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Q8I7C3

- LASP1_DROME

UniProt

Q8I7C3 - LASP1_DROME

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Protein

LIM and SH3 domain protein Lasp

Gene

Lasp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. actin binding Source: FlyBase
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. pole cell formation Source: FlyBase
  2. positive regulation of pole plasm oskar mRNA localization Source: FlyBase
  3. spermatogenesis Source: FlyBase
  4. sperm individualization Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ8I7C3.

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and SH3 domain protein Lasp
Gene namesi
Name:LaspImported
ORF Names:CG3849
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0063485. Lasp.

Subcellular locationi

GO - Cellular componenti

  1. focal adhesion Source: FlyBase
  2. sperm individualization complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657LIM and SH3 domain protein LaspPRO_0000075766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei505 – 5051Phosphoserine1 Publication
Modified residuei530 – 5301Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8I7C3.

Expressioni

Gene expression databases

BgeeiQ8I7C3.

Interactioni

Subunit structurei

Interacts with osk.

Protein-protein interaction databases

STRINGi7227.FBpp0099495.

Structurei

3D structure databases

ProteinModelPortaliQ8I7C3.
SMRiQ8I7C3. Positions 1-30, 601-653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 6361LIM zinc-bindingPROSITE-ProRule annotationAdd
BLAST
Repeati64 – 9532Nebulin 1Add
BLAST
Repeati96 – 13035Nebulin 2Add
BLAST
Domaini596 – 65762SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi178 – 594417Gln-richAdd
BLAST
Compositional biasi217 – 2204Poly-Pro

Sequence similaritiesi

Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
Contains 2 nebulin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG259964.
GeneTreeiENSGT00730000112971.
InParanoidiQ8I7C3.
OMAiQNTKHIS.
OrthoDBiEOG771280.
PhylomeDBiQ8I7C3.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A1 Publication (identifier: Q8I7C3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKTCARCQK VVYPIEELKC LDKTWHKTCF KCTECGMTLN MKTYKGYNKM
60 70 80 90 100
PYCEAHIPKA KATAIADTPE LKRIAENTKI QSNVKYHADF EKAKGKFTQV
110 120 130 140 150
ADDPETLRIK QNTKHISNVA YHGDLEKKAA MEKQRGSAEV SDSSNESEYF
160 170 180 190 200
SEQLAAEQFS QYAPTASPIP PAATTLHQQQ QQLQHQQQQQ YQQHQQQLQQ
210 220 230 240 250
QQHQHQHYLQ QQQQTLPPPP IQHQQYNTAA ITPTYQQLQQ QQQQQQQQRA
260 270 280 290 300
QQQQLHDPYA HYQQPQALRQ QQQQQQQQQQ QLLQQQAIKQ ASHLYPTATS
310 320 330 340 350
QQQQMPPPQS PANPQQQALN SYNEMRSAIL QNSHHPSGNS VDQYDQPQQQ
360 370 380 390 400
QHQPQQQSTN PTLVAAQQQQ SHHSLLNNNA SNGGISHSHH SNINNNGHGS
410 420 430 440 450
QNQMLPPQMR RSAASVVAYD GNSKQQVAAG PGAAQNHLQQ LYASPNYAAV
460 470 480 490 500
TPSENSINVK QHASNGHMPN QQQQHVAGGS NIGKIADYDP LTDGPRVVPN
510 520 530 540 550
AGRSSTTLVY SSEPRGNQGG NSVYPKRIGS VSDIDPANGI YGSLTAAEQA
560 570 580 590 600
HQQQKHQQYY QQVQMMQQQE HPPQQQQMRQ QPSYSSLQEK QSRQSTAMRV
610 620 630 640 650
YRAIYDYEAQ DVDEVSFREG DVIFEVESID SGWMTGRVER TGKTGMLPAN

YVEQAVI
Length:657
Mass (Da):74,247
Last modified:January 4, 2005 - v2
Checksum:i9383E1DEFBF6B529
GO
Isoform B1 Publication (identifier: Q8I7C3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-145: N → T
     146-298: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:504
Mass (Da):55,985
Checksum:iED4F389A19BE8AE0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901Q → QQQQ1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei145 – 1451N → T in isoform B. 2 PublicationsVSP_051636
Alternative sequencei146 – 298153Missing in isoform B. 2 PublicationsVSP_051637Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294538 mRNA. Translation: CAC82378.1.
AE014296 Genomic DNA. Translation: AAF49426.3.
AE014296 Genomic DNA. Translation: AAN11739.2.
BT003801 mRNA. Translation: AAO41484.1.
RefSeqiNP_648912.2. NM_140655.3. [Q8I7C3-2]
NP_730192.2. NM_168681.4. [Q8I7C3-1]
UniGeneiDm.15215.

Genome annotation databases

EnsemblMetazoaiFBtr0100145; FBpp0099495; FBgn0063485. [Q8I7C3-1]
GeneIDi39864.
KEGGidme:Dmel_CG3849.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294538 mRNA. Translation: CAC82378.1 .
AE014296 Genomic DNA. Translation: AAF49426.3 .
AE014296 Genomic DNA. Translation: AAN11739.2 .
BT003801 mRNA. Translation: AAO41484.1 .
RefSeqi NP_648912.2. NM_140655.3. [Q8I7C3-2 ]
NP_730192.2. NM_168681.4. [Q8I7C3-1 ]
UniGenei Dm.15215.

3D structure databases

ProteinModelPortali Q8I7C3.
SMRi Q8I7C3. Positions 1-30, 601-653.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7227.FBpp0099495.

Proteomic databases

PaxDbi Q8I7C3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0100145 ; FBpp0099495 ; FBgn0063485 . [Q8I7C3-1 ]
GeneIDi 39864.
KEGGi dme:Dmel_CG3849.

Organism-specific databases

CTDi 39864.
FlyBasei FBgn0063485. Lasp.

Phylogenomic databases

eggNOGi NOG259964.
GeneTreei ENSGT00730000112971.
InParanoidi Q8I7C3.
OMAi QNTKHIS.
OrthoDBi EOG771280.
PhylomeDBi Q8I7C3.

Enzyme and pathway databases

SignaLinki Q8I7C3.

Miscellaneous databases

ChiTaRSi Lasp. fly.
GenomeRNAii 39864.
NextBioi 815773.
PROi Q8I7C3.

Gene expression databases

Bgeei Q8I7C3.

Family and domain databases

Gene3Di 2.10.110.10. 1 hit.
InterProi IPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view ]
Pfami PF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Lasp interacts with Osk."
    Jenny A.P., van Berkel W., Filardo P., Ephrussi A.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Tissue: OvaryImported.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Testis.
  5. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
    Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
    Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiLASP1_DROME
AccessioniPrimary (citable) accession number: Q8I7C3
Secondary accession number(s): Q59E31, Q9VV91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 26, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3