ID   EPHX4_HUMAN             Reviewed;         362 AA.
AC   Q8IUS5; Q8NCC6;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 2.
DT   27-MAR-2024, entry version 152.
DE   RecName: Full=Epoxide hydrolase 4;
DE            EC=3.3.-.-;
DE   AltName: Full=Abhydrolase domain-containing protein 7;
DE   AltName: Full=Epoxide hydrolase-related protein;
GN   Name=EPHX4; Synonyms=ABHD7, EH4, EPHXRP;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Teratocarcinoma;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-321.
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- INTERACTION:
CC       Q8IUS5; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-17962164, EBI-8638294;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Epoxide hydrolase
CC       family. {ECO:0000305}.
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DR   EMBL; AK074822; BAC11230.1; -; mRNA.
DR   EMBL; BC041475; AAH41475.1; -; mRNA.
DR   CCDS; CCDS736.1; -.
DR   RefSeq; NP_775838.3; NM_173567.4.
DR   AlphaFoldDB; Q8IUS5; -.
DR   SMR; Q8IUS5; -.
DR   BioGRID; 128958; 41.
DR   IntAct; Q8IUS5; 1.
DR   STRING; 9606.ENSP00000359410; -.
DR   ESTHER; human-EPHX4; Epoxide_hydrolase.
DR   MEROPS; S33.974; -.
DR   iPTMnet; Q8IUS5; -.
DR   PhosphoSitePlus; Q8IUS5; -.
DR   BioMuta; EPHX4; -.
DR   DMDM; 134035378; -.
DR   EPD; Q8IUS5; -.
DR   jPOST; Q8IUS5; -.
DR   MassIVE; Q8IUS5; -.
DR   MaxQB; Q8IUS5; -.
DR   PaxDb; 9606-ENSP00000359410; -.
DR   PeptideAtlas; Q8IUS5; -.
DR   ProteomicsDB; 70611; -.
DR   Pumba; Q8IUS5; -.
DR   Antibodypedia; 33635; 100 antibodies from 17 providers.
DR   DNASU; 253152; -.
DR   Ensembl; ENST00000370383.5; ENSP00000359410.4; ENSG00000172031.7.
DR   GeneID; 253152; -.
DR   KEGG; hsa:253152; -.
DR   MANE-Select; ENST00000370383.5; ENSP00000359410.4; NM_173567.5; NP_775838.3.
DR   UCSC; uc001don.3; human.
DR   AGR; HGNC:23758; -.
DR   CTD; 253152; -.
DR   GeneCards; EPHX4; -.
DR   HGNC; HGNC:23758; EPHX4.
DR   HPA; ENSG00000172031; Group enriched (brain, choroid plexus).
DR   MIM; 617401; gene.
DR   neXtProt; NX_Q8IUS5; -.
DR   OpenTargets; ENSG00000172031; -.
DR   PharmGKB; PA164719207; -.
DR   VEuPathDB; HostDB:ENSG00000172031; -.
DR   eggNOG; KOG4178; Eukaryota.
DR   GeneTree; ENSGT00940000156233; -.
DR   HOGENOM; CLU_020336_7_3_1; -.
DR   InParanoid; Q8IUS5; -.
DR   OMA; YRWMVRS; -.
DR   OrthoDB; 2443908at2759; -.
DR   PhylomeDB; Q8IUS5; -.
DR   TreeFam; TF314403; -.
DR   PathwayCommons; Q8IUS5; -.
DR   SignaLink; Q8IUS5; -.
DR   BioGRID-ORCS; 253152; 8 hits in 1153 CRISPR screens.
DR   ChiTaRS; EPHX4; human.
DR   GenomeRNAi; 253152; -.
DR   Pharos; Q8IUS5; Tbio.
DR   PRO; PR:Q8IUS5; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q8IUS5; Protein.
DR   Bgee; ENSG00000172031; Expressed in primordial germ cell in gonad and 108 other cell types or tissues.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004301; F:epoxide hydrolase activity; IEA:UniProt.
DR   GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   PANTHER; PTHR43329; EPOXIDE HYDROLASE; 1.
DR   PANTHER; PTHR43329:SF2; EPOXIDE HYDROLASE 4; 1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   Genevisible; Q8IUS5; HS.
PE   1: Evidence at protein level;
KW   Hydrolase; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..362
FT                   /note="Epoxide hydrolase 4"
FT                   /id="PRO_0000281378"
FT   TRANSMEM        17..37
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          94..211
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        169
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        281
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        336
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   VARIANT         321
FT                   /note="Y -> F (in dbSNP:rs17854127)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_031235"
FT   CONFLICT        285
FT                   /note="F -> I (in Ref. 1; BAC11230)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  42324 MW;  B0B359E0AF0EED7C CRC64;
     MARLRDCLPR LMLTLRSLLF WSLVYCYCGL CASIHLLKLL WSLGKGPAQT FRRPAREHPP
     ACLSDPSLGT HCYVRIKDSG LRFHYVAAGE RGKPLMLLLH GFPEFWYSWR YQLREFKSEY
     RVVALDLRGY GETDAPIHRQ NYKLDCLITD IKDILDSLGY SKCVLIGHDW GGMIAWLIAI
     CYPEMVMKLI VINFPHPNVF TEYILRHPAQ LLKSSYYYFF QIPWFPEFMF SINDFKVLKH
     LFTSHSTGIG RKGCQLTTED LEAYIYVFSQ PGALSGPINH YRNIFSCLPL KHHMVTTPTL
     LLWGENDAFM EVEMAEVTKI YVKNYFRLTI LSEASHWLQQ DQPDIVNKLI WTFLKEETRK
     KD
//