Q8IV08 (PLD3_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 89.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phospholipase D3 Short name=PLD 3 EC=3.1.4.4 Alternative name(s): Choline phosphatase 3 HindIII K4L homolog Hu-K4 Phosphatidylcholine-hydrolyzing phospholipase D3 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Reference proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 490 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | A phosphatidylcholine + H2O = choline + a phosphatidate. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.3. |
| Tissue specificity | Widely expressed. Expressed at higher level in brain. Expressed at low level in corpus callosum, suggesting that it is highly expressed in neurons. Ref.3 |
| Post-translational modification | Glycosylated. Ref.3 |
| Sequence similarities | Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains. |
| Sequence caution | The sequence AAB16799.1 differs from that shown. Reason: Frameshift at position 52. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid degradation Lipid metabolism |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Repeat Signal-anchor Transmembrane Transmembrane helix |
| Molecular function | Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylglycerol biosynthetic processTraceable author statement. Source: Reactome small molecule metabolic processTraceable author statement. Source: Reactome |
| Cellular_component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activityTraceable author statement Ref.1. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| NFKB1 | P19838 | 2 | EBI-2689908,EBI-300010 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 490 | 490 | Phospholipase D3 | PRO_0000280326 | |||||
Regions | |||||||||
| Topological domain | 1 – 38 | 38 | Cytoplasmic Potential | ||||||
| Transmembrane | 39 – 59 | 21 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 60 – 490 | 431 | Lumenal Potential | ||||||
| Domain | 196 – 223 | 28 | PLD phosphodiesterase 1 | ||||||
| Domain | 411 – 437 | 27 | PLD phosphodiesterase 2 | ||||||
Sites | |||||||||
| Active site | 201 | 1 | Potential | ||||||
| Active site | 203 | 1 | Potential | ||||||
| Active site | 208 | 1 | Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 97 | 1 | N-linked (GlcNAc...) Ref.4 | ||||||
| Glycosylation | 132 | 1 | N-linked (GlcNAc...) Ref.4 | ||||||
Experimental info | |||||||||
| Sequence conflict | 473 | 1 | S → I in AAB16799. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A human homolog of the vaccinia virus HindIII K4L gene is a member of the phospholipase D superfamily." Cao J.X., Koop B.F., Upton C. Virus Res. 48:11-18(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Colon and Lung. |
| [3] | "Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum." Munck A., Boehm C., Seibel N.M., Hashemol Hosseini Z., Hampe W. FEBS J. 272:1718-1726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY, TISSUE SPECIFICITY, GLYCOSYLATION. |
| [4] | "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-97 AND ASN-132, MASS SPECTROMETRY. Tissue: Liver. |
| [5] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U60644 mRNA. Translation: AAB16799.1. Frameshift. BC000553 mRNA. Translation: AAH00553.2. BC036327 mRNA. Translation: AAH36327.1. BC096820 mRNA. Translation: AAH96820.1. |
| IPI | IPI00328243. |
| RefSeq | NP_001026866.1. NM_001031696.2. NP_036400.2. NM_012268.2. |
| UniGene | Hs.257008. |
3D structure databases | |
| ProteinModelPortal | Q8IV08. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q8IV08. 2 interactions. |
| STRING | 9606.ENSP00000348901. |
PTM databases | |
| PhosphoSite | Q8IV08. |
Polymorphism databases | |
| DMDM | 74750647. |
Proteomic databases | |
| PaxDb | Q8IV08. |
| PRIDE | Q8IV08. |
Protocols and materials databases | |
| DNASU | 23646. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000356508; ENSP00000348901; ENSG00000105223. ENST00000409281; ENSP00000387022; ENSG00000105223. ENST00000409419; ENSP00000386293; ENSG00000105223. ENST00000409587; ENSP00000387050; ENSG00000105223. ENST00000409735; ENSP00000386938; ENSG00000105223. |
| GeneID | 23646. |
| KEGG | hsa:23646. |
| UCSC | uc002onj.4. human. |
Organism-specific databases | |
| CTD | 23646. |
| GeneCards | GC19P040854. |
| HGNC | HGNC:17158. PLD3. |
| HPA | CAB020812. HPA012800. |
| neXtProt | NX_Q8IV08. |
| PharmGKB | PA134887482. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | NOG70733. |
| HOGENOM | HOG000293407. |
| HOVERGEN | HBG052880. |
| InParanoid | Q8IV08. |
| KO | K16860. |
| OMA | GKIFEAY. |
| PhylomeDB | Q8IV08. |
Enzyme and pathway databases | |
| Reactome | REACT_111217. Metabolism. |
Gene expression databases | |
| ArrayExpress | Q8IV08. |
| Bgee | Q8IV08. |
| CleanEx | HS_PLD3. |
| Genevestigator | Q8IV08. |
Family and domain databases | |
| InterPro | IPR001736. PLipase_D/transphosphatidylase. [Graphical view] |
| SMART | SM00155. PLDc. 2 hits. [Graphical view] |
| PROSITE | PS50035. PLD. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| BindingDB | Q8IV08. |
| ChEMBL | CHEMBL2769. |
| ChiTaRS | PLD3. human. |
| GenomeRNAi | 23646. |
| NextBio | 46461. |
Entry information
| Entry name | PLD3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8IV08 Secondary accession number(s): Q92853, Q9BW87 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| SIMILARITY comments Index of protein domains and families |

Clusters with
