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Protein

Receptor-type guanylate cyclase daf-11

Gene

daf-11

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanylate cyclase involved in the production of the second messenger cGMP (PubMed:24015261). In addition, regulates cGMP levels by controlling the transcription of 3',5'-cyclic phosphodiesterase pde-1 and pde-5 mRNAs (PubMed:19489741, PubMed:24015261). Involved in the olfactory, light and pheromone sensing pathways. Part of the chemosensory mechanism of the ASJ sensory neuron that controls dauer formation and dauer recovery (PubMed:1732156, PubMed:8893028). In ASI and ASJ sensory neurons, controls dauer formation and behavioral response to P.aeruginosa by up-regulating the transcription of daf-7, a member of the TGF-beta family (PubMed:11677050, PubMed:25303524). Required for the chemotaxis responses to non-volatile and volatile attractants mediated by the sensory neurons ASE and AWC respectively (PubMed:7828815, PubMed:10790386). Required in ASJ neurons for phototransduction downstream of G protein coupled-photoreceptor lite-1 (PubMed:20436480). Plays a role in the development of ASJ sensory neuron axons during late larval stages and in the maintenance of normal axon morphology in adults (PubMed:9486798). Required to maintain the expression of putative olfactory receptor str-2 in one of the two AWC neurons in adults (PubMed:10571181). Regulates, via the production of cGMP, lifespan (in some environmental conditions), sensitivity to oxidative stress and entry into quiescence triggered by satiety (PubMed:18316030, PubMed:19489741, PubMed:24015261). In AWB and AWC sensory neurons, mediates the recognition of food oders which subsequently allows for the detection of preferred food sources (PubMed:25009271).13 Publications

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi770Magnesium 1PROSITE-ProRule annotation1
Metal bindingi770Magnesium 2PROSITE-ProRule annotation1
Metal bindingi771Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi814Magnesium 1PROSITE-ProRule annotation1
Metal bindingi814Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: InterPro

GO - Biological processi

  • age-dependent response to oxidative stress Source: UniProtKB
  • cellular response to cGMP Source: UniProtKB
  • cGMP biosynthetic process Source: WormBase
  • chemotaxis Source: UniProtKB
  • chemotaxis to cAMP Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • dauer larval development Source: WormBase
  • detection of chemical stimulus Source: UniProtKB
  • determination of adult lifespan Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • negative regulation of dauer larval development Source: UniProtKB
  • olfactory learning Source: WormBase
  • phototransduction Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: WormBase
  • receptor guanylyl cyclase signaling pathway Source: WormBase
  • regulation of axon extension Source: WormBase
  • regulation of chemotaxis Source: UniProtKB
  • regulation of dauer larval development Source: UniProtKB
  • regulation of gene expression Source: UniProtKB
  • regulation of multicellular organism growth Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to biotin Source: UniProtKB
  • response to hydrogen peroxide Source: UniProtKB
  • response to inorganic substance Source: UniProtKB
  • response to pheromone Source: UniProtKB-KW
  • response to temperature stimulus Source: UniProtKB
  • sensory perception of smell Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis, Chemotaxis, Neurogenesis, Olfaction, Pheromone response, Sensory transduction, Transcription, Transcription regulation
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type guanylate cyclase daf-111 Publication (EC:4.6.1.21 Publication)
Alternative name(s):
Abnormal dauer formation protein 11Imported
Gene namesi
Name:daf-11Imported
ORF Names:B0240.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiB0240.3a ; CE42996 ; WBGene00000907 ; daf-11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 334ExtracellularSequence analysis
Transmembranei335 – 355HelicalSequence analysisAdd BLAST21
Topological domaini356 – 1077CytoplasmicSequence analysisAdd BLAST722

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in AWB and AWC sensory neurons results in a defective preference between different food odors.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi806G → E in m84; constitutive dauer formation with mild defect in dauer recovery. Mild inhibition of chemotaxis responses to some volatile attractants. 3 Publications1
Mutagenesisi866S → F in m87; temperature sensitive constitutive dauer formation with severe defect in dauer recovery. Loss of chemotaxis responses to non-volatile and some volatile attractants. 3 Publications1
Mutagenesisi867G → R in p169; temperature sensitive constitutive dauer formation with severe defect in dauer recovery. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000433297? – 1077Receptor-type guanylate cyclase daf-11Curated
Signal peptidei1 – ?Curated

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi14N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi112N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi149N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi311N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8I4N4

Expressioni

Tissue specificityi

Expressed in sensory neurons including ASI, ASJ, ASK, AWB and AWC. Expressed in ASJ neurons in the dauer stage.1 Publication

Gene expression databases

BgeeiWBGene00000907
ExpressionAtlasiQ8I4N4 baseline

Interactioni

Protein-protein interaction databases

STRINGi6239.B0240.3

Structurei

3D structure databases

ProteinModelPortaliQ8I4N4
SMRiQ8I4N4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 695Protein kinasePROSITE-ProRule annotationAdd BLAST341
Domaini765 – 895Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili983 – 1034Sequence analysisAdd BLAST52

Domaini

The protein kinase domain is predicted to be catalytically inactive.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000022503
InParanoidiQ8I4N4
OMAiDKRTEFY
OrthoDBiEOG091G01TW
PhylomeDBiQ8I4N4

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR001828 ANF_lig-bd_rcpt
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8I4N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPTCSSNFL RFANLTAEMQ SLEINILKGY PYEHPTMIDM VTRSPQNLAQ
60 70 80 90 100
NLVSLLRGFE WGQVGAVLCE ECYEGDELAS EIYFSTIEDI FENNNIALKE
110 120 130 140 150
TVRIGKRENS VNISDAITIF EPSARVILLF LGNKLNDYTE FMTAMSMNNY
160 170 180 190 200
TTEEYTPVIV ISKNSLELTF PWKENDAIAE LFDKAIIVYN NCYDKSKISS
210 220 230 240 250
FLSSYSFSTI EETIISLQMY EGYHLLGYYL YTAITNTTLF NYVQPEKAIS
260 270 280 290 300
SMSIPGPFGE IFINSNGQRI AGYDVLVVDK SLNSNNFIMP LGTISTDKKC
310 320 330 340 350
PDQACLNFVL NSTSSFEPLK DVPLCGFHGE ICDQTGVIIA IAVIMGVLLM
360 370 380 390 400
FIIILTTIRK CCNGSKGRSI SNPWVIPFQD VRFIDLTNTE GSQHMSIQSL
410 420 430 440 450
QRNMEEKQRL QSLARTKHIA TVDQVYVLAD KYVMRDKLRY DKIDINLLYQ
460 470 480 490 500
MKSHLQHDNL NSFVGITIDK ASHMYIIWNQ CFRGSLHDHI FTKERQRGTA
510 520 530 540 550
TRFEGLFLRD ILKGLEYIHA SAIDFHGNLT LHNCMLDSHW IVKLSGFGVN
560 570 580 590 600
RLLVKWKTSG QIFTEDHTPV IKSEELHYFD PAMKKIWKNY ADRNERALIT
610 620 630 640 650
PQFGKKCDMY SFGVILHEII LKKKFVEQLF DSPREEDDSV LIDDENDAIA
660 670 680 690 700
SRFPLPIIIP EGIEMHNDLI KMLENCFGSV RPDIALARKI IDTVLKMSGS
710 720 730 740 750
LVDLMIKNLT AYTQGLNETV KNRTAELEKE QEKGDQLLME LLPKSVANDL
760 770 780 790 800
KNGIAVDPKV YENATILYSD IVGFTSLCSQ SQPMEVVTLL SGMYQRFDLI
810 820 830 840 850
ISQQGGYKME TIGDAYCVAA GLPVVMEKDH VKSICMIALL QRDCLHHFEI
860 870 880 890 900
PHRPGTFLNC RWGFNSGPVF AGVIGQKAPR YACFGEAVIL ASKMESSGVE
910 920 930 940 950
DRIQMTLASQ QLLEENFPQF VCSNRGGRTI EGIGRILTYW LEGVNAGEQV
960 970 980 990 1000
KVVEFQNDLN DELSRIMKKD GELLAAATAL KPKDKMTLAK EKVIAERKNE
1010 1020 1030 1040 1050
EERLQRQQTL QEALEEHEEE IEMNEVLVDE DEGEGKPKEV DLTSIVSTQM
1060 1070
EELEDEPAGR TIGHGRLDSQ ASTIPDN
Length:1,077
Mass (Da):121,866
Last modified:November 4, 2008 - v3
Checksum:i7DDD48C3F8101F37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284605 Genomic DNA Translation: CAD56284.3
RefSeqiNP_505960.3, NM_073559.5
UniGeneiCel.5295

Genome annotation databases

EnsemblMetazoaiB0240.3a; B0240.3a; WBGene00000907
GeneIDi179605
KEGGicel:CELE_B0240.3
UCSCiB0240.3 c. elegans

Similar proteinsi

Entry informationi

Entry nameiDAF11_CAEEL
AccessioniPrimary (citable) accession number: Q8I4N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: November 4, 2008
Last modified: March 28, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health