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Q8I136

- GALT4_CAEEL

UniProt

Q8I136 - GALT4_CAEEL

Protein

Polypeptide N-acetylgalactosaminyltransferase 4

Gene

gly-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei191 – 1911SubstrateBy similarity
    Binding sitei216 – 2161SubstrateBy similarity
    Metal bindingi239 – 2391ManganeseBy similarity
    Binding sitei240 – 2401SubstrateBy similarity
    Metal bindingi241 – 2411ManganeseBy similarity
    Binding sitei346 – 3461SubstrateBy similarity
    Metal bindingi374 – 3741ManganeseBy similarity
    Binding sitei377 – 3771SubstrateBy similarity
    Binding sitei380 – 3801SubstrateBy similarity
    Binding sitei382 – 3821SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: WormBase

    GO - Biological processi

    1. protein O-linked glycosylation via threonine Source: WormBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.41)
    Short name:
    pp-GaNTase 4
    Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 4
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
    Gene namesi
    Name:gly-4
    ORF Names:Y116F11B.12
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiY116F11B.12a; CE26046; WBGene00001629; gly-4.
    Y116F11B.12b; CE32074; WBGene00001629; gly-4.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 589589Polypeptide N-acetylgalactosaminyltransferase 4PRO_0000059147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 369PROSITE-ProRule annotation
    Disulfide bondi360 ↔ 438PROSITE-ProRule annotation
    Disulfide bondi471 ↔ 488PROSITE-ProRule annotation
    Disulfide bondi514 ↔ 531PROSITE-ProRule annotation
    Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi553 ↔ 571PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8I136.
    PRIDEiQ8I136.

    Interactioni

    Protein-protein interaction databases

    BioGridi45263. 2 interactions.
    DIPiDIP-26941N.
    IntActiQ8I136. 1 interaction.
    MINTiMINT-1107053.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8I136.
    SMRiQ8I136. Positions 90-587.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini32 – 589558LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3120Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini458 – 589132Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 255106Catalytic subdomain AAdd
    BLAST
    Regioni315 – 37763Catalytic subdomain BAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi37 – 7236Pro-richAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239675.
    GeneTreeiENSGT00750000117385.
    HOGENOMiHOG000038227.
    InParanoidiQ8I136.
    KOiK00710.
    OMAiHERTEKD.
    PhylomeDBiQ8I136.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q8I136-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLPRMLKMKT VGTVLAVIWL FGLAFIYVQS TSSSLRPPGR HPPPLPQLDP    50
    LIPQNPPQND EIRPKKSAPP IPTINLAEDT TIHERTEKDV TWKTFDVEKF 100
    LNKGKWHQGE DKYKANSFNQ EASDALNPTR KIPDSREPQC RDVDYSKVGM 150
    QPTTVIITYH NEARSSLLRT VFSVFNQSPE ELLLEIVLVD DNSQDVEIGK 200
    ELAQIQRITV LRNNQREGLI RSRVKGAQVA RAPVLTFLDS HIECNQKWLE 250
    PLLARIAENP KAVVAPIIDV INVDNFNYVG ASADLRGGFD WTLVFRWEFM 300
    NEQLRKERHA HPTAPIRSPT MAGGLFAISK EWFNELGTYD LDMEVWGGEN 350
    LEMSFRVWQC GGSLEIMPCS RVGHVFRKKH PYTFPGGSGN VFQKNTRRAA 400
    EVWMDEYKAI YLKNVPSARF VNFGDITDRL AIRDRLQCKS FKWYLENVYP 450
    QLEIPRKTPG KSFQMKIGNL CLDSMARKES EAPGLFGCHG TGGNQEWVFD 500
    QLTKTFKNAI SQLCLDFSSN TENKTVTMVK CENLRPDTMV VEKNGWLTQG 550
    GKCLTVNQGS GGDWLIYGAH CELNNGAQRW IFEKLDTYE 589
    Length:589
    Mass (Da):67,031
    Last modified:August 16, 2004 - v2
    Checksum:i108D621D91A329D2
    GO
    Isoform b (identifier: Q8I136-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         453-453: E → D
         454-589: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:453
    Mass (Da):51,879
    Checksum:i572763F5D4D25B53
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei453 – 4531E → D in isoform b. CuratedVSP_011236
    Alternative sequencei454 – 589136Missing in isoform b. CuratedVSP_011237Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031834 mRNA. Translation: AAC13670.1.
    AL132943 Genomic DNA. Translation: CAB81985.3.
    AL132943 Genomic DNA. Translation: CAC14394.1.
    PIRiT42244.
    RefSeqiNP_001024216.1. NM_001029045.3. [Q8I136-1]
    NP_507850.2. NM_075449.7. [Q8I136-2]
    UniGeneiCel.19666.

    Genome annotation databases

    EnsemblMetazoaiY116F11B.12a.1; Y116F11B.12a.1; WBGene00001629. [Q8I136-1]
    Y116F11B.12a.2; Y116F11B.12a.2; WBGene00001629. [Q8I136-1]
    GeneIDi180302.
    KEGGicel:CELE_Y116F11B.12.
    UCSCiY116F11B.12b.1. c. elegans. [Q8I136-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031834 mRNA. Translation: AAC13670.1 .
    AL132943 Genomic DNA. Translation: CAB81985.3 .
    AL132943 Genomic DNA. Translation: CAC14394.1 .
    PIRi T42244.
    RefSeqi NP_001024216.1. NM_001029045.3. [Q8I136-1 ]
    NP_507850.2. NM_075449.7. [Q8I136-2 ]
    UniGenei Cel.19666.

    3D structure databases

    ProteinModelPortali Q8I136.
    SMRi Q8I136. Positions 90-587.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 45263. 2 interactions.
    DIPi DIP-26941N.
    IntActi Q8I136. 1 interaction.
    MINTi MINT-1107053.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Proteomic databases

    PaxDbi Q8I136.
    PRIDEi Q8I136.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai Y116F11B.12a.1 ; Y116F11B.12a.1 ; WBGene00001629 . [Q8I136-1 ]
    Y116F11B.12a.2 ; Y116F11B.12a.2 ; WBGene00001629 . [Q8I136-1 ]
    GeneIDi 180302.
    KEGGi cel:CELE_Y116F11B.12.
    UCSCi Y116F11B.12b.1. c. elegans. [Q8I136-1 ]

    Organism-specific databases

    CTDi 180302.
    WormBasei Y116F11B.12a ; CE26046 ; WBGene00001629 ; gly-4.
    Y116F11B.12b ; CE32074 ; WBGene00001629 ; gly-4.

    Phylogenomic databases

    eggNOGi NOG239675.
    GeneTreei ENSGT00750000117385.
    HOGENOMi HOG000038227.
    InParanoidi Q8I136.
    KOi K00710.
    OMAi HERTEKD.
    PhylomeDBi Q8I136.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 908800.
    PROi Q8I136.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
      Hagen F.K., Nehrke K.
      J. Biol. Chem. 273:8268-8277(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), ENZYME ACTIVITY.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGALT4_CAEEL
    AccessioniPrimary (citable) accession number: Q8I136
    Secondary accession number(s): O61390
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3