SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8HZK3

- DUOX1_PIG

UniProt

Q8HZK3 - DUOX1_PIG

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Dual oxidase 1
Gene
DUOX1
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.

Catalytic activityi

NAD(P)H + O2 = NAD(P)+ + H2O2.

Enzyme regulationi

The NADPH oxidase activity is calcium-dependent. Peroxidase activity is inhibited by aminobenzohydrazide By similarity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi828 – 839121 Reviewed prediction
Add
BLAST
Calcium bindingi864 – 875122 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. NAD(P)H oxidase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro
  3. heme binding Source: InterPro
  4. peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cuticle development Source: UniProtKB
  2. cytokine-mediated signaling pathway Source: UniProtKB
  3. hormone biosynthetic process Source: UniProtKB-KW
  4. hydrogen peroxide catabolic process Source: UniProtKB-KW
  5. response to cAMP Source: UniProtKB
  6. thyroid hormone generation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Thyroid hormones biosynthesis

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00194.

Protein family/group databases

PeroxiBasei3348. SscDuOx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual oxidase 1 (EC:1.11.1.-, EC:1.6.3.1)
Gene namesi
Name:DUOX1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Apical cell membrane; Multi-pass membrane protein By similarity
Note: Localizes to the apical membrane of epithelial cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 596575Extracellular Reviewed prediction
Add
BLAST
Transmembranei597 – 61721Helical; Reviewed prediction
Add
BLAST
Topological domaini618 – 1046429Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1047 – 106721Helical; Reviewed prediction
Add
BLAST
Topological domaini1068 – 108215Extracellular Reviewed prediction
Add
BLAST
Transmembranei1083 – 110321Helical; Reviewed prediction
Add
BLAST
Topological domaini1104 – 113835Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1139 – 115921Helical; Reviewed prediction
Add
BLAST
Topological domaini1160 – 119031Extracellular Reviewed prediction
Add
BLAST
Transmembranei1191 – 121121Helical; Reviewed prediction
Add
BLAST
Topological domaini1212 – 122817Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1229 – 124921Helical; Reviewed prediction
Add
BLAST
Topological domaini1250 – 12501Extracellular Reviewed prediction
Transmembranei1251 – 127121Helical; Reviewed prediction
Add
BLAST
Topological domaini1272 – 1553282Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 15531532Dual oxidase 1
PRO_0000223345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...) Reviewed prediction
Glycosylationi342 – 3421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi354 – 3541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi534 – 5341N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Specifically expressed in thyroid.1 Publication

Interactioni

Subunit structurei

Interacts with TXNDC11, TPO and CYBA By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005035.

Structurei

3D structure databases

ProteinModelPortaliQ8HZK3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini815 – 85036EF-hand 1
Add
BLAST
Domaini851 – 88636EF-hand 2
Add
BLAST
Domaini895 – 93036EF-hand 3
Add
BLAST
Domaini1089 – 1271183Ferric oxidoreductase
Add
BLAST
Domaini1272 – 1378107FAD-binding FR-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 593568Peroxidase-like; mediates peroxidase activity By similarity
Add
BLAST
Regioni956 – 1250295Interaction with TXNDC11 By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the peroxidase family.
Contains 3 EF-hand domains.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000231774.
HOVERGENiHBG080428.
KOiK13411.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.640.10. 1 hit.
InterProiIPR029595. DUOX1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF37. PTHR11972:SF37. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
PF13499. EF-hand_7. 1 hit.
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS51384. FAD_FR. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8HZK3-1 [UniParc]FASTAAdd to Basket

« Hide

MGFRLALAWT LLVGPWMPMG ARNSISWEVQ RFDGWYNNLM EHKWGSKGSR     50
LQRLVPASYA DGVYQPLGEP HLPNPRDLSN TAMRGPAGQA SLRNRTVLGV 100
FFGYHVLSDL VSIEKPGCPA EFLNIHIPPG DPVFDPHKSG DVVLPFQRSR 150
WDPNTGQSPS NPRDLTNEVT GWLDGSAIYG SSHSWSDELR SFSGGQLASG 200
PDPAFPRQAQ DPLFMWTPPD PATGQRGPQG LYAFGAEQGN REPFLQALGL 250
LWFRYHNLCA QKLAREHPLW GDEELFQHAR KRVIATYQSI TMYEWLPSFL 300
RKMPQEYTGY RPFLDPSISP EFLAASEQFF STMVPPGVYM RNASCHFQGV 350
INRNSSVSRA LRVCNSYWSR EHPNLQRAED VDALLLGMAS QIAEREDHMV 400
VEDVQDFWPG PLKFSRTDHL ASCLQRGRDL GLPSYTKARA RLGLPPVTRW 450
QDINPALSRS DGIVLEATAA LYNQDLSRLE LLPGGLLESY GDPGPLFSTI 500
VLDQFVRLRD GDRYWFENTK NGLFSEKEIA EIRNTSLRDV LVAVTNMTPG 550
ALQPNVFFWH AGDPCPQPRQ LSTKDLPACA PLIMRDYFKG SGFGFGVTIG 600
TLCCFPLVSL LSAWIVAQLR RRNFKRLQVQ NRQSIMCEKL VGGMKALEWQ 650
GRKEPCRPVL VHLQSGQIHV MDGRLSVLRT IQLRPPQQVN LILSSNHGRR 700
TLLLKIPKEY DLVLMFDLEE ERQVMVENLQ SALKESGLSF QEWELREQEL 750
MRAAVTREQR SHLLETFFRH LFSQVLDIDQ ADAGALPLDS SQKVREALTC 800
ELSRAEFAES LGLKPQDMFV ESMFSLADKD GNGYLSFREF LDILVVFMKG 850
SPEEKSRLMF RMYDFDGNGL ISKDEFIRML RSFIEISNNC LSKAQLTEVV 900
ESMFREAGFQ DKQELTWEDF HFMLRDHDSE LRFTQLCVKG VEVPEVIKDL 950
CRRASYISQE KLCPSPRVSA HCPRSNVDVE VELTPWKLQC PTDTDPPQEI 1000
RRRFGKKVTS FQPLLFTEAH REKFQRSRRH QTVQQFKRFV ENYRRHIGCL 1050
AVFYTIAGGL FLERAYYYAF AAHHMGITDT TRVGIILSRG TAASISFMFS 1100
YILLTMCRNL ITFLRETFLN RYVPFDAAVD FHRLIASTAI ILTVLHSAGH 1150
VVNVYLFSIS PLSVLSCLFP GLFHDNGSEF PQKYYWWFFQ TVPGLTGVML 1200
LLILAIMYVF ASHHFRRCSF RGFWLTHHLY ILLYMLLIIH GSFALIQLPR 1250
FHIFFLVPAL IYVGDKLVSL SRKKVEISVV KAELLPSGVT HLQFQRPQGF 1300
EYKSGQWVRI ACLALGTTEY HPFTLTSAPH EDTLSLHIRA AGPWTTRLRE 1350
IYSPPTDDNC AKYPKLYLDG PFGEGHQEWH KFEVSVLVGG GIGVTPFASI 1400
LKDLVFKSSV SCQVFCKKIY FIWVTRTQRQ FEWLADIIRE VEENDHRDLV 1450
SVHIYITQLA EKFDLRTTML YICERHFQKV LNRSLFTGLR SITHFGRPPF 1500
EPFFNSLQEV HPQVRKIGVF SCGPPGMTKN VEKACQLINR QDRTHFSHHY 1550
ENF 1553
Length:1,553
Mass (Da):177,861
Last modified:March 1, 2003 - v1
Checksum:i9F9364322977B710
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF547266 mRNA. Translation: AAN39338.1.
RefSeqiNP_999261.1. NM_214096.2.
UniGeneiSsc.35919.

Genome annotation databases

GeneIDi397177.
KEGGissc:397177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF547266 mRNA. Translation: AAN39338.1 .
RefSeqi NP_999261.1. NM_214096.2.
UniGenei Ssc.35919.

3D structure databases

ProteinModelPortali Q8HZK3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000005035.

Protein family/group databases

PeroxiBasei 3348. SscDuOx01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397177.
KEGGi ssc:397177.

Organism-specific databases

CTDi 53905.

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000231774.
HOVERGENi HBG080428.
KOi K13411.

Enzyme and pathway databases

UniPathwayi UPA00194 .

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.640.10. 1 hit.
InterProi IPR029595. DUOX1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
PANTHERi PTHR11972:SF37. PTHR11972:SF37. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
PF13499. EF-hand_7. 1 hit.
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SMARTi SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF48113. SSF48113. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS51384. FAD_FR. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Effect of iodide on nicotinamide adenine dinucleotide phosphate oxidase activity and Duox2 protein expression in isolated porcine thyroid follicles."
    Morand S., Chaaraoui M., Kaniewski J., Deme D., Ohayon R., Noel-Hudson M.-S., Virion A., Dupuy C.
    Endocrinology 144:1241-1248(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thyroid.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiDUOX1_PIG
AccessioniPrimary (citable) accession number: Q8HZK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi