Reviewed, UniProtKB/Swiss-Prot Q8IN81 (FRU_DROME)

Names and origin

Protein names

Recommended name:
    Sex determination protein fruitless

Gene names

Name:	fru
Synonyms:	BTB-VI
ORF Names:	CG14307

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably acts as a transcriptional regulator. Part of the somatic sex determination hierarchy; sex determination genes transformer (tra) and transformer-2 (tra-2) switch fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site. Vital for the development of males and females. Controls the development of the male specific abdominal muscle of Lawrence. Plays a role in male courtship behavior and sexual orientation. Enhances male-specific expression of takeout in brain-associated fat body. Ref.1 Ref.2 Ref.3 Ref.7 Ref.8 Ref.4 Ref.6
Subcellular location	Nucleus Potential. Ref.6
Tissue specificity	Expressed in parts of the adult male brain associated with the courtship song and steps of the male courtship. Also expressed in the larval and pupal male mushroom body and optic lobe. Expressed in pupal female optic lobe. Ref.1 Ref.2 Ref.3 Ref.4
Disruption phenotype	Mutant males exhibit bisexual behavior; they court females but are behaviorally sterile so fail to mate and they exhibit vigorous courtship with other fru mutant males. Ref.1
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aggressive behavior Inferred from mutant phenotype. Source: FlyBase central nervous system development Inferred from mutant phenotype. Source: FlyBase copulation Traceable author statement. Source: FlyBase male courtship behavior, orientation prior to leg tapping and wing vibration Non-traceable author statement. Source: FlyBase male courtship behavior, veined wing generated song production Ref.1 Inferred from mutant phenotype. Source: UniProtKB male sex differentiation Traceable author statement. Source: FlyBase mating behavior, sex discrimination Traceable author statement. Source: FlyBase muscle organ development Ref.1 Ref.3 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW sex determination Ref.1 Ref.3 Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Ref.6 Non-traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG10323	Q9V4B8	1	EBI-196831,EBI-145217

Alternative products

This entry describes 10 isoforms produced by alternative splicing. [Align] [Select] Note: Sex specific splicing is controlled by tra and tra-2. Sex-specific repression of the translation of fru mRNA might be mediated by the binding of Tra to the fru mRNA in females.
Isoform Male-A Ref.4 (identifier: Q8IN81-1) Also known as: E; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Female-A Ref.4 (identifier: Q8IN81-4) Also known as: C; The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.
Isoform Female-B Ref.4 (identifier: Q8IN81-7) Also known as: F; The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.      617-789: RVKCFNIKHD...YHGHANHQLH → SSARHHLSTP...DRLLKLNSSI      790-955: Missing.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	650	1	T → A in AAG28589. Ref.3
Isoform Female-E Ref.4 (identifier: Q8IN81-10) Also known as: H; The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.      617-796: RVKCFNIKHD...QLHQHPPSAT → SKAWHMRLTF...QQQQHNATSE      797-955: Missing.
Isoform Female-I Ref.1 (identifier: Q8IN81-3) The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.      324-336: Missing.      617-789: RVKCFNIKHD...YHGHANHQLH → SSARHHLSTP...DRLLKLNSSI      790-955: Missing.
Isoform Male-B Ref.4 (identifier: Q8IN81-6) Also known as: B; The sequence of this isoform differs from the canonical sequence as follows:      617-789: RVKCFNIKHD...YHGHANHQLH → SSARHHLSTP...DRLLKLNSSI      790-955: Missing.
Isoform Male-E Ref.4 (identifier: Q8IN81-12) Also known as: G; The sequence of this isoform differs from the canonical sequence as follows:      617-796: RVKCFNIKHD...QLHQHPPSAT → SKAWHMRLTF...QQQQHNATSE      797-955: Missing.
Isoform Male-I Ref.1 (identifier: Q8IN81-2) The sequence of this isoform differs from the canonical sequence as follows:      324-336: Missing.      617-789: RVKCFNIKHD...YHGHANHQLH → SSARHHLSTP...DRLLKLNSSI      790-955: Missing.
Isoform Type-C Ref.4 (identifier: Q8IN81-8) Also known as: D; The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.      572-766: SAALKLHAED...PPVANNQAYL → VKKSEAFLGS...ITINVGKNSA      767-955: Missing.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	539	1	S → P in AAG28590. Ref.3
	Sequence conflict	617	1	H → R in AAG28590. Ref.3
Isoform Type-D Ref.4 (identifier: Q8IN81-9) Also known as: A; The sequence of this isoform differs from the canonical sequence as follows:      1-101: Missing.      617-617: R → E      618-955: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 955	955	Sex determination protein fruitless		PRO_0000046921
Regions
	Domain	131 – 196	66	BTB
	Zinc finger	918 – 941	24	C2H2-type
	Compositional bias	34 – 56	23	His-rich
	Compositional bias	77 – 93	17	Pro-rich
	Compositional bias	292 – 357	66	Ala-rich
	Compositional bias	386 – 426	41	Asn-rich
	Compositional bias	799 – 870	72	Ala/Ser-rich
Natural variations
	Alternative sequence	1 – 101	101	Missing in isoform Female-I, isoform Female-A, isoform Type-D, isoform Type-C, isoform Female-B and isoform Female-E. Ref.1 Ref.4		VSP_050497
	Alternative sequence	324 – 336	13	Missing in isoform Female-I and isoform Male-I. Ref.1		VSP_050498
	Alternative sequence	572 – 766	195	SAALK…NQAYL → VKKSEAFLGSTGNKSMHQML LHQAVEAQLKSFQLHYQNEG LDSAMHRLLAQQQQHQEQQQ QHQQQPHHSLGKSQSPAIPS GSAGGSSRKSGRFRANWLYQ FEWLQYDERANTMFCRHCRK WSGELADIRTSFVEGNSNFR LEIVNHHNKCKSHRMCYERE LQEQQQHPMPSGSAGGSSKR RSPEIITINVGKNSA in isoform Type-C. Ref.4		VSP_050499
	Alternative sequence	617 – 796	180	RVKCF…PPSAT → SKAWHMRLTFERLSGGCNLH RCKLCGKVVTHIRNHYHVHF PGRFECPLCRATYTRSDNLR THCKFKHPMYNPDTRKFDNL MSSSAVGAAATPTASQLAVA SQAAMAAAAAAAFNAAQQQQ QQQQQQQQHQHQQQQQHQQS QQQQQQQQQSQQQLHALAQQ HMLQLQPQHHQQQQHNATSE in isoform Female-E and isoform Male-E. Ref.4		VSP_050500
	Alternative sequence	617 – 789	173	RVKCF…NHQLH → SSARHHLSTPLSTSSSASPP PPPFGMHLSAALKREYHPLH YMAAGNGHNGPSALGYGNQG SGNAPNSAGGAGSVAGGVGA GGGAGGATGAAGHNSHHTMS YHNMFTPSRDPGTMWRCRSC GKEVTNRWHHFHSHTAQRSM CPYCPATYSRIDTLRSHLRV KHPDRLLKLNSSI in isoform Female-I, isoform Male-I, isoform Female-B and isoform Male-B. Ref.1 Ref.4		VSP_050501
	Alternative sequence	617	1	R → E in isoform Type-D. Ref.4		VSP_050502
	Alternative sequence	618 – 955	338	Missing in isoform Type-D. Ref.4		VSP_050503
	Alternative sequence	767 – 955	189	Missing in isoform Type-C. Ref.4		VSP_050504
	Alternative sequence	790 – 955	166	Missing in isoform Female-I, isoform Male-I, isoform Female-B and isoform Male-B. Ref.1 Ref.4		VSP_050505
	Alternative sequence	797 – 955	159	Missing in isoform Female-E and isoform Male-E. Ref.4		VSP_050506
Experimental info
	Sequence conflict	107	1	C → S in AAA50838. Ref.6
	Sequence conflict	131	1	C → R in BAA12664. Ref.3
	Sequence conflict	212 – 218	7	RGLTDNN → CANQGSI in AAA50838. Ref.6
	Sequence conflict	722 – 723	2	RA → QRN Ref.2
	Sequence conflict	722 – 723	2	RA → QRN Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Male-A (E) [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E2A6005D5A7FB7B7	FASTA	955	102,880
	10 20 30 40 50 60 MMATSQDYFG NPYALFRGPP TTLRPRESPL GVGHPHGHGH LHSHAHAHGH GHAHSHYAAL 70 80 90 100 110 120 DLQTPHKRNI ETDVRAPPPP LPPPPLPLPP ASPRYNTDQG AMDQQFCLRW NNHPTNLTGV 130 140 150 160 170 180 LTSLLQREAL CDVTLACEGE TVKAHQTILS ACSPYFETIF LQNQHPHPII YLKDVRYSEM 190 200 210 220 230 240 RSLLDFMYKG EVNVGQSSLP MFLKTAESLQ VRGLTDNNNL NYRSDCDKLR DSAASSPTGR 250 260 270 280 290 300 GPSNYTGGLG GAGGVADAMR ESRDSLRSRC ERDLRDELTQ RSSSSMSERS SAAAAAAAAA 310 320 330 340 350 360 AAVAAAGGNV NAAAVALGLT TPTGGERSPS VGSASAAAAA AAVAAAVAAA ANRSASADGC 370 380 390 400 410 420 SDRGSERGTL ERTDSRDDLL QLDYSNKDNN NSNSSSTGGN NNNNNNNNNN SSSNNNNSSS 430 440 450 460 470 480 NRERNNSGER ERERERERER DRDRELSTTP VEQLSSSKRR RKNSSSNCDN SLSSSHQDRH 490 500 510 520 530 540 YPQDSQANFK SSPVPKTGGS TSESEDAGGR HDSPLSMTTS VHLGGGGGNV GAASALSGLS 550 560 570 580 590 600 QSLSIKQELM DAQQQQQHRE HHVALPPDYL PSAALKLHAE DMSTLLTQHA LQAADARDEH 610 620 630 640 650 660 NDAKQLQLDQ TDNIDGRVKC FNIKHDRHPD RELDRNHREH DDDPGVIEEV VVDHVREMEA 670 680 690 700 710 720 GNEHDPEEMK EAAYHATPPK YRRAVVYAPP HPDEEAASGS GSDIYVDGGY NCEYKCKELN 730 740 750 760 770 780 MRAIRCSRQQ HMMSHYSPHH PHHRSLIDCP AEAAYSPPVA NNQAYLASNG AVQQLDLSTY 790 800 810 820 830 840 HGHANHQLHQ HPPSATHPSH SQSSPHYPSA SGAGAGAGSV SVSIAGSASG SATSAPASVA 850 860 870 880 890 900 TSAVSPQPSS SSTGSTSSAA AVAAAAAAAA NRRDHNIDYS TLFVQLSGTL PTLYRCVSCN 910 920 930 940 950 KIVSNRWHHA NIHRPQSHEC PVCGQKFTRR DNMKAHCKIK HADIKDRFFS HYVHM
	Isoform Female-A (C). Checksum: 3586E2B2CD274996	FASTA	854	91,936
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTGGERSPSV GSASAAAAAA 250 260 270 280 290 300 AVAAAVAAAA NRSASADGCS DRGSERGTLE RTDSRDDLLQ LDYSNKDNNN SNSSSTGGNN 310 320 330 340 350 360 NNNNNNNNNS SSNNNNSSSN RERNNSGERE RERERERERD RDRELSTTPV EQLSSSKRRR 370 380 390 400 410 420 KNSSSNCDNS LSSSHQDRHY PQDSQANFKS SPVPKTGGST SESEDAGGRH DSPLSMTTSV 430 440 450 460 470 480 HLGGGGGNVG AASALSGLSQ SLSIKQELMD AQQQQQHREH HVALPPDYLP SAALKLHAED 490 500 510 520 530 540 MSTLLTQHAL QAADARDEHN DAKQLQLDQT DNIDGRVKCF NIKHDRHPDR ELDRNHREHD 550 560 570 580 590 600 DDPGVIEEVV VDHVREMEAG NEHDPEEMKE AAYHATPPKY RRAVVYAPPH PDEEAASGSG 610 620 630 640 650 660 SDIYVDGGYN CEYKCKELNM RAIRCSRQQH MMSHYSPHHP HHRSLIDCPA EAAYSPPVAN 670 680 690 700 710 720 NQAYLASNGA VQQLDLSTYH GHANHQLHQH PPSATHPSHS QSSPHYPSAS GAGAGAGSVS 730 740 750 760 770 780 VSIAGSASGS ATSAPASVAT SAVSPQPSSS STGSTSSAAA VAAAAAAAAN RRDHNIDYST 790 800 810 820 830 840 LFVQLSGTLP TLYRCVSCNK IVSNRWHHAN IHRPQSHECP VCGQKFTRRD NMKAHCKIKH 850 ADIKDRFFSH YVHM
	Isoform Female-B (F). Checksum: 66338CF48C6BF42C	FASTA	688	73,094
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTGGERSPSV GSASAAAAAA 250 260 270 280 290 300 AVAAAVAAAA NRSASADGCS DRGSERGTLE RTDSRDDLLQ LDYSNKDNNN SNSSSTGGNN 310 320 330 340 350 360 NNNNNNNNNS SSNNNNSSSN RERNNSGERE RERERERERD RDRELSTTPV EQLSSSKRRR 370 380 390 400 410 420 KNSSSNCDNS LSSSHQDRHY PQDSQANFKS SPVPKTGGST SESEDAGGRH DSPLSMTTSV 430 440 450 460 470 480 HLGGGGGNVG AASALSGLSQ SLSIKQELMD AQQQQQHREH HVALPPDYLP SAALKLHAED 490 500 510 520 530 540 MSTLLTQHAL QAADARDEHN DAKQLQLDQT DNIDGSSARH HLSTPLSTSS SASPPPPPFG 550 560 570 580 590 600 MHLSAALKRE YHPLHYMAAG NGHNGPSALG YGNQGSGNAP NSAGGAGSVA GGVGAGGGAG 610 620 630 640 650 660 GATGAAGHNS HHTMSYHNMF TPSRDPGTMW RCRSCGKEVT NRWHHFHSHT AQRSMCPYCP 670 680 ATYSRIDTLR SHLRVKHPDR LLKLNSSI
	Isoform Female-E (H). Checksum: 28B9F9BD279B2DB1	FASTA	695	75,537
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTGGERSPSV GSASAAAAAA 250 260 270 280 290 300 AVAAAVAAAA NRSASADGCS DRGSERGTLE RTDSRDDLLQ LDYSNKDNNN SNSSSTGGNN 310 320 330 340 350 360 NNNNNNNNNS SSNNNNSSSN RERNNSGERE RERERERERD RDRELSTTPV EQLSSSKRRR 370 380 390 400 410 420 KNSSSNCDNS LSSSHQDRHY PQDSQANFKS SPVPKTGGST SESEDAGGRH DSPLSMTTSV 430 440 450 460 470 480 HLGGGGGNVG AASALSGLSQ SLSIKQELMD AQQQQQHREH HVALPPDYLP SAALKLHAED 490 500 510 520 530 540 MSTLLTQHAL QAADARDEHN DAKQLQLDQT DNIDGSKAWH MRLTFERLSG GCNLHRCKLC 550 560 570 580 590 600 GKVVTHIRNH YHVHFPGRFE CPLCRATYTR SDNLRTHCKF KHPMYNPDTR KFDNLMSSSA 610 620 630 640 650 660 VGAAATPTAS QLAVASQAAM AAAAAAAFNA AQQQQQQQQQ QQQHQHQQQQ QHQQSQQQQQ 670 680 690 QQQQSQQQLH ALAQQHMLQL QPQHHQQQQH NATSE
	Isoform Female-I. Checksum: E4772F505C0B0577	FASTA	675	71,950
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTAAAAAAVA AAVAAAANRS 250 260 270 280 290 300 ASADGCSDRG SERGTLERTD SRDDLLQLDY SNKDNNNSNS SSTGGNNNNN NNNNNNSSSN 310 320 330 340 350 360 NNNSSSNRER NNSGERERER ERERERDRDR ELSTTPVEQL SSSKRRRKNS SSNCDNSLSS 370 380 390 400 410 420 SHQDRHYPQD SQANFKSSPV PKTGGSTSES EDAGGRHDSP LSMTTSVHLG GGGGNVGAAS 430 440 450 460 470 480 ALSGLSQSLS IKQELMDAQQ QQQHREHHVA LPPDYLPSAA LKLHAEDMST LLTQHALQAA 490 500 510 520 530 540 DARDEHNDAK QLQLDQTDNI DGSSARHHLS TPLSTSSSAS PPPPPFGMHL SAALKREYHP 550 560 570 580 590 600 LHYMAAGNGH NGPSALGYGN QGSGNAPNSA GGAGSVAGGV GAGGGAGGAT GAAGHNSHHT 610 620 630 640 650 660 MSYHNMFTPS RDPGTMWRCR SCGKEVTNRW HHFHSHTAQR SMCPYCPATY SRIDTLRSHL 670 RVKHPDRLLK LNSSI
	Isoform Male-B (B). Checksum: 7F87220F6C723A29	FASTA	789	84,038
	10 20 30 40 50 60 MMATSQDYFG NPYALFRGPP TTLRPRESPL GVGHPHGHGH LHSHAHAHGH GHAHSHYAAL 70 80 90 100 110 120 DLQTPHKRNI ETDVRAPPPP LPPPPLPLPP ASPRYNTDQG AMDQQFCLRW NNHPTNLTGV 130 140 150 160 170 180 LTSLLQREAL CDVTLACEGE TVKAHQTILS ACSPYFETIF LQNQHPHPII YLKDVRYSEM 190 200 210 220 230 240 RSLLDFMYKG EVNVGQSSLP MFLKTAESLQ VRGLTDNNNL NYRSDCDKLR DSAASSPTGR 250 260 270 280 290 300 GPSNYTGGLG GAGGVADAMR ESRDSLRSRC ERDLRDELTQ RSSSSMSERS SAAAAAAAAA 310 320 330 340 350 360 AAVAAAGGNV NAAAVALGLT TPTGGERSPS VGSASAAAAA AAVAAAVAAA ANRSASADGC 370 380 390 400 410 420 SDRGSERGTL ERTDSRDDLL QLDYSNKDNN NSNSSSTGGN NNNNNNNNNN SSSNNNNSSS 430 440 450 460 470 480 NRERNNSGER ERERERERER DRDRELSTTP VEQLSSSKRR RKNSSSNCDN SLSSSHQDRH 490 500 510 520 530 540 YPQDSQANFK SSPVPKTGGS TSESEDAGGR HDSPLSMTTS VHLGGGGGNV GAASALSGLS 550 560 570 580 590 600 QSLSIKQELM DAQQQQQHRE HHVALPPDYL PSAALKLHAE DMSTLLTQHA LQAADARDEH 610 620 630 640 650 660 NDAKQLQLDQ TDNIDGSSAR HHLSTPLSTS SSASPPPPPF GMHLSAALKR EYHPLHYMAA 670 680 690 700 710 720 GNGHNGPSAL GYGNQGSGNA PNSAGGAGSV AGGVGAGGGA GGATGAAGHN SHHTMSYHNM 730 740 750 760 770 780 FTPSRDPGTM WRCRSCGKEV TNRWHHFHSH TAQRSMCPYC PATYSRIDTL RSHLRVKHPD RLLKLNSSI
	Isoform Male-E (G). Checksum: DB9F4F6F30170AD8	FASTA	796	86,481
	10 20 30 40 50 60 MMATSQDYFG NPYALFRGPP TTLRPRESPL GVGHPHGHGH LHSHAHAHGH GHAHSHYAAL 70 80 90 100 110 120 DLQTPHKRNI ETDVRAPPPP LPPPPLPLPP ASPRYNTDQG AMDQQFCLRW NNHPTNLTGV 130 140 150 160 170 180 LTSLLQREAL CDVTLACEGE TVKAHQTILS ACSPYFETIF LQNQHPHPII YLKDVRYSEM 190 200 210 220 230 240 RSLLDFMYKG EVNVGQSSLP MFLKTAESLQ VRGLTDNNNL NYRSDCDKLR DSAASSPTGR 250 260 270 280 290 300 GPSNYTGGLG GAGGVADAMR ESRDSLRSRC ERDLRDELTQ RSSSSMSERS SAAAAAAAAA 310 320 330 340 350 360 AAVAAAGGNV NAAAVALGLT TPTGGERSPS VGSASAAAAA AAVAAAVAAA ANRSASADGC 370 380 390 400 410 420 SDRGSERGTL ERTDSRDDLL QLDYSNKDNN NSNSSSTGGN NNNNNNNNNN SSSNNNNSSS 430 440 450 460 470 480 NRERNNSGER ERERERERER DRDRELSTTP VEQLSSSKRR RKNSSSNCDN SLSSSHQDRH 490 500 510 520 530 540 YPQDSQANFK SSPVPKTGGS TSESEDAGGR HDSPLSMTTS VHLGGGGGNV GAASALSGLS 550 560 570 580 590 600 QSLSIKQELM DAQQQQQHRE HHVALPPDYL PSAALKLHAE DMSTLLTQHA LQAADARDEH 610 620 630 640 650 660 NDAKQLQLDQ TDNIDGSKAW HMRLTFERLS GGCNLHRCKL CGKVVTHIRN HYHVHFPGRF 670 680 690 700 710 720 ECPLCRATYT RSDNLRTHCK FKHPMYNPDT RKFDNLMSSS AVGAAATPTA SQLAVASQAA 730 740 750 760 770 780 MAAAAAAAFN AAQQQQQQQQ QQQQHQHQQQ QQHQQSQQQQ QQQQQSQQQL HALAQQHMLQ 790 LQPQHHQQQQ HNATSE
	Isoform Male-I. Checksum: BE4560B1E0DF6297	FASTA	776	82,895
	10 20 30 40 50 60 MMATSQDYFG NPYALFRGPP TTLRPRESPL GVGHPHGHGH LHSHAHAHGH GHAHSHYAAL 70 80 90 100 110 120 DLQTPHKRNI ETDVRAPPPP LPPPPLPLPP ASPRYNTDQG AMDQQFCLRW NNHPTNLTGV 130 140 150 160 170 180 LTSLLQREAL CDVTLACEGE TVKAHQTILS ACSPYFETIF LQNQHPHPII YLKDVRYSEM 190 200 210 220 230 240 RSLLDFMYKG EVNVGQSSLP MFLKTAESLQ VRGLTDNNNL NYRSDCDKLR DSAASSPTGR 250 260 270 280 290 300 GPSNYTGGLG GAGGVADAMR ESRDSLRSRC ERDLRDELTQ RSSSSMSERS SAAAAAAAAA 310 320 330 340 350 360 AAVAAAGGNV NAAAVALGLT TPTAAAAAAV AAAVAAAANR SASADGCSDR GSERGTLERT 370 380 390 400 410 420 DSRDDLLQLD YSNKDNNNSN SSSTGGNNNN NNNNNNNSSS NNNNSSSNRE RNNSGERERE 430 440 450 460 470 480 RERERERDRD RELSTTPVEQ LSSSKRRRKN SSSNCDNSLS SSHQDRHYPQ DSQANFKSSP 490 500 510 520 530 540 VPKTGGSTSE SEDAGGRHDS PLSMTTSVHL GGGGGNVGAA SALSGLSQSL SIKQELMDAQ 550 560 570 580 590 600 QQQQHREHHV ALPPDYLPSA ALKLHAEDMS TLLTQHALQA ADARDEHNDA KQLQLDQTDN 610 620 630 640 650 660 IDGSSARHHL STPLSTSSSA SPPPPPFGMH LSAALKREYH PLHYMAAGNG HNGPSALGYG 670 680 690 700 710 720 NQGSGNAPNS AGGAGSVAGG VGAGGGAGGA TGAAGHNSHH TMSYHNMFTP SRDPGTMWRC 730 740 750 760 770 RSCGKEVTNR WHHFHSHTAQ RSMCPYCPAT YSRIDTLRSH LRVKHPDRLL KLNSSI
	Isoform Type-C (D). Checksum: 2235D16063D4444C	FASTA	665	72,365
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTGGERSPSV GSASAAAAAA 250 260 270 280 290 300 AVAAAVAAAA NRSASADGCS DRGSERGTLE RTDSRDDLLQ LDYSNKDNNN SNSSSTGGNN 310 320 330 340 350 360 NNNNNNNNNS SSNNNNSSSN RERNNSGERE RERERERERD RDRELSTTPV EQLSSSKRRR 370 380 390 400 410 420 KNSSSNCDNS LSSSHQDRHY PQDSQANFKS SPVPKTGGST SESEDAGGRH DSPLSMTTSV 430 440 450 460 470 480 HLGGGGGNVG AASALSGLSQ SLSIKQELMD AQQQQQHREH HVALPPDYLP VKKSEAFLGS 490 500 510 520 530 540 TGNKSMHQML LHQAVEAQLK SFQLHYQNEG LDSAMHRLLA QQQQHQEQQQ QHQQQPHHSL 550 560 570 580 590 600 GKSQSPAIPS GSAGGSSRKS GRFRANWLYQ FEWLQYDERA NTMFCRHCRK WSGELADIRT 610 620 630 640 650 660 SFVEGNSNFR LEIVNHHNKC KSHRMCYERE LQEQQQHPMP SGSAGGSSKR RSPEIITINV GKNSA
	Isoform Type-D (A). Checksum: E91B5D22099FF65B	FASTA	516	55,086
	10 20 30 40 50 60 MDQQFCLRWN NHPTNLTGVL TSLLQREALC DVTLACEGET VKAHQTILSA CSPYFETIFL 70 80 90 100 110 120 QNQHPHPIIY LKDVRYSEMR SLLDFMYKGE VNVGQSSLPM FLKTAESLQV RGLTDNNNLN 130 140 150 160 170 180 YRSDCDKLRD SAASSPTGRG PSNYTGGLGG AGGVADAMRE SRDSLRSRCE RDLRDELTQR 190 200 210 220 230 240 SSSSMSERSS AAAAAAAAAA AVAAAGGNVN AAAVALGLTT PTGGERSPSV GSASAAAAAA 250 260 270 280 290 300 AVAAAVAAAA NRSASADGCS DRGSERGTLE RTDSRDDLLQ LDYSNKDNNN SNSSSTGGNN 310 320 330 340 350 360 NNNNNNNNNS SSNNNNSSSN RERNNSGERE RERERERERD RDRELSTTPV EQLSSSKRRR 370 380 390 400 410 420 KNSSSNCDNS LSSSHQDRHY PQDSQANFKS SPVPKTGGST SESEDAGGRH DSPLSMTTSV 430 440 450 460 470 480 HLGGGGGNVG AASALSGLSQ SLSIKQELMD AQQQQQHREH HVALPPDYLP SAALKLHAED 490 500 510 MSTLLTQHAL QAADARDEHN DAKQLQLDQT DNIDGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of male sexual behavior and sexual orientation in Drosophila by the fruitless gene." Ryner L.C., Goodwin S.F., Castrillon D.H., Anand A., Villella A., Baker B.S., Hall J.C., Taylor B.J., Wasserman S.A. Cell 87:1079-1089(1996) [PubMed: 8978612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MALE-I AND FEMALE-I), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH TRA, DISRUPTION PHENOTYPE. Strain: Oregon-R.
[2]	"Sexual orientation in Drosophila is altered by the satori mutation in the sex-determination gene fruitless that encodes a zinc finger protein with a BTB domain." Ito H., Fujitani K., Usui K., Shimizu-Nishikawa K., Tanaka S., Yamamoto D. Proc. Natl. Acad. Sci. U.S.A. 93:9687-9692(1996) [PubMed: 8790392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FEMALE-A), FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Head.
[3]	"Formation of the male-specific muscle in female Drosophila by ectopic fruitless expression." Usui-Aoki K., Ito H., Ui-Tei K., Takahashi K., Lukacsovich T., Awano W., Nakata H., Piao Z.F., Nilsson E.E., Tomida J.-Y., Yamamoto D. Nat. Cell Biol. 2:500-506(2000) [PubMed: 10934470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MALE-A; FEMALE-A; MALE-B; FEMALE-B; TYPE-C; TYPE-D; MALE-E AND FEMALE-E), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH TRA. Strain: Canton-S. Tissue: Head.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[6]	"The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila." Zollman S., Godt D., Prive G.G., Couderc J.-L., Laski F.A. Proc. Natl. Acad. Sci. U.S.A. 91:10717-10721(1994) [PubMed: 7938017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-218.
[7]	"Regulation of sex-specific selection of fruitless 5' splice sites by transformer and transformer-2." Heinrichs V., Ryner L.C., Baker B.S. Mol. Cell. Biol. 18:450-458(1998) [PubMed: 9418892] [Abstract] Cited for: FUNCTION.
[8]	"The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior." Dauwalder B., Tsujimoto S., Moss J., Mattox W. Genes Dev. 16:2879-2892(2002) [PubMed: 12435630] [Abstract] Cited for: FUNCTION. Strain: Canton-S.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF039231 mRNA. Translation: AAB96677.1. U72492 mRNA. Translation: AAB92662.1. D84437 mRNA. Translation: BAA12663.1. D84438 mRNA. Translation: BAA12664.1. AF220176 mRNA. Translation: AAG28587.1. AF220177 mRNA. Translation: AAG28588.1. AF220178 mRNA. Translation: AAG28589.1. AF220179 mRNA. Translation: AAG28590.1. AF220180 mRNA. Translation: AAG28591.1. AF220181 mRNA. Translation: AAG28592.1. AE014297 Genomic DNA. Translation: AAF55562.2. AE014297 Genomic DNA. Translation: AAF55563.2. AE014297 Genomic DNA. Translation: AAF55564.2. AE014297 Genomic DNA. Translation: AAF55565.2. AE014297 Genomic DNA. Translation: AAN13774.1. AE014297 Genomic DNA. Translation: AAN13775.1. AE014297 Genomic DNA. Translation: AAN13776.1. AE014297 Genomic DNA. Translation: AAN13777.1. U14403 Genomic DNA. Translation: AAA50838.1.
RefSeq	NP_524397.2. NP_732344.1. NP_732345.1. NP_732346.1. NP_732347.1. NP_732348.1. NP_732349.1. NP_732350.1.
UniGene	Dm.4626
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17120N.
IntAct	Q8IN81. 8 interactions.
Proteomic databases
PRIDE	Q8IN81.
Genome annotation databases
Ensembl	FBgn0004652. Drosophila melanogaster. [Contig view]
GeneID	42226.
KEGG	dme:Dmel_CG14307.
Organism-specific databases
FlyBase	FBgn0004652. fru.
Phylogenomic databases
OMA	Q8IN81. RSCGKEV.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-012238-MON.
Gene expression databases
ArrayExpress	Q8IN81.
GermOnline	CG14307. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	827755.

Entry information

Entry name

FRU_DROME

Accession

Primary (citable) accession number: Q8IN81
Secondary accession number(s): O44708 , P91618, P91619, Q24004, Q8IN80, Q8IN82, Q8IN83, Q9GU18, Q9GU19, Q9GU20, Q9GU21, Q9GU22, Q9VE64, Q9VE65, Q9VE66, Q9VE67

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project