Q8IJN7 (ENO_PLAF7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase
EC=4.2.1.11

Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase

Gene names

Name:	ENO
ORF Names:	PF10_0155

Organism

Plasmodium falciparum (isolate 3D7)

Taxonomic identifier

36329 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Enolase		PRO_0000134089
Regions
	Region	383 – 386	4	Substrate binding By similarity
Sites
	Active site	218	1	Proton donor By similarity
	Active site	356	1	Proton acceptor By similarity
	Metal binding	253	1	Magnesium By similarity
	Metal binding	304	1	Magnesium By similarity
	Metal binding	331	1	Magnesium By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	304	1	Substrate By similarity
	Binding site	331	1	Substrate By similarity
	Binding site	407	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8IJN7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 21ED9FF01BA50DB2	FASTA	446	48,678
	10 20 30 40 50 60 MAHVITRINA REILDSRGNP TVEVDLETNL GIFRAAVPSG ASTGIYEALE LRDNDKSRYL 70 80 90 100 110 120 GKGVQKAIKN INEIIAPKLI GMNCTEQKKI DNLMVEELDG SKNEWGWSKS KLGANAILAI 130 140 150 160 170 180 SMAVCRAGAA ANKVSLYKYL AQLAGKKSDQ MVLPVPCLNV INGGSHAGNK LSFQEFMIVP 190 200 210 220 230 240 VGAPSFKEAL RYGAEVYHTL KSEIKKKYGI DATNVGDEGG FAPNILNANE ALDLLVTAIK 250 260 270 280 290 300 SAGYEGKVKI AMDVAASEFY NSENKTYDLD FKTPNNDKSL VKTGAQLVDL YIDLVKKYPI 310 320 330 340 350 360 VSIEDPFDQD DWENYAKLTA AIGKDVQIVG DDLLVTNPTR ITKALEKNAC NALLLKVNQI 370 380 390 400 410 420 GSITEAIEAC LLSQKNNWGV MVSHRSGETE DVFIADLVVA LRTGQIKTGA PCRSERNAKY 430 440 NQLLRIEESL GNNAVFAGEK FRLQLN

References

[1]

"Genome sequence of the human malaria parasite Plasmodium falciparum." Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D., Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S. , Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A., Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I., Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J., Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G. Nature 419:498-511(2002) [PubMed: 12368864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014185 Genomic DNA. Translation: AAN35353.1.
RefSeq	XP_001347440.1. XM_001347404.2.
3D structure databases
ProteinModelPortal	Q8IJN7.
SMR	Q8IJN7. Positions 5-442.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IJN7. 8 interactions.
MINT	MINT-1545366.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	PF10_0155:mRNA; PF10_0155:pep; PF10_0155.
GeneID	810313.
GenomeReviews	Gene locus ENO in contig AE014185_GR.
KEGG	pfa:PF10_0155.
NMPDR	fig|36329.1.peg.616.
Organism-specific databases
EuPathDB	EupathDB:PF10_0155.
Phylogenomic databases
GeneTree	EPrGT00050000006145.
HOGENOM	HBG726599.
OMA	GELYKNF.
ProtClustDB	PTZ00081.
Enzyme and pathway databases
BRENDA	4.2.1.11. 4889.
Family and domain databases
InterPro	IPR000941. Enolase. IPR020810. Enolase_C. IPR020809. Enolase_CS. IPR020811. Enolase_N. [Graphical view]
KO	K01689.
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
TIGRFAMs	TIGR01060. Eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENO_PLAF7

Accession

Primary (citable) accession number: Q8IJN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)