Q8IV08 (PLD3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D3
Short name=PLD 3
EC=3.1.4.4

Alternative name(s):
Choline phosphatase 3
HindIII K4L homolog
Hu-K4
Phosphatidylcholine-hydrolyzing phospholipase D3

Gene names

Name:

PLD3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.3.
Tissue specificity	Widely expressed. Expressed at higher level in brain. Expressed at low level in corpus callosum, suggesting that it is highly expressed in neurons. Ref.3
Post-translational modification	Glycosylated. Ref.3
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.
Sequence caution	The sequence AAB16799.1 differs from that shown. Reason: Frameshift at position 52.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Repeat Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylglycerol biosynthetic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NFKB1	P19838	2	EBI-2689908,EBI-300010

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Phospholipase D3		PRO_0000280326
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	60 – 490	431	Lumenal Potential
	Domain	196 – 223	28	PLD phosphodiesterase 1
	Domain	411 – 437	27	PLD phosphodiesterase 2
Sites
	Active site	201	1	Potential
	Active site	203	1	Potential
	Active site	208	1	Potential
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	132	1	N-linked (GlcNAc...) Ref.4
Experimental info
	Sequence conflict	473	1	S → I in AAB16799. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IV08 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 444EC4D02F5610F1	FASTA	490	54,705
	10 20 30 40 50 60 MKPKLMYQEL KVPAEEPANE LPMNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW 70 80 90 100 110 120 EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLDFPNAST GNPSTSQAWL GLLAGAHSSL 130 140 150 160 170 180 DIASFYWTLT NNDTHTQEPS AQQGEEVLRQ LQTLAPKGVN VRIAVSKPSG PQPQADLQAL 190 200 210 220 230 240 LQSGAQVRMV DMQKLTHGVL HTKFWVVDQT HFYLGSANMD WRSLTQVKEL GVVMYNCSCL 250 260 270 280 290 300 ARDLTKIFEA YWFLGQAGSS IPSTWPRFYD TRYNQETPME ICLNGTPALA YLASAPPPLC 310 320 330 340 350 360 PSGRTPDLKA LLNVVDNARS FIYVAVMNYL PTLEFSHPHR FWPAIDDGLR RATYERGVKV 370 380 390 400 410 420 RLLISCWGHS EPSMRAFLLS LAALRDNHTH SDIQVKLFVV PADEAQARIP YARVNHNKYM 430 440 450 460 470 480 VTERATYIGT SNWSGNYFTE TAGTSLLVTQ NGRGGLRSQL EAIFLRDWDS PYSHDLDTSA 490 DSVGNACRLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human homolog of the vaccinia virus HindIII K4L gene is a member of the phospholipase D superfamily." Cao J.X., Koop B.F., Upton C. Virus Res. 48:11-18(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Colon and Lung.
[3]	"Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum." Munck A., Boehm C., Seibel N.M., Hashemol Hosseini Z., Hampe W. FEBS J. 272:1718-1726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY, TISSUE SPECIFICITY, GLYCOSYLATION.
[4]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-97 AND ASN-132, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60644 mRNA. Translation: AAB16799.1. Frameshift. BC000553 mRNA. Translation: AAH00553.2. BC036327 mRNA. Translation: AAH36327.1. BC096820 mRNA. Translation: AAH96820.1.
IPI	IPI00328243.
RefSeq	NP_001026866.1. NM_001031696.2. NP_036400.2. NM_012268.2.
UniGene	Hs.257008.
3D structure databases
ProteinModelPortal	Q8IV08.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IV08. 2 interactions.
STRING	9606.ENSP00000348901.
PTM databases
PhosphoSite	Q8IV08.
Polymorphism databases
DMDM	74750647.
Proteomic databases
PaxDb	Q8IV08.
PRIDE	Q8IV08.
Protocols and materials databases
DNASU	23646.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356508; ENSP00000348901; ENSG00000105223. ENST00000409281; ENSP00000387022; ENSG00000105223. ENST00000409419; ENSP00000386293; ENSG00000105223. ENST00000409587; ENSP00000387050; ENSG00000105223. ENST00000409735; ENSP00000386938; ENSG00000105223.
GeneID	23646.
KEGG	hsa:23646.
UCSC	uc002onj.4. human.
Organism-specific databases
CTD	23646.
GeneCards	GC19P040854.
HGNC	HGNC:17158. PLD3.
HPA	CAB020812. HPA012800.
neXtProt	NX_Q8IV08.
PharmGKB	PA134887482.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG70733.
HOGENOM	HOG000293407.
HOVERGEN	HBG052880.
InParanoid	Q8IV08.
KO	K16860.
OMA	GKIFEAY.
PhylomeDB	Q8IV08.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q8IV08.
Bgee	Q8IV08.
CleanEx	HS_PLD3.
Genevestigator	Q8IV08.
Family and domain databases
InterPro	IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8IV08.
ChEMBL	CHEMBL2769.
ChiTaRS	PLD3. human.
GenomeRNAi	23646.
NextBio	46461.

Entry information

Entry name

PLD3_HUMAN

Accession

Primary (citable) accession number: Q8IV08
Secondary accession number(s): Q92853, Q9BW87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.