Reviewed,
UniProtKB/Swiss-Prot Q8IA42 (GALT4_DROME)
Last modified
June 16, 2009.
Version 48.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: N-acetylgalactosaminyltransferase 4 EC=2.4.1.- Alternative name(s): Protein-UDP acetylgalactosaminyltransferase 4 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 Short name=pp-GaNTase 4 | ||||
| Gene names |
| ||||
| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||
| Taxonomic identifier | 7227 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 659 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified. Prefers the diglycosylated Muc5AC-3/13 as substrate. |
| Cofactor | Manganese By similarity. Calcium By similarity. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein By similarity. |
| Tissue specificity | Expressed in developing oocytes and egg chambers. Ref.1 |
| Developmental stage | Expressed during embryonic, larval, pupal and adult stages. Weakly expressed during early embryonic stages but displays a dramatic increase at 12-24 hours of embryonic development. Continues to be in adult but displays much lower levels in the female adult as compared with the male. Ref.1 |
| Domain | There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane |
| Ligand | Calcium Lectin Manganese |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oligosaccharide biosynthetic process Ref.1 Inferred from direct assay. Source: UniProtKB |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Ref.1Non-traceable author statement. Source: UniProtKB integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activity Ref.1Inferred from direct assay. Source: UniProtKB sugar bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 659 | 659 | N-acetylgalactosaminyltransferase 4 | PRO_0000059158 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 25 | 25 | Cytoplasmic Potential | ||||||||
| Transmembrane | 26 – 48 | 23 | Signal-anchor for type II membrane protein Potential | ||||||||
| Topological domain | 49 – 659 | 611 | Lumenal Potential | ||||||||
| Domain | 511 – 644 | 134 | Ricin B-type lectin | ||||||||
| Region | 192 – 303 | 112 | Catalytic subdomain A | ||||||||
| Region | 360 – 422 | 63 | Catalytic subdomain B | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 172 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 194 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 544 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 580 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 647 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 524 ↔ 541 | By similarity | |||||||||
| Disulfide bond | 571 ↔ 588 | By similarity | |||||||||
| Disulfide bond | 615 ↔ 632 | By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Functional characterization and expression analysis of members of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family from Drosophila melanogaster." Ten Hagen K.G., Tran D.T., Gerken T.A., Stein D.S., Zhang Z. J. Biol. Chem. 278:35039-35048(2003) [PubMed: 12829714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S. Tissue: Embryo. |
| [2] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [3] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. |
| [4] | "Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. One subfamily composed of l(2)35Aa is essential in Drosophila." Schwientek T., Bennett E.P., Flores C., Thacker J., Hollmann M., Reis C.A., Behrens J., Mandel U., Keck B., Schaefer M.A., Haselmann K., Zubarev R., Roepstorff P., Burchell J.M., Taylor-Papadimitriou J., Hollingsworth M.A., Clausen H. J. Biol. Chem. 277:22623-22638(2002) [PubMed: 11925450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 16-659. |
Cross-references
Sequence databases | |
|---|---|
| AY268065 mRNA. Translation: AAQ56701.1. AE014134 Genomic DNA. Translation: AAN10370.1. AF324752 mRNA. Translation: AAN75751.1. | |
| RefSeq | NP_722910.1. |
| UniGene | Dm.14883 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27. |
Genome annotation databases | |
| Ensembl | FBgn0051956. Drosophila melanogaster. [Contig view] |
| GeneID | 261610. |
| KEGG | dme:Dmel_CG31956. |
| NMPDR | fig|7227.3.peg.595. |
Organism-specific databases | |
| FlyBase | FBgn0051956. pgant4. |
Phylogenomic databases | |
| HOGENOM | Q8IA42. |
| OMA | Q8IA42. NISVIFI. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.41. 48. |
Gene expression databases | |
| ArrayExpress | Q8IA42. |
| GermOnline | CG31956. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view] |
| Pfam | PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view] |
| SMART | SM00458. RICIN. 1 hit. [Graphical view] |
| PROSITE | PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 843710. |
Entry information
| Entry name | GALT4_DROME | ||||||||
| Accession | Primary (citable) accession number: Q8IA42 Secondary accession number(s): Q8IQ11 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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