ID   GLT25_DROME             Reviewed;         612 AA.
AC   Q8IPK4; A9UNF8;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   24-JAN-2024, entry version 123.
DE   RecName: Full=Glycosyltransferase 25 family member;
DE            EC=2.-.-.-;
DE   Flags: Precursor;
GN   ORFNames=CG31915;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RA   Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA   Celniker S.E.;
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-
CC       ProRule:PRU10138}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABY21735.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014134; AAN10543.1; -; Genomic_DNA.
DR   EMBL; BT031322; ABY21735.1; ALT_INIT; mRNA.
DR   RefSeq; NP_723087.1; NM_164645.3.
DR   AlphaFoldDB; Q8IPK4; -.
DR   SMR; Q8IPK4; -.
DR   STRING; 7227.FBpp0078732; -.
DR   CAZy; GT25; Glycosyltransferase Family 25.
DR   GlyGen; Q8IPK4; 4 sites.
DR   PaxDb; 7227-FBpp0078732; -.
DR   DNASU; 319025; -.
DR   EnsemblMetazoa; FBtr0079099; FBpp0078732; FBgn0051915.
DR   GeneID; 319025; -.
DR   KEGG; dme:Dmel_CG31915; -.
DR   UCSC; CG31915-RA; d. melanogaster.
DR   AGR; FB:FBgn0051915; -.
DR   FlyBase; FBgn0051915; CG31915.
DR   VEuPathDB; VectorBase:FBgn0051915; -.
DR   eggNOG; KOG2540; Eukaryota.
DR   eggNOG; KOG4179; Eukaryota.
DR   GeneTree; ENSGT01030000234558; -.
DR   HOGENOM; CLU_024037_2_0_1; -.
DR   InParanoid; Q8IPK4; -.
DR   OMA; QRVYHYV; -.
DR   OrthoDB; 5490945at2759; -.
DR   PhylomeDB; Q8IPK4; -.
DR   Reactome; R-DME-1650814; Collagen biosynthesis and modifying enzymes.
DR   BioGRID-ORCS; 319025; 0 hits in 3 CRISPR screens.
DR   GenomeRNAi; 319025; -.
DR   PRO; PR:Q8IPK4; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0051915; Expressed in embryonic/larval hemocyte (Drosophila) and 29 other cell types or tissues.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; ISS:FlyBase.
DR   GO; GO:0050211; F:procollagen galactosyltransferase activity; ISS:FlyBase.
DR   GO; GO:1904028; P:positive regulation of collagen fibril organization; ISS:FlyBase.
DR   CDD; cd06532; Glyco_transf_25; 1.
DR   InterPro; IPR002654; Glyco_trans_25.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR10730:SF53; GLYCOSYLTRANSFERASE 25 FAMILY MEMBER; 1.
DR   PANTHER; PTHR10730; PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER; 1.
DR   Pfam; PF01755; Glyco_transf_25; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
DR   Genevisible; Q8IPK4; DM.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW   Reference proteome; Signal; Transferase.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..612
FT                   /note="Glycosyltransferase 25 family member"
FT                   /id="PRO_0000309549"
FT   MOTIF           609..612
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT   CARBOHYD        106
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        227
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        263
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        524
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   612 AA;  71149 MW;  5719BCE07C7698B6 CRC64;
     MNKQVIFGLL LACILVCISG QDEEDYQESP TVLIALLVRN KAHILPMFLS YLEQQDYPKE
     RIAIWLRCDH SNDDSIELLR QWLDNSGDLY HSVSYEFKPE EQSFVNGTSP YEWPASRFKH
     LIALKEEAFQ YGRDIWADYV FFLDADVLLT SKDSLKVLTR LQLPIVAPML ISESLYSNFW
     CGMTEDYYYR RTDEYKEIYH VKKQGSFPVP MVHTAVLVNM NHRAVRNLTF DRNKLVELQK
     SRQQEPLYDG PADDIIVFAI SANSSGIPLH ICNDITFGYI LQPLEPGDTL DHDVQQLVNL
     KSIMVNELGA VPPLLDYYKH LEKKPEKSKL SLDRIFMINL KRRPERREKM ERLFDEIGIE
     AEHFPAVDGK ELSTERLLEM GVRFLPGYED PYHHRAMTMG EIGCFLSHYN IWVMMVRKQL
     KEVLILEDDI RFEPYFRQNA VRILNQARNA AQYDLIYFGR KRLKEESEPA VENADNLVHA
     GYSYWTLGYV ISLQGALKLL AAKPLDKLIP VDEFLPLMFD RHPNKTWTEA FPKRNLVAFS
     ASPLLLYPIY YTGESGYISD TEDSQQISVE TSEEGEARLK SDREQVFDHE QEFKLNPELK
     LGESLSKSHQ EL
//