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Protein

Dimethylaniline monooxygenase [N-oxide-forming] 3

Gene

FMO3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide.1 Publication

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
N,N,N-trimethylamine + NADPH + O2 = N,N,N-trimethylamine N-oxide + NADP+ + H2O.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13FADBy similarity5
Nucleotide bindingi40 – 41FADBy similarity2
Nucleotide bindingi60 – 61NADPBy similarity2
Nucleotide bindingi61 – 62FADBy similarity2
Nucleotide bindingi195 – 198NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-BTA-217271 FMO oxidises nucleophiles

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 3
Hepatic flavin-containing monooxygenase 3
Short name:
FMO 3
Trimethylamine monooxygenase (EC:1.14.13.148)
Gene namesi
Name:FMO3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Organism-specific databases

VGNCiVGNC:29051 FMO3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei510 – 530HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Involvement in diseasei

Defects in FMO3 are the cause of a fishy off-flavor in cow milk due to by an elevated level of trimethylamine (TMA) in body fluids.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476521 – 532Dimethylaniline monooxygenase [N-oxide-forming] 3Add BLAST532

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8HYJ9
PeptideAtlasiQ8HYJ9
PRIDEiQ8HYJ9

Expressioni

Gene expression databases

BgeeiENSBTAG00000020597
ExpressionAtlasiQ8HYJ9 baseline

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027444

Structurei

3D structure databases

ProteinModelPortaliQ8HYJ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1399 Eukaryota
COG2072 LUCA
GeneTreeiENSGT00760000119232
HOGENOMiHOG000076537
HOVERGENiHBG002037
InParanoidiQ8HYJ9
KOiK00485
OMAiAISDWWY
OrthoDBiEOG091G0465
TreeFamiTF105285

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000960 Flavin_mOase
IPR020946 Flavin_mOase-like
IPR002255 Flavin_mOase_3
PfamiView protein in Pfam
PF00743 FMO-like, 1 hit
PIRSFiPIRSF000332 FMO, 1 hit
PRINTSiPR00370 FMOXYGENASE
PR01123 FMOXYGENASE3
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Q8HYJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKVAIIGA GISGLASIRN CLEEGLEPTC FEKGEDIGGL WKFSDHVEEG
60 70 80 90 100
RASIYRSVFT NSSKEMTCFP DFPFPDDFPN FMHNSKLQEY ITMFAKEKNL
110 120 130 140 150
LKYIQFKTIV SSVNKRPDFQ TTGQWDVITE KDGKKESAVF DAVMICSGHH
160 170 180 190 200
VYPNIPKESF PGIKLFKGKC FHSRDYKEPG IFKGKRVLVI GLGNSGCDIA
210 220 230 240 250
SELSHIAEKV IISSRSGSWV MSRVWDEGYP WDMLFITRFE TFLKNTLPTV
260 270 280 290 300
ISNWWYMKQM NARFKHENYG LMPLNSTLRK EPVFNDELPA CILCGIVTIK
310 320 330 340 350
PNVKEFTEDS AIFEDGTVFK AIDYVIFATG YSYAYPFLDD SIIKSRDNEV
360 370 380 390 400
TLFKGIFPPP LEKPTLAVIG LVQSLGAAIP TTDLQSRWAV QVIKGTCPLP
410 420 430 440 450
SVKDMMNDID EKMGKKLKLF GKSDTIQTDY VVYMDELASF IGAKPNIPWL
460 470 480 490 500
FLTDPKLALE VYFGPCTPYQ FRLVGPGKWP GARNAILTQW DRLLKPMTTR
510 520 530
VVGSPLKPCL FCNWFRPVLI SVVSIAALIV LF
Length:532
Mass (Da):60,093
Last modified:March 1, 2003 - v1
Checksum:i2CA5964889490CBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF488422 mRNA Translation: AAN27919.1
RefSeqiNP_776482.1, NM_174057.2
UniGeneiBt.12519

Genome annotation databases

EnsembliENSBTAT00000027444; ENSBTAP00000027444; ENSBTAG00000020597
GeneIDi281167
KEGGibta:281167

Similar proteinsi

Entry informationi

Entry nameiFMO3_BOVIN
AccessioniPrimary (citable) accession number: Q8HYJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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