Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8HYZ4

- PCKGC_BOVIN

UniProt

Q8HYZ4 - PCKGC_BOVIN

Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PPCK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Enzyme regulationi

    Enzyme activity is enhanced by acetylation.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi244 – 2441ManganeseBy similarity
    Binding sitei244 – 2441SubstrateBy similarity
    Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
    Binding sitei286 – 2861SubstrateBy similarity
    Active sitei288 – 2881By similarity
    Metal bindingi311 – 3111ManganeseBy similarity
    Binding sitei405 – 4051GTPBy similarity
    Binding sitei436 – 4361GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi287 – 2926GTPBy similarity
    Nucleotide bindingi530 – 5334GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. internal protein amino acid acetylation Source: UniProtKB

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK-C
    Gene namesi
    Name:PPCK1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103626Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei70 – 701N6-acetyllysine; by p300/EP300By similarity
    Modified residuei71 – 711N6-acetyllysine; by p300/EP300By similarity
    Modified residuei594 – 5941N6-acetyllysine; by p300/EP300By similarity

    Post-translational modificationi

    Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 By similarity.By similarity
    Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PaxDbiQ8HYZ4.
    PRIDEiQ8HYZ4.

    Expressioni

    Inductioni

    Regulated by insulin, cAMP and dexamethasone.1 Publication

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8HYZ4.
    SMRiQ8HYZ4. Positions 10-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni403 – 4053Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    HOVERGENiHBG053651.
    InParanoidiQ8HYZ4.
    KOiK01596.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8HYZ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPQLSDGLN YSAKIVRGSL DSLPQAVRSF VESSAKLCRP DQVHICDGSE    50
    EENRQLLSHM EEEGVIKRLK KYDNCWLALT DPRDVARIES KTVIITREQR 100
    DTVPIPKNGL SQLGRWMSEE DFEKAFNIRF PGCMKGRTMY VIPFSMGPLG 150
    SPLSKIGIEL TDSPYVVTSM RIMTRMGTSV LEALGDGEFV KCLHSVGCPL 200
    PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRM 250
    ASRLAKEEGW LAEHMLILGI TNPKGQKKYF AAAFPSACGK TNLAMMNPTL 300
    PGWKVECVGD DIAWMKFDQQ GNLRAINPEN GFFGVAPGTS VRTNPNAIKT 350
    IQKNTIFTNV AETSDGGVYW EGIDQPLAAR VKLISWKGKE WDPKDGEPCA 400
    HPNSRFCTPA SQCPIIDPDW ESPEGVPIEG IIFGGRRPVG VPLVYEALSW 450
    QHGVFVGAAM RSEATAAAEY KGKVIMHDPF AMGPFFGYNF GQYLAHWLSM 500
    AQRPAAKLPK IFHVNWFRKD KAGRFLWPGF GENSRVLEWM FNRVVGEGGA 550
    SVTPIGYIPD EDALDLRGLG DVDVKELFHI SKEFWEEEVE EIQKYLEEQV 600
    NVDLPPEIKN QVLALKQRIS QM 622
    Length:622
    Mass (Da):69,353
    Last modified:March 1, 2003 - v1
    Checksum:iDD7B678E3728992B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY145503 mRNA. Translation: AAN61102.1.
    RefSeqiNP_777162.1. NM_174737.2.
    UniGeneiBt.12768.

    Genome annotation databases

    GeneIDi282855.
    KEGGibta:282855.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY145503 mRNA. Translation: AAN61102.1 .
    RefSeqi NP_777162.1. NM_174737.2.
    UniGenei Bt.12768.

    3D structure databases

    ProteinModelPortali Q8HYZ4.
    SMRi Q8HYZ4. Positions 10-622.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8HYZ4.
    PRIDEi Q8HYZ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 282855.
    KEGGi bta:282855.

    Organism-specific databases

    CTDi 5105.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    HOVERGENi HBG053651.
    InParanoidi Q8HYZ4.
    KOi K01596.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Miscellaneous databases

    NextBioi 20806384.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation."
      Agca C., Greenfield R.B., Hartwell J.R., Donkin S.S.
      Physiol. Genomics 11:53-63(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Developmental regulation and tissue-specific expression of a chimaeric phosphoenolpyruvate carboxykinase/bovine growth hormone gene in transgenic animals."
      McGrane M.M., Yun J.S., Roesler W.J., Park E.A., Wagner T.E., Hanson R.W.
      J. Reprod. Fertil. Suppl. 41:17-23(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiPCKGC_BOVIN
    AccessioniPrimary (citable) accession number: Q8HYZ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3