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Q8HYZ4

- PCKGC_BOVIN

UniProt

Q8HYZ4 - PCKGC_BOVIN

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Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PPCK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Binds 1 manganese ion per subunit.By similarity

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871SubstrateBy similarity
Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
Metal bindingi244 – 2441ManganeseBy similarity
Binding sitei244 – 2441SubstrateBy similarity
Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
Binding sitei286 – 2861SubstrateBy similarity
Active sitei288 – 2881By similarity
Metal bindingi311 – 3111ManganeseBy similarity
Binding sitei405 – 4051GTPBy similarity
Binding sitei436 – 4361GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi287 – 2926GTPBy similarity
Nucleotide bindingi530 – 5334GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. internal protein amino acid acetylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
Short name:
PEPCK-C
Gene namesi
Name:PPCK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; by p300/EP300By similarity
Modified residuei71 – 711N6-acetyllysine; by p300/EP300By similarity
Modified residuei594 – 5941N6-acetyllysine; by p300/EP300By similarity

Post-translational modificationi

Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 (By similarity).By similarity
Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ8HYZ4.
PRIDEiQ8HYZ4.

Expressioni

Inductioni

Regulated by insulin, cAMP and dexamethasone.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ8HYZ4.
SMRiQ8HYZ4. Positions 10-622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni403 – 4053Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8HYZ4.
KOiK01596.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8HYZ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPQLSDGLN YSAKIVRGSL DSLPQAVRSF VESSAKLCRP DQVHICDGSE
60 70 80 90 100
EENRQLLSHM EEEGVIKRLK KYDNCWLALT DPRDVARIES KTVIITREQR
110 120 130 140 150
DTVPIPKNGL SQLGRWMSEE DFEKAFNIRF PGCMKGRTMY VIPFSMGPLG
160 170 180 190 200
SPLSKIGIEL TDSPYVVTSM RIMTRMGTSV LEALGDGEFV KCLHSVGCPL
210 220 230 240 250
PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRM
260 270 280 290 300
ASRLAKEEGW LAEHMLILGI TNPKGQKKYF AAAFPSACGK TNLAMMNPTL
310 320 330 340 350
PGWKVECVGD DIAWMKFDQQ GNLRAINPEN GFFGVAPGTS VRTNPNAIKT
360 370 380 390 400
IQKNTIFTNV AETSDGGVYW EGIDQPLAAR VKLISWKGKE WDPKDGEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIIDPDW ESPEGVPIEG IIFGGRRPVG VPLVYEALSW
460 470 480 490 500
QHGVFVGAAM RSEATAAAEY KGKVIMHDPF AMGPFFGYNF GQYLAHWLSM
510 520 530 540 550
AQRPAAKLPK IFHVNWFRKD KAGRFLWPGF GENSRVLEWM FNRVVGEGGA
560 570 580 590 600
SVTPIGYIPD EDALDLRGLG DVDVKELFHI SKEFWEEEVE EIQKYLEEQV
610 620
NVDLPPEIKN QVLALKQRIS QM
Length:622
Mass (Da):69,353
Last modified:March 1, 2003 - v1
Checksum:iDD7B678E3728992B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY145503 mRNA. Translation: AAN61102.1.
RefSeqiNP_777162.1. NM_174737.2.
UniGeneiBt.12768.

Genome annotation databases

GeneIDi282855.
KEGGibta:282855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY145503 mRNA. Translation: AAN61102.1 .
RefSeqi NP_777162.1. NM_174737.2.
UniGenei Bt.12768.

3D structure databases

ProteinModelPortali Q8HYZ4.
SMRi Q8HYZ4. Positions 10-622.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8HYZ4.
PRIDEi Q8HYZ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 282855.
KEGGi bta:282855.

Organism-specific databases

CTDi 5105.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
HOVERGENi HBG053651.
InParanoidi Q8HYZ4.
KOi K01596.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Miscellaneous databases

NextBioi 20806384.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation."
    Agca C., Greenfield R.B., Hartwell J.R., Donkin S.S.
    Physiol. Genomics 11:53-63(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Developmental regulation and tissue-specific expression of a chimaeric phosphoenolpyruvate carboxykinase/bovine growth hormone gene in transgenic animals."
    McGrane M.M., Yun J.S., Roesler W.J., Park E.A., Wagner T.E., Hanson R.W.
    J. Reprod. Fertil. Suppl. 41:17-23(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPCKGC_BOVIN
AccessioniPrimary (citable) accession number: Q8HYZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3