biol. Chem. 275:34901-34908(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGRP3_HUMAN
AccessioniPrimary (citable) accession number: Q8IV61
Secondary accession number(s): D6W583, O94931, Q53SD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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