Q8ISB6 (BGBP2_MANSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-1,3-glucan-binding protein 2 Short name=BGBP-2 Alternative name(s): Beta-1,3-glucan recognition protein 2 Short name=BetaGRP-2
Organism	Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifier	7130 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Sphingidae › Sphinginae › Sphingini › Manduca

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and lipoteichoic acid and causes aggregation of invading micro-organisms. Binding to beta-1,3-glucan activates the phenoloxidase cascade. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Cuticle and fat body. Ref.1
Developmental stage	Expression levels are low during larval feeding stages and increase dramatically at the start of the wandering stage. Ref.1
Induction	By bacterial and yeast infection. Ref.1
Post-translational modification	N-glycosylated. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.
Mass spectrometry	Molecular mass is 54920 Da from positions 19 - 482. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	bacterial cell surface binding Inferred from direct assay Ref.1. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential Ref.1
	Chain	19 – 482	464	Beta-1,3-glucan-binding protein 2 Ref.1		PRO_0000002822
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ISB6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 233755F7AD37E8A2	FASTA	482	54,414
	10 20 30 40 50 60 MWIKSVCLFA TIAGCLGQRG GPYKVPDAKL EAIYPKGLRV SVPDDGYSLF AFHGKLNEEM 70 80 90 100 110 120 EGLEAGHWSR DITKAKQGRW IFRDRNAELK LGDKIYFWTY VIKDGLGYRQ DNGEWTVTEF 130 140 150 160 170 180 VNENGTVVDT STAPPPVAPA VSEEDQSPGP QWRPCERSLT ESLARERVCK GSLVFSEDFD 190 200 210 220 230 240 GSSLADLGNW TAEVRFPGEP DYPYNLYTTD GTVGFESGSL VVRPVMTESK YHEGIIYDRL 250 260 270 280 290 300 DLERCTGQLG TLECRRESSG GQIVPPVMTA KLATRRSFAF KFGRIDIKAK MPRGDWLIPE 310 320 330 340 350 360 LNLEPLDNIY GNQRYASGLM RVAFVRGNDV YAKKLYGGPI MSDADPFRSM LLKDKQGLAN 370 380 390 400 410 420 WNNDYHVYSL LWKPNGLELM VDGEVYGTID AGDGFYQIAK NNLVSHASQW LKGTVMAPFD 430 440 450 460 470 480 EKFFITLGLR VAGIHDFTDG PGKPWENKGT KAMINFWNNR FRWFPTWHDT SLKVDYVRVY AL

References

[1]

"Beta-1,3-glucan recognition protein-2 (betaGRP-2) from Manduca sexta: an acute-phase protein that binds beta-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria and stimulate prophenoloxidase activation." Jiang H., Ma C., Lu Z.-Q., Kanost M.R. Insect Biochem. Mol. Biol. 34:89-100(2004) [PubMed: 14976985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-45, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, GLYCOSYLATION, MASS SPECTROMETRY. Tissue: Cuticle and Fat body.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY135522 mRNA. Translation: AAN10151.1.
3D structure databases
ProteinModelPortal	Q8ISB6.
ModBase	Search...
Protein family/group databases
CAZy	CBM39. Carbohydrate-Binding Module Family 39. GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGBP2_MANSE

Accession

Primary (citable) accession number: Q8ISB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	March 1, 2003
Last modified:	January 11, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)