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Protein

Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA

Gene

MINA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.8 Publications

Catalytic activityi

L-histidine-[60S ribosomal protein L27a] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L27a] + succinate + CO2.1 Publication

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Iron; catalyticCurated1
Metal bindingi181Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi240Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Ribosome biogenesis, Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA (EC:1.14.11.-)
Alternative name(s):
60S ribosomal protein L27a histidine hydroxylase
Histone lysine demethylase MINA
MYC-induced nuclear antigen
Mineral dust-induced gene protein
Nucleolar protein 52
Ribosomal oxygenase MINA
Short name:
ROX
Gene namesi
Name:MINAImported
Synonyms:MDIGImported, MINA53Imported, NO52Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:19441. MINA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179H → Y: Abolishes demethylase activity. 1 Publication1

Organism-specific databases

DisGeNETi84864.
OpenTargetsiENSG00000170854.
PharmGKBiPA134991047.

Polymorphism and mutation databases

BioMutaiMINA.
DMDMi74750624.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003083771 – 465Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINAAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei309PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IUF8.
MaxQBiQ8IUF8.
PaxDbiQ8IUF8.
PeptideAtlasiQ8IUF8.
PRIDEiQ8IUF8.
TopDownProteomicsiQ8IUF8-2. [Q8IUF8-2]

PTM databases

iPTMnetiQ8IUF8.
PhosphoSitePlusiQ8IUF8.

Expressioni

Tissue specificityi

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.4 Publications

Inductioni

Up-regulated in response to MYC, in alveolar macrophages from coal miners and in silica particle-treated A549 lung cancer cells.2 Publications

Gene expression databases

BgeeiENSG00000170854.
CleanExiHS_MINA.
ExpressionAtlasiQ8IUF8. baseline and differential.
GenevisibleiQ8IUF8. HS.

Organism-specific databases

HPAiCAB013458.
HPA007603.
HPA008080.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-751580,EBI-751580

Protein-protein interaction databases

BioGridi124309. 29 interactors.
DIPiDIP-28141N.
IntActiQ8IUF8. 10 interactors.
MINTiMINT-1454329.
STRINGi9606.ENSP00000328251.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 45Combined sources8
Turni46 – 48Combined sources3
Helixi51 – 57Combined sources7
Turni58 – 61Combined sources4
Beta strandi64 – 66Combined sources3
Helixi71 – 80Combined sources10
Helixi85 – 90Combined sources6
Beta strandi94 – 96Combined sources3
Turni97 – 99Combined sources3
Beta strandi100 – 106Combined sources7
Beta strandi109 – 112Combined sources4
Beta strandi116 – 119Combined sources4
Helixi121 – 129Combined sources9
Beta strandi134 – 138Combined sources5
Helixi140 – 142Combined sources3
Helixi145 – 158Combined sources14
Beta strandi162 – 169Combined sources8
Beta strandi178 – 180Combined sources3
Beta strandi182 – 192Combined sources11
Beta strandi194 – 199Combined sources6
Helixi214 – 216Combined sources3
Beta strandi221 – 226Combined sources6
Beta strandi231 – 234Combined sources4
Beta strandi239 – 243Combined sources5
Beta strandi246 – 248Combined sources3
Beta strandi251 – 258Combined sources8
Helixi264 – 276Combined sources13
Helixi284 – 287Combined sources4
Helixi294 – 296Combined sources3
Helixi302 – 321Combined sources20
Helixi329 – 337Combined sources9
Beta strandi344 – 348Combined sources5
Turni349 – 351Combined sources3
Beta strandi363 – 366Combined sources4
Helixi369 – 371Combined sources3
Beta strandi372 – 375Combined sources4
Beta strandi390 – 395Combined sources6
Turni402 – 405Combined sources4
Beta strandi417 – 420Combined sources4
Helixi421 – 423Combined sources3
Helixi424 – 431Combined sources8
Beta strandi434 – 437Combined sources4
Helixi438 – 440Combined sources3
Helixi446 – 458Combined sources13
Beta strandi462 – 464Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XDVX-ray2.57A26-465[»]
4BU2X-ray2.78A26-465[»]
4BXFX-ray2.05A/B26-465[»]
ProteinModelPortaliQ8IUF8.
SMRiQ8IUF8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IUF8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 271JmjCPROSITE-ProRule annotationAdd BLAST133

Sequence similaritiesi

Belongs to the ROX family. MINA53 subfamily.Curated
Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3706. Eukaryota.
ENOG410YNEJ. LUCA.
GeneTreeiENSGT00390000000083.
HOVERGENiHBG055265.
InParanoidiQ8IUF8.
OMAiYQNNSWG.
OrthoDBiEOG091G04K2.
PhylomeDBiQ8IUF8.
TreeFamiTF318659.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 16 Publications (identifier: Q8IUF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK
60 70 80 90 100
TETFFKEFWE QKPLLIQRDD PALATYYGSL FKLTDLKSLC SRGMYYGRDV
110 120 130 140 150
NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI
160 170 180 190 200
QEKLECYFGS LVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH
210 220 230 240 250
PTVPLAREYS VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH
260 270 280 290 300
STHVTISTYQ NNSWGDFLLD TISGLVFDTA KEDVELRTGI PRQLLLQVES
310 320 330 340 350
TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLP PYSAGDGAEL
360 370 380 390 400
STPGGKLPRL DSVVRLQFKD HIVLTVLPDQ DQSDEAQEKM VYIYHSLKNS
410 420 430 440 450
RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD LKLTTDEEKE
460
SLVLSLWTEC LIQVV
Length:465
Mass (Da):52,800
Last modified:March 1, 2003 - v1
Checksum:iB59F8AE9C802FEB0
GO
Isoform 21 Publication (identifier: Q8IUF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-280: SWGDFLLDTISGLVFDTA → DAGARMRRCDLRAIAPQK
     281-465: Missing.

Show »
Length:280
Mass (Da):31,818
Checksum:i8B69C261ABC594EB
GO
Isoform 31 Publication (identifier: Q8IUF8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-254: Missing.
     255-261: TISTYQN → MLLQVPC

Show »
Length:211
Mass (Da):23,909
Checksum:iDA5AB0F080853151
GO
Isoform 4 (identifier: Q8IUF8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-297: Missing.

Show »
Length:464
Mass (Da):52,672
Checksum:i24902143244D1A79
GO

Sequence cautioni

The sequence CAH10679 differs from that shown. Reason: Erroneous termination at position 431. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87K → E in BAC16359 (PubMed:12091391).Curated1
Sequence conflicti107N → S in BAC16359 (PubMed:12091391).Curated1
Sequence conflicti159G → S in BAB55024 (PubMed:14702039).Curated1
Sequence conflicti221V → A in BAC16359 (PubMed:12091391).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05407917A → P.Corresponds to variant rs35391656dbSNPEnsembl.1
Natural variantiVAR_062241201P → L.Corresponds to variant rs56183666dbSNPEnsembl.1
Natural variantiVAR_036811386A → T.4 PublicationsCorresponds to variant rs2172257dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0525871 – 254Missing in isoform 3. 1 PublicationAdd BLAST254
Alternative sequenceiVSP_052588255 – 261TISTYQN → MLLQVPC in isoform 3. 1 Publication7
Alternative sequenceiVSP_052589263 – 280SWGDF…VFDTA → DAGARMRRCDLRAIAPQK in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_052590281 – 465Missing in isoform 2. 1 PublicationAdd BLAST185
Alternative sequenceiVSP_038373297Missing in isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083189 mRNA. Translation: BAC16537.1.
AB083190 mRNA. Translation: BAC16358.1.
AB083191 mRNA. Translation: BAC16359.1.
AB083192 mRNA. Translation: BAC16360.1.
AB083193 mRNA. Translation: BAC16361.1.
AY390536 mRNA. Translation: AAR27293.1.
AY302110 mRNA. Translation: AAP59421.1.
AY456380 mRNA. Translation: AAR21572.1.
DQ453796 mRNA. Translation: ABE28016.1.
AK027299 mRNA. Translation: BAB55024.1.
AC026100 Genomic DNA. No translation available.
AC110491 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79872.1.
CH471052 Genomic DNA. Translation: EAW79873.1.
BC014928 mRNA. Translation: AAH14928.1.
CR627479 mRNA. Translation: CAH10679.1. Different termination.
CCDSiCCDS2929.1. [Q8IUF8-4]
CCDS43114.1. [Q8IUF8-1]
RefSeqiNP_001035998.1. NM_001042533.2. [Q8IUF8-1]
NP_001248758.1. NM_001261829.1. [Q8IUF8-4]
NP_116167.3. NM_032778.5. [Q8IUF8-4]
NP_694822.2. NM_153182.3. [Q8IUF8-1]
XP_005247895.1. XM_005247838.4. [Q8IUF8-1]
UniGeneiHs.570562.
Hs.742206.

Genome annotation databases

EnsembliENST00000333396; ENSP00000328251; ENSG00000170854. [Q8IUF8-1]
ENST00000360258; ENSP00000353395; ENSG00000170854. [Q8IUF8-4]
ENST00000394198; ENSP00000377748; ENSG00000170854. [Q8IUF8-1]
ENST00000514314; ENSP00000424955; ENSG00000170854. [Q8IUF8-2]
GeneIDi84864.
KEGGihsa:84864.
UCSCiuc003drz.3. human. [Q8IUF8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083189 mRNA. Translation: BAC16537.1.
AB083190 mRNA. Translation: BAC16358.1.
AB083191 mRNA. Translation: BAC16359.1.
AB083192 mRNA. Translation: BAC16360.1.
AB083193 mRNA. Translation: BAC16361.1.
AY390536 mRNA. Translation: AAR27293.1.
AY302110 mRNA. Translation: AAP59421.1.
AY456380 mRNA. Translation: AAR21572.1.
DQ453796 mRNA. Translation: ABE28016.1.
AK027299 mRNA. Translation: BAB55024.1.
AC026100 Genomic DNA. No translation available.
AC110491 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79872.1.
CH471052 Genomic DNA. Translation: EAW79873.1.
BC014928 mRNA. Translation: AAH14928.1.
CR627479 mRNA. Translation: CAH10679.1. Different termination.
CCDSiCCDS2929.1. [Q8IUF8-4]
CCDS43114.1. [Q8IUF8-1]
RefSeqiNP_001035998.1. NM_001042533.2. [Q8IUF8-1]
NP_001248758.1. NM_001261829.1. [Q8IUF8-4]
NP_116167.3. NM_032778.5. [Q8IUF8-4]
NP_694822.2. NM_153182.3. [Q8IUF8-1]
XP_005247895.1. XM_005247838.4. [Q8IUF8-1]
UniGeneiHs.570562.
Hs.742206.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XDVX-ray2.57A26-465[»]
4BU2X-ray2.78A26-465[»]
4BXFX-ray2.05A/B26-465[»]
ProteinModelPortaliQ8IUF8.
SMRiQ8IUF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124309. 29 interactors.
DIPiDIP-28141N.
IntActiQ8IUF8. 10 interactors.
MINTiMINT-1454329.
STRINGi9606.ENSP00000328251.

PTM databases

iPTMnetiQ8IUF8.
PhosphoSitePlusiQ8IUF8.

Polymorphism and mutation databases

BioMutaiMINA.
DMDMi74750624.

Proteomic databases

EPDiQ8IUF8.
MaxQBiQ8IUF8.
PaxDbiQ8IUF8.
PeptideAtlasiQ8IUF8.
PRIDEiQ8IUF8.
TopDownProteomicsiQ8IUF8-2. [Q8IUF8-2]

Protocols and materials databases

DNASUi84864.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333396; ENSP00000328251; ENSG00000170854. [Q8IUF8-1]
ENST00000360258; ENSP00000353395; ENSG00000170854. [Q8IUF8-4]
ENST00000394198; ENSP00000377748; ENSG00000170854. [Q8IUF8-1]
ENST00000514314; ENSP00000424955; ENSG00000170854. [Q8IUF8-2]
GeneIDi84864.
KEGGihsa:84864.
UCSCiuc003drz.3. human. [Q8IUF8-1]

Organism-specific databases

CTDi84864.
DisGeNETi84864.
GeneCardsiMINA.
HGNCiHGNC:19441. MINA.
HPAiCAB013458.
HPA007603.
HPA008080.
MIMi612049. gene.
neXtProtiNX_Q8IUF8.
OpenTargetsiENSG00000170854.
PharmGKBiPA134991047.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3706. Eukaryota.
ENOG410YNEJ. LUCA.
GeneTreeiENSGT00390000000083.
HOVERGENiHBG055265.
InParanoidiQ8IUF8.
OMAiYQNNSWG.
OrthoDBiEOG091G04K2.
PhylomeDBiQ8IUF8.
TreeFamiTF318659.

Miscellaneous databases

EvolutionaryTraceiQ8IUF8.
GeneWikiiMINA.
MYC-induced_nuclear_antigen.
GenomeRNAii84864.
PROiQ8IUF8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170854.
CleanExiHS_MINA.
ExpressionAtlasiQ8IUF8. baseline and differential.
GenevisibleiQ8IUF8. HS.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMINA_HUMAN
AccessioniPrimary (citable) accession number: Q8IUF8
Secondary accession number(s): D3DN35
, Q6AHW4, Q6SKS0, Q8IU69, Q8IUF6, Q8IUF7, Q96C17, Q96KB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.