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Reviewed, UniProtKB/Swiss-Prot Q8IUX7 (AEBP1_HUMAN)

Last modified January 19, 2010. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Adipocyte enhancer-binding protein 1
      Short name=AE-binding protein 1
Alternative name(s):
    Aortic carboxypeptidase-like protein
Gene names
Name: AEBP1
Synonyms: ACLP
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1158 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation By similarity. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness By similarity. Can act as a transcriptional repressor By similarity.

Subunit structure

Interacts with GNG5, NFKBIA, MAPK1, MAPK3 and PTEN By similarity. May interact with calmodulin By similarity. Binds to DNA in vitro By similarity.

Subcellular location

Isoform 1: Secreted By similarity.

Isoform 2: Cytoplasm Probable. Nucleus Probable.

Tissue specificity

Expressed in osteoblast and visceral fat. Ref.7

Post-translational modification

Phosphorylated by MAPK1 in vitro By similarity.

Sequence similarities

Belongs to the peptidase M14 family.

Contains 1 F5/8 type C domain.

Caution

This protein has lost active site residues and zinc-binding sites and so is unlikely to be catalytically active.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IUX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IUX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-457: Missing.
     458-495: ITQGRDSSIH...VMYTNGYEEM → MRKWWAPCPG...STALRPASPQ
     543-544: AP → ARECGGLAGALSGGGVLGWASRHPAKDNPASLAA
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 11581133Adipocyte enhancer-binding protein 1
PRO_0000333189

Regions

Domain383 – 540158F5/8 type C
Region390 – 555166Required for DNA-binding and interaction with NFKBIA By similarity
Region421 – 624204Interaction with MAPK1 and MAPK3 By similarity
Region555 – 985431Interaction with PTEN By similarity
Region941 – 1158218Required for transcriptional repression By similarity
Region1006 – 1158153Interaction with MAPK1 and MAPK3 By similarity
Compositional bias47 – 326280Pro-rich
Compositional bias1079 – 113658Glu-rich

Amino acid modifications

Glycosylation5281N-linked (GlcNAc...) Ref.8
Glycosylation9221N-linked (GlcNAc...) Ref.8

Natural variations

Alternative sequence1 – 457457Missing in isoform 2.
VSP_033467
Alternative sequence458 – 49538ITQGR…GYEEM → MRKWWAPCPGSWLCSHCLGE GWALRGAGSTALRPASPQ in isoform 2.
VSP_033468
Alternative sequence543 – 5442AP → ARECGGLAGALSGGGVLGWA SRHPAKDNPASLAA in isoform 2.
VSP_033469
Natural variant2731P → T: dbSNP rs2537188.
VAR_043118
Natural variant6481D → E: dbSNP rs11770649.
VAR_043119
Natural variant10011P → L: dbSNP rs4724285.
VAR_043120
Natural variant11331K → E: dbSNP rs13928. Ref.3
VAR_043121
Natural variant11481V → I: dbSNP rs13898.
VAR_043122

Experimental info

Sequence conflict1451K → E in AAC25585. Ref.1
Sequence conflict2181R → Q in AAC25585. Ref.1
Sequence conflict5691D → G in BAC87026. Ref.2
Sequence conflict7151R → G in BAC87026. Ref.2
Sequence conflict8841S → G in BAC87026. Ref.2
Sequence conflict10791E → G in BAD92981. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1D7F4A20451646AE

FASTA1,158130,929
        10         20         30         40         50         60 
MAAVRGAPLL SCLLALLALC PGGRPQTVLT DDEIEEFLEG FLSELEPEPR EDDVEAPPPP 

        70         80         90        100        110        120 
EPTPRVRKAQ AGGKPGKRPG TAAEVPPEKT KDKGKKGKKD KGPKVPKESL EGSPRPPKKG 

       130        140        150        160        170        180 
KEKPPKATKK PKEKPPKATK KPKEKPPKAT KKPKEKPPKA TKKPPSGKRP PILAPSETLE 

       190        200        210        220        230        240 
WPLPPPPSPG PEELPQEGGA PLSNNWQNPG EETHVEAREH QPEPEEETEQ PTLDYNDQIE 

       250        260        270        280        290        300 
REDYEDFEYI RRQKQPRPPP SRRRRPERVW PEPPEEKAPA PAPEERIEPP VKPLLPPLPP 

       310        320        330        340        350        360 
DYGDGYVIPN YDDMDYYFGP PPPQKPDAER QTDEEKEELK KPKKEDSSPK EETDKWAVEK 

       370        380        390        400        410        420 
GKDHKEPRKG EELEEEWTPT EKVKCPPIGM ESHRIEDNQI RASSMLRHGL GAQRGRLNMQ 

       430        440        450        460        470        480 
TGATEDDYYD GAWCAEDDAR TQWIEVDTRR TTRFTGVITQ GRDSSIHDDF VTTFFVGFSN 

       490        500        510        520        530        540 
DSQTWVMYTN GYEEMTFHGN VDKDTPVLSE LPEPVVARFI RIYPLTWNGS LCMRLEVLGC 

       550        560        570        580        590        600 
SVAPVYSYYA QNEVVATDDL DFRHHSYKDM RQLMKVVNEE CPTITRTYSL GKSSRGLKIY 

       610        620        630        640        650        660 
AMEISDNPGE HELGEPEFRY TAGIHGNEVL GRELLLLLMQ YLCREYRDGN PRVRSLVQDT 

       670        680        690        700        710        720 
RIHLVPSLNP DGYEVAAQMG SEFGNWALGL WTEEGFDIFE DFPDLNSVLW GAEERKWVPY 

       730        740        750        760        770        780 
RVPNNNLPIP ERYLSPDATV STEVRAIIAW MEKNPFVLGA NLNGGERLVS YPYDMARTPT 

       790        800        810        820        830        840 
QEQLLAAAMA AARGEDEDEV SEAQETPDHA IFRWLAISFA SAHLTLTEPY RGGCQAQDYT 

       850        860        870        880        890        900 
GGMGIVNGAK WNPRTGTIND FSYLHTNCLE LSFYLGCDKF PHESELPREW ENNKEALLTF 

       910        920        930        940        950        960 
MEQVHRGIKG VVTDEQGIPI ANATISVSGI NHGVKTASGG DYWRILNPGE YRVTAHAEGY 

       970        980        990       1000       1010       1020 
TPSAKTCNVD YDIGATQCNF ILARSNWKRI REIMAMNGNR PIPHIDPSRP MTPQQRRLQQ 

      1030       1040       1050       1060       1070       1080 
RRLQHRLRLR AQMRLRRLNA TTTLGPHTVP PTLPPAPATT LSTTIEPWGL IPPTTAGWEE 

      1090       1100       1110       1120       1130       1140 
SETETYTEVV TEFGTEVEPE FGTKVEPEFE TQLEPEFETQ LEPEFEEEEE EEKEEEIATG 

      1150 
QAFPFTTVET YTVNFGDF 

« Hide

Isoform 2.

Checksum: A749B43E37811E45
Show »

FASTA73382,183

References

« Hide 'large scale' references
[1]"Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiation."
Layne M.D., Endege W.O., Jain M.K., Yet S.-F., Hsieh C.-M., Chin M.T., Perrella M.A., Blanar M.A., Haber E., Lee M.-E.
J. Biol. Chem. 273:15654-15660(1998) [PubMed: 9624159] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Aortic smooth muscle.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLU-1133.
Tissue: Aortic endothelium.
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed: 12690205] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"A cDNA cloning of human AEBP1 from primary cultured osteoblasts and its expression in a differentiating osteoblastic cell line."
Ohno I., Hashimoto J., Shimizu K., Takaoka K., Ochi T., Matsubara K., Okubo K.
Biochem. Biophys. Res. Commun. 228:411-414(1996) [PubMed: 8920928] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-1158 (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Cancellous bone.
[8]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-528 AND ASN-922, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053944 mRNA. Translation: AAC25585.1.
AK127541 mRNA. Translation: BAC87026.1.
AB209744 mRNA. Translation: BAD92981.1. Different initiation.
CH236960 Genomic DNA. Translation: EAL23768.1.
CH471128 Genomic DNA. Translation: EAW61119.1.
BC038588 mRNA. Translation: AAH38588.1.
D86479 mRNA. Translation: BAA13094.1.
IPIIPI00745313.
IPI00894097.
PIRJC5256.
RefSeqNP_001120.3.
UniGeneHs.439463

3D structure databases

HSSPHSSP built from PDB template 1H8L based on UniProtKB P83852.
SMRQ8IUX7. Positions 383-541, 559-985.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8IUX7.

Protein family/group databases

MEROPSM14.951.

PTM databases

PhosphoSiteQ8IUX7.

Proteomic databases

PRIDEQ8IUX7.

Genome annotation databases

EnsemblENST00000223357; ENSP00000223357; ENSG00000106624; Homo sapiens. [Genome view]
GeneID165.
KEGGhsa:165.
UCSCuc003tkb.1. human.
uc003tkc.2. human.

Organism-specific databases

CTD165.
GeneCardsGC07P044110.
HGNCHGNC:303. AEBP1.
HPACAB009966.
MIM602981. gene.
PharmGKBPA24604.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG19048.
HOGENOMHBG599263.
HOVERGENQ8IUX7.
InParanoidQ8IUX7.
OMALEEEWAP.
OrthoDBEOG90KBJP.
PhylomeDBQ8IUX7.

Gene expression databases

ArrayExpressQ8IUX7.
BgeeQ8IUX7.
CleanExHS_AEBP1.
GenevestigatorQ8IUX7.

Family and domain databases

InterProIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. Coagulation_factor_5/8-type_C.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
Gene3DG3DSA:2.60.40.1120. CarboxyPept_regulatory. 1 hit.
PfamPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSPR00765. CRBOXYPTASEA.
SMARTSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
PROSITEPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio665.
SOURCESearch...

Entry information

Entry nameAEBP1_HUMAN
AccessionPrimary (citable) accession number: Q8IUX7
Secondary accession number(s): Q14113 expand/collapse secondary AC list , Q59ER7, Q6ZSC7, Q7KZ79
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2003
Last modified: January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents