Q8I136 (GALT4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 4
Short name=pp-GaNTase 4
EC=2.4.1.41

Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4

Gene names

Name:	gly-4
ORF Names:	Y116F11B.12

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.
Catalytic activity	UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide. Ref.1
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation via threonine Inferred from direct assay Ref.1. Source: WormBase
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	polypeptide N-acetylgalactosaminyltransferase activity Inferred from direct assay Ref.1. Source: WormBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q8I136-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q8I136-2)

The sequence of this isoform differs from the canonical sequence as follows:
     453-453: E → D
     454-589: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Polypeptide N-acetylgalactosaminyltransferase 4		PRO_0000059147
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 31	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 589	558	Lumenal Potential
	Domain	458 – 589	132	Ricin B-type lectin
	Region	150 – 255	106	Catalytic subdomain A
	Region	315 – 377	63	Catalytic subdomain B
	Compositional bias	37 – 72	36	Pro-rich
Amino acid modifications
	Glycosylation	523	1	N-linked (GlcNAc...) Potential
	Disulfide bond	471 ↔ 488		By similarity
	Disulfide bond	514 ↔ 531		By similarity
	Disulfide bond	553 ↔ 571		By similarity
Natural variations
	Alternative sequence	453	1	E → D in isoform b.		VSP_011236
	Alternative sequence	454 – 589	136	Missing in isoform b.		VSP_011237

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 108D621D91A329D2	FASTA	589	67,031
	10 20 30 40 50 60 MLPRMLKMKT VGTVLAVIWL FGLAFIYVQS TSSSLRPPGR HPPPLPQLDP LIPQNPPQND 70 80 90 100 110 120 EIRPKKSAPP IPTINLAEDT TIHERTEKDV TWKTFDVEKF LNKGKWHQGE DKYKANSFNQ 130 140 150 160 170 180 EASDALNPTR KIPDSREPQC RDVDYSKVGM QPTTVIITYH NEARSSLLRT VFSVFNQSPE 190 200 210 220 230 240 ELLLEIVLVD DNSQDVEIGK ELAQIQRITV LRNNQREGLI RSRVKGAQVA RAPVLTFLDS 250 260 270 280 290 300 HIECNQKWLE PLLARIAENP KAVVAPIIDV INVDNFNYVG ASADLRGGFD WTLVFRWEFM 310 320 330 340 350 360 NEQLRKERHA HPTAPIRSPT MAGGLFAISK EWFNELGTYD LDMEVWGGEN LEMSFRVWQC 370 380 390 400 410 420 GGSLEIMPCS RVGHVFRKKH PYTFPGGSGN VFQKNTRRAA EVWMDEYKAI YLKNVPSARF 430 440 450 460 470 480 VNFGDITDRL AIRDRLQCKS FKWYLENVYP QLEIPRKTPG KSFQMKIGNL CLDSMARKES 490 500 510 520 530 540 EAPGLFGCHG TGGNQEWVFD QLTKTFKNAI SQLCLDFSSN TENKTVTMVK CENLRPDTMV 550 560 570 580 VEKNGWLTQG GKCLTVNQGS GGDWLIYGAH CELNNGAQRW IFEKLDTYE
	Isoform b [UniParc]. Checksum: 572763F5D4D25B53	FASTA	453	51,879
	10 20 30 40 50 60 MLPRMLKMKT VGTVLAVIWL FGLAFIYVQS TSSSLRPPGR HPPPLPQLDP LIPQNPPQND 70 80 90 100 110 120 EIRPKKSAPP IPTINLAEDT TIHERTEKDV TWKTFDVEKF LNKGKWHQGE DKYKANSFNQ 130 140 150 160 170 180 EASDALNPTR KIPDSREPQC RDVDYSKVGM QPTTVIITYH NEARSSLLRT VFSVFNQSPE 190 200 210 220 230 240 ELLLEIVLVD DNSQDVEIGK ELAQIQRITV LRNNQREGLI RSRVKGAQVA RAPVLTFLDS 250 260 270 280 290 300 HIECNQKWLE PLLARIAENP KAVVAPIIDV INVDNFNYVG ASADLRGGFD WTLVFRWEFM 310 320 330 340 350 360 NEQLRKERHA HPTAPIRSPT MAGGLFAISK EWFNELGTYD LDMEVWGGEN LEMSFRVWQC 370 380 390 400 410 420 GGSLEIMPCS RVGHVFRKKH PYTFPGGSGN VFQKNTRRAA EVWMDEYKAI YLKNVPSARF 430 440 450 VNFGDITDRL AIRDRLQCKS FKWYLENVYP QLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans." Hagen F.K., Nehrke K. J. Biol. Chem. 273:8268-8277(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), ENZYME ACTIVITY. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031834 mRNA. Translation: AAC13670.1. AL132943 Genomic DNA. Translation: CAB81985.3. AL132943 Genomic DNA. Translation: CAC14394.1.
PIR	T42244.
RefSeq	NP_001024216.1. NM_001029045.3. NP_507850.2. NM_075449.7.
UniGene	Cel.19666.
3D structure databases
ProteinModelPortal	Q8I136.
SMR	Q8I136. Positions 90-587.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26941N.
IntAct	Q8I136. 1 interaction.
MINT	MINT-1107053.
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Proteomic databases
PaxDb	Q8I136.
PRIDE	Q8I136.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y116F11B.12a.1; Y116F11B.12a.1; Y116F11B.12. Y116F11B.12a.2; Y116F11B.12a.2; Y116F11B.12.
GeneID	180302.
KEGG	cel:CELE_Y116F11B.12.
UCSC	Y116F11B.12b.1. c. elegans.
Organism-specific databases
CTD	180302.
WormBase	Y116F11B.12a; CE26046; WBGene00001629; gly-4. Y116F11B.12b; CE32074; WBGene00001629; gly-4.
Phylogenomic databases
eggNOG	NOG239675.
GeneTree	ENSGT00700000104275.
HOGENOM	HOG000038227.
InParanoid	Q8I136.
KO	K00710.
OMA	IYECHNA.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q8I136.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
SUPFAM	SSF50370. RicinB_like. 1 hit.
PROSITE	PS50231. RICIN_B_LECTIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	908800.

Entry information

Entry name

GALT4_CAEEL

Accession

Primary (citable) accession number: Q8I136
Secondary accession number(s): O61390

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project