Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8HZM5

- GLNA_CANFA

UniProt

Q8HZM5 - GLNA_CANFA

Protein

Glutamine synthetase

Gene

GLUL

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity.By similarity

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Biotin.By similarity
    Magnesium or manganese.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC
    3. glutamate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase, Lyase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKQ8HZM5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase (EC:6.3.1.2)
    Short name:
    GS
    Alternative name(s):
    Glutamate decarboxylase (EC:4.1.1.15)
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLUL
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Chromosome 7

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 373372Glutamine synthetasePRO_0000153137Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei104 – 1041PhosphotyrosineBy similarity

    Post-translational modificationi

    Ubiquitinated by ZNRF1.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ8HZM5.

    Interactioni

    Subunit structurei

    Homooctamer and homotetramer. Interacts with PALMD By similarity.By similarity

    Structurei

    Secondary structure

    1
    373
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 83
    Helixi11 – 188
    Beta strandi26 – 338
    Beta strandi40 – 4910
    Helixi54 – 563
    Beta strandi60 – 634
    Helixi64 – 674
    Beta strandi76 – 8611
    Turni88 – 903
    Beta strandi95 – 1028
    Helixi114 – 1229
    Helixi123 – 1275
    Beta strandi130 – 14011
    Beta strandi144 – 1463
    Beta strandi158 – 1603
    Turni167 – 1693
    Helixi173 – 18614
    Beta strandi190 – 1956
    Beta strandi201 – 21010
    Helixi213 – 23220
    Beta strandi235 – 2373
    Beta strandi245 – 2473
    Beta strandi251 – 2577
    Helixi259 – 2624
    Turni264 – 2663
    Helixi267 – 27812
    Helixi281 – 2877
    Turni290 – 2956
    Helixi296 – 2983
    Turni303 – 3053
    Beta strandi314 – 3163
    Beta strandi321 – 3255
    Helixi327 – 3326
    Beta strandi337 – 3393
    Helixi348 – 35912
    Beta strandi365 – 3673

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2UU7X-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-373[»]
    ProteinModelPortaliQ8HZM5.
    SMRiQ8HZM5. Positions 3-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8HZM5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    GeneTreeiENSGT00390000010047.
    HOGENOMiHOG000061500.
    HOVERGENiHBG005847.
    InParanoidiQ8HZM5.
    KOiK01915.
    OrthoDBiEOG7CZK5G.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8HZM5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE    50
    PKGVEELPEW NFDGSSTFQS EGSNSDMYLV PAAMFRDPFR KDPNKLVFCE 100
    VFKYNRKPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP 150
    SNGFPGPQGP YYCGVGADKA YGRDIVEAHY RACLYAGIKI AGTNAEVMPA 200
    QWEFQIGPCE GIDMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA 250
    GCHTNFSTKA MREENGLKYI EESIEKLSKR HQYHIRAYDP KGGLDNARRL 300
    TGFHETSNIN DFSAGVANRG ASIRIPRTVG QEKKGYFEDR RPSANCDPFS 350
    VTEALIRTCL LNETGDEPFQ YKN 373
    Length:373
    Mass (Da):42,028
    Last modified:January 23, 2007 - v3
    Checksum:iE5AD18AEED3F0C52
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF544242 mRNA. Translation: AAN41001.1.
    AF544243 Genomic DNA. Translation: AAN41002.1.
    UniGeneiCfa.1407.

    Genome annotation databases

    EnsembliENSCAFT00000020795; ENSCAFP00000019307; ENSCAFG00000013102.
    KEGGicfa:403443.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF544242 mRNA. Translation: AAN41001.1 .
    AF544243 Genomic DNA. Translation: AAN41002.1 .
    UniGenei Cfa.1407.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2UU7 X-ray 3.00 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O 2-373 [» ]
    ProteinModelPortali Q8HZM5.
    SMRi Q8HZM5. Positions 3-372.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8HZM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCAFT00000020795 ; ENSCAFP00000019307 ; ENSCAFG00000013102 .
    KEGGi cfa:403443.

    Organism-specific databases

    CTDi 2752.

    Phylogenomic databases

    eggNOGi COG0174.
    GeneTreei ENSGT00390000010047.
    HOGENOMi HOG000061500.
    HOVERGENi HBG005847.
    InParanoidi Q8HZM5.
    KOi K01915.
    OrthoDBi EOG7CZK5G.

    Enzyme and pathway databases

    SABIO-RK Q8HZM5.

    Miscellaneous databases

    EvolutionaryTracei Q8HZM5.
    NextBioi 20816961.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A splice variant acquiring an extra transcript leader region decreases the translation of glutamine synthetase gene."
      Shin D., Park S., Park C.
      Biochem. J. 374:175-184(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

    Entry informationi

    Entry nameiGLNA_CANFA
    AccessioniPrimary (citable) accession number: Q8HZM5
    Secondary accession number(s): Q8HZM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 77 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3