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Protein

Calcium-dependent protein kinase 4

Gene

CPK4

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent kinase that plays a central role in the sexual reproduction and mosquito transmission of P.falciparum. Required for cell cycle progression in the male gametocyte, an essential step in parasite transmission to the mosquito (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Intracellular calcium increase is triggered by xanthurenic acid (XA), a small mosquito molecule that induces the differentiation of specialized transmission stages, the gametocytes, into male and female gametes (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991ATPPROSITE-ProRule annotation
Active sitei193 – 1931Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 849ATPPROSITE-ProRule annotation
Calcium bindingi389 – 400121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi436 – 447122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi472 – 483123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi506 – 517124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 4 (EC:2.7.11.1)
Gene namesi
Name:CPK4
Synonyms:CDPK4
ORF Names:PF07_0072
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0717500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2169725.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 528527Calcium-dependent protein kinase 4PRO_0000085842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Post-translational modificationi

May be myristoylated; myristoylation may target it to different subcellular compartments.By similarity
Autophosphorylated in vitro.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PRIDEiQ8IBS5.

Expressioni

Developmental stagei

Highly expressed in sexual stages, compared to blood asexual stages.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1209973. 2 interactions.
IntActiQ8IBS5. 2 interactions.
MINTiMINT-1718763.

Chemistry

BindingDBiQ8IBS5.

Structurei

Secondary structure

1
528
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi41 – 444Combined sources
Helixi60 – 623Combined sources
Turni67 – 693Combined sources
Beta strandi70 – 7910Combined sources
Beta strandi82 – 898Combined sources
Turni90 – 923Combined sources
Beta strandi95 – 1028Combined sources
Turni103 – 1053Combined sources
Beta strandi107 – 1104Combined sources
Helixi112 – 12211Combined sources
Beta strandi133 – 1386Combined sources
Beta strandi140 – 1478Combined sources
Beta strandi152 – 1543Combined sources
Helixi155 – 1617Combined sources
Helixi167 – 18620Combined sources
Helixi196 – 1983Combined sources
Beta strandi199 – 2024Combined sources
Beta strandi210 – 2134Combined sources
Helixi219 – 2213Combined sources
Beta strandi222 – 2243Combined sources
Helixi234 – 2363Combined sources
Helixi240 – 2434Combined sources
Helixi251 – 26515Combined sources
Helixi275 – 28410Combined sources
Helixi292 – 2965Combined sources
Helixi299 – 30810Combined sources
Helixi313 – 3153Combined sources
Helixi319 – 3235Combined sources
Helixi326 – 3327Combined sources
Helixi348 – 38841Combined sources
Beta strandi392 – 3976Combined sources
Helixi398 – 41215Combined sources
Helixi418 – 43215Combined sources
Beta strandi441 – 4444Combined sources
Helixi445 – 47127Combined sources
Turni472 – 4743Combined sources
Beta strandi477 – 4793Combined sources
Helixi481 – 49010Combined sources
Helixi495 – 50511Combined sources
Beta strandi511 – 5133Combined sources
Helixi515 – 52713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QOXX-ray2.75A10-528[»]
4RGJX-ray2.30A10-528[»]
ProteinModelPortaliQ8IBS5.
SMRiQ8IBS5. Positions 16-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 329259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini376 – 41136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini423 – 45836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini459 – 49436EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini493 – 52836EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ8IBS5.
KOiK13412.
OMAiVCIGPRR.
PhylomeDBiQ8IBS5.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8IBS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQEVSSVNN TKNEHHKTNK KSLKGGNERH EMKESSVGIS KKIVENSFNN
60 70 80 90 100
SKLRPGMFIQ NSNVVFNEQY KGIKILGKGS FGEVILSRDK HTGHEYAIKV
110 120 130 140 150
ISKKHVKRKT DKESLLREVE LLKMLDHINI MKLYEFFEDN NYYYLVSDVY
160 170 180 190 200
TGGELFDEII SRKRFYEIDA ARIIKQILSG ITYMHKNNVV HRDLKPENIL
210 220 230 240 250
LETKNKEDMI IKIIDFGLST HFEYSKKMKD KIGTAYYIAP DVLHGTYDEK
260 270 280 290 300
CDIWSCGVIL YILLSGCPPF NGSNEYDILK KVEAGKYTFD LPQFKKISDK
310 320 330 340 350
AKDLIKKMLM YTSAVRISAR DALEHEWIKM MTSKDNLNID IPSLELSIAN
360 370 380 390 400
IRQFQSTQKL AQAALLYMGS KLTTIDETKE LTKIFKKMDK NGDGQLDRNE
410 420 430 440 450
LIIGYKELLK LKGEDTSDLD NAAIEYEVDQ ILNSIDLDQN GYIEYSEFLT
460 470 480 490 500
VSIDRKLLLS TERLEKAFKL FDKDGSGKIS ANELAQLFGL SDVSSECWKT
510 520
VLKEVDQNND GEIDFKEFRD MLVKLCNY
Length:528
Mass (Da):60,780
Last modified:January 23, 2007 - v3
Checksum:i225E3C1F3DFDA3BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844506 Genomic DNA. Translation: CAD50923.1.
RefSeqiXP_001349078.1. XM_001349042.1.

Genome annotation databases

EnsemblProtistsiPF07_0072:mRNA; PF07_0072:pep; PF07_0072.
GeneDBiPF3D7_0717500.1:pep.
GeneIDi2655116.
KEGGipfa:PF07_0072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844506 Genomic DNA. Translation: CAD50923.1.
RefSeqiXP_001349078.1. XM_001349042.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QOXX-ray2.75A10-528[»]
4RGJX-ray2.30A10-528[»]
ProteinModelPortaliQ8IBS5.
SMRiQ8IBS5. Positions 16-528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1209973. 2 interactions.
IntActiQ8IBS5. 2 interactions.
MINTiMINT-1718763.

Chemistry

BindingDBiQ8IBS5.
ChEMBLiCHEMBL2169725.

Proteomic databases

PRIDEiQ8IBS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiPF07_0072:mRNA; PF07_0072:pep; PF07_0072.
GeneDBiPF3D7_0717500.1:pep.
GeneIDi2655116.
KEGGipfa:PF07_0072.

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0717500.

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ8IBS5.
KOiK13412.
OMAiVCIGPRR.
PhylomeDBiQ8IBS5.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  2. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
    Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
    , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
    Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCDPK4_PLAF7
AccessioniPrimary (citable) accession number: Q8IBS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.