Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phenoloxidase 2

Gene

PPO2

Organism
Holotrichia diomphalia (Korean black chafer)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (By similarity). Catalyzes the oxidation of o-diphenols (N-acetyldopamine, 4-methylcatechol and dopamine) (PubMed:12185078, PubMed:9085271, PubMed:16362048). Cannot oxidize monophenols and p-phenols (L-tyrosine, tyramine, gentisic acid and hydroquinone) (PubMed:9085271). Binds to the surface of hemocytes and is involved in hemocyte melanization (By similarity). Activation of the enzyme in response to bacterial lipopolysaccharides (LPS) suggests it may play a role in innate immunity (PubMed:9839951).By similarity4 Publications

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 35 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi209Copper ABy similarity1
Metal bindingi213Copper ABy similarity1
Metal bindingi238Copper ABy similarity1
Metal bindingi365Copper BBy similarity1
Metal bindingi369Copper BBy similarity1
Metal bindingi405Copper BBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processImmunity, Innate immunity, Melanin biosynthesis
LigandCopper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase 2Curated (EC:1.14.18.-3 Publications)
Alternative name(s):
Prophenoloxidase-II1 Publication
Gene namesi
Name:PPO2Curated
Synonyms:proPO-IIImported
OrganismiHolotrichia diomphalia (Korean black chafer)Imported
Taxonomic identifieri33394 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaColeopteraPolyphagaScarabaeiformiaScarabaeidaeMelolonthinaeHolotrichia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00004433141 – 51Removed by PPAF11 PublicationAdd BLAST51
ChainiPRO_000044331552 – 684Phenoloxidase 22 PublicationsAdd BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi91N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi330N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi416N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi487N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi491N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi546N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi581 ↔ 623By similarity
Disulfide bondi583 ↔ 630By similarity

Post-translational modificationi

Precursor cleaved by PPAF1.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Hemocytes.1 Publication

Developmental stagei

Expressed in larvae (at protein level).1 Publication

Interactioni

Subunit structurei

Dimer (PubMed:9085271). Might form a homodimer or a heterodimer with PPO1 (PubMed:9085271). Might interact with PPAF2 (via CLIP domain); the interaction might be required for PPO2 activity (PubMed:16362048).2 Publications

Protein-protein interaction databases

ELMiQ8I6K2.

Structurei

3D structure databases

ProteinModelPortaliQ8I6K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiView protein in InterPro
IPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR037020. Hemocyanin_C_sf.
IPR005204. Hemocyanin_N.
IPR036697. Hemocyanin_N_sf.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiView protein in Pfam
PF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8I6K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTAVLNDL VALYDRPTEP MFRVKAKKSF KVPKEYVTDR FKNVAVEISN
60 70 80 90 100
RFGEDDSETV TIDSVPLPDL ADILTLGREE NFSLFIPKHR NLSAKLINIF
110 120 130 140 150
LQAENPKHLL SIACYAHDRV NPYLFIYALS VALIHRKDTK SLKIPNQIQT
160 170 180 190 200
FPDKYFDSQV FSQGKEEMTV VPQGLRRPIE IPRDYTASDL EEEHRVAYWR
210 220 230 240 250
EDLGINLHHW HWHLVYPTDG GEIVTKKDRR GELFYYSHQQ IVARYNFERF
260 270 280 290 300
CNALKRVERL TDWQGPIKEA YFPKLDSLVA KRAYPARVQD MTMQDLDIPG
310 320 330 340 350
QNIKVDVDDM IRWRDRIYRA IADGFITATN GSKMNLDDVT GIDILGNIME
360 370 380 390 400
SSELSPNRQL YGNLHGFGHL MLSYIHDPRS HHLEPFGVIG DFTTAMRDPI
410 420 430 440 450
FYRWHAFVDD VFQQFNGSLP RYTAEQLDYA GVQITDVNIK TPNAPDNEFR
460 470 480 490 500
TFWQQSDVDM SRGVDFQDPG SVFVRFTHLN HEPFSYNITV NNTGNGVQEG
510 520 530 540 550
TCRIFLAPAT DERGNPWLFN NQRVMFVEMD RFKVTLRQGQ NTITRNSTQS
560 570 580 590 600
SVTIPFERTF RDLDTNRPAE GSEELDIFNF CGCGWPHHLL VPKGTPDGFK
610 620 630 640 650
AQLFVMISNY ADDKVEQDLS GSCNDAESYC GVRGGKYPDK RPMGYPFNRV
660 670 680
ARQGADTLQR FLTGNMIVQN CRIVHSDRTV RPRS
Length:684
Mass (Da):78,820
Last modified:March 1, 2003 - v1
Checksum:i3B9F9A712AA96C6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079664 mRNA. Translation: BAC15602.1.

Similar proteinsi

Entry informationi

Entry nameiPPO2_HOLDI
AccessioniPrimary (citable) accession number: Q8I6K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2018
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The sequence was deposited erroneously as PPO1 (PubMed:12185078).Curated

Keywords - Technical termi

Direct protein sequencing