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Protein

Mitochondrial Rho GTPase

Gene

Miro

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase involved in mitochondrial trafficking. Milt and Miro form an essential protein complex that links Khc to mitochondria for light chain-independent, anterograde transport of mitochondria. Required for axonal transport to synapses within nerve terminals. Required presynaptically but not postsynaptically at neuromuscular junctions (NMJs).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 25GTP 1Sequence analysis8
Nucleotide bindingi64 – 68GTP 1Sequence analysis5
Nucleotide bindingi144 – 147GTP 1Sequence analysis4
Calcium bindingi223 – 2341PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi343 – 3542PROSITE-ProRule annotationAdd BLAST12
Nucleotide bindingi453 – 460GTP 2Sequence analysis8
Nucleotide bindingi491 – 495GTP 2Sequence analysis5
Nucleotide bindingi558 – 561GTP 2Sequence analysis4

GO - Molecular functioni

  • calcium ion binding Source: FlyBase
  • GTPase activity Source: FlyBase
  • GTP binding Source: FlyBase
  • magnesium ion binding Source: FlyBase

GO - Biological processi

  • cellular homeostasis Source: UniProtKB
  • establishment of mitochondrion localization, microtubule-mediated Source: FlyBase
  • microtubule cytoskeleton organization Source: FlyBase
  • mitochondrial outer membrane permeabilization Source: UniProtKB
  • mitochondrion localization Source: FlyBase
  • mitochondrion transport along microtubule Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
  • synaptic vesicle transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, GTP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-194840. Rho GTPase cycle.
R-DME-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial Rho GTPase (EC:3.6.5.-)
Short name:
Miro
Short name:
dMiro
Gene namesi
Name:Miro
ORF Names:CG5410
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039140. Miro.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 621Mitochondrial intermembraneSequence analysisAdd BLAST621
Transmembranei622 – 641Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini642 – 652CytoplasmicSequence analysisAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Flies die at early pupal stage and exhibit abnormal locomotion. Mitochondria in muscles and neurons are abnormally distributed. Instead of being transported into axons and dendrites, mitochondria accumulate in parallel rows in neuronal somata. Mutant NMJs lack presynaptic mitochondria, but neurotransmitter release and acute Ca2+ buffering is only impaired during prolonged stimulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393271 – 652Mitochondrial Rho GTPaseAdd BLAST652

Proteomic databases

PaxDbiQ8IMX7.
PRIDEiQ8IMX7.

Expressioni

Gene expression databases

BgeeiFBgn0039140.
ExpressionAtlasiQ8IMX7. baseline.
GenevisibleiQ8IMX7. DM.

Interactioni

Subunit structurei

Interacts with kinesin-associated protein milt.2 Publications

Protein-protein interaction databases

BioGridi67780. 20 interactors.
IntActiQ8IMX7. 6 interactors.
MINTiMINT-337818.
STRINGi7227.FBpp0302814.

Structurei

Secondary structure

1652
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi209 – 222Combined sources14
Beta strandi226 – 228Combined sources3
Helixi232 – 243Combined sources12
Helixi249 – 262Combined sources14
Beta strandi266 – 268Combined sources3
Helixi274 – 284Combined sources11
Turni285 – 288Combined sources4
Helixi290 – 299Combined sources10
Beta strandi306 – 308Combined sources3
Helixi310 – 313Combined sources4
Beta strandi324 – 327Combined sources4
Helixi329 – 341Combined sources13
Beta strandi347 – 350Combined sources4
Helixi352 – 358Combined sources7
Helixi359 – 361Combined sources3
Beta strandi362 – 364Combined sources3
Turni369 – 372Combined sources4
Helixi373 – 376Combined sources4
Turni381 – 383Combined sources3
Helixi388 – 401Combined sources14
Helixi403 – 413Combined sources11
Helixi415 – 419Combined sources5
Helixi423 – 426Combined sources4
Beta strandi427 – 430Combined sources4
Beta strandi446 – 452Combined sources7
Helixi459 – 464Combined sources6
Helixi465 – 467Combined sources3
Helixi471 – 474Combined sources4
Beta strandi484 – 492Combined sources9
Beta strandi495 – 503Combined sources9
Beta strandi509 – 511Combined sources3
Helixi515 – 518Combined sources4
Beta strandi520 – 530Combined sources11
Turni532 – 534Combined sources3
Helixi535 – 545Combined sources11
Turni546 – 548Combined sources3
Beta strandi549 – 551Combined sources3
Beta strandi553 – 558Combined sources6
Beta strandi568 – 571Combined sources4
Helixi573 – 579Combined sources7
Beta strandi586 – 588Combined sources3
Beta strandi590 – 592Combined sources3
Helixi596 – 606Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0JX-ray2.82A201-617[»]
4C0KX-ray2.80A201-617[»]
4C0LX-ray3.00A201-617[»]
ProteinModelPortaliQ8IMX7.
SMRiQ8IMX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 168Miro 1Add BLAST164
Domaini210 – 245EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini330 – 365EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini440 – 607Miro 2Add BLAST168

Sequence similaritiesi

Belongs to the mitochondrial Rho GTPase family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 Miro domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1707. Eukaryota.
ENOG410XRHW. LUCA.
GeneTreeiENSGT00390000014374.
InParanoidiQ8IMX7.
KOiK07870.
OMAiAIMEDYP.
OrthoDBiEOG091G04UF.
PhylomeDBiQ8IMX7.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR013566. EF_hand_assoc_1.
IPR013567. EF_hand_assoc_2.
IPR002048. EF_hand_dom.
IPR020860. MIRO_dom.
IPR021181. Mt_Rho_GTPase.
IPR027417. P-loop_NTPase.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF08355. EF_assoc_1. 1 hit.
PF08356. EF_assoc_2. 1 hit.
PF00071. Ras. 2 hits.
[Graphical view]
PIRSFiPIRSF037488. Mt_Rho_GTPase. 1 hit.
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51423. MIRO. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q8IMX7-1) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQYTASQRK NVRILLVGDA GVGKTSLILS LVSEEYPEEV PPRAEEITIP
60 70 80 90 100
ANVTPEQVPT SIVDFSAVEQ SEDALAAEIN KAHVVCIVYA VDDDDTLDRI
110 120 130 140 150
TSHWLPLVRA KCNPSLDGEG DAEAEAEGDT QREPIRKPIV LVGNKIDLIE
160 170 180 190 200
YSTMDSVLAI MEDYPEIESC VECSAKSLHN ISEMFYYAQK AVLHPTSPLY
210 220 230 240 250
MMEEQELTSA CKKSLVRIFK ICDIDGDNLL NDYELNLFQR RCFNTPLQPQ
260 270 280 290 300
ILDEVKAVIQ KNVPDGIYND AVTLKGFLFL HCLFIQRGRN ETTWAVLRRF
310 320 330 340 350
GYNDQLEMCQ EYLRPPLKIP PGSSTELSHR GQQFLIAVFE RYDRDGDGAL
360 370 380 390 400
SPEEHKMLFS TCPAAPWSYS TDIRKSCPIN ETTGWVTLHG WLCRWTLMTL
410 420 430 440 450
IDVVKTMEYL AYLGFNVHEN DSQLAAIHVT RERRIDLAKR QSSRSVYKCH
460 470 480 490 500
VIGPKGSGKT GMCRGFLVED MHKLIGKEFK TNVVNCINSV QVYGQEKHLI
510 520 530 540 550
LRDIDVRHAL DPLQPQEVNC DVACLVYDSS NPRSFEYVAR IYIKYYAESK
560 570 580 590 600
IPVMIVGTKC DMDERRQDYL MQPSEFCDKY KLLPPHLFSL KTNKKELYTK
610 620 630 640 650
LATMAAFPHL RQFGLMTEDP KLWLKAGLGV AAATMLGFIV LKTISAAGAH

TR
Length:652
Mass (Da):73,776
Last modified:March 1, 2003 - v1
Checksum:i5F2F9623EF6CCB19
GO
Isoform B (identifier: Q8IMX7-2) [UniParc]FASTAAdd to basket
Also known as: E

The sequence of this isoform differs from the canonical sequence as follows:
     608-608: P → PRFQAAWILFYKHRLVQLWESA

Show »
Length:673
Mass (Da):76,421
Checksum:iAC21606FC66B03AF
GO

Sequence cautioni

The sequence AAL13784 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019165608P → PRFQAAWILFYKHRLVQLWE SA in isoform B. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13971.1.
AE014297 Genomic DNA. Translation: AAN13972.1.
BT016011 mRNA. Translation: AAV36896.1.
AY058555 mRNA. Translation: AAL13784.1. Different initiation.
RefSeqiNP_001262895.1. NM_001275966.1. [Q8IMX7-2]
NP_651205.2. NM_142948.4. [Q8IMX7-2]
NP_732936.1. NM_170111.3. [Q8IMX7-1]
UniGeneiDm.7314.

Genome annotation databases

EnsemblMetazoaiFBtr0084595; FBpp0083980; FBgn0039140. [Q8IMX7-1]
GeneIDi42845.
KEGGidme:Dmel_CG5410.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13971.1.
AE014297 Genomic DNA. Translation: AAN13972.1.
BT016011 mRNA. Translation: AAV36896.1.
AY058555 mRNA. Translation: AAL13784.1. Different initiation.
RefSeqiNP_001262895.1. NM_001275966.1. [Q8IMX7-2]
NP_651205.2. NM_142948.4. [Q8IMX7-2]
NP_732936.1. NM_170111.3. [Q8IMX7-1]
UniGeneiDm.7314.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0JX-ray2.82A201-617[»]
4C0KX-ray2.80A201-617[»]
4C0LX-ray3.00A201-617[»]
ProteinModelPortaliQ8IMX7.
SMRiQ8IMX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67780. 20 interactors.
IntActiQ8IMX7. 6 interactors.
MINTiMINT-337818.
STRINGi7227.FBpp0302814.

Proteomic databases

PaxDbiQ8IMX7.
PRIDEiQ8IMX7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084595; FBpp0083980; FBgn0039140. [Q8IMX7-1]
GeneIDi42845.
KEGGidme:Dmel_CG5410.

Organism-specific databases

CTDi42845.
FlyBaseiFBgn0039140. Miro.

Phylogenomic databases

eggNOGiKOG1707. Eukaryota.
ENOG410XRHW. LUCA.
GeneTreeiENSGT00390000014374.
InParanoidiQ8IMX7.
KOiK07870.
OMAiAIMEDYP.
OrthoDBiEOG091G04UF.
PhylomeDBiQ8IMX7.

Enzyme and pathway databases

ReactomeiR-DME-194840. Rho GTPase cycle.
R-DME-5689880. Ub-specific processing proteases.

Miscellaneous databases

GenomeRNAii42845.
PROiQ8IMX7.

Gene expression databases

BgeeiFBgn0039140.
ExpressionAtlasiQ8IMX7. baseline.
GenevisibleiQ8IMX7. DM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR013566. EF_hand_assoc_1.
IPR013567. EF_hand_assoc_2.
IPR002048. EF_hand_dom.
IPR020860. MIRO_dom.
IPR021181. Mt_Rho_GTPase.
IPR027417. P-loop_NTPase.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF08355. EF_assoc_1. 1 hit.
PF08356. EF_assoc_2. 1 hit.
PF00071. Ras. 2 hits.
[Graphical view]
PIRSFiPIRSF037488. Mt_Rho_GTPase. 1 hit.
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51423. MIRO. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIRO_DROME
AccessioniPrimary (citable) accession number: Q8IMX7
Secondary accession number(s): Q8IMX6, Q95TT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.