Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adipocyte enhancer-binding protein 1

Gene

AEBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.By similarity

GO - Molecular functioni

GO - Biological processi

  • muscle organ development Source: ProtInc
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • peptide metabolic process Source: GO_Central
  • protein processing Source: GO_Central
  • skeletal system development Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Calmodulin-binding, DNA-binding

Protein family/group databases

MEROPSiM14.951.

Names & Taxonomyi

Protein namesi
Recommended name:
Adipocyte enhancer-binding protein 1
Short name:
AE-binding protein 1
Alternative name(s):
Aortic carboxypeptidase-like protein
Gene namesi
Name:AEBP1
Synonyms:ACLP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:303. AEBP1.

Subcellular locationi

Isoform 1 :
  • Secreted By similarity
Isoform 2 :
  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: BHF-UCL
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi165.
OpenTargetsiENSG00000106624.
PharmGKBiPA24604.

Polymorphism and mutation databases

DMDMi74728002.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000033318926 – 1158Adipocyte enhancer-binding protein 1Add BLAST1133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi528N-linked (GlcNAc...)1 Publication1
Glycosylationi922N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

Phosphorylated by MAPK1 in vitro.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8IUX7.
MaxQBiQ8IUX7.
PaxDbiQ8IUX7.
PeptideAtlasiQ8IUX7.
PRIDEiQ8IUX7.

PTM databases

iPTMnetiQ8IUX7.
PhosphoSitePlusiQ8IUX7.

Expressioni

Tissue specificityi

Expressed in osteoblast and visceral fat.1 Publication

Gene expression databases

BgeeiENSG00000106624.
CleanExiHS_AEBP1.
ExpressionAtlasiQ8IUX7. baseline and differential.
GenevisibleiQ8IUX7. HS.

Organism-specific databases

HPAiCAB009966.
HPA063595.
HPA064970.

Interactioni

Subunit structurei

Interacts with GNG5, NFKBIA, MAPK1, MAPK3 and PTEN. May interact with calmodulin. Binds to DNA in vitro.By similarity

Protein-protein interaction databases

BioGridi106674. 1 interactor.
IntActiQ8IUX7. 2 interactors.
MINTiMINT-7034542.
STRINGi9606.ENSP00000223357.

Structurei

3D structure databases

ProteinModelPortaliQ8IUX7.
SMRiQ8IUX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini383 – 540F5/8 type CPROSITE-ProRule annotationAdd BLAST158

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 555Required for DNA-binding and interaction with NFKBIABy similarityAdd BLAST166
Regioni421 – 624Interaction with MAPK1 and MAPK3By similarityAdd BLAST204
Regioni555 – 985Interaction with PTENBy similarityAdd BLAST431
Regioni941 – 1158Required for transcriptional repressionBy similarityAdd BLAST218
Regioni1006 – 1158Interaction with MAPK1 and MAPK3By similarityAdd BLAST153

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi47 – 326Pro-richAdd BLAST280
Compositional biasi1079 – 1136Glu-richAdd BLAST58

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ8IUX7.
OMAiTLDYNDQ.
OrthoDBiEOG091G06A9.
PhylomeDBiQ8IUX7.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IUX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVRGAPLL SCLLALLALC PGGRPQTVLT DDEIEEFLEG FLSELEPEPR
60 70 80 90 100
EDDVEAPPPP EPTPRVRKAQ AGGKPGKRPG TAAEVPPEKT KDKGKKGKKD
110 120 130 140 150
KGPKVPKESL EGSPRPPKKG KEKPPKATKK PKEKPPKATK KPKEKPPKAT
160 170 180 190 200
KKPKEKPPKA TKKPPSGKRP PILAPSETLE WPLPPPPSPG PEELPQEGGA
210 220 230 240 250
PLSNNWQNPG EETHVEAREH QPEPEEETEQ PTLDYNDQIE REDYEDFEYI
260 270 280 290 300
RRQKQPRPPP SRRRRPERVW PEPPEEKAPA PAPEERIEPP VKPLLPPLPP
310 320 330 340 350
DYGDGYVIPN YDDMDYYFGP PPPQKPDAER QTDEEKEELK KPKKEDSSPK
360 370 380 390 400
EETDKWAVEK GKDHKEPRKG EELEEEWTPT EKVKCPPIGM ESHRIEDNQI
410 420 430 440 450
RASSMLRHGL GAQRGRLNMQ TGATEDDYYD GAWCAEDDAR TQWIEVDTRR
460 470 480 490 500
TTRFTGVITQ GRDSSIHDDF VTTFFVGFSN DSQTWVMYTN GYEEMTFHGN
510 520 530 540 550
VDKDTPVLSE LPEPVVARFI RIYPLTWNGS LCMRLEVLGC SVAPVYSYYA
560 570 580 590 600
QNEVVATDDL DFRHHSYKDM RQLMKVVNEE CPTITRTYSL GKSSRGLKIY
610 620 630 640 650
AMEISDNPGE HELGEPEFRY TAGIHGNEVL GRELLLLLMQ YLCREYRDGN
660 670 680 690 700
PRVRSLVQDT RIHLVPSLNP DGYEVAAQMG SEFGNWALGL WTEEGFDIFE
710 720 730 740 750
DFPDLNSVLW GAEERKWVPY RVPNNNLPIP ERYLSPDATV STEVRAIIAW
760 770 780 790 800
MEKNPFVLGA NLNGGERLVS YPYDMARTPT QEQLLAAAMA AARGEDEDEV
810 820 830 840 850
SEAQETPDHA IFRWLAISFA SAHLTLTEPY RGGCQAQDYT GGMGIVNGAK
860 870 880 890 900
WNPRTGTIND FSYLHTNCLE LSFYLGCDKF PHESELPREW ENNKEALLTF
910 920 930 940 950
MEQVHRGIKG VVTDEQGIPI ANATISVSGI NHGVKTASGG DYWRILNPGE
960 970 980 990 1000
YRVTAHAEGY TPSAKTCNVD YDIGATQCNF ILARSNWKRI REIMAMNGNR
1010 1020 1030 1040 1050
PIPHIDPSRP MTPQQRRLQQ RRLQHRLRLR AQMRLRRLNA TTTLGPHTVP
1060 1070 1080 1090 1100
PTLPPAPATT LSTTIEPWGL IPPTTAGWEE SETETYTEVV TEFGTEVEPE
1110 1120 1130 1140 1150
FGTKVEPEFE TQLEPEFETQ LEPEFEEEEE EEKEEEIATG QAFPFTTVET

YTVNFGDF
Length:1,158
Mass (Da):130,929
Last modified:March 1, 2003 - v1
Checksum:i1D7F4A20451646AE
GO
Isoform 2 (identifier: Q8IUX7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-457: Missing.
     458-495: ITQGRDSSIH...VMYTNGYEEM → MRKWWAPCPG...STALRPASPQ
     543-544: AP → ARECGGLAGALSGGGVLGWASRHPAKDNPASLAA

Note: No experimental confirmation available.
Show »
Length:733
Mass (Da):82,183
Checksum:iA749B43E37811E45
GO

Sequence cautioni

The sequence BAD92981 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145K → E in AAC25585 (PubMed:9624159).Curated1
Sequence conflicti218R → Q in AAC25585 (PubMed:9624159).Curated1
Sequence conflicti569D → G in BAC87026 (PubMed:14702039).Curated1
Sequence conflicti715R → G in BAC87026 (PubMed:14702039).Curated1
Sequence conflicti884S → G in BAC87026 (PubMed:14702039).Curated1
Sequence conflicti1079E → G in BAD92981 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_043118273P → T.Corresponds to variant rs2537188dbSNPEnsembl.1
Natural variantiVAR_043119648D → E.Corresponds to variant rs11770649dbSNPEnsembl.1
Natural variantiVAR_0431201001P → L.Corresponds to variant rs4724285dbSNPEnsembl.1
Natural variantiVAR_0431211133K → E.1 PublicationCorresponds to variant rs13928dbSNPEnsembl.1
Natural variantiVAR_0431221148V → I.Corresponds to variant rs13898dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0334671 – 457Missing in isoform 2. 1 PublicationAdd BLAST457
Alternative sequenceiVSP_033468458 – 495ITQGR…GYEEM → MRKWWAPCPGSWLCSHCLGE GWALRGAGSTALRPASPQ in isoform 2. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_033469543 – 544AP → ARECGGLAGALSGGGVLGWA SRHPAKDNPASLAA in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053944 mRNA. Translation: AAC25585.1.
AK127541 mRNA. Translation: BAC87026.1.
AB209744 mRNA. Translation: BAD92981.1. Different initiation.
CH236960 Genomic DNA. Translation: EAL23768.1.
CH471128 Genomic DNA. Translation: EAW61119.1.
BC038588 mRNA. Translation: AAH38588.1.
D86479 mRNA. Translation: BAA13094.1.
CCDSiCCDS5476.1. [Q8IUX7-1]
PIRiJC5256.
RefSeqiNP_001120.3. NM_001129.4. [Q8IUX7-1]
UniGeneiHs.439463.

Genome annotation databases

EnsembliENST00000223357; ENSP00000223357; ENSG00000106624. [Q8IUX7-1]
ENST00000450684; ENSP00000398878; ENSG00000106624. [Q8IUX7-2]
GeneIDi165.
KEGGihsa:165.
UCSCiuc003tkb.5. human. [Q8IUX7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053944 mRNA. Translation: AAC25585.1.
AK127541 mRNA. Translation: BAC87026.1.
AB209744 mRNA. Translation: BAD92981.1. Different initiation.
CH236960 Genomic DNA. Translation: EAL23768.1.
CH471128 Genomic DNA. Translation: EAW61119.1.
BC038588 mRNA. Translation: AAH38588.1.
D86479 mRNA. Translation: BAA13094.1.
CCDSiCCDS5476.1. [Q8IUX7-1]
PIRiJC5256.
RefSeqiNP_001120.3. NM_001129.4. [Q8IUX7-1]
UniGeneiHs.439463.

3D structure databases

ProteinModelPortaliQ8IUX7.
SMRiQ8IUX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106674. 1 interactor.
IntActiQ8IUX7. 2 interactors.
MINTiMINT-7034542.
STRINGi9606.ENSP00000223357.

Protein family/group databases

MEROPSiM14.951.

PTM databases

iPTMnetiQ8IUX7.
PhosphoSitePlusiQ8IUX7.

Polymorphism and mutation databases

DMDMi74728002.

Proteomic databases

EPDiQ8IUX7.
MaxQBiQ8IUX7.
PaxDbiQ8IUX7.
PeptideAtlasiQ8IUX7.
PRIDEiQ8IUX7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223357; ENSP00000223357; ENSG00000106624. [Q8IUX7-1]
ENST00000450684; ENSP00000398878; ENSG00000106624. [Q8IUX7-2]
GeneIDi165.
KEGGihsa:165.
UCSCiuc003tkb.5. human. [Q8IUX7-1]

Organism-specific databases

CTDi165.
DisGeNETi165.
GeneCardsiAEBP1.
HGNCiHGNC:303. AEBP1.
HPAiCAB009966.
HPA063595.
HPA064970.
MIMi602981. gene.
neXtProtiNX_Q8IUX7.
OpenTargetsiENSG00000106624.
PharmGKBiPA24604.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ8IUX7.
OMAiTLDYNDQ.
OrthoDBiEOG091G06A9.
PhylomeDBiQ8IUX7.
TreeFamiTF315592.

Miscellaneous databases

ChiTaRSiAEBP1. human.
GenomeRNAii165.
PROiQ8IUX7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106624.
CleanExiHS_AEBP1.
ExpressionAtlasiQ8IUX7. baseline and differential.
GenevisibleiQ8IUX7. HS.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAEBP1_HUMAN
AccessioniPrimary (citable) accession number: Q8IUX7
Secondary accession number(s): Q14113
, Q59ER7, Q6ZSC7, Q7KZ79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although related to peptidase M14 family, lacks the active sites residues and zinc-binding sites, suggesting that it has no carboxypeptidase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.