Q8ISF8 (RFP_ENTQU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Red fluorescent protein eqFP611 Alternative name(s): GFP-like chromoprotein
Organism	Entacmea quadricolor (Bubble-tip anemone) (Parasicyonis actinostoloides)
Taxonomic identifier	6118 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Nynantheae › Actiniidae › Entacmaea

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pigment protein. Ref.1
Subunit structure	Monomer. Ref.1
Post-translational modification	Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use	Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Miscellaneous	The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization. Ref.1
Sequence similarities	Belongs to the GFP family. Ref.1
Biophysicochemical properties	Absorption: Abs(max)=559 nm Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm.

Ontologies

Keywords
Biological process	Luminescence
Ligand	Chromophore
Molecular function	Photoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 231

231

Red fluorescent protein eqFP611

PRO_0000192578

Amino acid modifications

Modified residue

64

1

(E)-2,3-didehydrotyrosine UniProtKB Q9U6Y8

Cross-link

63 ↔ 65

2-iminomethyl-5-imidazolinone (Met-Gly) Ref.2

Secondary structure

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231

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

7 – 19

13

Beta strand

22 – 33

12

Turn

34 – 37

4

Beta strand

38 – 48

11

Helix

55 – 61

7

Turn

73 – 75

3

Helix

81 – 83

3

Turn

84 – 86

3

Beta strand

88 – 96

9

Beta strand

101 – 111

11

Beta strand

114 – 124

11

Turn

131 – 135

5

Beta strand

137 – 140

4

Beta strand

143 – 150

8

Beta strand

153 – 164

12

Turn

165 – 167

3

Beta strand

169 – 182

14

Helix

184 – 186

3

Beta strand

192 – 205

14

Turn

206 – 209

4

Beta strand

210 – 220

11

Beta strand

227 – 229

3

Sequences

	Sequence		Length	Mass (Da)
	Q8ISF8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 86467A7EB5D6DD60	FASTA	231	26,053
	10 20 30 40 50 60 MNSLIKENMR MMVVMEGSVN GYQFKCTGEG DGNPYMGTQT MRIKVVEGGP LPFAFDILAT 70 80 90 100 110 120 SFMYGSKTFI KHTKGIPDFF KQSFPEGFTW ERVTRYEDGG VFTVMQDTSL EDGCLVYHAK 130 140 150 160 170 180 VTGVNFPSNG AVMQKKTKGW EPNTEMLYPA DGGLRGYSQM ALNVDGGGYL SCSFETTYRS 190 200 210 220 230 KKTVENFKMP GFHFVDHRLE RLEESDKEMF VVQHEHAVAK FCDLPSKLGR L

References

[1]	"A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria)." Wiedenmann J., Schenk A., Roecker C., Girod A., Spindler K.-D., Nienhaus G.U. Proc. Natl. Acad. Sci. U.S.A. 99:11646-11651(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
[2]	"The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor." Petersen J., Wilmann P.G., Beddoe T., Oakley A.J., Devenish R.J., Prescott M., Rossjohn J. J. Biol. Chem. 278:44626-44631(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY130757 mRNA. Translation: AAN05449.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UIS	X-ray	2.00	A/B	1-231	[»]
3E5T	X-ray	1.10	A	1-231	[»]
3E5V	X-ray	2.10	A	1-231	[»]
3E5W	X-ray	1.71	A/B/C/D	1-231	[»]
3IP2	X-ray	1.60	A	4-229	[»]
3U0L	X-ray	1.25	A	1-221	[»]
3U0M	X-ray	1.65	A	1-221	[»]
3U0N	X-ray	1.60	A	1-221	[»]

ProteinModelPortal

Q8ISF8.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.40.155.10. 1 hit.

InterPro

IPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
[Graphical view]

Pfam

PF01353. GFP. 1 hit.
[Graphical view]

SUPFAM

SSF54511. GFP_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8ISF8.

Entry information

Entry name

RFP_ENTQU

Accession

Primary (citable) accession number: Q8ISF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)