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Protein

Glutathione synthetase

Gene

GSS

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (Gclc)
  2. Glutathione synthetase, Glutathione synthetase, Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei132 – 1321SubstrateUniRule annotation
Metal bindingi149 – 1491MagnesiumUniRule annotation
Binding sitei149 – 1491ATPUniRule annotation
Metal bindingi151 – 1511MagnesiumUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Binding sitei313 – 3131ATPUniRule annotation
Metal bindingi375 – 3751MagnesiumUniRule annotation
Binding sitei382 – 3821ATPUniRule annotation
Binding sitei433 – 4331ATPUniRule annotation
Binding sitei458 – 4581SubstrateUniRule annotation
Binding sitei460 – 4601ATPUniRule annotation
Binding sitei466 – 4661ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi371 – 38010ATPUniRule annotation
Nucleotide bindingi404 – 4074ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • detoxification of arsenic-containing substance Source: FlyBase
  • response to ethanol Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:GSSImported
Synonyms:CG32495-RBImported, GS
ORF Names:CG32495Imported, CG6835Imported, Dmel_CG32495Imported, Dmel_CG6835Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0052495. CG32495.
FBgn0030882. GSS.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0290197.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 310101GSH_synthaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
KOiK01920.
OrthoDBiEOG091G05V8.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IQZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDANTAVL RNCIRLPLAE DELLEVTAKA KDYAIMHGAA MRSKTAFSPD
60 70 80 90 100
SLNFAPFVLV PSSFPRKEFE KAVALQPIIN RLMHNVAHDE EFITTTLAET
110 120 130 140 150
IKVDEFTANL FNIYRKVLAH GFTQAYSIGL LRSDYMAHVS EGCAIKQVEI
160 170 180 190 200
NTVASSFAGI ATQLVPQQKF VLSELGHADK LHNMPDNNAL AGLCDGMVKA
210 220 230 240 250
WDIYAKPQAV ILFIIEDVSY NICDQRFHEF YIRETYPHIK VLRRTLTEVH
260 270 280 290 300
REGKLGQSKE LLLGSQEVAV IYFRAGYEPG HYHSQADWDA RYLMETSLAI
310 320 330 340 350
KCPSIHYHLA GTKKVQQALA QPAVLERFIN DPEEIKAVGK IFTGLYSLDD
360 370 380 390 400
NEAGNASYEM ALRTPERFVL KPQREGGGNN VYGVDIPDAL KRMSRVERSA
410 420 430 440 450
WILMDLIHPP LTKGYMVRPG GDMPPQIVDM VSELGIFGVV IGDAEHIVHN
460 470 480 490
YQAGHMLRTK LSTANEGGVA AGLGALDSPY LIDSDDEDEQ K
Length:491
Mass (Da):54,637
Last modified:November 30, 2010 - v2
Checksum:iFF278BA90F1E17E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09448.1.
AE014298 Genomic DNA. Translation: AAN09449.2.
EF419258 mRNA. Translation: ABN58453.1.
EF419259 mRNA. Translation: ABN58454.1.
EF419260 mRNA. Translation: ABN58455.1.
BT099771 mRNA. Translation: ACV88430.1.
AE014298 Genomic DNA. Translation: ACZ95317.1.
AE014298 Genomic DNA. Translation: ACZ95319.1.
RefSeqiNP_001162782.1. NM_001169311.1.
NP_001162784.1. NM_001169313.1.
NP_728116.1. NM_167589.2.
NP_728117.2. NM_167590.2.
UniGeneiDm.23147.
Dm.7230.

Genome annotation databases

EnsemblMetazoaiFBtr0089800; FBpp0088741; FBgn0052495.
FBtr0300969; FBpp0290191; FBgn0030882.
FBtr0300974; FBpp0290196; FBgn0030882.
FBtr0300978; FBpp0290200; FBgn0052495.
GeneIDi318053.
32775.
KEGGidme:Dmel_CG6835.
UCSCiCG32495-RA. d. melanogaster.
CG32495-RB. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09448.1.
AE014298 Genomic DNA. Translation: AAN09449.2.
EF419258 mRNA. Translation: ABN58453.1.
EF419259 mRNA. Translation: ABN58454.1.
EF419260 mRNA. Translation: ABN58455.1.
BT099771 mRNA. Translation: ACV88430.1.
AE014298 Genomic DNA. Translation: ACZ95317.1.
AE014298 Genomic DNA. Translation: ACZ95319.1.
RefSeqiNP_001162782.1. NM_001169311.1.
NP_001162784.1. NM_001169313.1.
NP_728116.1. NM_167589.2.
NP_728117.2. NM_167590.2.
UniGeneiDm.23147.
Dm.7230.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0290197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089800; FBpp0088741; FBgn0052495.
FBtr0300969; FBpp0290191; FBgn0030882.
FBtr0300974; FBpp0290196; FBgn0030882.
FBtr0300978; FBpp0290200; FBgn0052495.
GeneIDi318053.
32775.
KEGGidme:Dmel_CG6835.
UCSCiCG32495-RA. d. melanogaster.
CG32495-RB. d. melanogaster.

Organism-specific databases

CTDi2937.
FlyBaseiFBgn0052495. CG32495.
FBgn0030882. GSS.

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
KOiK01920.
OrthoDBiEOG091G05V8.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
ReactomeiR-DME-174403. Glutathione synthesis and recycling.

Miscellaneous databases

ChiTaRSiG-salpha60A. fly.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.30.470.20. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ8IQZ1_DROME
AccessioniPrimary (citable) accession number: Q8IQZ1
Secondary accession number(s): Q8IQZ2
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: November 30, 2010
Last modified: September 7, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.