Reviewed, UniProtKB/Swiss-Prot Q8HYJ5 (FUT3_PONPY)

Names and origin

Protein names

Recommended name:
    Galactoside 3(4)-L-fucosyltransferase
    EC=2.4.1.65
Alternative name(s):
    Blood group Lewis alpha-4-fucosyltransferase
      Short name=Lewis FT
    Fucosyltransferase 3
    Fucosyltransferase III
      Short name=FucT-III
    Alpha-3/4-fucosyltransferase

Gene names

Name:

FUT3

Organism

Pongo pygmaeus (Bornean orangutan)

Taxonomic identifier

9600 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of sialyl Lewis X and Lewis X/SSEA-1 antigens. It may be involved in blood group Lewis determination By similarity.
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Miscellaneous	Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Galactoside 3(4)-L-fucosyltransferase		PRO_0000221098
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 34	20	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 372	338	Lumenal Potential
Amino acid modifications
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8HYJ5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: DE08B961B0684CB8	FASTA	372	43,008
	10 20 30 40 50 60 MDPLGAAKTQ WPWRRCLAAL LFQLLVAVCF FSYLRVSRDD ATGSPRPGLM AVEPVTGAPG 70 80 90 100 110 120 GSSRQDTTPT RHTLLILLWT WPFHIPVALS RCSEMAPGTA DCHITADRKV YPQADAVIVH 130 140 150 160 170 180 HWDIMSNPKS RLPPSPRPQG QRWIWFSLEP PPNCQHLEAL DGYFNLTMSY RSDSDIFTPY 190 200 210 220 230 240 GWLEPWSGQP AHPPLNLSAK TELVAWAVSN WKPDSARVHY YQSLQAHLKV DVYGRSHKPL 250 260 270 280 290 300 PKGTMMETLS RYKFYLAFEN SLHPDYITEK LWRNALEAWA VPVVLGPSRS NYERFLPPDP 310 320 330 340 350 360 FIHVDDFQSP KDLARYLQEL DKDHARYLSY FRWRETLQPR SFSWALDFCK ACWKLQQESR 370 YQMVRSIAAW FT

References

[1]

"Structure/function study of Lewis alpha3- and alpha3/4-fucosyltransferases: the alpha1,4 fucosylation requires an aromatic residue in the acceptor-binding domain." Dupuy F., Germot A., Julien R., Maftah A. Glycobiology 14:347-356(2004) [PubMed: 14718375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF515438 Genomic DNA. Translation: AAO15994.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Genome annotation databases
Ensembl	ENSPPYT00000010999; ENSPPYP00000010584; ENSPPYG00000009438; Pongo pygmaeus. [Genome view]
Phylogenomic databases
HOVERGEN	Q8HYJ5.
Enzyme and pathway databases
BRENDA	2.4.1.65. 269192.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT3_PONPY

Accession

Primary (citable) accession number: Q8HYJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)