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Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PCK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.By similarity

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Metal bindingi244ManganeseBy similarity1
Metal bindingi264Manganese; via tele nitrogenBy similarity1
Binding sitei286SubstrateBy similarity1
Active sitei288By similarity1
Metal bindingi311ManganeseBy similarity1
Binding sitei405GTPBy similarity1
Binding sitei436GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi287 – 292GTPBy similarity6
Nucleotide bindingi530 – 533GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32By similarity)
Short name:
PEPCK-C
Gene namesi
Name:PCK1By similarity
Synonyms:PPCK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036261 – 622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineBy similarity1
Modified residuei70N6-acetyllysine; by p300/EP300By similarity1
Modified residuei71N6-acetyllysine; by p300/EP300By similarity1
Modified residuei118PhosphoserineBy similarity1
Modified residuei178PhosphothreonineBy similarity1
Modified residuei286PhosphoserineBy similarity1
Modified residuei594N6-acetyllysine; by p300/EP300By similarity1

Post-translational modificationi

Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 (By similarity).By similarity
Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8HYZ4
PeptideAtlasiQ8HYZ4
PRIDEiQ8HYZ4

Expressioni

Inductioni

Regulated by insulin, cAMP and dexamethasone.1 Publication

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002520

Structurei

3D structure databases

ProteinModelPortaliQ8HYZ4
SMRiQ8HYZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237Substrate bindingBy similarity3
Regioni403 – 405Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
HOGENOMiHOG000191700
HOVERGENiHBG053651
InParanoidiQ8HYZ4
KOiK01596

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.102 hits
3.90.228.202 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8HYZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPQLSDGLN YSAKIVRGSL DSLPQAVRSF VESSAKLCRP DQVHICDGSE
60 70 80 90 100
EENRQLLSHM EEEGVIKRLK KYDNCWLALT DPRDVARIES KTVIITREQR
110 120 130 140 150
DTVPIPKNGL SQLGRWMSEE DFEKAFNIRF PGCMKGRTMY VIPFSMGPLG
160 170 180 190 200
SPLSKIGIEL TDSPYVVTSM RIMTRMGTSV LEALGDGEFV KCLHSVGCPL
210 220 230 240 250
PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRM
260 270 280 290 300
ASRLAKEEGW LAEHMLILGI TNPKGQKKYF AAAFPSACGK TNLAMMNPTL
310 320 330 340 350
PGWKVECVGD DIAWMKFDQQ GNLRAINPEN GFFGVAPGTS VRTNPNAIKT
360 370 380 390 400
IQKNTIFTNV AETSDGGVYW EGIDQPLAAR VKLISWKGKE WDPKDGEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIIDPDW ESPEGVPIEG IIFGGRRPVG VPLVYEALSW
460 470 480 490 500
QHGVFVGAAM RSEATAAAEY KGKVIMHDPF AMGPFFGYNF GQYLAHWLSM
510 520 530 540 550
AQRPAAKLPK IFHVNWFRKD KAGRFLWPGF GENSRVLEWM FNRVVGEGGA
560 570 580 590 600
SVTPIGYIPD EDALDLRGLG DVDVKELFHI SKEFWEEEVE EIQKYLEEQV
610 620
NVDLPPEIKN QVLALKQRIS QM
Length:622
Mass (Da):69,353
Last modified:March 1, 2003 - v1
Checksum:iDD7B678E3728992B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145503 mRNA Translation: AAN61102.1
RefSeqiNP_777162.1, NM_174737.2
UniGeneiBt.12768

Genome annotation databases

GeneIDi282855
KEGGibta:282855

Similar proteinsi

Entry informationi

Entry nameiPCKGC_BOVIN
AccessioniPrimary (citable) accession number: Q8HYZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome