Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8I1F6

- PPO3_DROER

UniProt

Q8I1F6 - PPO3_DROER

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phenoloxidase 3

Gene

PPO3

Organism
Drosophila erecta (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone (By similarity).By similarity

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi209 – 2091Copper ABy similarity
Metal bindingi213 – 2131Copper ABy similarity
Metal bindingi239 – 2391Copper ABy similarity
Metal bindingi366 – 3661Copper BBy similarity
Metal bindingi370 – 3701Copper BBy similarity
Metal bindingi406 – 4061Copper BBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase 3 (EC:1.14.18.1)
Alternative name(s):
Diphenoloxidase subunit A3
Tyrosinase A3
Gene namesi
Name:PPO3
Synonyms:Dox-A3, proPO59
ORF Names:GG22822
OrganismiDrosophila erecta (Fruit fly)
Taxonomic identifieri7220 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000008711: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0064624. Dere\GG22822.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 4848By similarityPRO_0000035903Add
BLAST
Chaini51 – 683633Phenoloxidase 3PRO_0000035904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi574 ↔ 617By similarity
Disulfide bondi576 ↔ 624By similarity

Post-translational modificationi

Upon activation, a trypsin type protease cleaves prophenol oxidase to yield the active enzyme.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ8I1F6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Sequence Analysis

Phylogenomic databases

KOiK00505.
OrthoDBiEOG7XPZ54.
PhylomeDBiQ8I1F6.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8I1F6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADKKNLLLL FDHPTEPVFM DKGGNGTVFD VPASYVTDRY NKMCKKVQRR
60 70 80 90 100
VSGGFEKNVL VKEIPIPDLS CSMRLGRSEQ FSLFLESHRQ MACHLIDVFI
110 120 130 140 150
KMPTVDELQS VAVYARDRVN PVLFNYALSV AMLHRSDTKD LGLPAFAQIF
160 170 180 190 200
PDRFIDSQML RTMREESFVV ERSAARLPVH SSVKYTASDL DVEHRLWYFR
210 220 230 240 250
EDLGVNLHHW HWHLVYPNTA PDRSIVDKDR RGELFYYMHQ QIIARYNAER
260 270 280 290 300
LCNHMARVQP FNNLEEPIAE GYFPKMDSLV ASRAFPPRFD NTRLSDVDRP
310 320 330 340 350
INQLRVGIDD MKRWRERIYE AIHQGYVLDA NHKKIVLDDV KGIDILGNII
360 370 380 390 400
ESSQLTPNKT LYGDLHNKGH ILIAFSHDPT NKHLEYAGVM GDASTAMRDP
410 420 430 440 450
IFYKWHAFID NLFQEHKRQL SPYTEEDLTF PDVRVQSIQV ESQGQVNRLT
460 470 480 490 500
TFWQESDVDM SRGLDFVPRG HVLARFTHLQ HHPFSYTIEV ENSSEATRYG
510 520 530 540 550
YVRIFLAPKL DDGNATMLLE QQRRMMVELD KFVVTMPPGS HTITRDSTES
560 570 580 590 600
SVTIPFKRTF RNMDNPGEPQ NFLCGCGWPQ HMLIPKGRAE GLSFELFVMV
610 620 630 640 650
SNYEDDKVDQ KPEDCECSIA ASYCGLRDRL YPDRKSMGFP FDRQPRSGSE
660 670 680
LLEKFLTPNM CSIEVIISHE ARTEKIPELP DHS
Length:683
Mass (Da):79,020
Last modified:September 22, 2009 - v2
Checksum:iE8955B2798B6C260
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281L → M in AAO01013. (PubMed:12537575)Curated
Sequence conflicti557 – 5571K → E in AAO01013. (PubMed:12537575)Curated
Sequence conflicti568 – 5692EP → QL in AAO01013. (PubMed:12537575)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY190941 Genomic DNA. Translation: AAO01013.1.
CH954179 Genomic DNA. Translation: EDV56739.1.
RefSeqiXP_001976339.1. XM_001976303.1.

Genome annotation databases

EnsemblMetazoaiFBtr0142876; FBpp0141368; FBgn0064624.
GeneIDi6547312.
KEGGider:Dere_GG22822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY190941 Genomic DNA. Translation: AAO01013.1 .
CH954179 Genomic DNA. Translation: EDV56739.1 .
RefSeqi XP_001976339.1. XM_001976303.1.

3D structure databases

ProteinModelPortali Q8I1F6.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0142876 ; FBpp0141368 ; FBgn0064624 .
GeneIDi 6547312.
KEGGi der:Dere_GG22822.

Organism-specific databases

FlyBasei FBgn0064624. Dere\GG22822.

Phylogenomic databases

KOi K00505.
OrthoDBi EOG7XPZ54.
PhylomeDBi Q8I1F6.

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProi IPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
PANTHERi PTHR11511. PTHR11511. 1 hit.
Pfami PF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view ]
PRINTSi PR00187. HAEMOCYANIN.
SUPFAMi SSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 14021-0224.01.

Entry informationi

Entry nameiPPO3_DROER
AccessioniPrimary (citable) accession number: Q8I1F6
Secondary accession number(s): B3NP06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: September 22, 2009
Last modified: October 29, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3