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Protein

Glutathione synthetase

Gene

GSS

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (Gclc)
  2. Glutathione synthetase, Glutathione synthetase (GSS), Glutathione synthetase, Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132SubstrateUniRule annotation1
Metal bindingi149MagnesiumUniRule annotation1
Binding sitei149ATPUniRule annotation1
Metal bindingi151MagnesiumUniRule annotation1
Binding sitei225SubstrateUniRule annotation1
Binding sitei313ATPUniRule annotation1
Metal bindingi375MagnesiumUniRule annotation1
Binding sitei382ATPUniRule annotation1
Binding sitei433ATPUniRule annotation1
Binding sitei458SubstrateUniRule annotation1
Binding sitei460ATPUniRule annotation1
Binding sitei466ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi371 – 380ATPUniRule annotation10
Nucleotide bindingi404 – 407ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • detoxification of arsenic-containing substance Source: FlyBase
  • response to ethanol Source: FlyBase

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:GSSImported
Synonyms:32495Imported, 6835Imported, CG32495-RBImported, CG32497Imported, CG33065Imported, Dmel\CG32495Imported, Dmel\CG6835Imported, GSImported
ORF Names:CG32495Imported, CG6835Imported, Dmel_CG32495Imported, Dmel_CG6835Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0052495. CG32495.
FBgn0030882. GSS.

Expressioni

Gene expression databases

BgeeiFBgn0030882.

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini210 – 310GSH_synthaseInterPro annotationAdd BLAST101

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
KOiK21456.
OrthoDBiEOG091G05V8.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiView protein in InterPro
IPR005615. Glutathione_synthase.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_C_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR037013. GSH-S_sub-bd_sf.
IPR004887. GSH_synth_subst-bd.
IPR016185. PreATP-grasp_dom_sf.
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiView protein in Pfam
PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IQZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDANTAVL RNCIRLPLAE DELLEVTAKA KDYAIMHGAA MRSKTAFSPD
60 70 80 90 100
SLNFAPFVLV PSSFPRKEFE KAVALQPIIN RLMHNVAHDE EFITTTLAET
110 120 130 140 150
IKVDEFTANL FNIYRKVLAH GFTQAYSIGL LRSDYMAHVS EGCAIKQVEI
160 170 180 190 200
NTVASSFAGI ATQLVPQQKF VLSELGHADK LHNMPDNNAL AGLCDGMVKA
210 220 230 240 250
WDIYAKPQAV ILFIIEDVSY NICDQRFHEF YIRETYPHIK VLRRTLTEVH
260 270 280 290 300
REGKLGQSKE LLLGSQEVAV IYFRAGYEPG HYHSQADWDA RYLMETSLAI
310 320 330 340 350
KCPSIHYHLA GTKKVQQALA QPAVLERFIN DPEEIKAVGK IFTGLYSLDD
360 370 380 390 400
NEAGNASYEM ALRTPERFVL KPQREGGGNN VYGVDIPDAL KRMSRVERSA
410 420 430 440 450
WILMDLIHPP LTKGYMVRPG GDMPPQIVDM VSELGIFGVV IGDAEHIVHN
460 470 480 490
YQAGHMLRTK LSTANEGGVA AGLGALDSPY LIDSDDEDEQ K
Length:491
Mass (Da):54,637
Last modified:November 30, 2010 - v2
Checksum:iFF278BA90F1E17E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09448.1.
AE014298 Genomic DNA. Translation: AAN09449.2.
EF419258 mRNA. Translation: ABN58453.1.
EF419259 mRNA. Translation: ABN58454.1.
EF419260 mRNA. Translation: ABN58455.1.
BT099771 mRNA. Translation: ACV88430.1.
AE014298 Genomic DNA. Translation: ACZ95317.1.
AE014298 Genomic DNA. Translation: ACZ95319.1.
RefSeqiNP_001162782.1. NM_001169311.1.
NP_001162784.1. NM_001169313.1.
NP_728116.1. NM_167589.2.
NP_728117.2. NM_167590.2.
UniGeneiDm.23147.
Dm.7230.

Genome annotation databases

EnsemblMetazoaiFBtr0089800; FBpp0088741; FBgn0052495.
FBtr0300969; FBpp0290191; FBgn0030882.
FBtr0300974; FBpp0290196; FBgn0030882.
FBtr0300978; FBpp0290200; FBgn0052495.
GeneIDi318053.
32775.
KEGGidme:Dmel_CG6835.
UCSCiCG32495-RA. d. melanogaster.
CG32495-RB. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiQ8IQZ1_DROME
AccessioniPrimary (citable) accession number: Q8IQZ1
Secondary accession number(s): Q8IQZ2
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: November 30, 2010
Last modified: November 22, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported