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Q8INK6

- PGPLB_DROME

UniProt

Q8INK6 - PGPLB_DROME

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Protein

Peptidoglycan-recognition protein LB

Gene

PGRP-LB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Zinc
Metal bindingi169 – 1691Zinc
Metal bindingi177 – 1771Zinc

GO - Molecular functioni

  1. amidase activity Source: FlyBase
  2. N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
  3. peptidoglycan binding Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. defense response to Gram-positive bacterium Source: UniProtKB
  2. immune response Source: FlyBase
  3. innate immune response Source: UniProtKB
  4. negative regulation of peptidoglycan recognition protein signaling pathway Source: FlyBase
  5. peptidoglycan catabolic process Source: UniProtKB
  6. peptidoglycan metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein LB (EC:3.5.1.28)
Gene namesi
Name:PGRP-LB
ORF Names:CG14704
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037906. PGRP-LB.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi175 – 1751T → K: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence AnalysisAdd
BLAST
Chaini16 – 232217Peptidoglycan-recognition protein LBPRO_0000023905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 731 Publication
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8INK6.
PRIDEiQ8INK6.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed from old embryos. Expressed in larvae and adults.1 Publication

Inductioni

Strongly up-regulated by PGN from B.subtilis. Regulated by the imd/Relish pathway.2 Publications

Gene expression databases

BgeeiQ8INK6.
ExpressionAtlasiQ8INK6. differential.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CG16790Q9VHB31EBI-117332,EBI-92020

Protein-protein interaction databases

BioGridi66511. 2 interactions.
IntActiQ8INK6. 1 interaction.
MINTiMINT-1594493.

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 383
Beta strandi51 – 6010
Beta strandi62 – 643
Helixi70 – 8617
Beta strandi94 – 996
Beta strandi105 – 1095
Beta strandi116 – 1183
Turni119 – 1235
Beta strandi124 – 1329
Beta strandi135 – 1373
Helixi141 – 15616
Beta strandi159 – 16810
Helixi169 – 1713
Beta strandi173 – 1753
Helixi180 – 1867
Helixi195 – 1973

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OHTX-ray2.00A18-232[»]
ProteinModelPortaliQ8INK6.
SMRiQ8INK6. Positions 29-201.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8INK6.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248951.
GeneTreeiENSGT00390000016833.
InParanoidiQ8INK6.
KOiK01446.
OrthoDBiEOG757CZ5.
PhylomeDBiQ8INK6.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform C (identifier: Q8INK6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTALGLVLLS MMGYSQHMQQ ANLGDGVATA RLLSRSDWGA RLPKSVEHFQ
60 70 80 90 100
GPAPYVIIHH SYMPAVCYST PDCMKSMRDM QDFHQLERGW NDIGYSFGIG
110 120 130 140 150
GDGMIYTGRG FNVIGAHAPK YNDKSVGIVL IGDWRTELPP KQMLDAAKNL
160 170 180 190 200
IAFGVFKGYI DPAYKLLGHR QVRDTECPGG RLFAEISSWP HFTHINDTEG
210 220 230
VSSTTAPVVP HVHPQAAAPQ KPHQSPPAAP KV

Note: No experimental confirmation available.

Length:232
Mass (Da):25,436
Last modified:March 1, 2003 - v1
Checksum:i7BD18D4EC41F021E
GO
Isoform A (identifier: Q8INK6-2) [UniParc]FASTAAdd to Basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Does not contain a signal sequence.

Show »
Length:215
Mass (Da):23,602
Checksum:iAC661CAEBBE63215
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717Missing in isoform A. 2 PublicationsVSP_013593Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF207537 mRNA. Translation: AAG23731.1.
AF207538 mRNA. Translation: AAG23732.1.
AE014297 Genomic DNA. Translation: AAF54643.1.
AE014297 Genomic DNA. Translation: AAN13505.1.
AY060759 mRNA. Translation: AAL28307.1.
BT011455 mRNA. Translation: AAR99113.1.
RefSeqiNP_001247053.1. NM_001260124.2. [Q8INK6-2]
NP_001247054.1. NM_001260125.2. [Q8INK6-2]
NP_650079.1. NM_141822.3. [Q8INK6-2]
NP_731575.1. NM_169392.2. [Q8INK6-1]
NP_731576.1. NM_169393.3. [Q8INK6-2]
UniGeneiDm.3374.

Genome annotation databases

EnsemblMetazoaiFBtr0082396; FBpp0081872; FBgn0037906. [Q8INK6-1]
GeneIDi41379.
KEGGidme:Dmel_CG14704.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF207537 mRNA. Translation: AAG23731.1 .
AF207538 mRNA. Translation: AAG23732.1 .
AE014297 Genomic DNA. Translation: AAF54643.1 .
AE014297 Genomic DNA. Translation: AAN13505.1 .
AY060759 mRNA. Translation: AAL28307.1 .
BT011455 mRNA. Translation: AAR99113.1 .
RefSeqi NP_001247053.1. NM_001260124.2. [Q8INK6-2 ]
NP_001247054.1. NM_001260125.2. [Q8INK6-2 ]
NP_650079.1. NM_141822.3. [Q8INK6-2 ]
NP_731575.1. NM_169392.2. [Q8INK6-1 ]
NP_731576.1. NM_169393.3. [Q8INK6-2 ]
UniGenei Dm.3374.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OHT X-ray 2.00 A 18-232 [» ]
ProteinModelPortali Q8INK6.
SMRi Q8INK6. Positions 29-201.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66511. 2 interactions.
IntActi Q8INK6. 1 interaction.
MINTi MINT-1594493.

Proteomic databases

PaxDbi Q8INK6.
PRIDEi Q8INK6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0082396 ; FBpp0081872 ; FBgn0037906 . [Q8INK6-1 ]
GeneIDi 41379.
KEGGi dme:Dmel_CG14704.

Organism-specific databases

CTDi 41379.
FlyBasei FBgn0037906. PGRP-LB.

Phylogenomic databases

eggNOGi NOG248951.
GeneTreei ENSGT00390000016833.
InParanoidi Q8INK6.
KOi K01446.
OrthoDBi EOG757CZ5.
PhylomeDBi Q8INK6.

Miscellaneous databases

EvolutionaryTracei Q8INK6.
GenomeRNAii 41379.
NextBioi 823566.

Gene expression databases

Bgeei Q8INK6.
ExpressionAtlasi Q8INK6. differential.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view ]
PANTHERi PTHR11022. PTHR11022. 1 hit.
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037945. PGRPs. 1 hit.
SMARTi SM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
    Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
    Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "The Toll and Imd pathways are the major regulators of the immune response in Drosophila."
    De Gregorio E., Spellman P.T., Tzou P., Rubin G.M., Lemaitre B.
    EMBO J. 21:2568-2579(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Crystal structure of peptidoglycan recognition protein LB from Drosophila melanogaster."
    Kim M.-S., Byun M., Oh B.-H.
    Nat. Immunol. 4:787-793(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-215 (ISOFORM A) IN COMPLEX WITH ZINC, FUNCTION, SUBUNIT, DISULFIDE BOND, MUTAGENESIS OF THR-175.

Entry informationi

Entry nameiPGPLB_DROME
AccessioniPrimary (citable) accession number: Q8INK6
Secondary accession number(s): Q9VGN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3