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Protein

Slowpoke-binding protein

Gene

Slob

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of calcium-activated channel Slo. Increases or decreases the voltage sensitivity of Slo, depending on the absence or presence of 14-3-3-zeta in the complex, respectively.1 Publication

GO - Molecular functioni

  • protein kinase activity Source: FlyBase

GO - Biological processi

  • modulation of chemical synaptic transmission Source: UniProtKB
  • neuromuscular synaptic transmission Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of membrane potential Source: FlyBase
  • response to starvation Source: FlyBase

Names & Taxonomyi

Protein namesi
Recommended name:
Slowpoke-binding protein
Gene namesi
Name:Slob
ORF Names:CG43756
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0264087. Slob.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54S → A: Abolishes phosphorylation and interaction with 14-3-3-zeta; when associated with A-79. 1 Publication1
Mutagenesisi79S → A: Abolishes phosphorylation and interaction with 14-3-3-zeta; when associated with A-54. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000719591 – 515Slowpoke-binding proteinAdd BLAST515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Phosphoserine1 Publication1
Modified residuei79Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation of Ser-54 and Ser-79 is required for the interaction with 14-3-3-zeta but not with that of Slo.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8IPH9.
PRIDEiQ8IPH9.

PTM databases

iPTMnetiQ8IPH9.

Expressioni

Tissue specificityi

Expressed in head. In larval brain, it is expressed in the mushroom body. Also expressed in larval muscles.1 Publication

Inductioni

Expressed with a circadian rhythm showing a peak at ZT15 (zeitgeber 15).1 Publication

Gene expression databases

BgeeiFBgn0264087.
ExpressionAtlasiQ8IPH9. differential.
GenevisibleiQ8IPH9. DM.

Interactioni

Subunit structurei

Interacts specifically with Slo; which activates Slo activity. Interacts with 14-3-3-zeta when phosphorylated. Forms a heterotetrameric complex containing phosphorylated Slob, Slo and 14-3-3-zeta, which represses Slo activity due to the indirect interaction between Slo and 14-3-3-zeta.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi60180. 4 interactors.
ELMiQ8IPH9.
IntActiQ8IPH9. 4 interactors.
MINTiMINT-1603713.
STRINGi7227.FBpp0303549.

Structurei

3D structure databases

ProteinModelPortaliQ8IPH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 203Interaction with SloAdd BLAST13

Phylogenomic databases

eggNOGiENOG410IKGX. Eukaryota.
ENOG410YSBT. LUCA.
GeneTreeiENSGT00390000017669.
InParanoidiQ8IPH9.
KOiK17543.
OrthoDBiEOG091G0II9.
PhylomeDBiQ8IPH9.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IPH9-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKFNKAAQQ QRIDNRNSAV TGHDPFVRPP VPEKKVRNIM KKLHKANGLK
60 70 80 90 100
RSNSAIEFDV SALTAANRRQ IYSSNRSASS EQDNSDLSEH SEKSPLVSAR
110 120 130 140 150
LDNLARLFFS KSMPAETGSR DTIDSVLTTR PNIKYQYSAL DSGNGIVERS
160 170 180 190 200
PRERAQREKA LNATQEWIQG ANGRYEVIAH LDEIGSRHGK NWFLVTDASV
210 220 230 240 250
RTDRLQTLLP LPPDCVAFED LPPNECAREI LMELLGSLHH PYIYPVLDLG
260 270 280 290 300
FLRNSSYNYA CLVTPFNSRG SLKDLIYKAQ WNEPWARKYT RKPNGLPVSQ
310 320 330 340 350
VQRLGRQILE ALLFLKERGF PLHGHLHSGN VILQNGAARL SGLENGLLGL
360 370 380 390 400
SSRINAVMWS RSVTEIENVD IVCFGHLLYE MCTGQELTTP KPSMRVLEME
410 420 430 440 450
LQHYPQIGQT RKQILEILGL IFEPPSGVCP SVEDLVLCDL FRSIDLRELR
460 470 480 490 500
GPCFSTIKPS LSRSTLNLLQ AVKKRQCASL GHSLSEANSP CTPPSTPHDR
510
RTGVLPAPYE VFRMY
Length:515
Mass (Da):57,924
Last modified:April 26, 2004 - v2
Checksum:i1B4D86959CCAFAC1
GO
Isoform 2 (identifier: Q8IPH9-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     410-413: Missing.

Show »
Length:511
Mass (Da):57,411
Checksum:iFC9E6BE61A8B6A9B
GO

Sequence cautioni

The sequence AAL28269 differs from that shown. Reason: Frameshift at position 262.Curated
The sequence AAN10635 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13RI → PS in AAC06289 (PubMed:9539129).Curated2
Sequence conflicti47N → T in AAC06289 (PubMed:9539129).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010097410 – 413Missing in isoform 2. 3 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051162 mRNA. Translation: AAC06289.1.
AE014134 Genomic DNA. Translation: AAF52534.1.
AE014134 Genomic DNA. Translation: AAN10635.1. Sequence problems.
AE014134 Genomic DNA. Translation: ABC65880.1.
BT021228 mRNA. Translation: AAX33376.1.
AY060721 mRNA. Translation: AAL28269.1. Frameshift.
RefSeqiNP_001033881.1. NM_001038792.1. [Q8IPH9-2]
NP_001188710.1. NM_001201781.1. [Q8IPH9-2]
NP_477349.1. NM_058001.3. [Q8IPH9-1]
NP_723292.1. NM_164756.3.
NP_723293.1. NM_164757.2. [Q8IPH9-1]
UniGeneiDm.5645.

Genome annotation databases

EnsemblMetazoaiFBtr0330697; FBpp0303540; FBgn0264087. [Q8IPH9-1]
FBtr0330698; FBpp0303541; FBgn0264087.
FBtr0330699; FBpp0303542; FBgn0264087. [Q8IPH9-1]
FBtr0330700; FBpp0303543; FBgn0264087. [Q8IPH9-2]
FBtr0330701; FBpp0303544; FBgn0264087. [Q8IPH9-2]
GeneIDi34038.
KEGGidme:Dmel_CG43756.
UCSCiCG6772-RD. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSLOB_DROME
AccessioniPrimary (citable) accession number: Q8IPH9
Secondary accession number(s): O61564
, Q5BIJ6, Q95SL3, Q9VLZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: September 27, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase