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Q8INK6

- PGPLB_DROME

UniProt

Q8INK6 - PGPLB_DROME

Protein

Peptidoglycan-recognition protein LB

Gene

PGRP-LB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity.1 Publication

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Zinc
    Metal bindingi169 – 1691Zinc
    Metal bindingi177 – 1771Zinc

    GO - Molecular functioni

    1. amidase activity Source: FlyBase
    2. N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
    3. peptidoglycan binding Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. defense response to Gram-positive bacterium Source: UniProtKB
    2. immune response Source: FlyBase
    3. innate immune response Source: UniProtKB
    4. negative regulation of peptidoglycan recognition protein signaling pathway Source: FlyBase
    5. peptidoglycan catabolic process Source: UniProtKB
    6. peptidoglycan metabolic process Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Immunity, Innate immunity

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidoglycan-recognition protein LB (EC:3.5.1.28)
    Gene namesi
    Name:PGRP-LB
    ORF Names:CG14704
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0037906. PGRP-LB.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi175 – 1751T → K: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Chaini16 – 232217Peptidoglycan-recognition protein LBPRO_0000023905Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 731 Publication
    Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8INK6.
    PRIDEiQ8INK6.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Developmental stagei

    Expressed from old embryos. Expressed in larvae and adults.1 Publication

    Inductioni

    Strongly up-regulated by PGN from B.subtilis. Regulated by the imd/Relish pathway.2 Publications

    Gene expression databases

    BgeeiQ8INK6.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CG16790Q9VHB31EBI-117332,EBI-92020

    Protein-protein interaction databases

    BioGridi66511. 2 interactions.
    IntActiQ8INK6. 1 interaction.
    MINTiMINT-1594493.

    Structurei

    Secondary structure

    1
    232
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi36 – 383
    Beta strandi51 – 6010
    Beta strandi62 – 643
    Helixi70 – 8617
    Beta strandi94 – 996
    Beta strandi105 – 1095
    Beta strandi116 – 1183
    Turni119 – 1235
    Beta strandi124 – 1329
    Beta strandi135 – 1373
    Helixi141 – 15616
    Beta strandi159 – 16810
    Helixi169 – 1713
    Beta strandi173 – 1753
    Helixi180 – 1867
    Helixi195 – 1973

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OHTX-ray2.00A18-232[»]
    ProteinModelPortaliQ8INK6.
    SMRiQ8INK6. Positions 29-201.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8INK6.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248951.
    GeneTreeiENSGT00390000016833.
    InParanoidiQ8INK6.
    KOiK01446.
    OrthoDBiEOG757CZ5.
    PhylomeDBiQ8INK6.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR017331. Peptidoglycan_recognition.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view]
    PANTHERiPTHR11022. PTHR11022. 1 hit.
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037945. PGRPs. 1 hit.
    SMARTiSM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform C (identifier: Q8INK6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTALGLVLLS MMGYSQHMQQ ANLGDGVATA RLLSRSDWGA RLPKSVEHFQ    50
    GPAPYVIIHH SYMPAVCYST PDCMKSMRDM QDFHQLERGW NDIGYSFGIG 100
    GDGMIYTGRG FNVIGAHAPK YNDKSVGIVL IGDWRTELPP KQMLDAAKNL 150
    IAFGVFKGYI DPAYKLLGHR QVRDTECPGG RLFAEISSWP HFTHINDTEG 200
    VSSTTAPVVP HVHPQAAAPQ KPHQSPPAAP KV 232

    Note: No experimental confirmation available.

    Length:232
    Mass (Da):25,436
    Last modified:March 1, 2003 - v1
    Checksum:i7BD18D4EC41F021E
    GO
    Isoform A (identifier: Q8INK6-2) [UniParc]FASTAAdd to Basket

    Also known as: B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-17: Missing.

    Note: Does not contain a signal sequence.

    Show »
    Length:215
    Mass (Da):23,602
    Checksum:iAC661CAEBBE63215
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1717Missing in isoform A. 2 PublicationsVSP_013593Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207537 mRNA. Translation: AAG23731.1.
    AF207538 mRNA. Translation: AAG23732.1.
    AE014297 Genomic DNA. Translation: AAF54643.1.
    AE014297 Genomic DNA. Translation: AAN13505.1.
    AY060759 mRNA. Translation: AAL28307.1.
    BT011455 mRNA. Translation: AAR99113.1.
    RefSeqiNP_001247053.1. NM_001260124.1. [Q8INK6-2]
    NP_001247054.1. NM_001260125.1. [Q8INK6-2]
    NP_650079.1. NM_141822.3. [Q8INK6-2]
    NP_731575.1. NM_169392.2. [Q8INK6-1]
    NP_731576.1. NM_169393.2. [Q8INK6-2]
    UniGeneiDm.3374.

    Genome annotation databases

    EnsemblMetazoaiFBtr0082396; FBpp0081872; FBgn0037906. [Q8INK6-1]
    GeneIDi41379.
    KEGGidme:Dmel_CG14704.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207537 mRNA. Translation: AAG23731.1 .
    AF207538 mRNA. Translation: AAG23732.1 .
    AE014297 Genomic DNA. Translation: AAF54643.1 .
    AE014297 Genomic DNA. Translation: AAN13505.1 .
    AY060759 mRNA. Translation: AAL28307.1 .
    BT011455 mRNA. Translation: AAR99113.1 .
    RefSeqi NP_001247053.1. NM_001260124.1. [Q8INK6-2 ]
    NP_001247054.1. NM_001260125.1. [Q8INK6-2 ]
    NP_650079.1. NM_141822.3. [Q8INK6-2 ]
    NP_731575.1. NM_169392.2. [Q8INK6-1 ]
    NP_731576.1. NM_169393.2. [Q8INK6-2 ]
    UniGenei Dm.3374.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OHT X-ray 2.00 A 18-232 [» ]
    ProteinModelPortali Q8INK6.
    SMRi Q8INK6. Positions 29-201.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66511. 2 interactions.
    IntActi Q8INK6. 1 interaction.
    MINTi MINT-1594493.

    Proteomic databases

    PaxDbi Q8INK6.
    PRIDEi Q8INK6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0082396 ; FBpp0081872 ; FBgn0037906 . [Q8INK6-1 ]
    GeneIDi 41379.
    KEGGi dme:Dmel_CG14704.

    Organism-specific databases

    CTDi 41379.
    FlyBasei FBgn0037906. PGRP-LB.

    Phylogenomic databases

    eggNOGi NOG248951.
    GeneTreei ENSGT00390000016833.
    InParanoidi Q8INK6.
    KOi K01446.
    OrthoDBi EOG757CZ5.
    PhylomeDBi Q8INK6.

    Miscellaneous databases

    EvolutionaryTracei Q8INK6.
    GenomeRNAii 41379.
    NextBioi 823566.

    Gene expression databases

    Bgeei Q8INK6.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR017331. Peptidoglycan_recognition.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view ]
    PANTHERi PTHR11022. PTHR11022. 1 hit.
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037945. PGRPs. 1 hit.
    SMARTi SM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
      Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
      Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Embryo.
    5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
      Strain: Berkeley.
      Tissue: Embryo.
    6. "The Toll and Imd pathways are the major regulators of the immune response in Drosophila."
      De Gregorio E., Spellman P.T., Tzou P., Rubin G.M., Lemaitre B.
      EMBO J. 21:2568-2579(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Crystal structure of peptidoglycan recognition protein LB from Drosophila melanogaster."
      Kim M.-S., Byun M., Oh B.-H.
      Nat. Immunol. 4:787-793(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-215 (ISOFORM A) IN COMPLEX WITH ZINC, FUNCTION, SUBUNIT, DISULFIDE BOND, MUTAGENESIS OF THR-175.

    Entry informationi

    Entry nameiPGPLB_DROME
    AccessioniPrimary (citable) accession number: Q8INK6
    Secondary accession number(s): Q9VGN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3