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Protein

cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F

Gene

dnc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Vital for female fertility. Required for learning/memory (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Pathwayi: 3',5'-cyclic AMP degradation

This protein is involved in step 1 of the subpathway that synthesizes AMP from 3',5'-cyclic AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I (dnc), cAMP-specific 3',5'-cyclic phosphodiesterase (dnc), High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8 (Pde8), cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F (dnc), cAMP-specific 3',5'-cyclic phosphodiesterase, isoforms N/G (dnc)
This subpathway is part of the pathway 3',5'-cyclic AMP degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from 3',5'-cyclic AMP, the pathway 3',5'-cyclic AMP degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei324Proton donorBy similarity1
Metal bindingi328Divalent metal cation 1By similarity1
Metal bindingi364Divalent metal cation 1By similarity1
Metal bindingi365Divalent metal cation 1By similarity1
Metal bindingi365Divalent metal cation 2By similarity1
Binding sitei365cAMPBy similarity1
Metal bindingi482Divalent metal cation 1By similarity1
Binding sitei482cAMPBy similarity1
Sitei485Binds AMP, but not cAMPBy similarity1
Binding sitei533cAMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi324 – 328cAMPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • anesthesia-resistant memory Source: FlyBase
  • associative learning Source: FlyBase
  • axon extension Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • cAMP catabolic process Source: UniProtKB-UniPathway
  • cAMP-mediated signaling Source: FlyBase
  • chemical synaptic transmission Source: FlyBase
  • circadian rhythm Source: FlyBase
  • conditioned taste aversion Source: FlyBase
  • courtship behavior Source: FlyBase
  • learning Source: FlyBase
  • learning or memory Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • mating behavior Source: FlyBase
  • memory Source: FlyBase
  • nonassociative learning Source: FlyBase
  • olfactory learning Source: FlyBase
  • regulation of protein kinase A signaling Source: FlyBase
  • reproduction Source: FlyBase
  • short-term memory Source: FlyBase
  • thermosensory behavior Source: FlyBase

Keywordsi

Molecular functionHydrolase
LigandcAMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-180024 DARPP-32 events
R-DME-418555 G alpha (s) signalling events
UniPathwayiUPA00762; UER00747

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F (EC:3.1.4.53)
Alternative name(s):
Learning/memory process protein
Protein dunce
Gene namesi
Name:dncImported
ORF Names:CG32498
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000479 dnc

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988201 – 662cAMP-specific 3',5'-cyclic phosphodiesterase, isoform FAdd BLAST662

Proteomic databases

PRIDEiQ8IRU4

Expressioni

Gene expression databases

BgeeiFBgn0000479
ExpressionAtlasiQ8IRU4 baseline and differential
GenevisibleiQ8IRU4 DM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi57834, 15 interactors

Structurei

3D structure databases

ProteinModelPortaliQ8IRU4
SMRiQ8IRU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini248 – 577PDEasePROSITE-ProRule annotationAdd BLAST330

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 661Gly-richAdd BLAST50

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3689 Eukaryota
ENOG410XRI7 LUCA
GeneTreeiENSGT00760000118889
KOiK13293
OrthoDBiEOG091G06CD

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
PRINTSiPR00387 PDIESTERASE1
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform F1 Publication (identifier: Q8IRU4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLNKNSASSQ SLPRVHSFFN MIPSIMQDDL ALTILNDRDN MFSIKSQRSH
60 70 80 90 100
GEDLIVTPFA QILASLRSVR NNLLSLTNVP ASNKSRRPNQ SSSASRSGNP
110 120 130 140 150
PGAPLSQGEE AYTRLATDTI EELDWCLDQL ETIQTHRSVS DMASLKFKRM
160 170 180 190 200
LNKELSHFSE SSRSGNQISE YICSTFLDKQ QEFDLPSLRV EDNPELVAAN
210 220 230 240 250
AAAGQQSAGQ YARSRSPRGP PMSQISGVKR PLSHTNSFTG ERLPTFGVET
260 270 280 290 300
PRENELGTLL GELDTWGIQI FSIGEFSVNR PLTCVAYTIF QSRELLTSLM
310 320 330 340 350
IPPKTFLNFM STLEDHYVKD NPFHNSLHAA DVTQSTNVLL NTPALEGVFT
360 370 380 390 400
PLEVGGALFA ACIHDVDHPG LTNQFLVNSS SELALMYNDE SVLENHHLAV
410 420 430 440 450
AFKLLQNQGC DIFCNMQKKQ RQTLRKMVID IVLSTDMSKH MSLLADLKTM
460 470 480 490 500
VETKKVAGSG VLLLDNYTDR IQVLENLVHC ADLSNPTKPL PLYKRWVALL
510 520 530 540 550
MEEFFLQGDK ERESGMDISP MCDRHNATIE KSQVGFIDYI VHPLWETWAD
560 570 580 590 600
LVHPDAQDIL DTLEENRDYY QSMIPPSPPP SGVDENPQED RIRFQVTLEE
610 620 630 640 650
SDQENLAELE EGDESGGEST TTGTTGTTAA SALSGAGGGG GGGGGMAPRT
660
GGCQNQPQHG GM
Note: No experimental confirmation available.Curated
Length:662
Mass (Da):72,954
Last modified:March 1, 2003 - v1
Checksum:iD195C7DAFCBD3CC3
GO
Isoform G1 Publication (identifier: Q9W4S9-2) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.Curated
Length:814
Mass (Da):89,085
GO
Isoform IBy similarity (identifier: Q9W4T4-1) [UniParc]FASTAAdd to basket
Also known as: B1 Publication, S
The sequence of this isoform can be found in the external entry Q9W4T4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,209
Mass (Da):129,413
GO
Isoform IIBy similarity (identifier: P12252-1) [UniParc]FASTAAdd to basket
Also known as: I1 Publication, J1 Publication
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,070
Mass (Da):115,079
GO
Isoform III (identifier: P12252-7) [UniParc]FASTAAdd to basket
Also known as: E, P
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation at Met-429 of isoform II.
Length:642
Mass (Da):70,625
GO
Isoform IVBy similarity (identifier: P12252-3) [UniParc]FASTAAdd to basket
Also known as: A1 Publication
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:701
Mass (Da):77,193
GO
Isoform VBy similarity (identifier: P12252-4) [UniParc]FASTAAdd to basket
Also known as: C1 Publication
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,057
Mass (Da):113,506
GO
Isoform VIBy similarity (identifier: P12252-5) [UniParc]FASTAAdd to basket
Also known as: D1 Publication
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,068
Mass (Da):114,836
GO
Isoform VIIBy similarity (identifier: P12252-6) [UniParc]FASTAAdd to basket
Also known as: L1 Publication
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:521
Mass (Da):57,358
GO
Isoform N1 Publication (identifier: Q9W4S9-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.Curated
Length:983
Mass (Da):107,341
GO
Isoform R (identifier: P12252-8) [UniParc] [UniParc]FASTAAdd to basket
Also known as: Q
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Length:703
Mass (Da):77,368
GO
Isoform U (identifier: P12252-9) [UniParc]FASTAAdd to basket
Also known as: T
The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Length:715
Mass (Da):78,887
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAN09606.1
RefSeqiNP_525061.2, NM_080322.3 [Q8IRU4-1]
UniGeneiDm.4817

Genome annotation databases

EnsemblMetazoaiFBtr0070521; FBpp0070497; FBgn0000479 [Q8IRU4-1]
GeneIDi31309
KEGGidme:Dmel_CG32498
UCSCiCG32498-RA d. melanogaster [Q8IRU4-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDE4E_DROME
AccessioniPrimary (citable) accession number: Q8IRU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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