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Protein

Polypeptide N-acetylgalactosaminyltransferase 4

Gene

gly-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei191 – 1911SubstrateBy similarity
Binding sitei216 – 2161SubstrateBy similarity
Metal bindingi239 – 2391ManganeseBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Metal bindingi241 – 2411ManganeseBy similarity
Binding sitei346 – 3461SubstrateBy similarity
Metal bindingi374 – 3741ManganeseBy similarity
Binding sitei377 – 3771SubstrateBy similarity
Binding sitei380 – 3801SubstrateBy similarity
Binding sitei382 – 3821SubstrateBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: WormBase

GO - Biological processi

  1. protein O-linked glycosylation via threonine Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.41)
Short name:
pp-GaNTase 4
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
Gene namesi
Name:gly-4
ORF Names:Y116F11B.12
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY116F11B.12a; CE26046; WBGene00001629; gly-4.
Y116F11B.12b; CE32074; WBGene00001629; gly-4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 3120Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 589558LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Polypeptide N-acetylgalactosaminyltransferase 4PRO_0000059147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 369PROSITE-ProRule annotation
Disulfide bondi360 ↔ 438PROSITE-ProRule annotation
Disulfide bondi471 ↔ 488PROSITE-ProRule annotation
Disulfide bondi514 ↔ 531PROSITE-ProRule annotation
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi553 ↔ 571PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8I136.
PRIDEiQ8I136.

Expressioni

Gene expression databases

ExpressionAtlasiQ8I136. baseline.

Interactioni

Protein-protein interaction databases

BioGridi45263. 2 interactions.
DIPiDIP-26941N.
IntActiQ8I136. 1 interaction.
MINTiMINT-1107053.

Structurei

3D structure databases

ProteinModelPortaliQ8I136.
SMRiQ8I136. Positions 90-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini458 – 589132Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 255106Catalytic subdomain AAdd
BLAST
Regioni315 – 37763Catalytic subdomain BAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 7236Pro-richAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00760000118828.
HOGENOMiHOG000038227.
InParanoidiQ8I136.
KOiK00710.
OMAiHERTEKD.
PhylomeDBiQ8I136.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q8I136-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPRMLKMKT VGTVLAVIWL FGLAFIYVQS TSSSLRPPGR HPPPLPQLDP
60 70 80 90 100
LIPQNPPQND EIRPKKSAPP IPTINLAEDT TIHERTEKDV TWKTFDVEKF
110 120 130 140 150
LNKGKWHQGE DKYKANSFNQ EASDALNPTR KIPDSREPQC RDVDYSKVGM
160 170 180 190 200
QPTTVIITYH NEARSSLLRT VFSVFNQSPE ELLLEIVLVD DNSQDVEIGK
210 220 230 240 250
ELAQIQRITV LRNNQREGLI RSRVKGAQVA RAPVLTFLDS HIECNQKWLE
260 270 280 290 300
PLLARIAENP KAVVAPIIDV INVDNFNYVG ASADLRGGFD WTLVFRWEFM
310 320 330 340 350
NEQLRKERHA HPTAPIRSPT MAGGLFAISK EWFNELGTYD LDMEVWGGEN
360 370 380 390 400
LEMSFRVWQC GGSLEIMPCS RVGHVFRKKH PYTFPGGSGN VFQKNTRRAA
410 420 430 440 450
EVWMDEYKAI YLKNVPSARF VNFGDITDRL AIRDRLQCKS FKWYLENVYP
460 470 480 490 500
QLEIPRKTPG KSFQMKIGNL CLDSMARKES EAPGLFGCHG TGGNQEWVFD
510 520 530 540 550
QLTKTFKNAI SQLCLDFSSN TENKTVTMVK CENLRPDTMV VEKNGWLTQG
560 570 580
GKCLTVNQGS GGDWLIYGAH CELNNGAQRW IFEKLDTYE
Length:589
Mass (Da):67,031
Last modified:August 15, 2004 - v2
Checksum:i108D621D91A329D2
GO
Isoform b (identifier: Q8I136-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-453: E → D
     454-589: Missing.

Note: No experimental confirmation available.

Show »
Length:453
Mass (Da):51,879
Checksum:i572763F5D4D25B53
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei453 – 4531E → D in isoform b. CuratedVSP_011236
Alternative sequencei454 – 589136Missing in isoform b. CuratedVSP_011237Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031834 mRNA. Translation: AAC13670.1.
AL132943 Genomic DNA. Translation: CAB81985.3.
AL132943 Genomic DNA. Translation: CAC14394.1.
PIRiT42244.
RefSeqiNP_001024216.1. NM_001029045.3. [Q8I136-1]
NP_507850.2. NM_075449.7. [Q8I136-2]
UniGeneiCel.19666.

Genome annotation databases

EnsemblMetazoaiY116F11B.12a.1; Y116F11B.12a.1; WBGene00001629. [Q8I136-1]
Y116F11B.12a.2; Y116F11B.12a.2; WBGene00001629. [Q8I136-1]
GeneIDi180302.
KEGGicel:CELE_Y116F11B.12.
UCSCiY116F11B.12b.1. c. elegans. [Q8I136-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031834 mRNA. Translation: AAC13670.1.
AL132943 Genomic DNA. Translation: CAB81985.3.
AL132943 Genomic DNA. Translation: CAC14394.1.
PIRiT42244.
RefSeqiNP_001024216.1. NM_001029045.3. [Q8I136-1]
NP_507850.2. NM_075449.7. [Q8I136-2]
UniGeneiCel.19666.

3D structure databases

ProteinModelPortaliQ8I136.
SMRiQ8I136. Positions 90-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45263. 2 interactions.
DIPiDIP-26941N.
IntActiQ8I136. 1 interaction.
MINTiMINT-1107053.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PaxDbiQ8I136.
PRIDEiQ8I136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY116F11B.12a.1; Y116F11B.12a.1; WBGene00001629. [Q8I136-1]
Y116F11B.12a.2; Y116F11B.12a.2; WBGene00001629. [Q8I136-1]
GeneIDi180302.
KEGGicel:CELE_Y116F11B.12.
UCSCiY116F11B.12b.1. c. elegans. [Q8I136-1]

Organism-specific databases

CTDi180302.
WormBaseiY116F11B.12a; CE26046; WBGene00001629; gly-4.
Y116F11B.12b; CE32074; WBGene00001629; gly-4.

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00760000118828.
HOGENOMiHOG000038227.
InParanoidiQ8I136.
KOiK00710.
OMAiHERTEKD.
PhylomeDBiQ8I136.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi908800.
PROiQ8I136.

Gene expression databases

ExpressionAtlasiQ8I136. baseline.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
    Hagen F.K., Nehrke K.
    J. Biol. Chem. 273:8268-8277(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), ENZYME ACTIVITY.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiGALT4_CAEEL
AccessioniPrimary (citable) accession number: Q8I136
Secondary accession number(s): O61390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2004
Last sequence update: August 15, 2004
Last modified: January 6, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.