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Q8INK6 (PGPLB_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidoglycan-recognition protein LB

EC=3.5.1.28
Gene names
Name:PGRP-LB
ORF Names:CG14704
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity. Ref.7

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactor

Binds 1 zinc ion per subunit.

Subunit structure

Monomer. Ref.7

Subcellular location

Isoform C: Secreted Potential.

Tissue specificity

Widely expressed. Ref.1

Developmental stage

Expressed from old embryos. Expressed in larvae and adults. Ref.1

Induction

Strongly up-regulated by PGN from B.subtilis. Regulated by the imd/Relish pathway. Ref.1 Ref.6

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CG16790Q9VHB31EBI-117332,EBI-92020

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform C (identifier: Q8INK6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform A (identifier: Q8INK6-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.
Note: Does not contain a signal sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Chain16 – 232217Peptidoglycan-recognition protein LB
PRO_0000023905

Sites

Metal binding591Zinc
Metal binding1691Zinc
Metal binding1771Zinc

Amino acid modifications

Glycosylation1961N-linked (GlcNAc...) Potential
Disulfide bond67 ↔ 73 Ref.7

Natural variations

Alternative sequence1 – 1717Missing in isoform A.
VSP_013593

Experimental info

Mutagenesis1751T → K: Loss of function. Ref.7

Secondary structure

.............................. 232
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform C [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7BD18D4EC41F021E

FASTA23225,436
        10         20         30         40         50         60 
MTALGLVLLS MMGYSQHMQQ ANLGDGVATA RLLSRSDWGA RLPKSVEHFQ GPAPYVIIHH 

        70         80         90        100        110        120 
SYMPAVCYST PDCMKSMRDM QDFHQLERGW NDIGYSFGIG GDGMIYTGRG FNVIGAHAPK 

       130        140        150        160        170        180 
YNDKSVGIVL IGDWRTELPP KQMLDAAKNL IAFGVFKGYI DPAYKLLGHR QVRDTECPGG 

       190        200        210        220        230 
RLFAEISSWP HFTHINDTEG VSSTTAPVVP HVHPQAAAPQ KPHQSPPAAP KV 

« Hide

Isoform A (B) [UniParc].

Checksum: AC661CAEBBE63215
Show »

FASTA21523,602

References

« Hide 'large scale' references
[1]"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[5]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
Strain: Berkeley.
Tissue: Embryo.
[6]"The Toll and Imd pathways are the major regulators of the immune response in Drosophila."
De Gregorio E., Spellman P.T., Tzou P., Rubin G.M., Lemaitre B.
EMBO J. 21:2568-2579(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Crystal structure of peptidoglycan recognition protein LB from Drosophila melanogaster."
Kim M.-S., Byun M., Oh B.-H.
Nat. Immunol. 4:787-793(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-215 (ISOFORM A) IN COMPLEX WITH ZINC, FUNCTION, SUBUNIT, DISULFIDE BOND, MUTAGENESIS OF THR-175.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF207537 mRNA. Translation: AAG23731.1.
AF207538 mRNA. Translation: AAG23732.1.
AE014297 Genomic DNA. Translation: AAF54643.1.
AE014297 Genomic DNA. Translation: AAN13505.1.
AY060759 mRNA. Translation: AAL28307.1.
BT011455 mRNA. Translation: AAR99113.1.
RefSeqNP_001247053.1. NM_001260124.1.
NP_001247054.1. NM_001260125.1.
NP_650079.1. NM_141822.3.
NP_731575.1. NM_169392.2.
NP_731576.1. NM_169393.2.
UniGeneDm.3374.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OHTX-ray2.00A18-232[»]
ProteinModelPortalQ8INK6.
SMRQ8INK6. Positions 29-201.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66511. 2 interactions.
IntActQ8INK6. 1 interaction.
MINTMINT-1594493.

Proteomic databases

PaxDbQ8INK6.
PRIDEQ8INK6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0082396; FBpp0081872; FBgn0037906. [Q8INK6-1]
GeneID41379.
KEGGdme:Dmel_CG14704.

Organism-specific databases

CTD41379.
FlyBaseFBgn0037906. PGRP-LB.

Phylogenomic databases

eggNOGNOG248951.
GeneTreeENSGT00390000016833.
InParanoidQ8INK6.
KOK01446.
OrthoDBEOG757CZ5.
PhylomeDBQ8INK6.

Gene expression databases

BgeeQ8INK6.

Family and domain databases

Gene3D3.40.80.10. 1 hit.
InterProIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERPTHR11022. PTHR11022. 1 hit.
PfamPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFPIRSF037945. PGRPs. 1 hit.
SMARTSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMSSF55846. SSF55846. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ8INK6.
GenomeRNAi41379.
NextBio823566.

Entry information

Entry namePGPLB_DROME
AccessionPrimary (citable) accession number: Q8INK6
Secondary accession number(s): Q9VGN3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase