Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptidoglycan-recognition protein LB

Gene

PGRP-LB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Zinc1
Metal bindingi169Zinc1
Metal bindingi177Zinc1

GO - Molecular functioni

  • amidase activity Source: FlyBase
  • N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
  • peptidoglycan binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • defense response to Gram-positive bacterium Source: UniProtKB
  • immune response Source: FlyBase
  • innate immune response Source: UniProtKB
  • negative regulation of peptidoglycan recognition protein signaling pathway Source: FlyBase
  • peptidoglycan catabolic process Source: UniProtKB
  • peptidoglycan metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein LB (EC:3.5.1.28)
Gene namesi
Name:PGRP-LB
ORF Names:CG14704
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037906. PGRP-LB.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi175T → K: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_000002390516 – 232Peptidoglycan-recognition protein LBAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 731 Publication
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8INK6.
PRIDEiQ8INK6.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed from old embryos. Expressed in larvae and adults.1 Publication

Inductioni

Strongly up-regulated by PGN from B.subtilis. Regulated by the imd/Relish pathway.2 Publications

Gene expression databases

BgeeiFBgn0037906.
ExpressionAtlasiQ8INK6. baseline.
GenevisibleiQ8INK6. DM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi66511. 2 interactors.
IntActiQ8INK6. 1 interactor.
MINTiMINT-1594493.
STRINGi7227.FBpp0297234.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 38Combined sources3
Beta strandi51 – 60Combined sources10
Beta strandi62 – 64Combined sources3
Helixi70 – 86Combined sources17
Beta strandi94 – 99Combined sources6
Beta strandi105 – 109Combined sources5
Beta strandi116 – 118Combined sources3
Turni119 – 123Combined sources5
Beta strandi124 – 132Combined sources9
Beta strandi135 – 137Combined sources3
Helixi141 – 156Combined sources16
Beta strandi159 – 168Combined sources10
Helixi169 – 171Combined sources3
Beta strandi173 – 175Combined sources3
Helixi180 – 186Combined sources7
Helixi195 – 197Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OHTX-ray2.00A18-232[»]
ProteinModelPortaliQ8INK6.
SMRiQ8INK6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8INK6.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
InParanoidiQ8INK6.
KOiK01446.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ8INK6.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C (identifier: Q8INK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTALGLVLLS MMGYSQHMQQ ANLGDGVATA RLLSRSDWGA RLPKSVEHFQ
60 70 80 90 100
GPAPYVIIHH SYMPAVCYST PDCMKSMRDM QDFHQLERGW NDIGYSFGIG
110 120 130 140 150
GDGMIYTGRG FNVIGAHAPK YNDKSVGIVL IGDWRTELPP KQMLDAAKNL
160 170 180 190 200
IAFGVFKGYI DPAYKLLGHR QVRDTECPGG RLFAEISSWP HFTHINDTEG
210 220 230
VSSTTAPVVP HVHPQAAAPQ KPHQSPPAAP KV
Note: No experimental confirmation available.
Length:232
Mass (Da):25,436
Last modified:March 1, 2003 - v1
Checksum:i7BD18D4EC41F021E
GO
Isoform A (identifier: Q8INK6-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Does not contain a signal sequence.
Show »
Length:215
Mass (Da):23,602
Checksum:iAC661CAEBBE63215
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135931 – 17Missing in isoform A. 2 PublicationsAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207537 mRNA. Translation: AAG23731.1.
AF207538 mRNA. Translation: AAG23732.1.
AE014297 Genomic DNA. Translation: AAF54643.1.
AE014297 Genomic DNA. Translation: AAN13505.1.
AY060759 mRNA. Translation: AAL28307.1.
BT011455 mRNA. Translation: AAR99113.1.
RefSeqiNP_001247053.1. NM_001260124.2. [Q8INK6-2]
NP_001247054.1. NM_001260125.2. [Q8INK6-2]
NP_650079.1. NM_141822.3. [Q8INK6-2]
NP_731575.1. NM_169392.2. [Q8INK6-1]
NP_731576.1. NM_169393.3. [Q8INK6-2]
UniGeneiDm.3374.

Genome annotation databases

EnsemblMetazoaiFBtr0082396; FBpp0081872; FBgn0037906. [Q8INK6-1]
GeneIDi41379.
KEGGidme:Dmel_CG14704.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207537 mRNA. Translation: AAG23731.1.
AF207538 mRNA. Translation: AAG23732.1.
AE014297 Genomic DNA. Translation: AAF54643.1.
AE014297 Genomic DNA. Translation: AAN13505.1.
AY060759 mRNA. Translation: AAL28307.1.
BT011455 mRNA. Translation: AAR99113.1.
RefSeqiNP_001247053.1. NM_001260124.2. [Q8INK6-2]
NP_001247054.1. NM_001260125.2. [Q8INK6-2]
NP_650079.1. NM_141822.3. [Q8INK6-2]
NP_731575.1. NM_169392.2. [Q8INK6-1]
NP_731576.1. NM_169393.3. [Q8INK6-2]
UniGeneiDm.3374.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OHTX-ray2.00A18-232[»]
ProteinModelPortaliQ8INK6.
SMRiQ8INK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66511. 2 interactors.
IntActiQ8INK6. 1 interactor.
MINTiMINT-1594493.
STRINGi7227.FBpp0297234.

Proteomic databases

PaxDbiQ8INK6.
PRIDEiQ8INK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082396; FBpp0081872; FBgn0037906. [Q8INK6-1]
GeneIDi41379.
KEGGidme:Dmel_CG14704.

Organism-specific databases

CTDi41379.
FlyBaseiFBgn0037906. PGRP-LB.

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
InParanoidiQ8INK6.
KOiK01446.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ8INK6.

Enzyme and pathway databases

ReactomeiR-DME-6803157. Antimicrobial peptides.

Miscellaneous databases

EvolutionaryTraceiQ8INK6.
GenomeRNAii41379.
PROiQ8INK6.

Gene expression databases

BgeeiFBgn0037906.
ExpressionAtlasiQ8INK6. baseline.
GenevisibleiQ8INK6. DM.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGPLB_DROME
AccessioniPrimary (citable) accession number: Q8INK6
Secondary accession number(s): Q9VGN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.