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Q8ISF8 (RFP_ENTQU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Red fluorescent protein eqFP611
Alternative name(s):
GFP-like chromoprotein
OrganismEntacmaea quadricolor (Bubble-tip anemone) (Parasicyonis actinostoloides)
Taxonomic identifier6118 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaNynantheaeActiniidaeEntacmaea

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pigment protein. Ref.1

Subunit structure

Monomer. Ref.1

Post-translational modification

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.

Biotechnological use

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Miscellaneous

The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization. Ref.1

Sequence similarities

Belongs to the GFP family. Ref.1

Biophysicochemical properties

Absorption:

Abs(max)=559 nm

Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm.

Ontologies

Keywords
   Biological processLuminescence
   LigandChromophore
   Molecular functionPhotoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

protein-chromophore linkage

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231Red fluorescent protein eqFP611
PRO_0000192578

Amino acid modifications

Modified residue641(E)-2,3-didehydrotyrosine UniProtKB Q9U6Y8
Cross-link63 ↔ 652-iminomethyl-5-imidazolinone (Met-Gly) Ref.2

Secondary structure

......................................... 231
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8ISF8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 86467A7EB5D6DD60

FASTA23126,053
        10         20         30         40         50         60 
MNSLIKENMR MMVVMEGSVN GYQFKCTGEG DGNPYMGTQT MRIKVVEGGP LPFAFDILAT 

        70         80         90        100        110        120 
SFMYGSKTFI KHTKGIPDFF KQSFPEGFTW ERVTRYEDGG VFTVMQDTSL EDGCLVYHAK 

       130        140        150        160        170        180 
VTGVNFPSNG AVMQKKTKGW EPNTEMLYPA DGGLRGYSQM ALNVDGGGYL SCSFETTYRS 

       190        200        210        220        230 
KKTVENFKMP GFHFVDHRLE RLEESDKEMF VVQHEHAVAK FCDLPSKLGR L 

« Hide

References

[1]"A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria)."
Wiedenmann J., Schenk A., Roecker C., Girod A., Spindler K.-D., Nienhaus G.U.
Proc. Natl. Acad. Sci. U.S.A. 99:11646-11651(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
[2]"The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor."
Petersen J., Wilmann P.G., Beddoe T., Oakley A.J., Devenish R.J., Prescott M., Rossjohn J.
J. Biol. Chem. 278:44626-44631(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY130757 mRNA. Translation: AAN05449.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UISX-ray2.00A/B1-231[»]
3E5TX-ray1.10A1-231[»]
3E5VX-ray2.10A1-231[»]
3E5WX-ray1.71A/B/C/D1-231[»]
3IP2X-ray1.60A4-229[»]
3U0LX-ray1.25A1-221[»]
3U0MX-ray1.65A1-221[»]
3U0NX-ray1.60A1-221[»]
ProteinModelPortalQ8ISF8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.155.10. 1 hit.
InterProIPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
[Graphical view]
PfamPF01353. GFP. 1 hit.
[Graphical view]
SUPFAMSSF54511. SSF54511. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ8ISF8.

Entry information

Entry nameRFP_ENTQU
AccessionPrimary (citable) accession number: Q8ISF8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: March 1, 2003
Last modified: December 11, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references