Reviewed, UniProtKB/Swiss-Prot Q8IUN9 (CLC10_HUMAN)

Names and origin

Protein names

Recommended name:
    C-type lectin domain family 10 member A
Alternative name(s):
    C-type lectin superfamily member 14
    Macrophage lectin 2
    CD_antigen=CD301

Gene names

Name:	CLEC10A
Synonyms:	CLECSF13, CLECSF14, HML

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. Ref.1
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Biological process	Endocytosis Immune response
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Signal-anchor Transmembrane
Ligand	Lectin
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	sugar binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IUN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IUN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-144: Missing.
     173-173: N → NGEE

Isoform 3 (identifier: Q8IUN9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     118-144: Missing.
     226-283: NFVQKYLGSA...HGLGGGEDCA → VRASGTQFLR...IQRNISKLLS
     284-316: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	C-type lectin domain family 10 member A		PRO_0000046639
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Signal-anchor for type II membrane protein Potential
	Topological domain	61 – 316	256	Extracellular Potential
	Domain	188 – 305	118	C-type lectin
	Coiled coil	85 – 176	92	Potential
	Motif	5 – 8	4	Endocytosis signal Potential
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Disulfide bond	181 ↔ 192		By similarity
	Disulfide bond	209 ↔ 304		By similarity
	Disulfide bond	282 ↔ 296		By similarity
Natural variations
	Alternative sequence	118 – 144	27	Missing in isoform 2 and isoform 3.		VSP_012848
	Alternative sequence	173	1	N → NGEE in isoform 2.		VSP_012849
	Alternative sequence	226 – 283	58	NFVQK…GEDCA → VRASGTQFLRHVPFREMVLK LGRTLESSGSFQNDCHLCHT LRDLIGLSIQRNISKLLS in isoform 3.		VSP_012850
	Alternative sequence	284 – 316	33	Missing in isoform 3.		VSP_012851
	Natural variant	35	1	C → R: dbSNP rs90951. Ref.1		VAR_021262
	Natural variant	73	1	R → K: dbSNP rs16956478.		VAR_050113
	Natural variant	100	1	T → M: dbSNP rs35318160.		VAR_050114
	Natural variant	203	1	A → G: dbSNP rs35101468.		VAR_050115
Experimental info
	Sequence conflict	112	1	K → R in AAH27858. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D3B7193E2E1F58AF	FASTA	316	35,446
	10 20 30 40 50 60 MTRTYENFQY LENKVKVQGF KNGPLPLQSL LQRLCSGPCH LLLSLGLGLL LLVIICVVGF 70 80 90 100 110 120 QNSKFQRDLV TLRTDFSNFT SNTVAEIQAL TSQGSSLEET IASLKAEVEG FKQERQAGVS 130 140 150 160 170 180 ELQEHTTQKA HLGHCPHCPS VCVPVHSEML LRVQQLVQDL KKLTCQVATL NNNASTEGTC 190 200 210 220 230 240 CPVNWVEHQD SCYWFSHSGM SWAEAEKYCQ LKNAHLVVIN SREEQNFVQK YLGSAYTWMG 250 260 270 280 290 300 LSDPEGAWKW VDGTDYATGF QNWKPGQPDD WQGHGLGGGE DCAHFHPDGR WNDDVCQRPY 310 HWVCEAGLGQ TSQESH
	Isoform 2. Checksum: 5472EFF9EBC7AC19	FASTA	292	32,884
	10 20 30 40 50 60 MTRTYENFQY LENKVKVQGF KNGPLPLQSL LQRLCSGPCH LLLSLGLGLL LLVIICVVGF 70 80 90 100 110 120 QNSKFQRDLV TLRTDFSNFT SNTVAEIQAL TSQGSSLEET IASLKAEVEG FKQERQAVHS 130 140 150 160 170 180 EMLLRVQQLV QDLKKLTCQV ATLNNNGEEA STEGTCCPVN WVEHQDSCYW FSHSGMSWAE 190 200 210 220 230 240 AEKYCQLKNA HLVVINSREE QNFVQKYLGS AYTWMGLSDP EGAWKWVDGT DYATGFQNWK 250 260 270 280 290 PGQPDDWQGH GLGGGEDCAH FHPDGRWNDD VCQRPYHWVC EAGLGQTSQE SH
	Isoform 3. Checksum: BEB86B3B5CC8DCD0	FASTA	256	28,877
	10 20 30 40 50 60 MTRTYENFQY LENKVKVQGF KNGPLPLQSL LQRLCSGPCH LLLSLGLGLL LLVIICVVGF 70 80 90 100 110 120 QNSKFQRDLV TLRTDFSNFT SNTVAEIQAL TSQGSSLEET IASLKAEVEG FKQERQAVHS 130 140 150 160 170 180 EMLLRVQQLV QDLKKLTCQV ATLNNNASTE GTCCPVNWVE HQDSCYWFSH SGMSWAEAEK 190 200 210 220 230 240 YCQLKNAHLV VINSREEQVR ASGTQFLRHV PFREMVLKLG RTLESSGSFQ NDCHLCHTLR 250 DLIGLSIQRN ISKLLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of cDNA encoding human macrophage c-type lectin: its unique carbohydrate binding specificity for Tn antigen." Suzuki N., Yamamoto K., Toyoshima S., Osawa T., Irimura T. J. Immunol. 156:128-135(1996) [PubMed: 8598452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, VARIANT ARG-35. Tissue: Peripheral blood monocyte.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain.

Web resources

Functional Glycomics Gateway - Glycan Binding

MGL

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50532 mRNA. Translation: BAA09101.1. AK292363 mRNA. Translation: BAF85052.1. BC027858 mRNA. Translation: AAH27858.1. BC039011 mRNA. Translation: AAH39011.1.
IPI	IPI00216794. IPI00337367. IPI00448891.
RefSeq	NP_006335.2. NP_878910.1.
UniGene	Hs.54403
3D structure databases
HSSP	HSSP built from PDB template 1DV8 based on UniProtKB P07306.
SMR	Q8IUN9. Positions 181-308.
ModBase	Search...
Proteomic databases
PRIDE	Q8IUN9.
Genome annotation databases
Ensembl	ENSG00000132514. Homo sapiens. [Contig view]
GeneID	10462.
KEGG	hsa:10462.
Organism-specific databases
GeneCards	GC17M006919.
H-InvDB	HIX0027188.
HGNC	HGNC:16916. CLEC10A.
MIM	605999. gene.
PharmGKB	PA134975011.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8IUN9.
HOVERGEN	Q8IUN9.
OMA	Q8IUN9. GSSYTWM.
Gene expression databases
ArrayExpress	Q8IUN9.
Bgee	Q8IUN9.
CleanEx	HS_CLEC10A.
GermOnline	ENSG00000132514. Homo sapiens.
Family and domain databases
InterPro	IPR002353. AntifreezeII. IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR005640. Lectin_N. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
Pfam	PF00059. Lectin_C. 1 hit. PF03954. Lectin_N. 1 hit. [Graphical view]
PRINTS	PR00356. ANTIFREEZEII.
SMART	SM00034. CLECT. 1 hit. [Graphical view]
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39671.
SOURCE	Search...

Entry information

Entry name

CLC10_HUMAN

Accession

Primary (citable) accession number: Q8IUN9
Secondary accession number(s): A8K8J8, Q14538, Q6PIW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)