Reviewed, UniProtKB/Swiss-Prot Q8IV61 (GRP3_HUMAN)

Names and origin

Protein names

Recommended name:
    Ras guanyl-releasing protein 3
Alternative name(s):
    Calcium and DAG-regulated guanine nucleotide exchange factor III
    Guanine nucleotide exchange factor for Rap1

Gene names

Name:	RASGRP3
Synonyms:	GRP3, KIAA0846

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) for Ras and Rap1. Ref.3
Sequence similarities	Belongs to the RASGRP family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	Calcium Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
Gene Ontology (GO)
Biological process	MAPKKK cascade Ref.3 Non-traceable author statement. Source: UniProtKB regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Ref.3 Non-traceable author statement. Source: UniProtKB
Molecular function	Rap GTPase activator activity Ref.3 Inferred from direct assay. Source: UniProtKB calcium ion binding Ref.3 Non-traceable author statement. Source: UniProtKB diacylglycerol binding Ref.3 Non-traceable author statement. Source: UniProtKB signal transducer activity Ref.3 Traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAQ	P27348	1	EBI-1047876,EBI-359854

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Ras guanyl-releasing protein 3		PRO_0000068883
Regions
	Domain	3 – 125	123	N-terminal Ras-GEF
	Domain	152 – 383	232	Ras-GEF
	Domain	420 – 455	36	EF-hand 1
	Domain	449 – 484	36	EF-hand 2
	Calcium binding	433 – 444	12	1 Potential
	Calcium binding	462 – 473	12	2 Potential
	Zinc finger	494 – 544	51	Phorbol-ester/DAG-type
Natural variations
	Natural variant	393	1	T → A: dbSNP rs13388394.		VAR_051901
Experimental info
	Sequence conflict	388	1	Missing in BAA74869. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IV61-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 747BBAA2CCAC2D12	FASTA	690	78,332
	10 20 30 40 50 60 MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR 70 80 90 100 110 120 NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI 130 140 150 160 170 180 SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV 190 200 210 220 230 240 IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT 250 260 270 280 290 300 LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP 310 320 330 340 350 360 ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL 370 380 390 400 410 420 TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH 430 440 450 460 470 480 IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR 490 500 510 520 530 540 AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL 550 560 570 580 590 600 VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK 610 620 630 640 650 660 ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV 670 680 690 HAGVDVVDRG TEFELDQDEG EETRQDGEDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction." Rebhun J.F., Castro A.F., Quilliam L.A. J. Biol. Chem. 275:34901-34908(2000) [PubMed: 10934204] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB020653 mRNA. Translation: BAA74869.2. Different initiation. BC027849 mRNA. Translation: AAH27849.1.
IPI	IPI00171874.
RefSeq	NP_001132960.1. NP_733772.1.
UniGene	Hs.143674
3D structure databases
HSSP	HSSP built from PDB template 1PTQ based on UniProtKB P28867.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IV61. 1 interaction.
PTM databases
PhosphoSite	Q8IV61.
Proteomic databases
PRIDE	Q8IV61.
Genome annotation databases
Ensembl	ENSG00000152689. Homo sapiens. [Contig view]
GeneID	25780.
KEGG	hsa:25780.
Organism-specific databases
GeneCards	GC02P033573.
H-InvDB	HIX0001961.
HGNC	HGNC:14545. RASGRP3.
MIM	609531. gene.
PharmGKB	PA134937092.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8IV61.
HOVERGEN	Q8IV61.
OMA	Q8IV61. FRRISFT.
Gene expression databases
ArrayExpress	Q8IV61.
Bgee	Q8IV61.
CleanEx	HS_RASGRP3.
Family and domain databases
InterPro	IPR002219. DAG_PE_bd. IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR008937. Ras_GEF. IPR000651. RasGef_N. IPR001895. RasGRF_CDC25. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:1.10.840.10. RasGRF_CDC25. 1 hit.
PANTHER	PTHR23113. Ras_GEF. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00036. efhand. 1 hit. PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. [Graphical view]
SMART	SM00109. C1. 1 hit. SM00054. EFh. 2 hits. SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	46931.
SOURCE	Search...

Entry information

Entry name

GRP3_HUMAN

Accession

Primary (citable) accession number: Q8IV61
Secondary accession number(s): O94931

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)