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Q8IUX7 (AEBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adipocyte enhancer-binding protein 1

Short name=AE-binding protein 1
Alternative name(s):
Aortic carboxypeptidase-like protein
Gene names
Name:AEBP1
Synonyms:ACLP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1158 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation By similarity. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness By similarity. Can act as a transcriptional repressor By similarity.

Subunit structure

Interacts with GNG5, NFKBIA, MAPK1, MAPK3 and PTEN By similarity. May interact with calmodulin By similarity. Binds to DNA in vitro By similarity.

Subcellular location

Isoform 1: Secreted By similarity.

Isoform 2: Cytoplasm Probable. Nucleus Probable.

Tissue specificity

Expressed in osteoblast and visceral fat. Ref.7

Post-translational modification

Phosphorylated by MAPK1 in vitro By similarity.

Sequence similarities

Belongs to the peptidase M14 family.

Contains 1 F5/8 type C domain.

Caution

Although related to peptidase M14 family, lacks the active sites residues and zinc-binding sites, suggesting that it has no carboxypeptidase activity.

Sequence caution

The sequence BAD92981.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   LigandCalmodulin-binding
DNA-binding
   Molecular functionRepressor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: InterPro

muscle organ development

Traceable author statement Ref.1. Source: ProtInc

skeletal system development

Traceable author statement Ref.7. Source: ProtInc

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space

Inferred from direct assay PubMed 20551380. Source: BHF-UCL

extracellular vesicular exosome

Inferred from direct assay PubMed 19199708. Source: UniProt

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

carboxypeptidase activity

Traceable author statement Ref.7. Source: ProtInc

metallocarboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Traceable author statement Ref.7. Source: ProtInc

transcription corepressor activity

Inferred from electronic annotation. Source: Ensembl

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IUX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IUX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-457: Missing.
     458-495: ITQGRDSSIH...VMYTNGYEEM → MRKWWAPCPG...STALRPASPQ
     543-544: AP → ARECGGLAGALSGGGVLGWASRHPAKDNPASLAA
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 11581133Adipocyte enhancer-binding protein 1
PRO_0000333189

Regions

Domain383 – 540158F5/8 type C
Region390 – 555166Required for DNA-binding and interaction with NFKBIA By similarity
Region421 – 624204Interaction with MAPK1 and MAPK3 By similarity
Region555 – 985431Interaction with PTEN By similarity
Region941 – 1158218Required for transcriptional repression By similarity
Region1006 – 1158153Interaction with MAPK1 and MAPK3 By similarity
Compositional bias47 – 326280Pro-rich
Compositional bias1079 – 113658Glu-rich

Amino acid modifications

Glycosylation5281N-linked (GlcNAc...) Ref.8
Glycosylation9221N-linked (GlcNAc...) Ref.8

Natural variations

Alternative sequence1 – 457457Missing in isoform 2.
VSP_033467
Alternative sequence458 – 49538ITQGR…GYEEM → MRKWWAPCPGSWLCSHCLGE GWALRGAGSTALRPASPQ in isoform 2.
VSP_033468
Alternative sequence543 – 5442AP → ARECGGLAGALSGGGVLGWA SRHPAKDNPASLAA in isoform 2.
VSP_033469
Natural variant2731P → T.
Corresponds to variant rs2537188 [ dbSNP | Ensembl ].
VAR_043118
Natural variant6481D → E.
Corresponds to variant rs11770649 [ dbSNP | Ensembl ].
VAR_043119
Natural variant10011P → L.
Corresponds to variant rs4724285 [ dbSNP | Ensembl ].
VAR_043120
Natural variant11331K → E. Ref.3
Corresponds to variant rs13928 [ dbSNP | Ensembl ].
VAR_043121
Natural variant11481V → I.
Corresponds to variant rs13898 [ dbSNP | Ensembl ].
VAR_043122

Experimental info

Sequence conflict1451K → E in AAC25585. Ref.1
Sequence conflict2181R → Q in AAC25585. Ref.1
Sequence conflict5691D → G in BAC87026. Ref.2
Sequence conflict7151R → G in BAC87026. Ref.2
Sequence conflict8841S → G in BAC87026. Ref.2
Sequence conflict10791E → G in BAD92981. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1D7F4A20451646AE

FASTA1,158130,929
        10         20         30         40         50         60 
MAAVRGAPLL SCLLALLALC PGGRPQTVLT DDEIEEFLEG FLSELEPEPR EDDVEAPPPP 

        70         80         90        100        110        120 
EPTPRVRKAQ AGGKPGKRPG TAAEVPPEKT KDKGKKGKKD KGPKVPKESL EGSPRPPKKG 

       130        140        150        160        170        180 
KEKPPKATKK PKEKPPKATK KPKEKPPKAT KKPKEKPPKA TKKPPSGKRP PILAPSETLE 

       190        200        210        220        230        240 
WPLPPPPSPG PEELPQEGGA PLSNNWQNPG EETHVEAREH QPEPEEETEQ PTLDYNDQIE 

       250        260        270        280        290        300 
REDYEDFEYI RRQKQPRPPP SRRRRPERVW PEPPEEKAPA PAPEERIEPP VKPLLPPLPP 

       310        320        330        340        350        360 
DYGDGYVIPN YDDMDYYFGP PPPQKPDAER QTDEEKEELK KPKKEDSSPK EETDKWAVEK 

       370        380        390        400        410        420 
GKDHKEPRKG EELEEEWTPT EKVKCPPIGM ESHRIEDNQI RASSMLRHGL GAQRGRLNMQ 

       430        440        450        460        470        480 
TGATEDDYYD GAWCAEDDAR TQWIEVDTRR TTRFTGVITQ GRDSSIHDDF VTTFFVGFSN 

       490        500        510        520        530        540 
DSQTWVMYTN GYEEMTFHGN VDKDTPVLSE LPEPVVARFI RIYPLTWNGS LCMRLEVLGC 

       550        560        570        580        590        600 
SVAPVYSYYA QNEVVATDDL DFRHHSYKDM RQLMKVVNEE CPTITRTYSL GKSSRGLKIY 

       610        620        630        640        650        660 
AMEISDNPGE HELGEPEFRY TAGIHGNEVL GRELLLLLMQ YLCREYRDGN PRVRSLVQDT 

       670        680        690        700        710        720 
RIHLVPSLNP DGYEVAAQMG SEFGNWALGL WTEEGFDIFE DFPDLNSVLW GAEERKWVPY 

       730        740        750        760        770        780 
RVPNNNLPIP ERYLSPDATV STEVRAIIAW MEKNPFVLGA NLNGGERLVS YPYDMARTPT 

       790        800        810        820        830        840 
QEQLLAAAMA AARGEDEDEV SEAQETPDHA IFRWLAISFA SAHLTLTEPY RGGCQAQDYT 

       850        860        870        880        890        900 
GGMGIVNGAK WNPRTGTIND FSYLHTNCLE LSFYLGCDKF PHESELPREW ENNKEALLTF 

       910        920        930        940        950        960 
MEQVHRGIKG VVTDEQGIPI ANATISVSGI NHGVKTASGG DYWRILNPGE YRVTAHAEGY 

       970        980        990       1000       1010       1020 
TPSAKTCNVD YDIGATQCNF ILARSNWKRI REIMAMNGNR PIPHIDPSRP MTPQQRRLQQ 

      1030       1040       1050       1060       1070       1080 
RRLQHRLRLR AQMRLRRLNA TTTLGPHTVP PTLPPAPATT LSTTIEPWGL IPPTTAGWEE 

      1090       1100       1110       1120       1130       1140 
SETETYTEVV TEFGTEVEPE FGTKVEPEFE TQLEPEFETQ LEPEFEEEEE EEKEEEIATG 

      1150 
QAFPFTTVET YTVNFGDF 

« Hide

Isoform 2 [UniParc].

Checksum: A749B43E37811E45
Show »

FASTA73382,183

References

« Hide 'large scale' references
[1]"Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiation."
Layne M.D., Endege W.O., Jain M.K., Yet S.-F., Hsieh C.-M., Chin M.T., Perrella M.A., Blanar M.A., Haber E., Lee M.-E.
J. Biol. Chem. 273:15654-15660(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Aortic smooth muscle.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLU-1133.
Tissue: Aortic endothelium.
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"A cDNA cloning of human AEBP1 from primary cultured osteoblasts and its expression in a differentiating osteoblastic cell line."
Ohno I., Hashimoto J., Shimizu K., Takaoka K., Ochi T., Matsubara K., Okubo K.
Biochem. Biophys. Res. Commun. 228:411-414(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-1158 (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Cancellous bone.
[8]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-528 AND ASN-922.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053944 mRNA. Translation: AAC25585.1.
AK127541 mRNA. Translation: BAC87026.1.
AB209744 mRNA. Translation: BAD92981.1. Different initiation.
CH236960 Genomic DNA. Translation: EAL23768.1.
CH471128 Genomic DNA. Translation: EAW61119.1.
BC038588 mRNA. Translation: AAH38588.1.
D86479 mRNA. Translation: BAA13094.1.
CCDSCCDS5476.1. [Q8IUX7-1]
PIRJC5256.
RefSeqNP_001120.3. NM_001129.4. [Q8IUX7-1]
UniGeneHs.439463.

3D structure databases

ProteinModelPortalQ8IUX7.
SMRQ8IUX7. Positions 387-542, 560-1018.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106674. 1 interaction.
IntActQ8IUX7. 2 interactions.
MINTMINT-7034542.
STRING9606.ENSP00000223357.

Protein family/group databases

MEROPSM14.951.

PTM databases

PhosphoSiteQ8IUX7.

Polymorphism databases

DMDM74728002.

Proteomic databases

MaxQBQ8IUX7.
PaxDbQ8IUX7.
PRIDEQ8IUX7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000223357; ENSP00000223357; ENSG00000106624. [Q8IUX7-1]
ENST00000450684; ENSP00000398878; ENSG00000106624. [Q8IUX7-2]
GeneID165.
KEGGhsa:165.
UCSCuc003tkb.4. human. [Q8IUX7-1]
uc003tkc.5. human. [Q8IUX7-2]

Organism-specific databases

CTD165.
GeneCardsGC07P044143.
HGNCHGNC:303. AEBP1.
HPACAB009966.
MIM602981. gene.
neXtProtNX_Q8IUX7.
PharmGKBPA24604.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG292169.
HOGENOMHOG000232185.
HOVERGENHBG003410.
InParanoidQ8IUX7.
OMALDYNDQI.
OrthoDBEOG7B8S32.
PhylomeDBQ8IUX7.
TreeFamTF315592.

Gene expression databases

ArrayExpressQ8IUX7.
BgeeQ8IUX7.
CleanExHS_AEBP1.
GenevestigatorQ8IUX7.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSPR00765. CRBOXYPTASEA.
SMARTSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAEBP1. human.
GenomeRNAi165.
NextBio665.
PROQ8IUX7.
SOURCESearch...

Entry information

Entry nameAEBP1_HUMAN
AccessionPrimary (citable) accession number: Q8IUX7
Secondary accession number(s): Q14113 expand/collapse secondary AC list , Q59ER7, Q6ZSC7, Q7KZ79
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM