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Protein

Calcium-dependent protein kinase 4

Gene

CPK4

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent kinase that plays a central role in the sexual reproduction and mosquito transmission of P.falciparum. Required for cell cycle progression in the male gametocyte, an essential step in parasite transmission to the mosquito (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Intracellular calcium increase is triggered by xanthurenic acid (XA), a small mosquito molecule that induces the differentiation of specialized transmission stages, the gametocytes, into male and female gametes (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99ATPPROSITE-ProRule annotation1
Active sitei193Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 84ATPPROSITE-ProRule annotation9
Calcium bindingi389 – 4001PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi436 – 4472PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi472 – 4833PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi506 – 5174PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 4 (EC:2.7.11.1)
Gene namesi
Name:CPK4
Synonyms:CDPK4
ORF Names:PF07_0072
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0717500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2169725.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000858422 – 528Calcium-dependent protein kinase 4Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

May be myristoylated; myristoylation may target it to different subcellular compartments.By similarity
Autophosphorylated in vitro.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Expressioni

Developmental stagei

Highly expressed in sexual stages, compared to blood asexual stages.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1209973. 2 interactors.
IntActiQ8IBS5. 2 interactors.
MINTiMINT-1718763.

Chemistry databases

BindingDBiQ8IBS5.

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 44Combined sources4
Helixi60 – 62Combined sources3
Turni67 – 69Combined sources3
Beta strandi70 – 79Combined sources10
Beta strandi82 – 89Combined sources8
Turni90 – 92Combined sources3
Beta strandi95 – 102Combined sources8
Turni103 – 105Combined sources3
Beta strandi107 – 110Combined sources4
Helixi112 – 122Combined sources11
Beta strandi133 – 138Combined sources6
Beta strandi140 – 147Combined sources8
Beta strandi152 – 154Combined sources3
Helixi155 – 161Combined sources7
Helixi167 – 186Combined sources20
Helixi196 – 198Combined sources3
Beta strandi199 – 202Combined sources4
Beta strandi210 – 213Combined sources4
Helixi219 – 221Combined sources3
Beta strandi222 – 224Combined sources3
Helixi234 – 236Combined sources3
Helixi240 – 243Combined sources4
Helixi251 – 265Combined sources15
Helixi275 – 284Combined sources10
Helixi292 – 296Combined sources5
Helixi299 – 308Combined sources10
Helixi313 – 315Combined sources3
Helixi319 – 323Combined sources5
Helixi326 – 332Combined sources7
Helixi348 – 388Combined sources41
Beta strandi392 – 397Combined sources6
Helixi398 – 412Combined sources15
Helixi418 – 432Combined sources15
Beta strandi441 – 444Combined sources4
Helixi445 – 471Combined sources27
Turni472 – 474Combined sources3
Beta strandi477 – 479Combined sources3
Helixi481 – 490Combined sources10
Helixi495 – 505Combined sources11
Beta strandi511 – 513Combined sources3
Helixi515 – 527Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QOXX-ray2.75A10-528[»]
4RGJX-ray2.30A10-528[»]
ProteinModelPortaliQ8IBS5.
SMRiQ8IBS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini376 – 411EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini423 – 458EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini459 – 494EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini493 – 528EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ8IBS5.
KOiK13412.
OMAiNKIERDD.
PhylomeDBiQ8IBS5.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8IBS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQEVSSVNN TKNEHHKTNK KSLKGGNERH EMKESSVGIS KKIVENSFNN
60 70 80 90 100
SKLRPGMFIQ NSNVVFNEQY KGIKILGKGS FGEVILSRDK HTGHEYAIKV
110 120 130 140 150
ISKKHVKRKT DKESLLREVE LLKMLDHINI MKLYEFFEDN NYYYLVSDVY
160 170 180 190 200
TGGELFDEII SRKRFYEIDA ARIIKQILSG ITYMHKNNVV HRDLKPENIL
210 220 230 240 250
LETKNKEDMI IKIIDFGLST HFEYSKKMKD KIGTAYYIAP DVLHGTYDEK
260 270 280 290 300
CDIWSCGVIL YILLSGCPPF NGSNEYDILK KVEAGKYTFD LPQFKKISDK
310 320 330 340 350
AKDLIKKMLM YTSAVRISAR DALEHEWIKM MTSKDNLNID IPSLELSIAN
360 370 380 390 400
IRQFQSTQKL AQAALLYMGS KLTTIDETKE LTKIFKKMDK NGDGQLDRNE
410 420 430 440 450
LIIGYKELLK LKGEDTSDLD NAAIEYEVDQ ILNSIDLDQN GYIEYSEFLT
460 470 480 490 500
VSIDRKLLLS TERLEKAFKL FDKDGSGKIS ANELAQLFGL SDVSSECWKT
510 520
VLKEVDQNND GEIDFKEFRD MLVKLCNY
Length:528
Mass (Da):60,780
Last modified:January 23, 2007 - v3
Checksum:i225E3C1F3DFDA3BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844506 Genomic DNA. Translation: CAD50923.1.
RefSeqiXP_001349078.1. XM_001349042.1.

Genome annotation databases

EnsemblProtistsiCAD50923; CAD50923; PF3D7_0717500.
GeneDBiPF3D7_0717500.1:pep.
GeneIDi2655116.
KEGGipfa:PF07_0072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844506 Genomic DNA. Translation: CAD50923.1.
RefSeqiXP_001349078.1. XM_001349042.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QOXX-ray2.75A10-528[»]
4RGJX-ray2.30A10-528[»]
ProteinModelPortaliQ8IBS5.
SMRiQ8IBS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1209973. 2 interactors.
IntActiQ8IBS5. 2 interactors.
MINTiMINT-1718763.

Chemistry databases

BindingDBiQ8IBS5.
ChEMBLiCHEMBL2169725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiCAD50923; CAD50923; PF3D7_0717500.
GeneDBiPF3D7_0717500.1:pep.
GeneIDi2655116.
KEGGipfa:PF07_0072.

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0717500.

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ8IBS5.
KOiK13412.
OMAiNKIERDD.
PhylomeDBiQ8IBS5.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK4_PLAF7
AccessioniPrimary (citable) accession number: Q8IBS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.