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Q8IRU4

- PDE4E_DROME

UniProt

Q8IRU4 - PDE4E_DROME

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Protein

cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F

Gene

dnc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Vital for female fertility. Required for learning/memory (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.By similarity

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei324 – 3241Proton donorBy similarity
Metal bindingi328 – 3281Divalent metal cation 1By similarity
Metal bindingi364 – 3641Divalent metal cation 1By similarity
Metal bindingi365 – 3651Divalent metal cation 1By similarity
Metal bindingi365 – 3651Divalent metal cation 2By similarity
Binding sitei365 – 3651cAMPBy similarity
Metal bindingi482 – 4821Divalent metal cation 1By similarity
Binding sitei482 – 4821cAMPBy similarity
Sitei485 – 4851Binds AMP, but not cAMPBy similarity
Binding sitei533 – 5331cAMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi324 – 3285cAMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. associative learning Source: FlyBase
  2. axon extension Source: FlyBase
  3. behavioral response to ethanol Source: FlyBase
  4. cAMP catabolic process Source: UniProtKB-UniPathway
  5. cAMP-mediated signaling Source: FlyBase
  6. circadian rhythm Source: FlyBase
  7. conditioned taste aversion Source: FlyBase
  8. courtship behavior Source: FlyBase
  9. lateral inhibition Source: FlyBase
  10. learning Source: FlyBase
  11. learning or memory Source: FlyBase
  12. locomotor rhythm Source: FlyBase
  13. mating behavior Source: FlyBase
  14. memory Source: FlyBase
  15. nonassociative learning Source: FlyBase
  16. olfactory learning Source: FlyBase
  17. oogenesis Source: FlyBase
  18. regulation of protein kinase A signaling Source: FlyBase
  19. reproduction Source: FlyBase
  20. short-term memory Source: FlyBase
  21. synaptic transmission Source: FlyBase
  22. thermosensory behavior Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform F (EC:3.1.4.53)
Alternative name(s):
Learning/memory process protein
Protein dunce
Gene namesi
Name:dncImported
ORF Names:CG32498
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000479. dnc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662cAMP-specific 3',5'-cyclic phosphodiesterase, isoform FPRO_0000198820Add
BLAST

Proteomic databases

PRIDEiQ8IRU4.

Expressioni

Gene expression databases

BgeeiQ8IRU4.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi57834. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8IRU4.
SMRiQ8IRU4. Positions 144-178, 253-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi612 – 66150Gly-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000118889.
KOiK13293.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. Align

Isoform F1 Publication (identifier: Q8IRU4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLNKNSASSQ SLPRVHSFFN MIPSIMQDDL ALTILNDRDN MFSIKSQRSH
60 70 80 90 100
GEDLIVTPFA QILASLRSVR NNLLSLTNVP ASNKSRRPNQ SSSASRSGNP
110 120 130 140 150
PGAPLSQGEE AYTRLATDTI EELDWCLDQL ETIQTHRSVS DMASLKFKRM
160 170 180 190 200
LNKELSHFSE SSRSGNQISE YICSTFLDKQ QEFDLPSLRV EDNPELVAAN
210 220 230 240 250
AAAGQQSAGQ YARSRSPRGP PMSQISGVKR PLSHTNSFTG ERLPTFGVET
260 270 280 290 300
PRENELGTLL GELDTWGIQI FSIGEFSVNR PLTCVAYTIF QSRELLTSLM
310 320 330 340 350
IPPKTFLNFM STLEDHYVKD NPFHNSLHAA DVTQSTNVLL NTPALEGVFT
360 370 380 390 400
PLEVGGALFA ACIHDVDHPG LTNQFLVNSS SELALMYNDE SVLENHHLAV
410 420 430 440 450
AFKLLQNQGC DIFCNMQKKQ RQTLRKMVID IVLSTDMSKH MSLLADLKTM
460 470 480 490 500
VETKKVAGSG VLLLDNYTDR IQVLENLVHC ADLSNPTKPL PLYKRWVALL
510 520 530 540 550
MEEFFLQGDK ERESGMDISP MCDRHNATIE KSQVGFIDYI VHPLWETWAD
560 570 580 590 600
LVHPDAQDIL DTLEENRDYY QSMIPPSPPP SGVDENPQED RIRFQVTLEE
610 620 630 640 650
SDQENLAELE EGDESGGEST TTGTTGTTAA SALSGAGGGG GGGGGMAPRT
660
GGCQNQPQHG GM

Note: No experimental confirmation available.Curated

Length:662
Mass (Da):72,954
Last modified:March 1, 2003 - v1
Checksum:iD195C7DAFCBD3CC3
GO
Isoform G1 Publication (identifier: Q9W4S9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.Curated

Length:814
Mass (Da):89,085
GO
Isoform IBy similarity (identifier: Q9W4T4-1) [UniParc]FASTAAdd to Basket

Also known as: B1 Publication

, S

The sequence of this isoform can be found in the external entry Q9W4T4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,209
Mass (Da):129,413
GO
Isoform IIBy similarity (identifier: P12252-1) [UniParc]FASTAAdd to Basket

Also known as: I1 Publication

, J1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,070
Mass (Da):115,079
GO
Isoform III (identifier: P12252-7) [UniParc]FASTAAdd to Basket

Also known as: E, P

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-429 of isoform II.

Length:642
Mass (Da):70,625
GO
Isoform IVBy similarity (identifier: P12252-3) [UniParc]FASTAAdd to Basket

Also known as: A1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:701
Mass (Da):77,193
GO
Isoform VBy similarity (identifier: P12252-4) [UniParc]FASTAAdd to Basket

Also known as: C1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,057
Mass (Da):113,506
GO
Isoform VIBy similarity (identifier: P12252-5) [UniParc]FASTAAdd to Basket

Also known as: D1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,068
Mass (Da):114,836
GO
Isoform VIIBy similarity (identifier: P12252-6) [UniParc]FASTAAdd to Basket

Also known as: L1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:521
Mass (Da):57,358
GO
Isoform N1 Publication (identifier: Q9W4S9-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.Curated

Length:983
Mass (Da):107,341
GO
Isoform R (identifier: P12252-8) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: Q

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:703
Mass (Da):77,368
GO
Isoform U (identifier: P12252-9) [UniParc]FASTAAdd to Basket

Also known as: T

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:715
Mass (Da):78,887
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAN09606.1.
RefSeqiNP_525061.2. NM_080322.3. [Q8IRU4-1]
UniGeneiDm.4817.

Genome annotation databases

EnsemblMetazoaiFBtr0070521; FBpp0070497; FBgn0000479. [Q8IRU4-1]
GeneIDi31309.
KEGGidme:Dmel_CG32498.
UCSCiCG32498-RA. d. melanogaster. [Q8IRU4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAN09606.1 .
RefSeqi NP_525061.2. NM_080322.3. [Q8IRU4-1 ]
UniGenei Dm.4817.

3D structure databases

ProteinModelPortali Q8IRU4.
SMRi Q8IRU4. Positions 144-178, 253-575.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 57834. 7 interactions.

Proteomic databases

PRIDEi Q8IRU4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0070521 ; FBpp0070497 ; FBgn0000479 . [Q8IRU4-1 ]
GeneIDi 31309.
KEGGi dme:Dmel_CG32498.
UCSCi CG32498-RA. d. melanogaster. [Q8IRU4-1 ]

Organism-specific databases

CTDi 31309.
FlyBasei FBgn0000479. dnc.

Phylogenomic databases

GeneTreei ENSGT00760000118889.
KOi K13293.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .
Reactomei REACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.

Miscellaneous databases

ChiTaRSi dnc. drosophila.
GenomeRNAii 31309.
NextBioi 772953.

Gene expression databases

Bgeei Q8IRU4.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.

Entry informationi

Entry nameiPDE4E_DROME
AccessioniPrimary (citable) accession number: Q8IRU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3