Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-seryl-tRNA(Sec) kinase

Gene

PSTK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.By similarity

Catalytic activityi

ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec).

Cofactori

Mg2+By similarity

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (PSTK)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase, O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS), O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2408557. Selenocysteine synthesis.
UniPathwayiUPA00906; UER00897.

Names & Taxonomyi

Protein namesi
Recommended name:
L-seryl-tRNA(Sec) kinase (EC:2.7.1.164)
Alternative name(s):
O-phosphoseryl-tRNA(Sec) kinase
Gene namesi
Name:PSTK
Synonyms:C10orf89
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:28578. PSTK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162400268.

Polymorphism and mutation databases

BioMutaiPSTK.
DMDMi116242735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348L-seryl-tRNA(Sec) kinasePRO_0000097080Add
BLAST

Proteomic databases

PaxDbiQ8IV42.
PRIDEiQ8IV42.

PTM databases

iPTMnetiQ8IV42.
PhosphoSiteiQ8IV42.

Expressioni

Gene expression databases

BgeeiQ8IV42.
CleanExiHS_PSTK.
ExpressionAtlasiQ8IV42. baseline and differential.
GenevisibleiQ8IV42. HS.

Organism-specific databases

HPAiHPA037781.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000357882.

Structurei

3D structure databases

ProteinModelPortaliQ8IV42.
SMRiQ8IV42. Positions 20-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-seryl-tRNA(Sec) kinase family.Curated

Phylogenomic databases

eggNOGiKOG4622. Eukaryota.
ENOG4111HI0. LUCA.
GeneTreeiENSGT00390000017554.
HOGENOMiHOG000115749.
HOVERGENiHBG064780.
InParanoidiQ8IV42.
KOiK10837.
OrthoDBiEOG7G1V7T.
PhylomeDBiQ8IV42.
TreeFamiTF321264.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020028. L-seryl-tRNA_Sec_kinase_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR03575. selen_PSTK_euk. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IV42-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI
60 70 80 90 100
GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM
110 120 130 140 150
SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL
160 170 180 190 200
DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE
210 220 230 240 250
TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP
260 270 280 290 300
VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE
310 320 330 340
MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG
Length:348
Mass (Da):39,527
Last modified:October 17, 2006 - v2
Checksum:iA7DDFF0BDC93BE4E
GO
Isoform 2 (identifier: Q8IV42-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     262-348: DTDRIICSTN...RIEKRPLSTG → VNFQCKFNVNEKEQLFVGRSFEPIIKCE

Show »
Length:289
Mass (Da):32,749
Checksum:i2DB837E8522CF6E6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti206 – 2061G → R.1 Publication
Corresponds to variant rs3736582 [ dbSNP | Ensembl ].
VAR_028156

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei262 – 34887DTDRI…PLSTG → VNFQCKFNVNEKEQLFVGRS FEPIIKCE in isoform 2. 1 PublicationVSP_014989Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK127173 mRNA. Translation: BAC86867.1.
BC035344 mRNA. Translation: AAH35344.1.
CCDSiCCDS7633.1. [Q8IV42-1]
RefSeqiNP_699167.2. NM_153336.2. [Q8IV42-1]
UniGeneiHs.281004.

Genome annotation databases

EnsembliENST00000368887; ENSP00000357882; ENSG00000179988. [Q8IV42-1]
ENST00000405485; ENSP00000384764; ENSG00000179988. [Q8IV42-1]
GeneIDi118672.
KEGGihsa:118672.
UCSCiuc001lgy.2. human. [Q8IV42-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK127173 mRNA. Translation: BAC86867.1.
BC035344 mRNA. Translation: AAH35344.1.
CCDSiCCDS7633.1. [Q8IV42-1]
RefSeqiNP_699167.2. NM_153336.2. [Q8IV42-1]
UniGeneiHs.281004.

3D structure databases

ProteinModelPortaliQ8IV42.
SMRiQ8IV42. Positions 20-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000357882.

PTM databases

iPTMnetiQ8IV42.
PhosphoSiteiQ8IV42.

Polymorphism and mutation databases

BioMutaiPSTK.
DMDMi116242735.

Proteomic databases

PaxDbiQ8IV42.
PRIDEiQ8IV42.

Protocols and materials databases

DNASUi118672.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368887; ENSP00000357882; ENSG00000179988. [Q8IV42-1]
ENST00000405485; ENSP00000384764; ENSG00000179988. [Q8IV42-1]
GeneIDi118672.
KEGGihsa:118672.
UCSCiuc001lgy.2. human. [Q8IV42-1]

Organism-specific databases

CTDi118672.
GeneCardsiPSTK.
HGNCiHGNC:28578. PSTK.
HPAiHPA037781.
MIMi611310. gene.
neXtProtiNX_Q8IV42.
PharmGKBiPA162400268.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4622. Eukaryota.
ENOG4111HI0. LUCA.
GeneTreeiENSGT00390000017554.
HOGENOMiHOG000115749.
HOVERGENiHBG064780.
InParanoidiQ8IV42.
KOiK10837.
OrthoDBiEOG7G1V7T.
PhylomeDBiQ8IV42.
TreeFamiTF321264.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00897.
ReactomeiR-HSA-2408557. Selenocysteine synthesis.

Miscellaneous databases

ChiTaRSiPSTK. human.
GenomeRNAii118672.
PROiQ8IV42.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IV42.
CleanExiHS_PSTK.
ExpressionAtlasiQ8IV42. baseline and differential.
GenevisibleiQ8IV42. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020028. L-seryl-tRNA_Sec_kinase_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR03575. selen_PSTK_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Corpus callosum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-206.
    Tissue: Colon.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSTK_HUMAN
AccessioniPrimary (citable) accession number: Q8IV42
Secondary accession number(s): Q6ZSS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 17, 2006
Last modified: June 8, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.