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Q8ISF8

- RFP_ENTQU

UniProt

Q8ISF8 - RFP_ENTQU

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Protein

Red fluorescent protein eqFP611

Gene
N/A
Organism
Entacmaea quadricolor (Bubble-tip anemone) (Parasicyonis actinostoloides)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Pigment protein.1 Publication

Absorptioni

Abs(max)=559 nm

Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm.

GO - Biological processi

  1. bioluminescence Source: UniProtKB-KW
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Red fluorescent protein eqFP611
Alternative name(s):
GFP-like chromoprotein
OrganismiEntacmaea quadricolor (Bubble-tip anemone) (Parasicyonis actinostoloides)
Taxonomic identifieri6118 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaNynantheaeActiniidaeEntacmaea

Pathology & Biotechi

Biotechnological usei

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Curated

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Red fluorescent protein eqFP611PRO_0000192578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki63 ↔ 652-iminomethyl-5-imidazolinone (Met-Gly)1 Publication
Modified residuei64 – 641(E)-2,3-didehydrotyrosine

Post-translational modificationi

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
231
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Beta strandi7 – 1913
Beta strandi22 – 3312
Turni34 – 374
Beta strandi38 – 4811
Helixi55 – 617
Turni73 – 753
Helixi81 – 833
Turni84 – 863
Beta strandi88 – 969
Beta strandi101 – 11111
Beta strandi114 – 12411
Turni131 – 1355
Beta strandi137 – 1404
Beta strandi143 – 1508
Beta strandi153 – 16412
Turni165 – 1673
Beta strandi169 – 18214
Helixi184 – 1863
Beta strandi192 – 20514
Turni206 – 2094
Beta strandi210 – 22011
Beta strandi227 – 2293

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UISX-ray2.00A/B1-231[»]
3E5TX-ray1.10A1-231[»]
3E5VX-ray2.10A1-231[»]
3E5WX-ray1.71A/B/C/D1-231[»]
3IP2X-ray1.60A34-229[»]
3U0LX-ray1.25A1-224[»]
3U0MX-ray1.65A1-224[»]
3U0NX-ray1.60A1-224[»]
ProteinModelPortaliQ8ISF8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ISF8.

Family & Domainsi

Sequence similaritiesi

Belongs to the GFP family.1 Publication

Family and domain databases

Gene3Di2.40.155.10. 1 hit.
InterProiIPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
[Graphical view]
PfamiPF01353. GFP. 1 hit.
[Graphical view]
SUPFAMiSSF54511. SSF54511. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ISF8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSLIKENMR MMVVMEGSVN GYQFKCTGEG DGNPYMGTQT MRIKVVEGGP
60 70 80 90 100
LPFAFDILAT SFMYGSKTFI KHTKGIPDFF KQSFPEGFTW ERVTRYEDGG
110 120 130 140 150
VFTVMQDTSL EDGCLVYHAK VTGVNFPSNG AVMQKKTKGW EPNTEMLYPA
160 170 180 190 200
DGGLRGYSQM ALNVDGGGYL SCSFETTYRS KKTVENFKMP GFHFVDHRLE
210 220 230
RLEESDKEMF VVQHEHAVAK FCDLPSKLGR L
Length:231
Mass (Da):26,053
Last modified:March 1, 2003 - v1
Checksum:i86467A7EB5D6DD60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130757 mRNA. Translation: AAN05449.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130757 mRNA. Translation: AAN05449.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UIS X-ray 2.00 A/B 1-231 [» ]
3E5T X-ray 1.10 A 1-231 [» ]
3E5V X-ray 2.10 A 1-231 [» ]
3E5W X-ray 1.71 A/B/C/D 1-231 [» ]
3IP2 X-ray 1.60 A 34-229 [» ]
3U0L X-ray 1.25 A 1-224 [» ]
3U0M X-ray 1.65 A 1-224 [» ]
3U0N X-ray 1.60 A 1-224 [» ]
ProteinModelPortali Q8ISF8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q8ISF8.

Family and domain databases

Gene3Di 2.40.155.10. 1 hit.
InterProi IPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
[Graphical view ]
Pfami PF01353. GFP. 1 hit.
[Graphical view ]
SUPFAMi SSF54511. SSF54511. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria)."
    Wiedenmann J., Schenk A., Roecker C., Girod A., Spindler K.-D., Nienhaus G.U.
    Proc. Natl. Acad. Sci. U.S.A. 99:11646-11651(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
  2. "The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor."
    Petersen J., Wilmann P.G., Beddoe T., Oakley A.J., Devenish R.J., Prescott M., Rossjohn J.
    J. Biol. Chem. 278:44626-44631(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiRFP_ENTQU
AccessioniPrimary (citable) accession number: Q8ISF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization.1 Publication

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3