Q8I0P7 (PDE3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-3
EC=3.1.4.17

Gene names

Name:	pde-3
ORF Names:	E01F3.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate. UniProtKB P54750
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. UniProtKB P54750

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform b Ref.1 (identifier: Q8I0P7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a Ref.1 (identifier: Q8I0P7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-168: Missing.
     169-181: PTVPAEPNKARSS → MNSKVFKSKKNFC
     625-625: Q → QVPFTICCSIAEEEYV

Note: No experimental confirmation available.

Isoform c (identifier: Q8I0P7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.
     52-148: HMQRTFHEAM...PYGGESMVVG → MFPFRRSESH...VIGQQNEGDK
     625-625: Q → QVPFTICCSIAEEEYV

Note: No experimental confirmation available.

Isoform d (identifier: Q8I0P7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     134-181: SVVENPYGGE...PAEPNKARSS → MMSSPSTSRC...RVNSPLTAFA
     625-625: Q → QVPFTICCSIAEEEYV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Probable 3',5'-cyclic phosphodiesterase pde-3		PRO_0000198846
Natural variations
	Alternative sequence	1 – 168	168	Missing in isoform a.		VSP_051737
	Alternative sequence	1 – 133	133	Missing in isoform d.		VSP_038011
	Alternative sequence	1 – 51	51	Missing in isoform c.		VSP_038012
	Alternative sequence	52 – 148	97	HMQRT…SMVVG → MFPFRRSESHSPASRCKSAG LPIATTNGFHSIPSSASSST QSINRQGSVDNLIFKLLTKK YMAKCLGFTPSRSSVAIQTT PVVEVAPVIGQQNEGDK in isoform c.		VSP_038013
	Alternative sequence	134 – 181	48	SVVEN…KARSS → MMSSPSTSRCGHGGVAPADA NGKEIQPFGVLVVNKLQKRV NSPLTAFA in isoform d.		VSP_038014
	Alternative sequence	169 – 181	13	PTVPA…KARSS → MNSKVFKSKKNFC in isoform a.		VSP_051738
	Alternative sequence	625	1	Q → QVPFTICCSIAEEEYV in isoform a, isoform c and isoform d.		VSP_038015

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified September 22, 2009. Version 3. Checksum: BC7FD6472A3F2967	FASTA	625	69,869
	10 20 30 40 50 60 MRKNRDTATS SPASSGVSIQ QRRGSTTQNS GVGSIGLPVL GGRGRGVMSD GHMQRTFHEA 70 80 90 100 110 120 MRQRRTSLPA NSLSLNGAKV TGSSLSEAKG LIADMLMNKE LPGNVASCLR AVTMLLEQRP 130 140 150 160 170 180 LPLNGLLNDF GLPSVVENPY GGESMVVGAS KPRISNITFS TVTSATGLPT VPAEPNKARS 190 200 210 220 230 240 SSYWKTEASP SNNNEHETPV DLLRKISVSR KESGTHVDTV VTTIDGQRYD TRELDTDPDL 250 260 270 280 290 300 AETAVWSFPI FQMSRKHPQT ILSRLTYNIF QQAELFRIFK VSPIKFFNFF HALEKGYWEI 310 320 330 340 350 360 PYHNRIHAAD VLHGCYYLSA HPVRSTFLTP KTPDSVLTPP HPHHQHSSIM SQLSTLELMA 370 380 390 400 410 420 LFTAAAMHDY DHPGRTNAFL VQVEDKKAIL YNDRSVLENH HAAESWKLLN KPENHFIENL 430 440 450 460 470 480 DPAEMKRFRY LVLEYILATD LKQHFEIIMT FTERLTEIDV QVETDRLLIG KLLIKMADIN 490 500 510 520 530 540 SPTKPYGLHR QWTDRICEEF YEQGDDERRR GLPITPYMDR GDAQVAKLQD SFIAHVVSPL 550 560 570 580 590 600 ATAMNECGLL PILPGLDTSE LIINMEHNHR KWKEQIELEN GGSYEAQITC NGGTAVNGVI 610 620 EEESASTSDS PDPRRDSPLD SDLSQ
	Isoform a [UniParc]. Checksum: B531D5A5A24184C5	FASTA	472	54,195
	10 20 30 40 50 60 MNSKVFKSKK NFCSYWKTEA SPSNNNEHET PVDLLRKISV SRKESGTHVD TVVTTIDGQR 70 80 90 100 110 120 YDTRELDTDP DLAETAVWSF PIFQMSRKHP QTILSRLTYN IFQQAELFRI FKVSPIKFFN 130 140 150 160 170 180 FFHALEKGYW EIPYHNRIHA ADVLHGCYYL SAHPVRSTFL TPKTPDSVLT PPHPHHQHSS 190 200 210 220 230 240 IMSQLSTLEL MALFTAAAMH DYDHPGRTNA FLVQVEDKKA ILYNDRSVLE NHHAAESWKL 250 260 270 280 290 300 LNKPENHFIE NLDPAEMKRF RYLVLEYILA TDLKQHFEII MTFTERLTEI DVQVETDRLL 310 320 330 340 350 360 IGKLLIKMAD INSPTKPYGL HRQWTDRICE EFYEQGDDER RRGLPITPYM DRGDAQVAKL 370 380 390 400 410 420 QDSFIAHVVS PLATAMNECG LLPILPGLDT SELIINMEHN HRKWKEQIEL ENGGSYEAQI 430 440 450 460 470 TCNGGTAVNG VIEEESASTS DSPDPRRDSP LDSDLSQVPF TICCSIAEEE YV
	Isoform c [UniParc]. Checksum: F28C41B3D040F21E	FASTA	589	66,388
	10 20 30 40 50 60 MFPFRRSESH SPASRCKSAG LPIATTNGFH SIPSSASSST QSINRQGSVD NLIFKLLTKK 70 80 90 100 110 120 YMAKCLGFTP SRSSVAIQTT PVVEVAPVIG QQNEGDKASK PRISNITFST VTSATGLPTV 130 140 150 160 170 180 PAEPNKARSS SYWKTEASPS NNNEHETPVD LLRKISVSRK ESGTHVDTVV TTIDGQRYDT 190 200 210 220 230 240 RELDTDPDLA ETAVWSFPIF QMSRKHPQTI LSRLTYNIFQ QAELFRIFKV SPIKFFNFFH 250 260 270 280 290 300 ALEKGYWEIP YHNRIHAADV LHGCYYLSAH PVRSTFLTPK TPDSVLTPPH PHHQHSSIMS 310 320 330 340 350 360 QLSTLELMAL FTAAAMHDYD HPGRTNAFLV QVEDKKAILY NDRSVLENHH AAESWKLLNK 370 380 390 400 410 420 PENHFIENLD PAEMKRFRYL VLEYILATDL KQHFEIIMTF TERLTEIDVQ VETDRLLIGK 430 440 450 460 470 480 LLIKMADINS PTKPYGLHRQ WTDRICEEFY EQGDDERRRG LPITPYMDRG DAQVAKLQDS 490 500 510 520 530 540 FIAHVVSPLA TAMNECGLLP ILPGLDTSEL IINMEHNHRK WKEQIELENG GSYEAQITCN 550 560 570 580 GGTAVNGVIE EESASTSDSP DPRRDSPLDS DLSQVPFTIC CSIAEEEYV
	Isoform d [UniParc]. Checksum: 063280420B4B2325	FASTA	507	57,604
	10 20 30 40 50 60 MMSSPSTSRC GHGGVAPADA NGKEIQPFGV LVVNKLQKRV NSPLTAFASY WKTEASPSNN 70 80 90 100 110 120 NEHETPVDLL RKISVSRKES GTHVDTVVTT IDGQRYDTRE LDTDPDLAET AVWSFPIFQM 130 140 150 160 170 180 SRKHPQTILS RLTYNIFQQA ELFRIFKVSP IKFFNFFHAL EKGYWEIPYH NRIHAADVLH 190 200 210 220 230 240 GCYYLSAHPV RSTFLTPKTP DSVLTPPHPH HQHSSIMSQL STLELMALFT AAAMHDYDHP 250 260 270 280 290 300 GRTNAFLVQV EDKKAILYND RSVLENHHAA ESWKLLNKPE NHFIENLDPA EMKRFRYLVL 310 320 330 340 350 360 EYILATDLKQ HFEIIMTFTE RLTEIDVQVE TDRLLIGKLL IKMADINSPT KPYGLHRQWT 370 380 390 400 410 420 DRICEEFYEQ GDDERRRGLP ITPYMDRGDA QVAKLQDSFI AHVVSPLATA MNECGLLPIL 430 440 450 460 470 480 PGLDTSELII NMEHNHRKWK EQIELENGGS YEAQITCNGG TAVNGVIEEE SASTSDSPDP 490 500 RRDSPLDSDL SQVPFTICCS IAEEEYV

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z93376, AL132949 Genomic DNA. Translation: CAB07570.3. AL132949, Z93376 Genomic DNA. Translation: CAC35868.2. Z93376, Z82279, AL132949 Genomic DNA. Translation: CAD59146.3. Z82279, Z93376, AL132949 Genomic DNA. Translation: CAD59150.3. AL132949, Z82279, Z93376 Genomic DNA. Translation: CAD59173.3. Z93376, AL132949 Genomic DNA. Translation: CAT01013.1. Z93376, AL132949 Genomic DNA. Translation: CAT01014.1. AL132949, Z93376 Genomic DNA. Translation: CAT01094.1. AL132949, Z93376 Genomic DNA. Translation: CAT01095.1.
PIR	T20399.
RefSeq	NP_001254452.1. NM_001267523.1. NP_001254454.1. NM_001267525.1. NP_497084.2. NM_064683.5. NP_871943.3. NM_182143.3.
3D structure databases
ProteinModelPortal	Q8I0P7.
SMR	Q8I0P7. Positions 237-573.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8I0P7. 1 interaction.
Proteomic databases
PaxDb	Q8I0P7.
PRIDE	Q8I0P7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	E01F3.1b; E01F3.1b; E01F3.1.
GeneID	183981.
KEGG	cel:CELE_E01F3.1.
UCSC	E01F3.1b. c. elegans.
Organism-specific databases
CTD	183981.
WormBase	E01F3.1a; CE32847; WBGene00008443; pde-3. E01F3.1b; CE43375; WBGene00008443; pde-3. E01F3.1c; CE43353; WBGene00008443; pde-3. E01F3.1d; CE43321; WBGene00008443; pde-3.
Phylogenomic databases
eggNOG	NOG145074.
GeneTree	ENSGT00690000101692.
HOGENOM	HOG000007164.
OMA	KWKEEIE.
Gene expression databases
ArrayExpress	Q8I0P7.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	923070.

Entry information

Entry name

PDE3_CAEEL

Accession

Primary (citable) accession number: Q8I0P7
Secondary accession number(s): B7FAS9, B7FAT0, Q9BH44

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project