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Protein

Induced myeloid leukemia cell differentiation protein Mcl-1 homolog

Gene

MCL1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
Gene namesi
Name:MCL1
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei327 – 34923HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Induced myeloid leukemia cell differentiation protein Mcl-1 homologPRO_0000143078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki5 – 5Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei121 – 1211PhosphoserineBy similarity
Cross-linki136 – 136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei159 – 1591Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
Modified residuei162 – 1621PhosphoserineBy similarity
Modified residuei163 – 1631PhosphothreonineBy similarity
Cross-linki194 – 194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki197 – 197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C-terminal fragment.By similarity
Rapidly degraded in the absence of phosphorylation in the PEST region.By similarity
Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites (By similarity).By similarity
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei127 – 1282Cleavage; by caspase-3By similarity
Sitei157 – 1582Cleavage; by caspase-3By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Tissue specificityi

Detected in peripheral blood mononuclear cells and bone marrow.1 Publication

Interactioni

Subunit structurei

Interacts with HIF3A (via C-terminus domain). Interacts with BAD, BOK, BIK, BFM, BAX, BAK1, BBC3, TPT1, PMAIP1 and BOP. Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ8HYS5.
SMRiQ8HYS5. Positions 172-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 17572PEST-likeBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi209 – 22315BH3Add
BLAST
Motifi252 – 27221BH1Add
BLAST
Motifi304 – 31916BH2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 6246Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000232116.
HOVERGENiHBG003527.
InParanoidiQ8HYS5.

Family and domain databases

InterProiIPR013281. Apop_reg_Mc1.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01866. APOPREGMCL1.
PR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8HYS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGLKRNAVI RTQLYCGGAG LGAGSGGASS SGGRLLASGR EATTRREGGG
60 70 80 90 100
GEAGAVIGGS AGASPPTTLA PDARRVARPS PIGAEGPNVS ATPPRLLLLA
110 120 130 140 150
PPCRASPPEE MEGPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGEA
160 170 180 190 200
SSGPGMDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDAKPLG
210 220 230 240 250
GSRAASRKAL ETLQRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVI
260 270 280 290 300
VHVFSDGVTN WGRIVTLISF GAFVAKHLKS INQESCIEPL AESITDVLVR
310 320 330 340 350
TKRDWLVKQR GWDGFVEFFH VEDLEGGIRN VLLAFAGVAG VGAGLAYLIR
Length:350
Mass (Da):36,837
Last modified:March 1, 2003 - v1
Checksum:i6E17DED8F55A9278
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093582 mRNA. Translation: BAC21258.1.
UniGeneiCfa.34.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093582 mRNA. Translation: BAC21258.1.
UniGeneiCfa.34.

3D structure databases

ProteinModelPortaliQ8HYS5.
SMRiQ8HYS5. Positions 172-321.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000232116.
HOVERGENiHBG003527.
InParanoidiQ8HYS5.

Miscellaneous databases

NextBioi20817048.

Family and domain databases

InterProiIPR013281. Apop_reg_Mc1.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01866. APOPREGMCL1.
PR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of canine Mcl-1 gene and its expression in tumor cell lines."
    Sano J., Oguma K., Kano R., Hasegawa A.
    J. Vet. Med. Sci. 66:709-712(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Blood.

Entry informationi

Entry nameiMCL1_CANLF
AccessioniPrimary (citable) accession number: Q8HYS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.