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Protein

Enolase

Gene

ENO

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  2. Phosphoglycerate kinase (PGK)
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (PF3D7_0626800)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Active sitei218Proton donorBy similarity1
Metal bindingi253MagnesiumBy similarity1
Metal bindingi304MagnesiumBy similarity1
Binding sitei304SubstrateBy similarity1
Metal bindingi331MagnesiumBy similarity1
Binding sitei331SubstrateBy similarity1
Active sitei356Proton acceptorBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • activation of immune response Source: GeneDB
  • autophagosome assembly Source: GeneDB
  • glycolytic process Source: UniProtKB-UniPathway
  • transcription, DNA-templated Source: GeneDB

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.11. 4889.
SABIO-RKiQ8IJN7.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
ORF Names:PF10_0155
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_1015900.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: GeneDB
  • cytoplasm Source: GeneDB
  • cytoskeleton Source: GeneDB
  • food vacuole Source: GeneDB
  • nucleus Source: GeneDB
  • phosphopyruvate hydratase complex Source: InterPro
  • plasma membrane Source: GeneDB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340891 – 446EnolaseAdd BLAST446

PTM databases

SwissPalmiQ8IJN7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi1205736. 9 interactors.
IntActiQ8IJN7. 10 interactors.
MINTiMINT-1545366.

Structurei

3D structure databases

ProteinModelPortaliQ8IJN7.
SMRiQ8IJN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 386Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ8IJN7.
KOiK01689.
OMAiEFMIIPV.
PhylomeDBiQ8IJN7.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IJN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHVITRINA REILDSRGNP TVEVDLETNL GIFRAAVPSG ASTGIYEALE
60 70 80 90 100
LRDNDKSRYL GKGVQKAIKN INEIIAPKLI GMNCTEQKKI DNLMVEELDG
110 120 130 140 150
SKNEWGWSKS KLGANAILAI SMAVCRAGAA ANKVSLYKYL AQLAGKKSDQ
160 170 180 190 200
MVLPVPCLNV INGGSHAGNK LSFQEFMIVP VGAPSFKEAL RYGAEVYHTL
210 220 230 240 250
KSEIKKKYGI DATNVGDEGG FAPNILNANE ALDLLVTAIK SAGYEGKVKI
260 270 280 290 300
AMDVAASEFY NSENKTYDLD FKTPNNDKSL VKTGAQLVDL YIDLVKKYPI
310 320 330 340 350
VSIEDPFDQD DWENYAKLTA AIGKDVQIVG DDLLVTNPTR ITKALEKNAC
360 370 380 390 400
NALLLKVNQI GSITEAIEAC LLSQKNNWGV MVSHRSGETE DVFIADLVVA
410 420 430 440
LRTGQIKTGA PCRSERNAKY NQLLRIEESL GNNAVFAGEK FRLQLN
Length:446
Mass (Da):48,678
Last modified:March 1, 2003 - v1
Checksum:i21ED9FF01BA50DB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014185 Genomic DNA. Translation: AAN35353.1.
RefSeqiXP_001347440.1. XM_001347404.2.

Genome annotation databases

GeneDBiPF3D7_1015900.1:pep.
GeneIDi810313.
KEGGipfa:PF10_0155.

Similar proteinsi

Entry informationi

Entry nameiENO_PLAF7
AccessioniPrimary (citable) accession number: Q8IJN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: September 27, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families