Reviewed, UniProtKB/Swiss-Prot Q8HYL8 (ACOX1_PHACI)

Names and origin

Protein names

Recommended name:
    Peroxisomal acyl-coenzyme A oxidase 1
      Short name=AOX
    EC=1.3.3.6
Alternative name(s):
    Palmitoyl-CoA oxidase

Gene names

Name:

ACOX1

Organism

Phascolarctos cinereus (Koala)

Taxonomic identifier

38626 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Diprotodontia › Phascolarctidae › Phascolarctos

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the desaturation of very long chain acyl-CoAs to 2-trans-enoyl-CoAs.
Catalytic activity	Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; peroxisomal fatty acid beta-oxidation.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the acyl-CoA oxidase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid beta-oxidation Inferred from electronic annotation. Source: InterPro generation of precursor metabolites and energy Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW prostaglandin metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	peroxisome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro acyl-CoA oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8HYL8-1)

Also known as: AOX1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8HYL8-2)

Also known as: AOX2;

The sequence of this isoform differs from the canonical sequence as follows:
     90-131: NFVHRGRPEP...RFFIPAWNLE → KPQLINFVEP...KWLPPTQGLQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	Peroxisomal acyl-coenzyme A oxidase 1		PRO_0000204679
Regions
	Motif	659 – 661	3	Microbody targeting signal
Sites
	Active site	421	1	Proton acceptor By similarity
	Binding site	139	1	FAD By similarity
	Binding site	178	1	FAD; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	255	1	N6-acetyllysine By similarity
	Modified residue	267	1	N6-acetyllysine By similarity
	Modified residue	310	1	Phosphoserine By similarity
	Modified residue	437	1	N6-acetyllysine By similarity
	Modified residue	643	1	N6-acetyllysine By similarity
	Modified residue	649	1	Phosphoserine By similarity
	Modified residue	652	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	90 – 131	42	NFVHR…AWNLE → KPQLINFVEPVGLTYSMFIP TLLDQGTTAQQQKWLPPTQG LQ in isoform 2.		VSP_008029
Experimental info
	Sequence conflict	313	1	Q → R in AAO15577. Ref.1
	Sequence conflict	360	1	S → N in AAO15577. Ref.1
	Sequence conflict	374	1	T → A in AAO15577. Ref.1
	Sequence conflict	528	1	E → G in AAO15577. Ref.1
	Sequence conflict	564	1	L → P in AAO15577. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (AOX1) [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A3BE9AC7C68E4DB8	FASTA	661	74,976
	10 20 30 40 50 60 MNPDLRRERD AASFDAEKLT YILDGGSERT RRRREIENLI LNDPDFKHED LNFLTRSERY 70 80 90 100 110 120 EIAVRKSATM VKKMRDFGIA DPEEIMWFKN FVHRGRPEPL DLHLGMFLPT LLHQATQEQQ 130 140 150 160 170 180 ERFFIPAWNL EIIGTYAQTE MGHGTHLRGL ETTATYDPKT QEFILNSPTV TSIKWWPGGL 190 200 210 220 230 240 GKTSNHAIVL AQLYTKGECY GLHAFIVPIR EMGTHKPFPG IIVGDIGPKF GYDEMDNGYL 250 260 270 280 290 300 KMDNYRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RSLSKACTIA 310 320 330 340 350 360 IRYSLIRHQS EIQPGDPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINVDIS 370 380 390 400 410 420 QGNLNELPEL HALTAGLKAF TSWTTNSGIE ACRMACGGHG YSHCSGLPNI YVTFTPTCTF 430 440 450 460 470 480 EGENTVMMLQ TARFLMKSYD QVHSGKLVGG MVSYLNDLPS QRIQPQQVAA WPAMVDINNP 490 500 510 520 530 540 DSLTEAYKHR AARLVEAAAR NLQAEMKHRK SKEIAWNFTS VDLVRASEAH CHYVVVKLFS 550 560 570 580 590 600 GNLSKIDDKP IQAVLTNLCL LYALYGISQN SGDFLQGGIL TESQLTQVNQ RVKELLTLIR 610 620 630 640 650 660 PESAALVDAF DFQDVSLGSV LGRYDGNIYE NMFEWAKKSP LNKSEVHESY HKHLKPLQSK L
	Isoform 2 (AOX2). Checksum: 59A247785D86047E	FASTA	661	74,676
	10 20 30 40 50 60 MNPDLRRERD AASFDAEKLT YILDGGSERT RRRREIENLI LNDPDFKHED LNFLTRSERY 70 80 90 100 110 120 EIAVRKSATM VKKMRDFGIA DPEEIMWFKK PQLINFVEPV GLTYSMFIPT LLDQGTTAQQ 130 140 150 160 170 180 QKWLPPTQGL QIIGTYAQTE MGHGTHLRGL ETTATYDPKT QEFILNSPTV TSIKWWPGGL 190 200 210 220 230 240 GKTSNHAIVL AQLYTKGECY GLHAFIVPIR EMGTHKPFPG IIVGDIGPKF GYDEMDNGYL 250 260 270 280 290 300 KMDNYRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RSLSKACTIA 310 320 330 340 350 360 IRYSLIRHQS EIQPGDPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINVDIS 370 380 390 400 410 420 QGNLNELPEL HALTAGLKAF TSWTTNSGIE ACRMACGGHG YSHCSGLPNI YVTFTPTCTF 430 440 450 460 470 480 EGENTVMMLQ TARFLMKSYD QVHSGKLVGG MVSYLNDLPS QRIQPQQVAA WPAMVDINNP 490 500 510 520 530 540 DSLTEAYKHR AARLVEAAAR NLQAEMKHRK SKEIAWNFTS VDLVRASEAH CHYVVVKLFS 550 560 570 580 590 600 GNLSKIDDKP IQAVLTNLCL LYALYGISQN SGDFLQGGIL TESQLTQVNQ RVKELLTLIR 610 620 630 640 650 660 PESAALVDAF DFQDVSLGSV LGRYDGNIYE NMFEWAKKSP LNKSEVHESY HKHLKPLQSK L

References

[1]

"Identification and cloning of two forms of liver peroxisomal fatty Acyl CoA oxidase from the koala (Phascolarctos cinereus)." Ngo S.N.T., McKinnon R.A., Stupans I. Gene 309:91-99(2003) [PubMed: 12758125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF463453 mRNA. Translation: AAO15576.1. AF463454 mRNA. Translation: AAO15577.1.
3D structure databases
HSSP	HSSP built from PDB template 1IS2 based on UniProtKB P07872.
SMR	Q8HYL8. Positions 1-655.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8HYL8.
Enzyme and pathway databases
BRENDA	1.3.3.6. 190701.
Family and domain databases
InterPro	IPR006091. Acyl-CoA_Oxase/DH_M. IPR012258. Acyl-CoA_oxidase. IPR002655. Acyl-CoA_oxidase_C. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 2 hits.
PANTHER	PTHR10909:SF11. Acyl-CoA_oxidase. 1 hit.
Pfam	PF01756. ACOX. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. [Graphical view]
PIRSF	PIRSF000168. Acyl-CoA_oxidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACOX1_PHACI

Accession

Primary (citable) accession number: Q8HYL8
Secondary accession number(s): Q8HYL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)