Reviewed, UniProtKB/Swiss-Prot Q8HYJ9 (FMO3_BOVIN)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 3
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 3
      Short name=FMO 3
    Dimethylaniline oxidase 3

Gene names

Name:

FMO3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide. Ref.1
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity.
Subcellular location	Microsome membrane By similarity. Endoplasmic reticulum membrane By similarity.
Involvement in disease	Defects in FMO3 are the cause of a fishy off-flavor in cow milk due to by an elevated level of trimethylamine (TMA) in body fluids. Ref.1
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Dimethylaniline monooxygenase [N-oxide-forming] 3		PRO_0000147652
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	401	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8HYJ9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2CA5964889490CBA	FASTA	532	60,093
	10 20 30 40 50 60 MVKKVAIIGA GISGLASIRN CLEEGLEPTC FEKGEDIGGL WKFSDHVEEG RASIYRSVFT 70 80 90 100 110 120 NSSKEMTCFP DFPFPDDFPN FMHNSKLQEY ITMFAKEKNL LKYIQFKTIV SSVNKRPDFQ 130 140 150 160 170 180 TTGQWDVITE KDGKKESAVF DAVMICSGHH VYPNIPKESF PGIKLFKGKC FHSRDYKEPG 190 200 210 220 230 240 IFKGKRVLVI GLGNSGCDIA SELSHIAEKV IISSRSGSWV MSRVWDEGYP WDMLFITRFE 250 260 270 280 290 300 TFLKNTLPTV ISNWWYMKQM NARFKHENYG LMPLNSTLRK EPVFNDELPA CILCGIVTIK 310 320 330 340 350 360 PNVKEFTEDS AIFEDGTVFK AIDYVIFATG YSYAYPFLDD SIIKSRDNEV TLFKGIFPPP 370 380 390 400 410 420 LEKPTLAVIG LVQSLGAAIP TTDLQSRWAV QVIKGTCPLP SVKDMMNDID EKMGKKLKLF 430 440 450 460 470 480 GKSDTIQTDY VVYMDELASF IGAKPNIPWL FLTDPKLALE VYFGPCTPYQ FRLVGPGKWP 490 500 510 520 530 GARNAILTQW DRLLKPMTTR VVGSPLKPCL FCNWFRPVLI SVVSIAALIV LF

References

[1]	"A nonsense mutation in the FMO3 gene underlies fishy off-flavor in cow's milk." Lunden A., Marklund S., Gustafsson V., Andersson L. Genome Res. 12:1885-1888(2002) [PubMed: 12466292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISEASE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF488422 mRNA. Translation: AAN27919.1.
IPI	IPI00695492.
RefSeq	NP_776482.1.
UniGene	Bt.12519
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000020597. Bos taurus. [Contig view]
GeneID	281167.
KEGG	bta:281167.
Phylogenomic databases
HOVERGEN	Q8HYJ9.
Enzyme and pathway databases
BRENDA	1.14.13.8. 251.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002255. Flavin_mOase_3. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01123. FMOXYGENASE3.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

FMO3_BOVIN

Accession

Primary (citable) accession number: Q8HYJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)