Q8ISC9 (CSW_DROSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase corkscrew
EC=3.1.3.48

Gene names

Name:

csw

Organism

Drosophila simulans (Fruit fly)

Taxonomic identifier

7240 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	555 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required in all receptor tyrosine kinase signaling pathways. Functions downstream of the receptor tyrosine kinase torso, acting in concert with D-Raf via tailless. Also functions downstream of Egfr (epidermal growth factor receptor) and btl (fibroblast growth factor receptor). The SH2 domain suggests that csw effects its role by mediating heteromeric protein interactions. Maternally required for normal determination of cell fates at the termini of the embryo. Required for cell fate specification of the ventral ectoderm, in the developing embryonic CNS and for embryonic tracheal cell migration. Functions during imaginal development for proper formation of adult structures such as eyes, aristae, L5 wing vein and the tarsal claw By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Developmental protein Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC terminal region determination Inferred from sequence or structural similarity. Source: UniProtKB torso signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 555	›555	Tyrosine-protein phosphatase corkscrew		PRO_0000094851
Regions
	Domain	85 – 355	271	Tyrosine-protein phosphatase
	Region	11 – 103	93	PTPase insert (Cys/Ser-rich)
	Region	293 – 299	7	Substrate binding By similarity
	Compositional bias	447 – 481	35	Asn-rich
	Compositional bias	507 – 510	4	Poly-Gln
Sites
	Active site	293	1	Phosphocysteine intermediate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	340	1	Substrate By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q8ISC9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 801534C03AE9DCFD	FASTA	555	59,841
	10 20 30 40 50 60 LPTDGDLYNM SSSSESLNSS VPSCPACTAA QTQRNCSNCQ LMNKTCVQCA VKSAILPYSN 70 80 90 100 110 120 CATCSRKSDS LSKHKRSESS ASSSPSSGSG SGPGSSGTSG VSSVNGPGTP TNLTSGTAGC 130 140 150 160 170 180 LAGLLKRHSN DSSGAVSISM AERERERERE MFKTYIATQG CLLTQQVNTV TDFWNMVWQE 190 200 210 220 230 240 NTRVIVMTTK EYERGKEKCA RYWPDEGRSE QFGHARIQCV SENSTSDYTL REFLVSWRDQ 250 260 270 280 290 300 PARRIFHYHF QVWPDHGVPA DPGCVLNFLQ DVNTRQSHLA QAGEKPGPIC VHCSAGIGRT 310 320 330 340 350 360 GTFIVIDMIL DQIVRNGLDT EIDIQRTIQM VRSQRSGLVQ TEAQYKFVYY AVQHYIQTLI 370 380 390 400 410 420 ARKRAEEQSL QVGREYTNIK YTGEIGNDSQ RSPLPPAISS ISLVPSKTPL TPTSADLGTG 430 440 450 460 470 480 MGLSMGVGMG VGNKHASKQQ PPLPVVNCNN NNNGIGNSSC SNGGGSSNTS SSNGSSNGNI 490 500 510 520 530 540 NALLGGIGLG LGGNMRKSNF YSDSLKQQQQ REEQAPAGAG KMQQPAPPLR PRPGILKLLT 550 SPVIFQQNSK TFPKT

References

[1]	"Contrasting selection pressures on components of the Ras-mediated signal transduction pathway in Drosophila." Riley R.M., Jin W., Gibson G. Mol. Ecol. 12:1315-1323(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SIM31.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY135142 Genomic DNA. Translation: AAN17645.1.
3D structure databases
ProteinModelPortal	Q8ISC9.
SMR	Q8ISC9. Positions 152-360.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0063985. Dsim\csw.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSW_DROSI

Accession

Primary (citable) accession number: Q8ISC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project