Reviewed, UniProtKB/Swiss-Prot Q8IV08 (PLD3_HUMAN)

Names and origin

Protein names

Recommended name:
    Phospholipase D3
      Short name=PLD 3
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 3
    Phosphatidylcholine-hydrolyzing phospholipase D3
    HindIII K4L homolog
    Hu-K4

Gene names

Name:

PLD3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Ref.3
Tissue specificity	Widely expressed. Expressec at higher level in brain. Expresse at low level in corpus callosum, suggsting that it is highly expressed in neurons. Ref.3
Post-translational modification	Glycosylated. Ref.3 Ref.5
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.
Sequence caution	The sequence AAB16799.1 differs from that shown. Reason: Frameshift at position 52.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Endoplasmic reticulum Membrane
Domain	Repeat Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Phospholipase D3		PRO_0000280326
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	Signal-anchor for type II membrane protein Potential
	Topological domain	60 – 490	431	Lumenal Potential
	Domain	196 – 223	28	PLD phosphodiesterase 1
	Domain	411 – 437	27	PLD phosphodiesterase 2
Sites
	Active site	201	1	Potential
	Active site	203	1	Potential
	Active site	208	1	Potential
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	132	1	N-linked (GlcNAc...) Ref.5
Experimental info
	Sequence conflict	473	1	S → I in AAB16799. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IV08-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 444EC4D02F5610F1	FASTA	490	54,705
	10 20 30 40 50 60 MKPKLMYQEL KVPAEEPANE LPMNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW 70 80 90 100 110 120 EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLDFPNAST GNPSTSQAWL GLLAGAHSSL 130 140 150 160 170 180 DIASFYWTLT NNDTHTQEPS AQQGEEVLRQ LQTLAPKGVN VRIAVSKPSG PQPQADLQAL 190 200 210 220 230 240 LQSGAQVRMV DMQKLTHGVL HTKFWVVDQT HFYLGSANMD WRSLTQVKEL GVVMYNCSCL 250 260 270 280 290 300 ARDLTKIFEA YWFLGQAGSS IPSTWPRFYD TRYNQETPME ICLNGTPALA YLASAPPPLC 310 320 330 340 350 360 PSGRTPDLKA LLNVVDNARS FIYVAVMNYL PTLEFSHPHR FWPAIDDGLR RATYERGVKV 370 380 390 400 410 420 RLLISCWGHS EPSMRAFLLS LAALRDNHTH SDIQVKLFVV PADEAQARIP YARVNHNKYM 430 440 450 460 470 480 VTERATYIGT SNWSGNYFTE TAGTSLLVTQ NGRGGLRSQL EAIFLRDWDS PYSHDLDTSA 490 DSVGNACRLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human homolog of the vaccinia virus HindIII K4L gene is a member of the phospholipase D superfamily." Cao J.X., Koop B.F., Upton C. Virus Res. 48:11-18(1997) [PubMed: 9140189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Colon and Lung.
[3]	"Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum." Munck A., Boehm C., Seibel N.M., Hashemol Hosseini Z., Hampe W. FEBS J. 272:1718-1726(2005) [PubMed: 15794758] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY, TISSUE SPECIFICITY, GLYCOSYLATION.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-97 AND ASN-132, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U60644 mRNA. Translation: AAB16799.1. Frameshift. BC000553 mRNA. Translation: AAH00553.2. BC036327 mRNA. Translation: AAH36327.1. BC096820 mRNA. Translation: AAH96820.1.
IPI	IPI00328243.
RefSeq	NP_001026866.1. NP_036400.2.
UniGene	Hs.257008
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8IV08.
Proteomic databases
PRIDE	Q8IV08.
Genome annotation databases
Ensembl	ENST00000356508; ENSP00000348901; ENSG00000105223; Homo sapiens. [Genome view] ENST00000359274; ENSP00000352220; ENSG00000105223; Homo sapiens. [Genome view] ENST00000392032; ENSP00000375886; ENSG00000105223; Homo sapiens. [Genome view] ENST00000409281; ENSP00000387022; ENSG00000105223; Homo sapiens. [Genome view] ENST00000409419; ENSP00000386293; ENSG00000105223; Homo sapiens. [Genome view] ENST00000409587; ENSP00000387050; ENSG00000105223; Homo sapiens. [Genome view] ENST00000409735; ENSP00000386938; ENSG00000105223; Homo sapiens. [Genome view]
GeneID	23646.
KEGG	hsa:23646.
UCSC	uc002onj.2. human.
Organism-specific databases
CTD	23646.
GeneCards	GC19P045557.
HGNC	HGNC:17158. PLD3.
HPA	CAB020812. HPA012800.
PharmGKB	PA134887482.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8IV08.
OMA	VFERDWN.
Enzyme and pathway databases
BRENDA	3.1.4.4. 247.
Gene expression databases
ArrayExpress	Q8IV08.
Bgee	Q8IV08.
CleanEx	HS_PLD3.
Genevestigator	Q8IV08.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	46461.

Entry information

Entry name

PLD3_HUMAN

Accession

Primary (citable) accession number: Q8IV08
Secondary accession number(s): Q92853, Q9BW87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.