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Q8I7C3

- LASP1_DROME

UniProt

Q8I7C3 - LASP1_DROME

Protein

LIM and SH3 domain protein Lasp

Gene

Lasp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. actin binding Source: FlyBase
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. pole cell formation Source: FlyBase
    2. positive regulation of pole plasm oskar mRNA localization Source: FlyBase
    3. spermatogenesis Source: FlyBase
    4. sperm individualization Source: FlyBase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SignaLinkiQ8I7C3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LIM and SH3 domain protein Lasp
    Gene namesi
    Name:LaspImported
    ORF Names:CG3849
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0063485. Lasp.

    Subcellular locationi

    GO - Cellular componenti

    1. focal adhesion Source: FlyBase
    2. sperm individualization complex Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657LIM and SH3 domain protein LaspPRO_0000075766Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei505 – 5051Phosphoserine1 Publication
    Modified residuei530 – 5301Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8I7C3.

    Expressioni

    Gene expression databases

    BgeeiQ8I7C3.

    Interactioni

    Subunit structurei

    Interacts with osk.

    Protein-protein interaction databases

    STRINGi7227.FBpp0099495.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8I7C3.
    SMRiQ8I7C3. Positions 1-53, 601-653.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 6361LIM zinc-bindingPROSITE-ProRule annotationAdd
    BLAST
    Repeati64 – 9532Nebulin 1Add
    BLAST
    Repeati96 – 13035Nebulin 2Add
    BLAST
    Domaini596 – 65762SH3PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi178 – 594417Gln-richAdd
    BLAST
    Compositional biasi217 – 2204Poly-Pro

    Sequence similaritiesi

    Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
    Contains 2 nebulin repeats.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain, Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG259964.
    GeneTreeiENSGT00730000112971.
    InParanoidiQ8I7C3.
    OMAiQNTKHIS.
    OrthoDBiEOG771280.
    PhylomeDBiQ8I7C3.

    Family and domain databases

    Gene3Di2.10.110.10. 1 hit.
    InterProiIPR000900. Nebulin_repeat.
    IPR001452. SH3_domain.
    IPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 1 hit.
    PF00880. Nebulin. 2 hits.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00132. LIM. 1 hit.
    SM00227. NEBU. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    PS51216. NEBULIN. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A1 Publication (identifier: Q8I7C3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNKTCARCQK VVYPIEELKC LDKTWHKTCF KCTECGMTLN MKTYKGYNKM    50
    PYCEAHIPKA KATAIADTPE LKRIAENTKI QSNVKYHADF EKAKGKFTQV 100
    ADDPETLRIK QNTKHISNVA YHGDLEKKAA MEKQRGSAEV SDSSNESEYF 150
    SEQLAAEQFS QYAPTASPIP PAATTLHQQQ QQLQHQQQQQ YQQHQQQLQQ 200
    QQHQHQHYLQ QQQQTLPPPP IQHQQYNTAA ITPTYQQLQQ QQQQQQQQRA 250
    QQQQLHDPYA HYQQPQALRQ QQQQQQQQQQ QLLQQQAIKQ ASHLYPTATS 300
    QQQQMPPPQS PANPQQQALN SYNEMRSAIL QNSHHPSGNS VDQYDQPQQQ 350
    QHQPQQQSTN PTLVAAQQQQ SHHSLLNNNA SNGGISHSHH SNINNNGHGS 400
    QNQMLPPQMR RSAASVVAYD GNSKQQVAAG PGAAQNHLQQ LYASPNYAAV 450
    TPSENSINVK QHASNGHMPN QQQQHVAGGS NIGKIADYDP LTDGPRVVPN 500
    AGRSSTTLVY SSEPRGNQGG NSVYPKRIGS VSDIDPANGI YGSLTAAEQA 550
    HQQQKHQQYY QQVQMMQQQE HPPQQQQMRQ QPSYSSLQEK QSRQSTAMRV 600
    YRAIYDYEAQ DVDEVSFREG DVIFEVESID SGWMTGRVER TGKTGMLPAN 650
    YVEQAVI 657
    Length:657
    Mass (Da):74,247
    Last modified:January 4, 2005 - v2
    Checksum:i9383E1DEFBF6B529
    GO
    Isoform B1 Publication (identifier: Q8I7C3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         145-145: N → T
         146-298: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:504
    Mass (Da):55,985
    Checksum:iED4F389A19BE8AE0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti190 – 1901Q → QQQQ1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei145 – 1451N → T in isoform B. 2 PublicationsVSP_051636
    Alternative sequencei146 – 298153Missing in isoform B. 2 PublicationsVSP_051637Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ294538 mRNA. Translation: CAC82378.1.
    AE014296 Genomic DNA. Translation: AAF49426.3.
    AE014296 Genomic DNA. Translation: AAN11739.2.
    BT003801 mRNA. Translation: AAO41484.1.
    RefSeqiNP_648912.2. NM_140655.3. [Q8I7C3-2]
    NP_730192.2. NM_168681.4. [Q8I7C3-1]
    UniGeneiDm.15215.

    Genome annotation databases

    EnsemblMetazoaiFBtr0100145; FBpp0099495; FBgn0063485. [Q8I7C3-1]
    GeneIDi39864.
    KEGGidme:Dmel_CG3849.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ294538 mRNA. Translation: CAC82378.1 .
    AE014296 Genomic DNA. Translation: AAF49426.3 .
    AE014296 Genomic DNA. Translation: AAN11739.2 .
    BT003801 mRNA. Translation: AAO41484.1 .
    RefSeqi NP_648912.2. NM_140655.3. [Q8I7C3-2 ]
    NP_730192.2. NM_168681.4. [Q8I7C3-1 ]
    UniGenei Dm.15215.

    3D structure databases

    ProteinModelPortali Q8I7C3.
    SMRi Q8I7C3. Positions 1-53, 601-653.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0099495.

    Proteomic databases

    PaxDbi Q8I7C3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0100145 ; FBpp0099495 ; FBgn0063485 . [Q8I7C3-1 ]
    GeneIDi 39864.
    KEGGi dme:Dmel_CG3849.

    Organism-specific databases

    CTDi 39864.
    FlyBasei FBgn0063485. Lasp.

    Phylogenomic databases

    eggNOGi NOG259964.
    GeneTreei ENSGT00730000112971.
    InParanoidi Q8I7C3.
    OMAi QNTKHIS.
    OrthoDBi EOG771280.
    PhylomeDBi Q8I7C3.

    Enzyme and pathway databases

    SignaLinki Q8I7C3.

    Miscellaneous databases

    GenomeRNAii 39864.
    NextBioi 815773.
    PROi Q8I7C3.

    Gene expression databases

    Bgeei Q8I7C3.

    Family and domain databases

    Gene3Di 2.10.110.10. 1 hit.
    InterProi IPR000900. Nebulin_repeat.
    IPR001452. SH3_domain.
    IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 1 hit.
    PF00880. Nebulin. 2 hits.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00132. LIM. 1 hit.
    SM00227. NEBU. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    PS51216. NEBULIN. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lasp interacts with Osk."
      Jenny A.P., van Berkel W., Filardo P., Ephrussi A.
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Tissue: OvaryImported.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Testis.
    5. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiLASP1_DROME
    AccessioniPrimary (citable) accession number: Q8I7C3
    Secondary accession number(s): Q59E31, Q9VV91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3