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Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation1 Publication

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathway:iprotein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, apicoplast (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi158 – 1581Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi164 – 1641Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi179 – 1791Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi183 – 1831Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi186 – 1861Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplastUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:lipAUniRule annotation
ORF Names:MAL13P1.220
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
ProteomesiUP000001450 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_1344600.

Subcellular locationi

  • Plastidapicoplast UniRule annotation

GO - Cellular componenti

  • apicoplast Source: GeneDB
Complete GO annotation...

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323UniRule annotationAdd
BLAST
Chaini24 – 415392Lipoyl synthase, apicoplastPRO_0000398230Add
BLAST

Expressioni

Developmental stagei

Expressed maximally in the early stages and late stages of the parasite erythrocytic development.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8IDQ0.
SMRiQ8IDQ0. Positions 124-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
InParanoidiQ8IDQ0.
KOiK03644.
OMAiPVERYWT.
PhylomeDBiQ8IDQ0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8IDQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFGIPSLFY LYILFSIIMR IKCVITKNLK KTKKRTCSYI PHGNMEKGII
60 70 80 90 100
LNYIEKPNPA YLKRGKNKNK NKNKKGDIYK LRNVEILLYA NRYVHEGNEN
110 120 130 140 150
FSSTTKKLLL TPKVGNKMPE GKKPDWFHVA APTVAKYNKL KDDIKKLNLH
160 170 180 190 200
TVCEEAQCPN IGECWNIGTA TIMLLGDTCT RGCKFCSIKT SSNPLPPDIN
210 220 230 240 250
EPFNTAKAIC EWNIDYVVLT SVDRDDLPDG GASHFAKTVE LVKFSRPDIL
260 270 280 290 300
IECLVSDFQG NIDSVRKLAF SGLDVYAHNI ETVKRLQKYV RDKRANYDQS
310 320 330 340 350
LFVLKTAKEI NPQLYTKTSI MLGLGETKEE VIQTMYDARK NNIDVITFGQ
360 370 380 390 400
YLRPTKNHLS IVQYISPQMF EYYKEEGLKM GFKYIASGPL VRSSYKAGEY
410
FIKNLVNQRN KDKKN
Length:415
Mass (Da):47,593
Last modified:March 1, 2003 - v1
Checksum:iFB6D7CE36D52E2C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844509 Genomic DNA. Translation: CAD52569.1.
RefSeqiXP_001350160.1. XM_001350124.1.

Genome annotation databases

EnsemblProtistsiMAL13P1.220:mRNA; MAL13P1.220:pep; MAL13P1.220.
GeneIDi813778.
KEGGipfa:MAL13P1.220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844509 Genomic DNA. Translation: CAD52569.1.
RefSeqiXP_001350160.1. XM_001350124.1.

3D structure databases

ProteinModelPortaliQ8IDQ0.
SMRiQ8IDQ0. Positions 124-399.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiMAL13P1.220:mRNA; MAL13P1.220:pep; MAL13P1.220.
GeneIDi813778.
KEGGipfa:MAL13P1.220.

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_1344600.

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
InParanoidiQ8IDQ0.
KOiK03644.
OMAiPVERYWT.
PhylomeDBiQ8IDQ0.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
    Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
    , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
    Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  3. "The human malaria parasite Plasmodium falciparum has distinct organelle-specific lipoylation pathways."
    Wrenger C., Mueller S.
    Mol. Microbiol. 53:103-113(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiLIPA_PLAF7
AccessioniPrimary (citable) accession number: Q8IDQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.