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Protein

NAD-dependent protein deacylase Sirt4

Gene

Sirt4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

NAD-dependent protein deacylase. Catalyzes the NAD-dependent hydrolysis of acyl groups from lysine residues.UniRule annotation

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorUniRule annotation
Metal bindingi160 – 1601ZincUniRule annotation
Metal bindingi163 – 1631ZincUniRule annotation
Metal bindingi211 – 2111ZincUniRule annotation
Metal bindingi214 – 2141ZincUniRule annotation
Binding sitei295 – 2951NAD; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 7321NADUniRule annotationAdd
BLAST
Nucleotide bindingi134 – 1374NADUniRule annotation
Nucleotide bindingi251 – 2533NADUniRule annotation
Nucleotide bindingi277 – 2793NADUniRule annotation

GO - Molecular functioni

  • histone deacetylase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro

GO - Biological processi

  • histone deacetylation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylase Sirt4UniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homolog 4UniRule annotation
Gene namesi
Name:Sirt4
ORF Names:CG3187
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0029783. Sirt4.

Subcellular locationi

  • Mitochondrion matrix UniRule annotation

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616MitochondrionUniRule annotationAdd
BLAST
Chaini17 – 312296NAD-dependent protein deacylase Sirt4PRO_0000417347Add
BLAST

Proteomic databases

PaxDbiQ8IRR5.

Expressioni

Gene expression databases

BgeeiQ8IRR5.
GenevisibleiQ8IRR5. DM.

Interactioni

Protein-protein interaction databases

BioGridi57986. 29 interactions.
IntActiQ8IRR5. 3 interactions.
MINTiMINT-291849.
STRINGi7227.FBpp0070817.

Structurei

3D structure databases

ProteinModelPortaliQ8IRR5.
SMRiQ8IRR5. Positions 31-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 311276Deacetylase sirtuin-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the sirtuin family. Class II subfamily.UniRule annotation
Contains 1 deacetylase sirtuin-type domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2683. Eukaryota.
COG0846. LUCA.
GeneTreeiENSGT00740000115330.
InParanoidiQ8IRR5.
KOiK11414.
OMAiEYVPHHK.
OrthoDBiEOG7DZ8KF.
PhylomeDBiQ8IRR5.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01967. Sirtuin_ClassII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026587. Sirtuin_class_II.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform c (identifier: Q8IRR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVGQLLRFR STSLRSSTAR QEYVPHHKPV VEDDIKRLED FLLSKPNVLV
60 70 80 90 100
LTGAGISTES GIPDYRSEGV GLYARSNHKP VQHMEFVKSS AVRKRYWARN
110 120 130 140 150
FVGWPKFSAT QPNATHHALA RFEREERVQA VVTQNVDRLH TKAGSRNVVE
160 170 180 190 200
VHGSGYVVKC LSCEYRIDRH EFQSILASLN PAFKDAPDMI RPDGDVEIPL
210 220 230 240 250
EYIENFRIPE CTQCGGDLKP EIVFFGDSVP RPRVDQIAGM VYNSDGLLVL
260 270 280 290 300
GSSLLVFSGY RVVLQTKDLK LPVGIVNIGE TRADHLADIK ISAKCGDVIP
310
KLFDFRNSKS VS
Length:312
Mass (Da):34,991
Last modified:June 1, 2003 - v2
Checksum:iA05187624AF8E1B2
GO
Isoform a (identifier: Q8IRR5-2) [UniParc]FASTAAdd to basket

Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:229
Mass (Da):25,666
Checksum:iE263A15F3A6ECC1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741G → A in ACY07062 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8383Missing in isoform a. CuratedVSP_043535Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF46055.3.
AE014298 Genomic DNA. Translation: AAN09147.2.
AE014298 Genomic DNA. Translation: AAN09146.2.
BT011040 mRNA. Translation: AAR30200.1.
BT099555 mRNA. Translation: ACU32639.1.
BT100157 mRNA. Translation: ACY07062.1.
RefSeqiNP_572241.2. NM_132013.3. [Q8IRR5-1]
NP_727013.2. NM_167041.3. [Q8IRR5-2]
NP_727014.2. NM_167042.3. [Q8IRR5-2]
UniGeneiDm.11339.

Genome annotation databases

EnsemblMetazoaiFBtr0070852; FBpp0070817; FBgn0029783. [Q8IRR5-1]
GeneIDi31480.
KEGGidme:Dmel_CG3187.
UCSCiCG3187-RA. d. melanogaster.
CG3187-RC. d. melanogaster. [Q8IRR5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF46055.3.
AE014298 Genomic DNA. Translation: AAN09147.2.
AE014298 Genomic DNA. Translation: AAN09146.2.
BT011040 mRNA. Translation: AAR30200.1.
BT099555 mRNA. Translation: ACU32639.1.
BT100157 mRNA. Translation: ACY07062.1.
RefSeqiNP_572241.2. NM_132013.3. [Q8IRR5-1]
NP_727013.2. NM_167041.3. [Q8IRR5-2]
NP_727014.2. NM_167042.3. [Q8IRR5-2]
UniGeneiDm.11339.

3D structure databases

ProteinModelPortaliQ8IRR5.
SMRiQ8IRR5. Positions 31-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57986. 29 interactions.
IntActiQ8IRR5. 3 interactions.
MINTiMINT-291849.
STRINGi7227.FBpp0070817.

Proteomic databases

PaxDbiQ8IRR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070852; FBpp0070817; FBgn0029783. [Q8IRR5-1]
GeneIDi31480.
KEGGidme:Dmel_CG3187.
UCSCiCG3187-RA. d. melanogaster.
CG3187-RC. d. melanogaster. [Q8IRR5-1]

Organism-specific databases

CTDi23409.
FlyBaseiFBgn0029783. Sirt4.

Phylogenomic databases

eggNOGiKOG2683. Eukaryota.
COG0846. LUCA.
GeneTreeiENSGT00740000115330.
InParanoidiQ8IRR5.
KOiK11414.
OMAiEYVPHHK.
OrthoDBiEOG7DZ8KF.
PhylomeDBiQ8IRR5.

Miscellaneous databases

ChiTaRSiSirt4. fly.
GenomeRNAii31480.
NextBioi773850.
PROiQ8IRR5.

Gene expression databases

BgeeiQ8IRR5.
GenevisibleiQ8IRR5. DM.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01967. Sirtuin_ClassII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026587. Sirtuin_class_II.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  4. Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.E.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.

Entry informationi

Entry nameiSIR4_DROME
AccessioniPrimary (citable) accession number: Q8IRR5
Secondary accession number(s): D0IQB9, Q9W4A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.