Q8IMA8 (PANG2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein pangolin, isoform J

Alternative name(s):
dTCF

Gene names

Name:	pan
Synonyms:	TCF
ORF Names:	CG34403

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1192 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Segment polarity protein. Functions together with arm to transduce the Wingless (Wg) signal in embryos and in developing adult tissues. Acts as a transcriptional activator, but in the absence of arm, it binds to gro and acts as a transcriptional repressor of wg-responsive genes. Ref.5 Ref.6 Ref.7
Subunit structure	Binds to the beta-catenin homolog arm or to gro. Ref.7
Subcellular location	Nucleus Ref.7.
Disruption phenotype	Flies exhibit a segment polarity phenotype and altered expression of the wg target genes en and Ubx. Ref.5
Sequence similarities	Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Developmental protein Repressor Segmentation polarity protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome heart development Traceable author statement. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB mesodermal cell fate determination Traceable author statement. Source: FlyBase negative regulation of Wnt receptor signaling pathway Non-traceable author statement. Source: FlyBase negative regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.5. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of hemocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of stem cell proliferation Inferred from mutant phenotype. Source: FlyBase regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome salivary gland morphogenesis Inferred from mutant phenotype. Source: FlyBase segment polarity determination Inferred from electronic annotation. Source: UniProtKB-KW spiracle morphogenesis, open tracheal system Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	microtubule associated complex Inferred from direct assay. Source: FlyBase transcription factor complex Inferred from physical interaction. Source: FlyBase
Molecular function	beta-catenin binding Inferred from Biological aspect of Ancestor. Source: RefGenome kinase binding Inferred from physical interaction. Source: FlyBase repressing transcription factor binding Inferred from physical interaction. Source: FlyBase sequence-specific DNA binding Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform J Ref.1 (identifier: Q8IMA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: P91943-3)

Also known as: C; D; E; F; G;

The sequence of this isoform can be found in the external entry P91943.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform H Ref.1 (identifier: P91943-4)

The sequence of this isoform can be found in the external entry P91943.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform I Ref.1 (identifier: P91943-5)

The sequence of this isoform can be found in the external entry P91943.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1192	1192	Protein pangolin, isoform J		PRO_0000322557
Regions
	DNA binding	714 – 782	69	HMG box
	Motif	351 – 357	7	Nuclear localization signal Potential
	Compositional bias	197 – 200	4	Poly-Asn
	Compositional bias	489 – 494	6	Poly-Ser
	Compositional bias	792 – 798	7	Poly-Lys

Sequences

	Sequence		Length	Mass (Da)
	Isoform J [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 173ED73F8673DAA6	FASTA	1,192	132,080
	10 20 30 40 50 60 MPHCGTTNSE INLNSNELIS YQKKKSNEDL QKKHDKKCSI NLKTSQVPDG GFNNQYTQLT 70 80 90 100 110 120 ANKIEQYNCN DLDKVCIVPS SRSDGMEASF SELLLSPNKL ISQPWQTAEE IENWQNDSFR 130 140 150 160 170 180 QRNEMFSCIY TNSMLNQQPC SQQQLLATQL LYARLLRSQL AEREFHSNKF NMVHYSGSKK 190 200 210 220 230 240 TMLREDELLS TPSSQDNNNN IKLIKDIENS ISCVDPPLFE FSNVHQRAEQ KNKQDDKNCY 250 260 270 280 290 300 SPKLKSNKEA LDGYDLQHTC DFIREQKNIL IDIKKKLDNL SDSSGKFRKR LSVRQSHIEV 310 320 330 340 350 360 NNGSNSALEE SVRRQLNGNR KSIENLLEEV KRLYNQWSSA ELYYVRSLQR LGLPSEEDGE 370 380 390 400 410 420 YTSTPTHTIM ALAAIALSNE SILPQKTNAV HSKIPDVESE SDFFLTSAKP KTLVEIEDII 430 440 450 460 470 480 LQLASSVNMH QSSSASTPHE SYSDSVKSDC EESNSAPTCI WHSTRQTFRH KKDVEPCSTA 490 500 510 520 530 540 AEIILEYASL SSSSNIETSR LLTSASNLTD VTENYNVTTQ LPIVFNYNRE SSAESIVTDP 550 560 570 580 590 600 LLVPEFSTAP STPSTGSNSG CSTGMVSGIF GLSQNRRKQR LARHIETLTT NSFKSNAIRG 610 620 630 640 650 660 NVDNEITNQL KTSPSIRALP TENVSHLITK TLSSANASLT AQELSITNLF KERLCALQGN 670 680 690 700 710 720 AGSMKTEIFP MIDAPYDLSI GSKTKHMNLE AKHTSNAQSN ESKETTNDKK KPHIKKPLNA 730 740 750 760 770 780 FMLYMKEMRA KVVAECTLKE SAAINQILGR RWHELSREEQ SKYYEKARQE RQLHMELYPG 790 800 810 820 830 840 WSARDNYGYV SKKKKRKKDR STTDSGGNNM KKCRARFGLD QQSQWCKPCR RKKKCIRYME 850 860 870 880 890 900 ALNGNGPAED GSCFDEHGSQ LSDDDEDDYD DDKLGGSCGS ADETNKIEDE DSESLNQSMP 910 920 930 940 950 960 SPGCLSGLSS LQSPSTTMSL ASPLNMNANS ATNVIFPASS NALLIVGADQ PTAQQRPTLV 970 980 990 1000 1010 1020 STSGSSSGST SSISTTPNTS STVSPVTCMT GPCLGSSQER AMMLGNRFSH LGMGLSPPVV 1030 1040 1050 1060 1070 1080 STSTSKSEPF FKPHPTVCNN PIFALPSIGN CSLNISSMPN TSRNPIGANP RDINNPLSIN 1090 1100 1110 1120 1130 1140 QLTKRREYKN VELIEASESK TIVAHAATSI IQHVAVNGYH ANHSLLNSNL GHLHHQLNNR 1150 1160 1170 1180 1190 TENPNRSEQT MLSVSNHSVN SSECHKESDS QAIVSSNPPN AGSSDNGVIS VS
	Isoform A (C) (D) (E) (F) (G) [UniParc]. See P91943.		–	–
	Isoform H [UniParc]. See P91943.		–	–
	Isoform I [UniParc]. See P91943.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 804-1192. Strain: Berkeley.
[4]	"Recombination, dominance and selection on amino acid polymorphism in the Drosophila genome: contrasting patterns on the X and fourth chromosomes." Sheldahl L.A., Weinreich D.M., Rand D.M. Genetics 165:1195-1208(2003) [PubMed: 14668375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 831-1192. Strain: Crete24, Crete26, Crete30, Crete31, Crete35, Crete40, Crete42, Crete43, Crete44, Crete8, FTF1, FTF105, FTF14, FTF2, FTF20, FTF23, FTF26, FTF28, FTF5, FTF6, Zim11, Zim2, Zim30 and Zim53.
[5]	"Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF." van de Wetering M., Cavallo R.A., Dooijes D., van Beest M., van Es J., Loureiro J., Ypma A., Hursh D., Jones T., Bejsovec A., Peifer M., Mortin M., Clevers H. Cell 88:789-799(1997) [PubMed: 9118222] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to transduce the Wingless signal in Drosophila." Brunner E., Peter O., Schweizer L., Basler K. Nature 385:829-833(1997) [PubMed: 9039917] [Abstract] Cited for: FUNCTION.
[7]	"Drosophila Tcf and Groucho interact to repress Wingless signalling activity." Cavallo R.A., Cox R.T., Moline M.M., Roose J., Polevoy G.A., Clevers H., Peifer M., Bejsovec A. Nature 395:604-608(1998) [PubMed: 9783586] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARM AND GRO, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014135 Genomic DNA. Translation: AAN06549.2. BT029282 mRNA. Translation: ABK30919.1. AY312729 Genomic DNA. Translation: AAQ67543.1. AY312730 Genomic DNA. Translation: AAQ67544.1. AY312731 Genomic DNA. Translation: AAQ67545.1. AY312732 Genomic DNA. Translation: AAQ67546.1. AY312733 Genomic DNA. Translation: AAQ67547.1. AY312734 Genomic DNA. Translation: AAQ67548.1. AY312735 Genomic DNA. Translation: AAQ67549.1. AY312736 Genomic DNA. Translation: AAQ67550.1. AY312737 Genomic DNA. Translation: AAQ67551.1. AY312738 Genomic DNA. Translation: AAQ67552.1. AY312739 Genomic DNA. Translation: AAQ67553.1. AY312740 Genomic DNA. Translation: AAQ67554.1. AY312741 Genomic DNA. Translation: AAQ67555.1. AY312742 Genomic DNA. Translation: AAQ67556.1. AY312743 Genomic DNA. Translation: AAQ67557.1. AY312744 Genomic DNA. Translation: AAQ67558.1. AY312745 Genomic DNA. Translation: AAQ67559.1. AY312746 Genomic DNA. Translation: AAQ67560.1. AY312747 Genomic DNA. Translation: AAQ67561.1. AY312748 Genomic DNA. Translation: AAQ67562.1. AY312749 Genomic DNA. Translation: AAQ67563.1. AY312750 Genomic DNA. Translation: AAQ67564.1. AY312751 Genomic DNA. Translation: AAQ67565.1. AY312752 Genomic DNA. Translation: AAQ67566.1.
RefSeq	NP_726528.2. NM_166724.2.
UniGene	Dm.17803.
3D structure databases
ProteinModelPortal	Q8IMA8.
SMR	Q8IMA8. Positions 713-788.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8IMA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0112657; FBpp0111569; FBgn0085432.
GeneID	43769.
KEGG	dme:Dmel_CG34403.
Organism-specific databases
CTD	43769.
FlyBase	FBgn0085432. pan.
Phylogenomic databases
GeneTree	EMGT00050000002014.
InParanoid	Q8IMA8.
PhylomeDB	Q8IMA8.
Gene expression databases
ArrayExpress	Q8IMA8.
Bgee	Q8IMA8.
Family and domain databases
InterPro	IPR000910. HMG_HMG1/HMG2. IPR009071. HMG_superfamily. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 1 hit.
Pfam	PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 1 hit. [Graphical view]
SUPFAM	SSF47095. HMG-box. 1 hit.
PROSITE	PS50118. HMG_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	835691.

Entry information

Entry name

PANG2_DROME

Accession

Primary (citable) accession number: Q8IMA8
Secondary accession number(s): A0AVW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project