Q8I7C3 (LASP1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LIM and SH3 domain protein Lasp

Gene names

Name:	Lasp
ORF Names:	CG3849

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with osk.
Sequence similarities	Contains 1 LIM zinc-binding domain. Contains 2 nebulin repeats. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	LIM domain Repeat SH3 domain
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pole cell formation Inferred from mutant phenotype PubMed 19036801. Source: FlyBase positive regulation of pole plasm oskar mRNA localization Inferred from mutant phenotype PubMed 19036801. Source: FlyBase sperm individualization Inferred from mutant phenotype PubMed 18655828. Source: FlyBase
Cellular_component	focal adhesion Inferred from direct assay PubMed 18655828. Source: FlyBase sperm individualization complex Inferred from direct assay PubMed 18655828. Source: FlyBase
Molecular_function	actin binding Inferred from direct assay PubMed 19036801. Source: FlyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A Ref.5 (identifier: Q8I7C3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B Ref.2 (identifier: Q8I7C3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     145-145: N → T
     146-298: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	LIM and SH3 domain protein Lasp		PRO_0000075766
Regions
	Domain	3 – 63	61	LIM zinc-binding
	Repeat	64 – 95	32	Nebulin 1
	Repeat	96 – 130	35	Nebulin 2
	Domain	596 – 657	62	SH3
	Compositional bias	178 – 594	417	Gln-rich
	Compositional bias	217 – 220	4	Poly-Pro
Amino acid modifications
	Modified residue	505	1	Phosphoserine Ref.5
	Modified residue	530	1	Phosphoserine Ref.5 Ref.6
Natural variations
	Alternative sequence	145	1	N → T in isoform B. Ref.2		VSP_051636
	Alternative sequence	146 – 298	153	Missing in isoform B. Ref.2		VSP_051637
Experimental info
	Sequence conflict	190	1	Q → QQQQ Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 9383E1DEFBF6B529	FASTA	657	74,247
	10 20 30 40 50 60 MNKTCARCQK VVYPIEELKC LDKTWHKTCF KCTECGMTLN MKTYKGYNKM PYCEAHIPKA 70 80 90 100 110 120 KATAIADTPE LKRIAENTKI QSNVKYHADF EKAKGKFTQV ADDPETLRIK QNTKHISNVA 130 140 150 160 170 180 YHGDLEKKAA MEKQRGSAEV SDSSNESEYF SEQLAAEQFS QYAPTASPIP PAATTLHQQQ 190 200 210 220 230 240 QQLQHQQQQQ YQQHQQQLQQ QQHQHQHYLQ QQQQTLPPPP IQHQQYNTAA ITPTYQQLQQ 250 260 270 280 290 300 QQQQQQQQRA QQQQLHDPYA HYQQPQALRQ QQQQQQQQQQ QLLQQQAIKQ ASHLYPTATS 310 320 330 340 350 360 QQQQMPPPQS PANPQQQALN SYNEMRSAIL QNSHHPSGNS VDQYDQPQQQ QHQPQQQSTN 370 380 390 400 410 420 PTLVAAQQQQ SHHSLLNNNA SNGGISHSHH SNINNNGHGS QNQMLPPQMR RSAASVVAYD 430 440 450 460 470 480 GNSKQQVAAG PGAAQNHLQQ LYASPNYAAV TPSENSINVK QHASNGHMPN QQQQHVAGGS 490 500 510 520 530 540 NIGKIADYDP LTDGPRVVPN AGRSSTTLVY SSEPRGNQGG NSVYPKRIGS VSDIDPANGI 550 560 570 580 590 600 YGSLTAAEQA HQQQKHQQYY QQVQMMQQQE HPPQQQQMRQ QPSYSSLQEK QSRQSTAMRV 610 620 630 640 650 YRAIYDYEAQ DVDEVSFREG DVIFEVESID SGWMTGRVER TGKTGMLPAN YVEQAVI
	Isoform B [UniParc]. Checksum: ED4F389A19BE8AE0	FASTA	504	55,985
	10 20 30 40 50 60 MNKTCARCQK VVYPIEELKC LDKTWHKTCF KCTECGMTLN MKTYKGYNKM PYCEAHIPKA 70 80 90 100 110 120 KATAIADTPE LKRIAENTKI QSNVKYHADF EKAKGKFTQV ADDPETLRIK QNTKHISNVA 130 140 150 160 170 180 YHGDLEKKAA MEKQRGSAEV SDSSTTSQQQ QMPPPQSPAN PQQQALNSYN EMRSAILQNS 190 200 210 220 230 240 HHPSGNSVDQ YDQPQQQQHQ PQQQSTNPTL VAAQQQQSHH SLLNNNASNG GISHSHHSNI 250 260 270 280 290 300 NNNGHGSQNQ MLPPQMRRSA ASVVAYDGNS KQQVAAGPGA AQNHLQQLYA SPNYAAVTPS 310 320 330 340 350 360 ENSINVKQHA SNGHMPNQQQ QHVAGGSNIG KIADYDPLTD GPRVVPNAGR SSTTLVYSSE 370 380 390 400 410 420 PRGNQGGNSV YPKRIGSVSD IDPANGIYGS LTAAEQAHQQ QKHQQYYQQV QMMQQQEHPP 430 440 450 460 470 480 QQQQMRQQPS YSSLQEKQSR QSTAMRVYRA IYDYEAQDVD EVSFREGDVI FEVESIDSGW 490 500 MTGRVERTGK TGMLPANYVE QAVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lasp interacts with Osk." Jenny A.P., van Berkel W., Filardo P., Ephrussi A. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Ovary.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Testis.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-530, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ294538 mRNA. Translation: CAC82378.1. AE014296 Genomic DNA. Translation: AAF49426.3. AE014296 Genomic DNA. Translation: AAN11739.2. BT003801 mRNA. Translation: AAO41484.1.
RefSeq	NP_648912.2. NM_140655.3. NP_730192.2. NM_168681.4.
UniGene	Dm.15215.
3D structure databases
ProteinModelPortal	Q8I7C3.
SMR	Q8I7C3. Positions 1-30, 601-653.
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0099495.
Proteomic databases
PaxDb	Q8I7C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0100145; FBpp0099495; FBgn0063485.
GeneID	39864.
KEGG	dme:Dmel_CG3849.
Organism-specific databases
CTD	39864.
FlyBase	FBgn0063485. Lasp.
Phylogenomic databases
eggNOG	NOG259964.
GeneTree	ENSGT00530000062924.
InParanoid	Q8I7C3.
OMA	KIADYDP.
OrthoDB	EOG4N8PMS.
PhylomeDB	Q8I7C3.
Gene expression databases
Bgee	Q8I7C3.
GermOnline	CG3849. Drosophila melanogaster.
Family and domain databases
Gene3D	2.10.110.10. 1 hit.
InterPro	IPR000900. Nebulin_35r-motif. IPR001452. SH3_domain. IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00412. LIM. 1 hit. PF00880. Nebulin. 2 hits. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00132. LIM. 1 hit. SM00227. NEBU. 2 hits. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS00478. LIM_DOMAIN_1. 1 hit. PS50023. LIM_DOMAIN_2. 1 hit. PS51216. NEBULIN. 2 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	39864.
NextBio	815773.

Entry information

Entry name

LASP1_DROME

Accession

Primary (citable) accession number: Q8I7C3
Secondary accession number(s): Q59E31, Q9VV91

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project