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Protein

Rhophilin-2

Gene

RHPN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity.1 Publication

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
SignaLinkiQ8IUC4.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhophilin-2
Alternative name(s):
76 kDa RhoB effector protein
GTP-Rho-binding protein 2
p76RBE
Gene namesi
Name:RHPN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:19974. RHPN2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58 – 59EN → AA: Abolishes interaction with RhoA. 1 Publication2
Mutagenesisi518R → A: Does not induce actin disassembly but still interacts with RhoA; when associated with A-526 and A-527. 1 Publication1
Mutagenesisi526 – 527LG → AA: Does not induce actin disassembly but still interacts with RhoA; when associated with A-518. 1 Publication2

Organism-specific databases

DisGeNETi85415.
OpenTargetsiENSG00000131941.
PharmGKBiPA134979284.

Polymorphism and mutation databases

BioMutaiRHPN2.
DMDMi62288912.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188981 – 686Rhophilin-2Add BLAST686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei655PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IUC4.
MaxQBiQ8IUC4.
PaxDbiQ8IUC4.
PeptideAtlasiQ8IUC4.
PRIDEiQ8IUC4.

PTM databases

iPTMnetiQ8IUC4.
PhosphoSitePlusiQ8IUC4.
SwissPalmiQ8IUC4.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in prostate, trachea, stomach, colon, thyroid and pancreas. Expressed at lower level in brain, spinal cord, kidney, placenta and liver.2 Publications

Inductioni

By thyrotropin (TSH). Regulated by the cAMP pathway.1 Publication

Gene expression databases

BgeeiENSG00000131941.
CleanExiHS_RHPN2.
ExpressionAtlasiQ8IUC4. baseline and differential.
GenevisibleiQ8IUC4. HS.

Organism-specific databases

HPAiHPA051749.

Interactioni

Subunit structurei

Interacts with GTP-bound RhoA and RhoB. Interacts with both GTP- and GDP-bound RhoA. According to PubMed:12473120, it does not interact with RhoA. Interacts with KRT18.2 Publications

Protein-protein interaction databases

BioGridi124521. 7 interactors.
IntActiQ8IUC4. 2 interactors.
MINTiMINT-3975222.
STRINGi9606.ENSP00000254260.

Structurei

Secondary structure

1686
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi515 – 519Combined sources5
Helixi522 – 524Combined sources3
Beta strandi528 – 535Combined sources8
Beta strandi537 – 541Combined sources5
Helixi546 – 549Combined sources4
Beta strandi557 – 561Combined sources5
Helixi571 – 579Combined sources9
Turni580 – 583Combined sources4
Beta strandi586 – 592Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VSVX-ray1.82A/B514-595[»]
ProteinModelPortaliQ8IUC4.
SMRiQ8IUC4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IUC4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 110REMAdd BLAST73
Domaini111 – 460BRO1PROSITE-ProRule annotationAdd BLAST350
Domaini515 – 593PDZAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 66Interaction with RhoAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi623 – 626Poly-Asp4

Sequence similaritiesi

Belongs to the RHPN family.Curated
Contains 1 BRO1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.Curated
Contains 1 REM (Hr1) repeat.Curated

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOGENOMiHOG000046725.
HOVERGENiHBG067197.
InParanoidiQ8IUC4.
OMAiHYSEDSY.
OrthoDBiEOG091G04BO.
PhylomeDBiQ8IUC4.
TreeFamiTF323502.

Family and domain databases

Gene3Di1.25.40.280. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR004328. BRO1_dom.
IPR011072. HR1_rho-bd.
IPR001478. PDZ.
[Graphical view]
PfamiPF03097. BRO1. 1 hit.
PF02185. HR1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
SM00742. Hr1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IUC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDALLPAAP QPLEKENDGY FRKGCNPLAQ TGRSKLQNQR AALNQQILKA
60 70 80 90 100
VRMRTGAENL LKVATNSKVR EQVRLELSFV NSDLQMLKEE LEGLNISVGV
110 120 130 140 150
YQNTEEAFTI PLIPLGLKET KDVDFAVVLK DFILEHYSED GYLYEDEIAD
160 170 180 190 200
LMDLRQACRT PSRDEAGVEL LMTYFIQLGF VESRFFPPTR QMGLLFTWYD
210 220 230 240 250
SLTGVPVSQQ NLLLEKASVL FNTGALYTQI GTRCDRQTQA GLESAIDAFQ
260 270 280 290 300
RAAGVLNYLK DTFTHTPSYD MSPAMLSVLV KMMLAQAQES VFEKISLPGI
310 320 330 340 350
RNEFFMLVKV AQEAAKVGEV YQQLHAAMSQ APVKENIPYS WASLACVKAH
360 370 380 390 400
HYAALAHYFT AILLIDHQVK PGTDLDHQEK CLSQLYDHMP EGLTPLATLK
410 420 430 440 450
NDQQRRQLGK SHLRRAMAHH EESVREASLC KKLRSIEVLQ KVLCAAQERS
460 470 480 490 500
RLTYAQHQEE DDLLNLIDAP SVVAKTEQEV DIILPQFSKL TVTDFFQKLG
510 520 530 540 550
PLSVFSANKR WTPPRSIRFT AEEGDLGFTL RGNAPVQVHF LDPYCSASVA
560 570 580 590 600
GAREGDYIVS IQLVDCKWLT LSEVMKLLKS FGEDEIEMKV VSLLDSTSSM
610 620 630 640 650
HNKSATYSVG MQKTYSMICL AIDDDDKTDK TKKISKKLSF LSWGTNKNRQ
660 670 680
KSASTLCLPS VGAARPQVKK KLPSPFSLLN SDSSWY
Length:686
Mass (Da):76,993
Last modified:March 1, 2003 - v1
Checksum:i8D6CA2F9B0405A00
GO
Isoform 2 (identifier: Q8IUC4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.

Note: No experimental confirmation available.
Show »
Length:535
Mass (Da):59,998
Checksum:i259B8CA5A18252C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16E → K in AAH36447 (PubMed:15489334).Curated1
Sequence conflicti51V → M in BAG53600 (PubMed:14702039).Curated1
Sequence conflicti179G → D in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti207 – 214VSQQNLLL → EPAEPGA in AAK58588 (PubMed:12221077).Curated8
Sequence conflicti239Q → E in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti261D → VH in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti290S → C in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti326A → Q in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti340S → L in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti391E → V in BAG37565 (PubMed:14702039).Curated1
Sequence conflicti392G → V in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti412 – 418HLRRAMA → TCADHG in AAK58588 (PubMed:12221077).Curated7
Sequence conflicti417M → T in BAC04471 (Ref. 9) Curated1
Sequence conflicti435S → T in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti504V → A in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti513P → A in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti549V → L in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti553R → K in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti563L → G in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti571L → V in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti583E → R in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti598S → P in BAC04471 (Ref. 9) Curated1
Sequence conflicti600M → T in BAG37565 (PubMed:14702039).Curated1
Sequence conflicti626D → N in AAK58588 (PubMed:12221077).Curated1
Sequence conflicti646N → T in AAK58588 (PubMed:12221077).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06199670R → Q.1 PublicationCorresponds to variant rs28626308dbSNPEnsembl.1
Natural variantiVAR_061997342A → P.Corresponds to variant rs28407794dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0555481 – 151Missing in isoform 2. 1 PublicationAdd BLAST151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347750 mRNA. Translation: CAC87939.1.
AF268032 mRNA. Translation: AAK58588.1.
AF423421 mRNA. Translation: AAQ04062.1.
AK095001 mRNA. Translation: BAC04471.1.
AK098246 mRNA. Translation: BAG53600.1.
AK300775 mRNA. Translation: BAG62439.1.
AK315105 mRNA. Translation: BAG37565.1.
AC008521 Genomic DNA. No translation available.
AC011449 Genomic DNA. No translation available.
AC093069 Genomic DNA. No translation available.
BC036447 mRNA. Translation: AAH36447.1.
AL831950 mRNA. Translation: CAD38597.1.
CCDSiCCDS12427.1. [Q8IUC4-1]
RefSeqiNP_149094.3. NM_033103.4. [Q8IUC4-1]
UniGeneiHs.466435.

Genome annotation databases

EnsembliENST00000254260; ENSP00000254260; ENSG00000131941. [Q8IUC4-1]
GeneIDi85415.
KEGGihsa:85415.
UCSCiuc002nuf.4. human. [Q8IUC4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347750 mRNA. Translation: CAC87939.1.
AF268032 mRNA. Translation: AAK58588.1.
AF423421 mRNA. Translation: AAQ04062.1.
AK095001 mRNA. Translation: BAC04471.1.
AK098246 mRNA. Translation: BAG53600.1.
AK300775 mRNA. Translation: BAG62439.1.
AK315105 mRNA. Translation: BAG37565.1.
AC008521 Genomic DNA. No translation available.
AC011449 Genomic DNA. No translation available.
AC093069 Genomic DNA. No translation available.
BC036447 mRNA. Translation: AAH36447.1.
AL831950 mRNA. Translation: CAD38597.1.
CCDSiCCDS12427.1. [Q8IUC4-1]
RefSeqiNP_149094.3. NM_033103.4. [Q8IUC4-1]
UniGeneiHs.466435.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VSVX-ray1.82A/B514-595[»]
ProteinModelPortaliQ8IUC4.
SMRiQ8IUC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124521. 7 interactors.
IntActiQ8IUC4. 2 interactors.
MINTiMINT-3975222.
STRINGi9606.ENSP00000254260.

PTM databases

iPTMnetiQ8IUC4.
PhosphoSitePlusiQ8IUC4.
SwissPalmiQ8IUC4.

Polymorphism and mutation databases

BioMutaiRHPN2.
DMDMi62288912.

Proteomic databases

EPDiQ8IUC4.
MaxQBiQ8IUC4.
PaxDbiQ8IUC4.
PeptideAtlasiQ8IUC4.
PRIDEiQ8IUC4.

Protocols and materials databases

DNASUi85415.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254260; ENSP00000254260; ENSG00000131941. [Q8IUC4-1]
GeneIDi85415.
KEGGihsa:85415.
UCSCiuc002nuf.4. human. [Q8IUC4-1]

Organism-specific databases

CTDi85415.
DisGeNETi85415.
GeneCardsiRHPN2.
HGNCiHGNC:19974. RHPN2.
HPAiHPA051749.
neXtProtiNX_Q8IUC4.
OpenTargetsiENSG00000131941.
PharmGKBiPA134979284.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOGENOMiHOG000046725.
HOVERGENiHBG067197.
InParanoidiQ8IUC4.
OMAiHYSEDSY.
OrthoDBiEOG091G04BO.
PhylomeDBiQ8IUC4.
TreeFamiTF323502.

Enzyme and pathway databases

ReactomeiR-HSA-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
SignaLinkiQ8IUC4.

Miscellaneous databases

ChiTaRSiRHPN2. human.
EvolutionaryTraceiQ8IUC4.
GeneWikiiRHPN2.
GenomeRNAii85415.
PROiQ8IUC4.

Gene expression databases

BgeeiENSG00000131941.
CleanExiHS_RHPN2.
ExpressionAtlasiQ8IUC4. baseline and differential.
GenevisibleiQ8IUC4. HS.

Family and domain databases

Gene3Di1.25.40.280. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR004328. BRO1_dom.
IPR011072. HR1_rho-bd.
IPR001478. PDZ.
[Graphical view]
PfamiPF03097. BRO1. 1 hit.
PF02185. HR1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
SM00742. Hr1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHPN2_HUMAN
AccessioniPrimary (citable) accession number: Q8IUC4
Secondary accession number(s): B2RCG8
, B3KUY8, B4DUS7, Q8N3T7, Q8N9D6, Q8NE33, Q96RU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.