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Q8HZK3

- DUOX1_PIG

UniProt

Q8HZK3 - DUOX1_PIG

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Protein

Dual oxidase 1

Gene

DUOX1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.

Catalytic activityi

NAD(P)H + O2 = NAD(P)+ + H2O2.

Enzyme regulationi

The NADPH oxidase activity is calcium-dependent. Peroxidase activity is inhibited by aminobenzohydrazide (By similarity).By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi828 – 839121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi864 – 875122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heme binding Source: InterPro
  3. NAD(P)H oxidase activity Source: UniProtKB-EC
  4. peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cuticle development Source: UniProtKB
  2. cytokine-mediated signaling pathway Source: UniProtKB
  3. hormone biosynthetic process Source: UniProtKB-KW
  4. hydrogen peroxide biosynthetic process Source: InterPro
  5. hydrogen peroxide catabolic process Source: UniProtKB-KW
  6. response to cAMP Source: UniProtKB
  7. thyroid hormone generation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Thyroid hormones biosynthesis

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00194.

Protein family/group databases

PeroxiBasei3348. SscDuOx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual oxidase 1 (EC:1.11.1.-, EC:1.6.3.1)
Gene namesi
Name:DUOX1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Apical cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Localizes to the apical membrane of epithelial cells.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 596575ExtracellularSequence AnalysisAdd
BLAST
Transmembranei597 – 61721HelicalSequence AnalysisAdd
BLAST
Topological domaini618 – 1046429CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1047 – 106721HelicalSequence AnalysisAdd
BLAST
Topological domaini1068 – 108215ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1083 – 110321HelicalSequence AnalysisAdd
BLAST
Topological domaini1104 – 113835CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1139 – 115921HelicalSequence AnalysisAdd
BLAST
Topological domaini1160 – 119031ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1191 – 121121HelicalSequence AnalysisAdd
BLAST
Topological domaini1212 – 122817CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1229 – 124921HelicalSequence AnalysisAdd
BLAST
Topological domaini1250 – 12501ExtracellularSequence Analysis
Transmembranei1251 – 127121HelicalSequence AnalysisAdd
BLAST
Topological domaini1272 – 1553282CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 15531532Dual oxidase 1PRO_0000223345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi534 – 5341N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Specifically expressed in thyroid.1 Publication

Interactioni

Subunit structurei

Interacts with TXNDC11, TPO and CYBA.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005035.

Structurei

3D structure databases

ProteinModelPortaliQ8HZK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini815 – 85036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini851 – 88636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini895 – 93036EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini1089 – 1271183Ferric oxidoreductaseAdd
BLAST
Domaini1272 – 1378107FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 593568Peroxidase-like; mediates peroxidase activityBy similarityAdd
BLAST
Regioni956 – 1250295Interaction with TXNDC11By similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the peroxidase family.Curated
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000231774.
HOVERGENiHBG080428.
InParanoidiQ8HZK3.
KOiK13411.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.640.10. 1 hit.
InterProiIPR029595. DUOX1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF37. PTHR11972:SF37. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
PF13499. EF-hand_7. 1 hit.
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS51384. FAD_FR. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8HZK3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFRLALAWT LLVGPWMPMG ARNSISWEVQ RFDGWYNNLM EHKWGSKGSR
60 70 80 90 100
LQRLVPASYA DGVYQPLGEP HLPNPRDLSN TAMRGPAGQA SLRNRTVLGV
110 120 130 140 150
FFGYHVLSDL VSIEKPGCPA EFLNIHIPPG DPVFDPHKSG DVVLPFQRSR
160 170 180 190 200
WDPNTGQSPS NPRDLTNEVT GWLDGSAIYG SSHSWSDELR SFSGGQLASG
210 220 230 240 250
PDPAFPRQAQ DPLFMWTPPD PATGQRGPQG LYAFGAEQGN REPFLQALGL
260 270 280 290 300
LWFRYHNLCA QKLAREHPLW GDEELFQHAR KRVIATYQSI TMYEWLPSFL
310 320 330 340 350
RKMPQEYTGY RPFLDPSISP EFLAASEQFF STMVPPGVYM RNASCHFQGV
360 370 380 390 400
INRNSSVSRA LRVCNSYWSR EHPNLQRAED VDALLLGMAS QIAEREDHMV
410 420 430 440 450
VEDVQDFWPG PLKFSRTDHL ASCLQRGRDL GLPSYTKARA RLGLPPVTRW
460 470 480 490 500
QDINPALSRS DGIVLEATAA LYNQDLSRLE LLPGGLLESY GDPGPLFSTI
510 520 530 540 550
VLDQFVRLRD GDRYWFENTK NGLFSEKEIA EIRNTSLRDV LVAVTNMTPG
560 570 580 590 600
ALQPNVFFWH AGDPCPQPRQ LSTKDLPACA PLIMRDYFKG SGFGFGVTIG
610 620 630 640 650
TLCCFPLVSL LSAWIVAQLR RRNFKRLQVQ NRQSIMCEKL VGGMKALEWQ
660 670 680 690 700
GRKEPCRPVL VHLQSGQIHV MDGRLSVLRT IQLRPPQQVN LILSSNHGRR
710 720 730 740 750
TLLLKIPKEY DLVLMFDLEE ERQVMVENLQ SALKESGLSF QEWELREQEL
760 770 780 790 800
MRAAVTREQR SHLLETFFRH LFSQVLDIDQ ADAGALPLDS SQKVREALTC
810 820 830 840 850
ELSRAEFAES LGLKPQDMFV ESMFSLADKD GNGYLSFREF LDILVVFMKG
860 870 880 890 900
SPEEKSRLMF RMYDFDGNGL ISKDEFIRML RSFIEISNNC LSKAQLTEVV
910 920 930 940 950
ESMFREAGFQ DKQELTWEDF HFMLRDHDSE LRFTQLCVKG VEVPEVIKDL
960 970 980 990 1000
CRRASYISQE KLCPSPRVSA HCPRSNVDVE VELTPWKLQC PTDTDPPQEI
1010 1020 1030 1040 1050
RRRFGKKVTS FQPLLFTEAH REKFQRSRRH QTVQQFKRFV ENYRRHIGCL
1060 1070 1080 1090 1100
AVFYTIAGGL FLERAYYYAF AAHHMGITDT TRVGIILSRG TAASISFMFS
1110 1120 1130 1140 1150
YILLTMCRNL ITFLRETFLN RYVPFDAAVD FHRLIASTAI ILTVLHSAGH
1160 1170 1180 1190 1200
VVNVYLFSIS PLSVLSCLFP GLFHDNGSEF PQKYYWWFFQ TVPGLTGVML
1210 1220 1230 1240 1250
LLILAIMYVF ASHHFRRCSF RGFWLTHHLY ILLYMLLIIH GSFALIQLPR
1260 1270 1280 1290 1300
FHIFFLVPAL IYVGDKLVSL SRKKVEISVV KAELLPSGVT HLQFQRPQGF
1310 1320 1330 1340 1350
EYKSGQWVRI ACLALGTTEY HPFTLTSAPH EDTLSLHIRA AGPWTTRLRE
1360 1370 1380 1390 1400
IYSPPTDDNC AKYPKLYLDG PFGEGHQEWH KFEVSVLVGG GIGVTPFASI
1410 1420 1430 1440 1450
LKDLVFKSSV SCQVFCKKIY FIWVTRTQRQ FEWLADIIRE VEENDHRDLV
1460 1470 1480 1490 1500
SVHIYITQLA EKFDLRTTML YICERHFQKV LNRSLFTGLR SITHFGRPPF
1510 1520 1530 1540 1550
EPFFNSLQEV HPQVRKIGVF SCGPPGMTKN VEKACQLINR QDRTHFSHHY

ENF
Length:1,553
Mass (Da):177,861
Last modified:March 1, 2003 - v1
Checksum:i9F9364322977B710
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF547266 mRNA. Translation: AAN39338.1.
RefSeqiNP_999261.1. NM_214096.2.
UniGeneiSsc.35919.

Genome annotation databases

GeneIDi397177.
KEGGissc:397177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF547266 mRNA. Translation: AAN39338.1 .
RefSeqi NP_999261.1. NM_214096.2.
UniGenei Ssc.35919.

3D structure databases

ProteinModelPortali Q8HZK3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000005035.

Protein family/group databases

PeroxiBasei 3348. SscDuOx01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397177.
KEGGi ssc:397177.

Organism-specific databases

CTDi 53905.

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000231774.
HOVERGENi HBG080428.
InParanoidi Q8HZK3.
KOi K13411.

Enzyme and pathway databases

UniPathwayi UPA00194 .

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.640.10. 1 hit.
InterProi IPR029595. DUOX1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
PANTHERi PTHR11972:SF37. PTHR11972:SF37. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
PF13499. EF-hand_7. 1 hit.
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SMARTi SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF48113. SSF48113. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS51384. FAD_FR. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Effect of iodide on nicotinamide adenine dinucleotide phosphate oxidase activity and Duox2 protein expression in isolated porcine thyroid follicles."
    Morand S., Chaaraoui M., Kaniewski J., Deme D., Ohayon R., Noel-Hudson M.-S., Virion A., Dupuy C.
    Endocrinology 144:1241-1248(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thyroid.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiDUOX1_PIG
AccessioniPrimary (citable) accession number: Q8HZK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3