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Q8I5P7 (SYSC_PLAF7) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine--tRNA ligase, cytoplasmic
EC=6.1.1.11
Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene names
ORF Names:PFL0770w
OrganismPlasmodium falciparum (isolate 3D7)
Taxonomic identifier36329 [NCBI]
Taxonomic lineageEukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length618 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. UniProtKB P34945

Catalytic activity

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). UniProtKB P34945

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). UniProtKB P34945

Pathway

Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. UniProtKB P34945

Subunit structure

Homodimer. The tRNA molecule binds across the dimer By similarity. UniProtKB P34945

Subcellular location

Cytoplasm By similarity UniProtKB P49591.

Domain

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. UniProtKB P34945

Miscellaneous

Possible candidate for an effective malaria vaccine as determined by epitope response in sera. Ref.2

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   Developmental stageMerozoite
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processseryl-tRNA aminoacylation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentcytosol

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Non-traceable author statement Ref.2. Source: UniProtKB

mitochondrion

Non-traceable author statement Ref.2. Source: UniProtKB

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-tRNA ligase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 618618Serine--tRNA ligase, cytoplasmic
PRO_0000370214

Regions

Nucleotide binding448 – 4503ATP By similarity UniProtKB P34945
Nucleotide binding536 – 5394ATP By similarity UniProtKB P34945
Region417 – 4193Serine binding By similarity UniProtKB P34945
Compositional bias40 – 302263Lys-rich

Sites

Binding site4721Serine By similarity UniProtKB P34945
Binding site5701Serine By similarity UniProtKB P34945

Sequences

Sequence LengthMass (Da)Tools
Q8I5P7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E0B85181B060DC76

FASTA61873,272
        10         20         30         40         50         60 
MHLSKIIFLL IILFPYHVNS RKPIKYKNVN NVFIEIYPCK AIKWERKKKK RDLIKNNKLI 

        70         80         90        100        110        120 
HNVDVNYINM ITNDNHISSE KCRDIKKKRK ITSSEYGMSL EFFKKNPKKV VQNLKKRGME 

       130        140        150        160        170        180 
KYLNVIVMLK KLINEKNENE VLRNKLRNRR KLLSDHIKNL IFNSKKYEDI INRDITNDSD 

       190        200        210        220        230        240 
HKTNLIHVDE EKKKTEKKND TQDILKDNQK IRYINKENQA TNNNINNQDN SNDVLKKNKI 

       250        260        270        280        290        300 
QIEEIKKETN QINKDIDHIE MNIINLKKKI EFNLYKLPNI LLNKVPKGKT TEDNKIIKFY 

       310        320        330        340        350        360 
KKENIIQLNN EEYDFIEPHE EIIKKYENNF IFSNISNKIG FGYNILVNDI AKLERALIDF 

       370        380        390        400        410        420 
MINTHVNKFL YTYVKAPEIV TKSALFNTGQ LPKFEEDLFK ITDNYKLLNE DAYLIPTSEV 

       430        440        450        460        470        480 
SLLNLFKNSQ IDYIHLPIKL VSHSSCFRTE KNNTYGKTSK GLLREHIFQK VELINITDKK 

       490        500        510        520        530        540 
TSPFYYKKLI KQSTYILKQL NIPYRLVLLN SIETPYSASI CYDIEAWLPS QQRYVEVSSC 

       550        560        570        580        590        600 
SNCLDFQARR LNLKYKIKDS NNFCHTINGS GLAVGRVLAI ILEQYQIKKK HKNEITKIQV 

       610 
PKVLRKYMNK DIIQVEYN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014188 Genomic DNA. Translation: AAN36243.1.
RefSeqXP_001350563.1. XM_001350527.1.

3D structure databases

HSSPHSSP built from PDB template 1WLE based on UniProtKB Q9N0F3.
ProteinModelPortalQ8I5P7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsPFL0770w:mRNA; PFL0770w:pep; PFL0770w.
GeneID811207.
KEGGpfa:PFL0770w.
NMPDRfig|36329.1.peg.1506.

Organism-specific databases

EuPathDBEupathDB:PFL0770w.

Phylogenomic databases

GeneTreeEPrGT00050000002073.
HOGENOMHBG629391.
ProtClustDBCLSZ2431855.

Family and domain databases

InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-synth_IIa.
[Graphical view]
KOK01875.
PANTHERPTHR11778. tRNA-synt_ser. 1 hit.
PfamPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR00981. TRNASYNTHSER.
TIGRFAMsTIGR00414. SerS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYSC_PLAF7
AccessionPrimary (citable) accession number: Q8I5P7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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