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Protein

Thioredoxin-T

Gene

TrxT

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probably participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Its tissue specificity suggests a regulatory role in the germline.

Miscellaneous

The TrxT gene, which encodes an testis specific thioredoxin, is adjacent to the dhd gene and shares some regulatory region with it.

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: GO_Central
  • cellular response to DNA damage stimulus Source: FlyBase
  • cellular response to oxidative stress Source: GO_Central
  • glycerol ether metabolic process Source: InterPro
  • oxidation-reduction process Source: UniProtKB
  • protein folding Source: FlyBase

Keywordsi

Biological processElectron transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-T
Short name:
ThioredoxinT
Gene namesi
Name:TrxT
ORF Names:CG3315
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0029752. TrxT.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: Specifically associated with the Y chromosome loops.

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: UniProtKB-SubCell
  • Y chromosome Source: UniProtKB

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001200361 – 157Thioredoxin-TAdd BLAST157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 35Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8IFW4.
PRIDEiQ8IFW4.

Expressioni

Tissue specificityi

Testis specific. Not expressed in the embryo. Becomes progressively more strongly expressed during larval and pupal development. In testis, it is strongly expressed in young spermatocytes, and postmeiotic spermatid stages, then expression decreases at the nuclear elongation stage. Strongly expressed in the waste bag, in which material no longer needed for the mature sperm is eliminated. Not expressed in the stem cells and spermatogonial cells.1 Publication

Developmental stagei

Not expressed maternally.1 Publication

Gene expression databases

BgeeiFBgn0029752.
ExpressionAtlasiQ8IFW4. differential.
GenevisibleiQ8IFW4. DM.

Interactioni

Protein-protein interaction databases

BioGridi57953. 1 interactor.
IntActiQ8IFW4. 1 interactor.
MINTiMINT-1006107.
STRINGi7227.FBpp0070722.

Structurei

3D structure databases

ProteinModelPortaliQ8IFW4.
SMRiQ8IFW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 107ThioredoxinPROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Belongs to the thioredoxin family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
InParanoidiQ8IFW4.
KOiK03671.
OrthoDBiEOG091G01FG.
PhylomeDBiQ8IFW4.

Family and domain databases

InterProiView protein in InterPro
IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiView protein in Pfam
PF00085. Thioredoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01068. thioredoxin. 1 hit.
PROSITEiView protein in PROSITE
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IFW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYPVRNKDD LDQQLILAED KLVVIDFYAD WCGPCKIIAP KLDELAHEYS
60 70 80 90 100
DRVVVLKVNV DENEDITVEY NVNSMPTFVF IKGGNVLELF VGCNSDKLAK
110 120 130 140 150
LMEKHAGVYT DEAADVKAVH IDGECIVDLT AESSESDNDN NNVNEVSAHD

ENAVLEH
Length:157
Mass (Da):17,499
Last modified:March 1, 2003 - v1
Checksum:i5D8EAB28344BFA6B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47 – 48HE → QQ in AAF46018 (PubMed:10731132).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507731 mRNA. Translation: CAD45644.1.
AE014298 Genomic DNA. Translation: AAF46018.2.
RefSeqiNP_001284881.1. NM_001297952.1.
NP_572212.1. NM_131984.1.
UniGeneiDm.8001.

Genome annotation databases

GeneIDi31443.
KEGGidme:Dmel_CG3315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507731 mRNA. Translation: CAD45644.1.
AE014298 Genomic DNA. Translation: AAF46018.2.
RefSeqiNP_001284881.1. NM_001297952.1.
NP_572212.1. NM_131984.1.
UniGeneiDm.8001.

3D structure databases

ProteinModelPortaliQ8IFW4.
SMRiQ8IFW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57953. 1 interactor.
IntActiQ8IFW4. 1 interactor.
MINTiMINT-1006107.
STRINGi7227.FBpp0070722.

Proteomic databases

PaxDbiQ8IFW4.
PRIDEiQ8IFW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi31443.
KEGGidme:Dmel_CG3315.

Organism-specific databases

CTDi31443.
FlyBaseiFBgn0029752. TrxT.

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
InParanoidiQ8IFW4.
KOiK03671.
OrthoDBiEOG091G01FG.
PhylomeDBiQ8IFW4.

Miscellaneous databases

ChiTaRSiTrxT. fly.
GenomeRNAii31443.
PROiPR:Q8IFW4.

Gene expression databases

BgeeiFBgn0029752.
ExpressionAtlasiQ8IFW4. differential.
GenevisibleiQ8IFW4. DM.

Family and domain databases

InterProiView protein in InterPro
IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiView protein in Pfam
PF00085. Thioredoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01068. thioredoxin. 1 hit.
PROSITEiView protein in PROSITE
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIOT_DROME
AccessioniPrimary (citable) accession number: Q8IFW4
Secondary accession number(s): Q9W4D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: May 10, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.