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Q8IDQ0 (LIPA_PLAF7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase, apicoplast

EC=2.8.1.8
Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase
Gene names
Name:lipA
ORF Names:MAL13P1.220
OrganismPlasmodium falciparum (isolate 3D7) [Reference proteome]
Taxonomic identifier36329 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Ref.3

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123

Subcellular location

Plastidapicoplast By similarity Ref.3.

Developmental stage

Expressed maximally in the early stages and late stages of the parasite erythrocytic development. Ref.3

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 415392Lipoyl synthase, apicoplast HAMAP-Rule MF_03123
PRO_0000398230

Sites

Metal binding1531Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1581Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1641Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1791Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1831Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8IDQ0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: FB6D7CE36D52E2C1

FASTA41547,593
        10         20         30         40         50         60 
MHFGIPSLFY LYILFSIIMR IKCVITKNLK KTKKRTCSYI PHGNMEKGII LNYIEKPNPA 

        70         80         90        100        110        120 
YLKRGKNKNK NKNKKGDIYK LRNVEILLYA NRYVHEGNEN FSSTTKKLLL TPKVGNKMPE 

       130        140        150        160        170        180 
GKKPDWFHVA APTVAKYNKL KDDIKKLNLH TVCEEAQCPN IGECWNIGTA TIMLLGDTCT 

       190        200        210        220        230        240 
RGCKFCSIKT SSNPLPPDIN EPFNTAKAIC EWNIDYVVLT SVDRDDLPDG GASHFAKTVE 

       250        260        270        280        290        300 
LVKFSRPDIL IECLVSDFQG NIDSVRKLAF SGLDVYAHNI ETVKRLQKYV RDKRANYDQS 

       310        320        330        340        350        360 
LFVLKTAKEI NPQLYTKTSI MLGLGETKEE VIQTMYDARK NNIDVITFGQ YLRPTKNHLS 

       370        380        390        400        410 
IVQYISPQMF EYYKEEGLKM GFKYIASGPL VRSSYKAGEY FIKNLVNQRN KDKKN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z. expand/collapse author list , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate 3D7.
[2]"Genome sequence of the human malaria parasite Plasmodium falciparum."
Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D., Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S. expand/collapse author list , Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A., Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I., Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J., Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.
Nature 419:498-511(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate 3D7.
[3]"The human malaria parasite Plasmodium falciparum has distinct organelle-specific lipoylation pathways."
Wrenger C., Mueller S.
Mol. Microbiol. 53:103-113(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL844509 Genomic DNA. Translation: CAD52569.1.
RefSeqXP_001350160.1. XM_001350124.1.

3D structure databases

ProteinModelPortalQ8IDQ0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5833.MAL13P1.220-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsMAL13P1.220:mRNA; MAL13P1.220:pep; MAL13P1.220.
GeneID813778.
KEGGpfa:MAL13P1.220.

Organism-specific databases

EuPathDBPlasmoDB:PF3D7_1344600.

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235998.
KOK03644.
OMAICEWNID.
PhylomeDBQ8IDQ0.
ProtClustDBPTZ00413.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_PLAF7
AccessionPrimary (citable) accession number: Q8IDQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways