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Q8I7C3 (LASP1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LIM and SH3 domain protein Lasp
Gene names
Name:Lasp
ORF Names:CG3849
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with osk.

Sequence similarities

Contains 1 LIM zinc-binding domain.

Contains 2 nebulin repeats.

Contains 1 SH3 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A Ref.5 (identifier: Q8I7C3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B Ref.2 (identifier: Q8I7C3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     145-145: N → T
     146-298: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657LIM and SH3 domain protein Lasp
PRO_0000075766

Regions

Domain3 – 6361LIM zinc-binding
Repeat64 – 9532Nebulin 1
Repeat96 – 13035Nebulin 2
Domain596 – 65762SH3
Compositional bias178 – 594417Gln-rich
Compositional bias217 – 2204Poly-Pro

Amino acid modifications

Modified residue5051Phosphoserine Ref.5
Modified residue5301Phosphoserine Ref.5 Ref.6

Natural variations

Alternative sequence1451N → T in isoform B. Ref.2
VSP_051636
Alternative sequence146 – 298153Missing in isoform B. Ref.2
VSP_051637

Experimental info

Sequence conflict1901Q → QQQQ Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 9383E1DEFBF6B529

FASTA65774,247
        10         20         30         40         50         60 
MNKTCARCQK VVYPIEELKC LDKTWHKTCF KCTECGMTLN MKTYKGYNKM PYCEAHIPKA 

        70         80         90        100        110        120 
KATAIADTPE LKRIAENTKI QSNVKYHADF EKAKGKFTQV ADDPETLRIK QNTKHISNVA 

       130        140        150        160        170        180 
YHGDLEKKAA MEKQRGSAEV SDSSNESEYF SEQLAAEQFS QYAPTASPIP PAATTLHQQQ 

       190        200        210        220        230        240 
QQLQHQQQQQ YQQHQQQLQQ QQHQHQHYLQ QQQQTLPPPP IQHQQYNTAA ITPTYQQLQQ 

       250        260        270        280        290        300 
QQQQQQQQRA QQQQLHDPYA HYQQPQALRQ QQQQQQQQQQ QLLQQQAIKQ ASHLYPTATS 

       310        320        330        340        350        360 
QQQQMPPPQS PANPQQQALN SYNEMRSAIL QNSHHPSGNS VDQYDQPQQQ QHQPQQQSTN 

       370        380        390        400        410        420 
PTLVAAQQQQ SHHSLLNNNA SNGGISHSHH SNINNNGHGS QNQMLPPQMR RSAASVVAYD 

       430        440        450        460        470        480 
GNSKQQVAAG PGAAQNHLQQ LYASPNYAAV TPSENSINVK QHASNGHMPN QQQQHVAGGS 

       490        500        510        520        530        540 
NIGKIADYDP LTDGPRVVPN AGRSSTTLVY SSEPRGNQGG NSVYPKRIGS VSDIDPANGI 

       550        560        570        580        590        600 
YGSLTAAEQA HQQQKHQQYY QQVQMMQQQE HPPQQQQMRQ QPSYSSLQEK QSRQSTAMRV 

       610        620        630        640        650 
YRAIYDYEAQ DVDEVSFREG DVIFEVESID SGWMTGRVER TGKTGMLPAN YVEQAVI 

« Hide

Isoform B [UniParc].

Checksum: ED4F389A19BE8AE0
Show »

FASTA50455,985

References

« Hide 'large scale' references
[1]"Lasp interacts with Osk."
Jenny A.P., van Berkel W., Filardo P., Ephrussi A.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Tissue: Ovary.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Testis.
[5]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ294538 mRNA. Translation: CAC82378.1.
AE014296 Genomic DNA. Translation: AAF49426.3.
AE014296 Genomic DNA. Translation: AAN11739.2.
BT003801 mRNA. Translation: AAO41484.1.
RefSeqNP_648912.2. NM_140655.3. [Q8I7C3-2]
NP_730192.2. NM_168681.4. [Q8I7C3-1]
UniGeneDm.15215.

3D structure databases

ProteinModelPortalQ8I7C3.
SMRQ8I7C3. Positions 1-53, 601-653.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7227.FBpp0099495.

Proteomic databases

PaxDbQ8I7C3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0100145; FBpp0099495; FBgn0063485. [Q8I7C3-1]
GeneID39864.
KEGGdme:Dmel_CG3849.

Organism-specific databases

CTD39864.
FlyBaseFBgn0063485. Lasp.

Phylogenomic databases

eggNOGNOG259964.
GeneTreeENSGT00730000112971.
InParanoidQ8I7C3.
OMAQNTKHIS.
OrthoDBEOG771280.
PhylomeDBQ8I7C3.

Enzyme and pathway databases

SignaLinkQ8I7C3.

Gene expression databases

BgeeQ8I7C3.

Family and domain databases

Gene3D2.10.110.10. 1 hit.
InterProIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi39864.
NextBio815773.
PROQ8I7C3.

Entry information

Entry nameLASP1_DROME
AccessionPrimary (citable) accession number: Q8I7C3
Secondary accession number(s): Q59E31, Q9VV91
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase