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Q8I1F6

- PPO3_DROER

UniProt

Q8I1F6 - PPO3_DROER

Protein

Phenoloxidase 3

Gene

PPO3

Organism
Drosophila erecta (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone By similarity.By similarity

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi209 – 2091Copper ABy similarity
    Metal bindingi213 – 2131Copper ABy similarity
    Metal bindingi239 – 2391Copper ABy similarity
    Metal bindingi366 – 3661Copper BBy similarity
    Metal bindingi370 – 3701Copper BBy similarity
    Metal bindingi406 – 4061Copper BBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenoloxidase 3 (EC:1.14.18.1)
    Alternative name(s):
    Diphenoloxidase subunit A3
    Tyrosinase A3
    Gene namesi
    Name:PPO3
    Synonyms:Dox-A3, proPO59
    ORF Names:GG22822
    OrganismiDrosophila erecta (Fruit fly)
    Taxonomic identifieri7220 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000008711: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0064624. Dere\GG22822.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 4848By similarityPRO_0000035903Add
    BLAST
    Chaini51 – 683633Phenoloxidase 3PRO_0000035904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi574 ↔ 617By similarity
    Disulfide bondi576 ↔ 624By similarity

    Post-translational modificationi

    Upon activation, a trypsin type protease cleaves prophenol oxidase to yield the active enzyme.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliQ8I1F6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Sequence Analysis

    Phylogenomic databases

    KOiK00505.
    OrthoDBiEOG7XPZ54.
    PhylomeDBiQ8I1F6.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    1.20.1370.10. 1 hit.
    2.60.40.1520. 1 hit.
    InterProiIPR013788. Hemocyanin/hexamerin.
    IPR000896. Hemocyanin/hexamerin_mid_dom.
    IPR005203. Hemocyanin_C.
    IPR005204. Hemocyanin_N.
    IPR014756. Ig_E-set.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PANTHERiPTHR11511. PTHR11511. 1 hit.
    PfamiPF03723. Hemocyanin_C. 1 hit.
    PF00372. Hemocyanin_M. 1 hit.
    PF03722. Hemocyanin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00187. HAEMOCYANIN.
    SUPFAMiSSF48050. SSF48050. 1 hit.
    SSF48056. SSF48056. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
    PS00210. HEMOCYANIN_2. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8I1F6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADKKNLLLL FDHPTEPVFM DKGGNGTVFD VPASYVTDRY NKMCKKVQRR    50
    VSGGFEKNVL VKEIPIPDLS CSMRLGRSEQ FSLFLESHRQ MACHLIDVFI 100
    KMPTVDELQS VAVYARDRVN PVLFNYALSV AMLHRSDTKD LGLPAFAQIF 150
    PDRFIDSQML RTMREESFVV ERSAARLPVH SSVKYTASDL DVEHRLWYFR 200
    EDLGVNLHHW HWHLVYPNTA PDRSIVDKDR RGELFYYMHQ QIIARYNAER 250
    LCNHMARVQP FNNLEEPIAE GYFPKMDSLV ASRAFPPRFD NTRLSDVDRP 300
    INQLRVGIDD MKRWRERIYE AIHQGYVLDA NHKKIVLDDV KGIDILGNII 350
    ESSQLTPNKT LYGDLHNKGH ILIAFSHDPT NKHLEYAGVM GDASTAMRDP 400
    IFYKWHAFID NLFQEHKRQL SPYTEEDLTF PDVRVQSIQV ESQGQVNRLT 450
    TFWQESDVDM SRGLDFVPRG HVLARFTHLQ HHPFSYTIEV ENSSEATRYG 500
    YVRIFLAPKL DDGNATMLLE QQRRMMVELD KFVVTMPPGS HTITRDSTES 550
    SVTIPFKRTF RNMDNPGEPQ NFLCGCGWPQ HMLIPKGRAE GLSFELFVMV 600
    SNYEDDKVDQ KPEDCECSIA ASYCGLRDRL YPDRKSMGFP FDRQPRSGSE 650
    LLEKFLTPNM CSIEVIISHE ARTEKIPELP DHS 683
    Length:683
    Mass (Da):79,020
    Last modified:September 22, 2009 - v2
    Checksum:iE8955B2798B6C260
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti328 – 3281L → M in AAO01013. (PubMed:12537575)Curated
    Sequence conflicti557 – 5571K → E in AAO01013. (PubMed:12537575)Curated
    Sequence conflicti568 – 5692EP → QL in AAO01013. (PubMed:12537575)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY190941 Genomic DNA. Translation: AAO01013.1.
    CH954179 Genomic DNA. Translation: EDV56739.1.
    RefSeqiXP_001976339.1. XM_001976303.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0142876; FBpp0141368; FBgn0064624.
    GeneIDi6547312.
    KEGGider:Dere_GG22822.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY190941 Genomic DNA. Translation: AAO01013.1 .
    CH954179 Genomic DNA. Translation: EDV56739.1 .
    RefSeqi XP_001976339.1. XM_001976303.1.

    3D structure databases

    ProteinModelPortali Q8I1F6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0142876 ; FBpp0141368 ; FBgn0064624 .
    GeneIDi 6547312.
    KEGGi der:Dere_GG22822.

    Organism-specific databases

    FlyBasei FBgn0064624. Dere\GG22822.

    Phylogenomic databases

    KOi K00505.
    OrthoDBi EOG7XPZ54.
    PhylomeDBi Q8I1F6.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    1.20.1370.10. 1 hit.
    2.60.40.1520. 1 hit.
    InterProi IPR013788. Hemocyanin/hexamerin.
    IPR000896. Hemocyanin/hexamerin_mid_dom.
    IPR005203. Hemocyanin_C.
    IPR005204. Hemocyanin_N.
    IPR014756. Ig_E-set.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    PANTHERi PTHR11511. PTHR11511. 1 hit.
    Pfami PF03723. Hemocyanin_C. 1 hit.
    PF00372. Hemocyanin_M. 1 hit.
    PF03722. Hemocyanin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00187. HAEMOCYANIN.
    SUPFAMi SSF48050. SSF48050. 1 hit.
    SSF48056. SSF48056. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00209. HEMOCYANIN_1. 1 hit.
    PS00210. HEMOCYANIN_2. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 14021-0224.01.

    Entry informationi

    Entry nameiPPO3_DROER
    AccessioniPrimary (citable) accession number: Q8I1F6
    Secondary accession number(s): B3NP06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3