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Protein

Bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase

Gene

PF14_0511

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which catalyzes the first two steps of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.1 Publication

Catalytic activityi

6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.1 Publication
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.1 Publication

Enzyme regulationi

G6PD activity is inhibited by glucosamine-6-phosphate, NADPH, and 4-(4-bromophenyl)-7-(3,4-dimethoxyphenyl)-4,6,7,8-tetrahydroquinoline-2,5(1 H,3H)-dione. G6PD and 6PGL activities can be reversibly inhibited by S-glutathionylation (in vitro).1 Publication

Kineticsi

  1. KM=19.2 µM for glucose-6-phosphate (at 25 degrees Celsius)1 Publication
  2. KM=6.5 µM for NADP+ (at 25 degrees Celsius)1 Publication
  3. KM=172 µM for 6-phosphoglucono-gamma-lactone (at 25 degrees Celsius)1 Publication
  1. Vmax=5.2 µmol/min/mg enzyme towards glucose-6-phosphate (at 25 degrees Celsius)1 Publication
  2. Vmax=4.6 µmol/min/mg enzyme towards NADP+ (at 25 degrees Celsius)1 Publication
  3. Vmax=46.6 µmol/min/mg enzyme with 6-phosphoglucono-gamma-lactone as substrate (at 25 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.0 for G6PD activity.1 Publication

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase (PF14_0511)
  2. Bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase (PF14_0511)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (PF14_0520)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei379NADPBy similarity1
Binding sitei548NADP; via carbonyl oxygenBy similarity1
Binding sitei548SubstrateBy similarity1
Binding sitei616SubstrateBy similarity1
Binding sitei635SubstrateBy similarity1
Active sitei640Proton acceptor; for G6PD activityBy similarity1
Binding sitei745SubstrateBy similarity1
Binding sitei779SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi345 – 352NADPBy similarity8

GO - Molecular functioni

  • 6-phosphogluconolactonase activity Source: GeneDB
  • glucose-6-phosphate dehydrogenase activity Source: GeneDB
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose 6-phosphate metabolic process Source: GeneDB
  • glucose metabolic process Source: UniProtKB-KW
  • pentose-phosphate shunt Source: GeneDB

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408
UPA00115; UER00409

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase1 Publication
Short name:
PfGluPho1 Publication
Including the following 2 domains:
6-phosphogluconolactonase1 Publication (EC:3.1.1.311 Publication)
Short name:
6PGL1 Publication
Glucose-6-phosphate 1-dehydrogenase1 Publication (EC:1.1.1.491 Publication)
Short name:
G6PD1 Publication
Gene namesi
ORF Names:PF14_0511
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_1453800

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004245581 – 910Bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonaseAdd BLAST910

Keywords - PTMi

Glutathionylation

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8IKU0
SMRiQ8IKU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 1706-phosphogluconolactonaseSequence analysisAdd BLAST170
Regioni171 – 276LinkerSequence analysisAdd BLAST106
Regioni277 – 910Glucose-6-phosphate 1-dehydrogenaseSequence analysisAdd BLAST634
Regioni578 – 582Substrate bindingBy similarity5

Sequence similaritiesi

In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.Sequence analysis
In the C-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000282031
InParanoidiQ8IKU0
KOiK00036
OMAiFFIIDER
PhylomeDBiQ8IKU0

Family and domain databases

CDDicd01400 6PGL, 1 hit
HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR005900 6-phosphogluconolactonase_DevB
IPR001282 G6P_DH
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR006148 Glc/Gal-6P_isomerase
IPR036291 NAD(P)-bd_dom_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PF01182 Glucosamine_iso, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF100950 SSF100950, 2 hits
SSF51735 SSF51735, 2 hits

Sequencei

Sequence statusi: Complete.

Q8IKU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYENFVKSA EEINNLHNVN YLETKDLNDF NWKAAYYICK EIYDKQQINK
60 70 80 90 100
DGYVVIGLSG GRTPIDVYKN MCLIKDIKID KSKLIFFIID ERYKSDDHKF
110 120 130 140 150
SNYNNIKFLF HNLNINEKEQ LYKPDTTKSI VDCILDYNDK IKIMIEKYKK
160 170 180 190 200
VDIAILGMGS DFHIASLFPN IFYNIYMNNY QNNYIYNEKT LDFINNDQDN
210 220 230 240 250
DNLKYLKEYV YFTTTNQFDV RKRITVSLNL LANASSKIFL LNSKDKLDLW
260 270 280 290 300
KNMLIKSYIE VNYNLYPATY LIDTSCTNEN VNINNNNNNN NKNKNNYCYS
310 320 330 340 350
NTTVISCGYE NYTKSIEEIY DSKYALSLYS NSLNKEELLT IIIFGCSGDL
360 370 380 390 400
AKKKIYPALF KLFCNNSLPK DLLIIGFART VQDFDTFFDK IVIYLKRCLL
410 420 430 440 450
CYEDWSISKK KDLLNGFKNR CRYFVGNYSS SESFENFNKY LTTIEEEEAK
460 470 480 490 500
KKYYATCYKM NGSDYNISNN VAEDNISIDD ENKTNEYFQM CTPKNCPDNV
510 520 530 540 550
FSSNYNFPYV INRMLYLALP PHIFVSTLKN YKKNCLNSKG TDKILLEKPF
560 570 580 590 600
GNDLDSFKML SKQILENFNE QQIYRIDHYL GKDMVSGLLK LKFTNTFLLS
610 620 630 640 650
LMNRHFIKCI KITLKETKGV YGRGQYFDPY GIIRDVMQNH MLQLLTLITM
660 670 680 690 700
EDPIDLNDES VKNEKIKILK SIPSIKLEDT IIGQYEKAEN FKEDENNDDE
710 720 730 740 750
SKKNHSYHDD PHIDKNSITP TFCTCILYIN SINWYGVPII FKSGKGLNKD
760 770 780 790 800
ICEIRIQFHN IMGSSDENMN NNEFVIILQP VEAIYLKMMI KKTGCEEMEE
810 820 830 840 850
VQLNLTVNEK NKKINVPEAY ETLLLECFKG HKKKFISDEE LYESWRIFTP
860 870 880 890 900
LLKELQEKQV KPLKYSFGSS GPKEVFGLVK KYYNYGKNYT HRPEFVRKSS
910
FYEDDLLDIN
Length:910
Mass (Da):106,987
Last modified:March 1, 2003 - v1
Checksum:i00BF883431E018A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014187 Genomic DNA Translation: AAN37124.1
PIRiS40259
S47533
RefSeqiXP_001348685.1, XM_001348649.2

Genome annotation databases

GeneDBiPF3D7_1453800.1:pep
GeneIDi812093
KEGGipfa:PF14_0511

Similar proteinsi

Entry informationi

Entry nameiGLUPH_PLAF7
AccessioniPrimary (citable) accession number: Q8IKU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health